The invention provides a method for separating an
active ingredient, with an anti-oxidation effect, from human
umbilical cord mesenchymal stem cell culture fluid. The
active ingredient is prepared according to the following steps: in a process of normal
cesarean delivery operation or eutocia, taking an
umbilical cord under an aseptic condition, cleaning and disinfecting the
umbilical cord, then removing blood vessels and mucosae,
cutting umbilical cord tissues into pieces, and putting into a culture
bottle for culture; taking the
culture fluid of human umbilical cord mesenchymal stem cells growing in a log phase, and performing centrifugal
ultrafiltration by adopting
ultrafiltration centrifuge tubes with different specifications, thus finally obtaining an
outlier having the anti-oxidationeffect and the molecular weight of 10 to 30Kda. The
outlier can be freeze-dried into freeze-dried
powder, thus facilitating storage and use. The human umbilical cord
mesenchymal stem cell culture fluid outlier provided by the invention has good anti-
oxidation activity, can help cells
resist damage of free radicals; the outlier is from a
human body tissue, is high in safety, and is expected to be applied to products such as drugs, health care products,
skin care products and other daily chemicals. The
separation method provided by the invention is simple and easy, and is low-cost, free of environment
pollution and suitable for industrialized production.