Method for separating anti-oxidation active ingredient from human umbilical cord mesenchymal stem cell culture fluid

Abstract

The invention provides a method for separating an active ingredient, with an anti-oxidation effect, from human umbilical cord mesenchymal stem cell culture fluid. The active ingredient is prepared according to the following steps: in a process of normal cesarean delivery operation or eutocia, taking an umbilical cord under an aseptic condition, cleaning and disinfecting the umbilical cord, then removing blood vessels and mucosae, cutting umbilical cord tissues into pieces, and putting into a culture bottle for culture; taking the culture fluid of human umbilical cord mesenchymal stem cells growing in a log phase, and performing centrifugal ultrafiltration by adopting ultrafiltration centrifuge tubes with different specifications, thus finally obtaining an outlier having the anti-oxidationeffect and the molecular weight of 10 to 30Kda. The outlier can be freeze-dried into freeze-dried powder, thus facilitating storage and use. The human umbilical cord mesenchymal stem cell culture fluid outlier provided by the invention has good anti-oxidation activity, can help cells resist damage of free radicals; the outlier is from a human body tissue, is high in safety, and is expected to be applied to products such as drugs, health care products, skin care products and other daily chemicals. The separation method provided by the invention is simple and easy, and is low-cost, free of environment pollution and suitable for industrialized production.

Description

technical field [0001] The invention relates to a method for separating and preparing an anti-oxidation active ingredient with a molecular weight between 10KDa and 30KDa from human umbilical cord mesenchymal stem cell culture fluid, belonging to the field of cell and bioengineering. Background technique [0002] Antioxidants can weaken or eliminate free radical damage to the human body, or protect food from oxidative damage and deterioration. Scientific studies have shown that cancer, aging or other diseases are mostly associated with the production of excess free radicals. Research on anti-oxidation can effectively overcome the harm it brings, so anti-oxidation is listed as one of the research and development directions by manufacturers of pharmaceuticals, health care products, skin care products and other daily chemical manufacturers, and it is also one of the most important functional demands of the market . Chemically synthesized antioxidants, such as propyl gallate, d...

AI Technical Summary

Problems solved by technology

At present, BMSCs are the most extensively researched. However, due to the extremely low level of BMSCs derived from human bone marrow and the high rate of virus infection, the potential of cell proliferation and differentiation decreases with age, and puncture is an invasive and traumatic process. , Contamination-prone operation, which limits its wide clinical application

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