Application of morindone in preparation of anti-ovarian senescence drugs

An ovarian aging and drug technology, applied in the direction of drug combination, ketone active ingredients, sexual diseases, etc., to increase the secretion of progesterone, expand the scope of application, and improve the secretion level of progesterone.

Pending Publication Date: 2020-03-24
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report yet about the application of Kuwanone in the preparation of anti-ovarian aging drugs

Method used

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  • Application of morindone in preparation of anti-ovarian senescence drugs
  • Application of morindone in preparation of anti-ovarian senescence drugs
  • Application of morindone in preparation of anti-ovarian senescence drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] [Example 1] The proliferative effect of Kuwanone on isolated ovarian granulosa cells (MTT method)

[0018] Female rats aged 22-27 days were killed by cervical dislocation after 48 hours of routine feeding. The bilateral ovaries of the rats were aseptically removed, and the ovaries were broken to release granulosa cells. Dilute in F12 medium and gently pipette to disperse into single cells. Pellet cells were collected by centrifugation. A certain concentration of cell suspension was inoculated in a 24-well plate, 80 μl per well, and 120 μl of culture medium was added to each well to adjust the cell density to 200 μl / well, and 6 parallel wells were set up for each group. Put it in 5% CO 2 Cultivate in the incubator for 24h. Take CO 2 The granule cell suspension was cultivated in the incubator for 24 h, and each well was successively added with cadmium chloride and different concentrations of Kuwanone samples (2.5 mg·mL -1 , 0.5mg·mL -1 , 0.1mg·mL -1 , 0.02mg·mL -1...

Embodiment 2

[0019] [Example 2] Effects of Kuwanone on Estrogen and Progesterone Secretion of Isolated Ovary Granulosa Cells (Radioimmunoassay)

[0020] Female rats aged 22-27 days were sacrificed by cervical dislocation after 48 hours of routine feeding. The bilateral ovaries of the rats were aseptically removed, and the ovaries were crushed to release granulosa cells. They were treated with 2ml of DMEM containing penicillin and streptomycin- Dilute in F12 medium and gently pipette to disperse into single cells. Pellet cells were collected by centrifugation. A certain concentration of cell suspension was inoculated in a 24-well plate, 80 μl per well, and 120 μl of culture medium was added to each well to adjust the cell density to 200 μl / well, and 6 parallel wells were set up for each group. Put it in 5% CO 2 Cultivate in the incubator for 24h. Take CO 2 The granule cell suspension was cultivated in the incubator for 24 hours, each hole was added with cadmium chloride and different ...

Embodiment 3

[0021] [Example 3] Effect of Kuwanon on the secretion of estrogen and progesterone in natural aging rats (radioimmunization method)

[0022]Adopt the pre-aging model of natural aging. Select 14-month-old female SD rats with a body weight of (270±30) g. After the animals are fed for one week, do vaginal cytology smears and observe 4 estrous cycles continuously. Vaginal cytology shows that the estrous cycle is prolonged and then continues to be in estrus , Repeated pseudo-pregnancy cell phase, as a successful model of female aging. The natural aging rats were randomly divided into model group, Kuwanone (10mg / kg, 30mg / kg, 50mg / kg) group and positive drug group, 10 in each group, and another 11 female rats aged 4 months were taken as normal The control group was intragastrically administered once a day, and the normal control group and the model group were given equal volumes of normal saline. After 30 days of continuous administration, blood was collected from the orbital veno...

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Abstract

The invention discloses an application of morindone in preparation of anti-ovarian senescence drugs. The morindone has a remarkable effect on treating various ovarian recession diseases such as polycystic ovarian syndrome, dysfunctional uterine bleeding, premature ovarian failure or menopausal syndrome by up-regulating the protein levels of c-fos mRNA and expression products thereof. According tothe invention, the morindone is prepared into oral administration, transdermal administration or injection administration for treating ovarian recession diseases.

Description

technical field [0001] The invention relates to the medical application of Kuwanone, that is, the application of Kuwanone in the medicine for effectively treating various ovarian decline diseases. Background technique [0002] Ovarian aging is a complex biological process with multiple factors interacting and gradually accumulating. The main cause of ovarian aging is the decline in the number of follicles as the number of follicles passes by. The accumulation of metabolites in the body changes the microenvironment of the ovary, such as damage to follicles by free radicals and end products of glycation, and mitochondrial DNA mutations, With the continuous accumulation of factors such as telomere shortening, the quality of remaining follicles is also reduced; the expression and function of a series of genes in the ovary are changed, and the secretion of H-P-O axis (Hypothalamus-Pituitary-Ovary Axis, hypothalamus-pituitary-ovary axis) hormones Finely regulate ovarian function....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/12A61P15/08A61P15/12
CPCA61K31/12A61P15/08A61P15/12
Inventor 钱怡云韦敏宋萍萍颜露吕晔
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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