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Double-layer tissue engineering skin and preparation method thereof

A tissue engineering, double-layer technology, applied in the field of double-layer tissue engineering skin and its preparation, can solve the problems of high brittleness, large shrinkage rate of scaffold collagen gel, difficult operation, etc.

Active Publication Date: 2017-05-17
GUANGZHOU RAINHOME PHARM&TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dermagraft only has a single-layer tissue-engineered skin of the dermis; Apligraft contains both the epidermis and the tissue-engineered skin of the dermis, but the shrinkage rate of the scaffold collagen gel used is relatively large, and the use of allogeneic cells and bovine collagen has problems Anticipated risk of immune rejection and potential virus infection, high fragility and difficult operation

Method used

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  • Double-layer tissue engineering skin and preparation method thereof
  • Double-layer tissue engineering skin and preparation method thereof
  • Double-layer tissue engineering skin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] A tissue-engineered double-layer skin, which uses fibroblasts and epidermal cells as seed cells and decellularized amnion as a biological scaffold to construct the obtained tissue-engineered double-layer skin.

[0079] The preparation method of the tissue-engineered double-layered skin comprises the following steps:

[0080] a. Prepare decellularized amniotic membrane, culture medium and pluripotent stem cells

[0081] 1) Preparation of decellularized amniotic membrane

[0082] 1-1) Select the placenta of cesarean delivery women, prenatal serological examination to exclude hepatitis B, hepatitis C, syphilis and acquired immunodeficiency syndrome, wash the amniotic surface of the placenta after detection with sterile PBS, and separate the amniotic membrane and villi The potential gap between the membranes was bluntly peeled off the amniotic membrane, and the aforementioned materials were all collected under sterile conditions;

[0083] 1-2) Degreasing treatment of amni...

Embodiment 2

[0129] A tissue-engineered double-layer skin, which uses fibroblasts and epidermal cells as seed cells and decellularized amnion as a biological scaffold to construct the obtained tissue-engineered double-layer skin.

[0130] The preparation method of the tissue-engineered double-layered skin comprises the following steps:

[0131] a. Prepare decellularized amniotic membrane, culture medium and pluripotent stem cells

[0132] 1) Preparation of decellularized amniotic membrane

[0133] 1-1) Select the placenta of cesarean delivery women, prenatal serological examination to exclude hepatitis B, hepatitis C, syphilis and acquired immunodeficiency syndrome, wash the amniotic surface of the placenta after detection with sterile PBS, and separate the amniotic membrane and villi The potential gap between the membranes was bluntly peeled off the amniotic membrane, and the aforementioned materials were all collected under sterile conditions;

[0134] 1-2) Degreasing treatment of amni...

Embodiment 3

[0180] A tissue-engineered double-layer skin, which uses fibroblasts and epidermal cells as seed cells and decellularized amnion as a biological scaffold to construct the obtained tissue-engineered double-layer skin.

[0181] The preparation method of the tissue-engineered double-layered skin comprises the following steps:

[0182] a. Prepare decellularized amniotic membrane, culture medium and pluripotent stem cells

[0183] 1) Preparation of decellularized amniotic membrane

[0184] 1-1) Select the placenta of cesarean delivery women, prenatal serological examination to exclude hepatitis B, hepatitis C, syphilis and acquired immunodeficiency syndrome, wash the amniotic surface of the placenta after detection with sterile PBS, and separate the amniotic membrane and villi The potential gap between the membranes was bluntly peeled off the amniotic membrane, and the aforementioned materials were all collected under sterile conditions;

[0185] 1-2) Degreasing treatment of amni...

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Abstract

The invention discloses a tissue engineering double-layer skin and a preparation method thereof. The tissue engineering double-layer skin is characterized by being constructed by using fibroblast cells and epidermal cells as seed cells and an acellular amniotic membrane as a biological scaffold. The tissue engineering double-layer skin disclosed by the invention has the advantages of low cost, simple operation, wide sources and easy storage, and is a skin substitution having high transplantation efficiency. The invention also provides the preparation method of the tissue engineering double-layer skin.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a double-layer tissue engineered skin and a preparation method thereof. Background technique [0002] The skin is the largest organ and tissue of the human body, and it is the barrier between the body and the external environment. It has important functions such as protection, secretion, metabolism and sensation. However, when the skin is traumatized due to genetic factors, burns, diabetes, etc., the body's barrier function can be lost, causing infection, water and electrolyte imbalance, low immunity, and multiple organ failure. The most common method is auto-skin transplantation, but for large-area burns, the source of normal auto-skin is limited and the probability of skin survival after transplantation is low. If non-self-skin is used, the problem of immune rejection will also occur. [0003] Tissue engineering is a science that applies the principles and methods o...

Claims

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Application Information

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IPC IPC(8): A61L27/60A61L27/40A61L27/36A61L27/38A61L27/20A61L27/50
Inventor 黄燕飞车七石冼钢钟经德
Owner GUANGZHOU RAINHOME PHARM&TECH CO LTD
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