The invention relates to a medicament for treating liver-kidney yin deficiency type anovulatory dysfunctional uterine bleeding, and a preparation method and application thereof. The medicament is prepared from the following raw material medicaments in part by weight: 190 to 290 parts of white paeony root, 270 to 370 parts of rehmannia root, 190 to 290 parts of yerbadetajo herb, 190 to 290 parts of glossy privet fruit, 190 to 290 parts of himalayan teasel root, 30 to 130 parts of donkey-hide glue, 190 to 290 parts of radix pseudo-ginseng, 430 to 530 parts of astragalus, 190 to 290 parts of cortex lycii radicis and 270 to 370 parts of cuttlebone. The preparation method comprises the following steps of: according to the weight part, adding alcohol into the white paeony root, the yerbadetajo herb, the glossy privet fruit, the himalayan teasel root, the radix pseudo-ginseng and the astragalus for reflux and extraction, filtering, recovering the alcohol from the alcohol extract under reduced pressure, and concentrating the alcohol extract until the alcohol extract has no alcohol odor so as to obtain alcohol extract concentrated solution and decoction dregs for later use; and merging the decoction dregs with radix rehmanniae praeparata, the cortex lycii radicis and the cuttlebone, decocting the mixture by using water, filtering decoction, concentrating filter liquor under reduced pressure, adding the alcohol into the concentrated filter liquor, uniformly stirring, standing, filtering, recovering the alcohol from the filter liquor to obtain concentrated solution of water extraction and alcohol precipitation, merging the concentrated solution of water extraction and alcohol precipitation with the alcohol extract concentrated solution, adding the molten donkey-hide glue into the mixture, concentrating and performing vacuum drying to prepare dry extract powder.