The invention relates to a seminal fluid collecting device of a bionic female cowfish model. The device comprises a bionic model constructed based on skeleton data of a real female Yangtze River cowfish, a
buoyancy adjusting device and a
gravity center adjusting device are arranged in an inner cavity of the bionic model, and floating, diving and posture balance of the model are achieved by controlling the water amount of a
water storage inner container and moving a balancing weight; the seminal fluid collection device is located in the model reproductive crack
simulation area, is provided with a false
vaginal canal with a water blocking valve, and is used for guiding and collecting seminal fluid; the
pheromone slow-release device is arranged at the
anus simulation position and can control the release of liquid
pheromone; and the micro camera is arranged at the head and is used for observing behaviors. According to the method, the active
mating behavior of the male guinea pigs is effectively stimulated through the multi-mode bionic design in combination with the autonomous movement ability, and a key
technical support is provided for genetic resource rescue storage and full-artificial breeding research of the Yangtze River guinea pigs.