Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for separating anti-oxidation active ingredient from human umbilical cord mesenchymal stem cell culture fluid

A technology of stem cells and active ingredients, applied in the direction of medical preparations containing active ingredients, animal cells, vertebrate cells, etc., can solve the problems of high virus infection rate, limited clinical wide application, easy pollution, etc., and achieve simple separation process , good cleaning effect, easy to operate

Inactive Publication Date: 2018-05-04
武汉北度生物科技有限公司
View PDF11 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, BMSCs are the most extensively researched. However, due to the extremely low level of BMSCs derived from human bone marrow and the high rate of virus infection, the potential of cell proliferation and differentiation decreases with age, and puncture is an invasive and traumatic process. , Contamination-prone operation, which limits its wide clinical application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for separating anti-oxidation active ingredient from human umbilical cord mesenchymal stem cell culture fluid
  • Method for separating anti-oxidation active ingredient from human umbilical cord mesenchymal stem cell culture fluid
  • Method for separating anti-oxidation active ingredient from human umbilical cord mesenchymal stem cell culture fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0030] The present invention will be further described in detail below in conjunction with the embodiments of the accompanying drawings.

[0031] The separation method and preparation process of antioxidant active components in human umbilical cord mesenchymal stem cell culture medium are as follows.

[0032] Step 1: preparation of human umbilical cord mesenchymal stem cells.

[0033] (1) Preservation of the umbilical cord: During normal cesarean section or vaginal delivery, the umbilical cord is taken under aseptic conditions, stored in a preservation solution, and stored at 4°C for later use.

[0034] (2) Cleaning of the umbilical cord: Take out the umbilical cord from the preservation solution and put it into a sterilized container, add 60 ml of normal saline, shake and rinse for 25 seconds, then transfer to another sterilized container; add 60 ml of metronidazole to the sterilized container Soak in the injection solution for 5 minutes, and then wash the umbilical cord aga...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention provides a method for separating an active ingredient, with an anti-oxidation effect, from human umbilical cord mesenchymal stem cell culture fluid. The active ingredient is prepared according to the following steps: in a process of normal cesarean delivery operation or eutocia, taking an umbilical cord under an aseptic condition, cleaning and disinfecting the umbilical cord, then removing blood vessels and mucosae, cutting umbilical cord tissues into pieces, and putting into a culture bottle for culture; taking the culture fluid of human umbilical cord mesenchymal stem cells growing in a log phase, and performing centrifugal ultrafiltration by adopting ultrafiltration centrifuge tubes with different specifications, thus finally obtaining an outlier having the anti-oxidationeffect and the molecular weight of 10 to 30Kda. The outlier can be freeze-dried into freeze-dried powder, thus facilitating storage and use. The human umbilical cord mesenchymal stem cell culture fluid outlier provided by the invention has good anti-oxidation activity, can help cells resist damage of free radicals; the outlier is from a human body tissue, is high in safety, and is expected to be applied to products such as drugs, health care products, skin care products and other daily chemicals. The separation method provided by the invention is simple and easy, and is low-cost, free of environment pollution and suitable for industrialized production.

Description

technical field [0001] The invention relates to a method for separating and preparing an anti-oxidation active ingredient with a molecular weight between 10KDa and 30KDa from human umbilical cord mesenchymal stem cell culture fluid, belonging to the field of cell and bioengineering. Background technique [0002] Antioxidants can weaken or eliminate free radical damage to the human body, or protect food from oxidative damage and deterioration. Scientific studies have shown that cancer, aging or other diseases are mostly associated with the production of excess free radicals. Research on anti-oxidation can effectively overcome the harm it brings, so anti-oxidation is listed as one of the research and development directions by manufacturers of pharmaceuticals, health care products, skin care products and other daily chemical manufacturers, and it is also one of the most important functional demands of the market . Chemically synthesized antioxidants, such as propyl gallate, d...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775C12P1/00A61K35/28A61P39/06A61K8/99A61Q19/08
CPCA61K8/99A61K35/28A61Q19/08C12N5/0668C12P1/00
Inventor 邓勇王余江振华
Owner 武汉北度生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com