Application of oxyresveratrol in preparing ovary aging resistant medicine

A technology for oxidizing resveratrol and ovarian aging, which is used in drug combinations, active ingredients of hydroxyl compounds, endocrine system diseases, etc.

Inactive Publication Date: 2013-05-01
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Application of oxyresveratrol in preparing ovary aging resistant medicine
  • Application of oxyresveratrol in preparing ovary aging resistant medicine
  • Application of oxyresveratrol in preparing ovary aging resistant medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] [Example 1] Proliferation effect of oxidized resveratrol on isolated ovarian granulosa cells (MTT method)

[0019] Female rats aged 22-27 days were killed by cervical dislocation after 48 hours of routine feeding. The bilateral ovaries of the rats were aseptically removed, and the ovaries were crushed to release granulosa cells. They were treated with 2 mL of DMEM-F12 containing penicillin and streptomycin. Dilute in culture medium and gently pipette to disperse into single cells. Pellet cells were collected by centrifugation. A certain concentration of cell suspension was inoculated in a 24-well plate, 80 μl per well, and 120 μl of culture medium was added to each well to adjust the cell density to 200 μl / well, and 6 parallel wells were set up for each group. Put it in 5% CO 2 Cultivate in the incubator for 24h. Take CO 2 The granule cell suspension was cultivated in the incubator for 24 hours, and each well was successively added with cadmium chloride at a modelin...

Embodiment 2

[0023] [Example 2] The effect of oxidized resveratrol on the estrogen and progesterone secretion of isolated ovarian granulosa cells (radioimmunization method)

[0024] Female rats aged 22-27 days were killed by cervical dislocation after 48 hours of routine feeding. The bilateral ovaries of the rats were aseptically removed, and the ovaries were broken to release granulosa cells. They were treated with 2ml of DMEM-F12 containing penicillin and streptomycin. Dilute in culture medium and gently pipette to disperse into single cells. Pellet cells were collected by centrifugation. A certain concentration of cell suspension was inoculated in a 24-well plate, 80 μl per well, and 120 μl of culture medium was added to each well to adjust the cell density to 200 μl / well, and 6 parallel wells were set up for each group. Put it in 5% CO 2 Cultivate in the incubator for 24h. Take CO 2The granule cell suspension was cultivated in the incubator for 24 hours, each well was successively ...

Embodiment 3

[0028] [Example 3] Effect of oxidized resveratrol on the secretion of estrogen and progesterone in natural aging rats (radioimmunoassay)

[0029] Adopt the pre-aging model of natural aging. Select 14-month-old female SD rats with a body weight of (270±30) g. After the animals are fed for one week, do vaginal cytology smears and observe 4 estrous cycles continuously. Vaginal cytology shows that the estrous cycle is prolonged and then continues to be in estrus , Repeated pseudo-pregnancy cell phase, as a successful model of female aging. Natural aging rats were randomly divided into model group, oxidized resveratrol (3mg / kg, 6mg / kg, 12mg / kg) group and positive drug group, with 10 rats in each group, and 11 female rats aged 4 months. Only for the normal control group, intragastric administration once a day. The normal control group and the model group were given equal volumes of normal saline. After 30 days of continuous administration, blood was collected from the orbital venou...

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Abstract

The invention discloses an application of oxyresveratrol in preparing an ovary aging resistant medicine. The oxyresveratrol has significant treatment effects on various ovary aging diseases such as polycystic ovarian syndrome, dysfunctional uterine bleeding, premature ovarian failure and climacteric syndrome. The oxyresveratrol is prepared into the oral, transdermal or injection medicine for treating the ovary aging diseases.

Description

technical field [0001] The invention relates to the medical use of oxidized resveratrol, that is, the application of oxidized resveratrol in drugs for effectively treating various ovarian decline diseases. Background technique [0002] Ovarian aging is a complex biological process with multiple factors interacting and gradually accumulating. The main cause of ovarian aging is the decline in the number of follicles as the number of follicles passes by. The accumulation of metabolites in the body changes the microenvironment of the ovary, such as damage to follicles by free radicals and end products of glycation, and mitochondrial DNA mutations, With the continuous accumulation of factors such as telomere shortening, the quality of remaining follicles is also reduced; the expression and function of a series of genes in the ovary are changed, and the secretion of H-P-O axis (Hypothalamus-Pituitary-Ovary Axis, hypothalamus-pituitary-ovary axis) hormones Finely regulate ovarian ...

Claims

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Application Information

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IPC IPC(8): A61K31/05A61P15/00A61P15/12A61P5/30
Inventor 韦敏吕晔
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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