Application of 5-hydroxymethylfurfural in preparation of anti-ovarian senescence drugs

A technology for hydroxymethylfurfural and ovarian aging, applied in the application field of 5-hydroxymethylfurfural in the preparation of anti-ovarian aging drugs, to improve the secretion level of estrogen and progesterone, increase the secretion of estrogen and progesterone, and expand the scope of application Effect

Inactive Publication Date: 2016-10-26
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on the application of 5-hydroxymethy

Method used

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  • Application of 5-hydroxymethylfurfural in preparation of anti-ovarian senescence drugs
  • Application of 5-hydroxymethylfurfural in preparation of anti-ovarian senescence drugs
  • Application of 5-hydroxymethylfurfural in preparation of anti-ovarian senescence drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] [Example 1] Proliferation effect of 5-hydroxymethylfurfural on isolated ovarian granulosa cells (MTT method)

[0019] Female rats aged 22-27 days were killed by cervical dislocation after 48 hours of routine feeding. The bilateral ovaries of the rats were aseptically removed, and the ovaries were broken to release granulosa cells. Dilute in F12 medium and gently pipette to disperse into single cells. Pellet cells were collected by centrifugation. A certain concentration of cell suspension was inoculated in a 24-well plate, 80 μl per well, and 120 μl of culture medium was added to each well to adjust the cell density to 200 μl / well, and 6 parallel wells were set up for each group. Put it in 5% CO 2 Cultivate in the incubator for 24h. Take CO 2 The granule cell suspension was cultivated in the incubator for 24 h, and each well was successively added with cadmium chloride at a modeling concentration and 5-hydroxymethylfurfural samples at different concentrations (1.0 m...

Embodiment 2

[0022] [Example 2] The effect of 5-hydroxymethylfurfural on the secretion of estrogen and progesterone in isolated ovarian granulosa cells (radioimmunization method)

[0023] Female rats aged 22-27 days were sacrificed by cervical dislocation after 48 hours of routine feeding. The bilateral ovaries of the rats were aseptically removed, and the ovaries were crushed to release granulosa cells. They were treated with 2ml of DMEM containing penicillin and streptomycin- Dilute in F12 medium and gently pipette to disperse into single cells. Pellet cells were collected by centrifugation. A certain concentration of cell suspension was inoculated in a 24-well plate, 80 μl per well, and 120 μl of culture medium was added to each well to adjust the cell density to 200 μl / well, and 6 parallel wells were set up for each group. Put it in 5% CO 2 Cultivate in the incubator for 24h. Take CO 2 The granule cell suspension was cultivated in the incubator for 24 hours, each hole was added wit...

Embodiment 3

[0026] [Example 3] The effect of 5-hydroxymethylfurfural on the secretion of estrogen and progesterone in natural aging rats (radioimmunization method)

[0027] Adopt the pre-aging model of natural aging. Select 14-month-old female SD rats with a body weight of (270±30) g. After the animals are fed for one week, do vaginal cytology smears and observe 4 estrous cycles continuously. Vaginal cytology shows that the estrous cycle is prolonged and then continues to be in estrus , Repeated pseudo-pregnancy cell phase, as a successful model of female aging. Natural aging rats were randomly divided into model group, 5-hydroxymethylfurfural (10mg / kg, 30mg / kg, 50mg / kg) group and positive drug group, with 10 rats in each group, and another 4-month-old female rat The 11 rats were the normal control group, which were intragastrically administered once a day. The normal control group and the model group were given equal volumes of normal saline. After 30 days of continuous administration, ...

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Abstract

The invention discloses application of 5-hydroxymethylfurfural in the preparation of anti-ovarian senescence drugs. 5-Hydroxymethylfurfural can treat various ovarian failures significantly effectively, such as polycystic ovarian syndrome, dysfunctional uterine bleeding, premature ovarian failure or climacteric syndrome. The 5-hydroxymethylfurfural is made herein into oral drugs, transdermal drugs or injectable drugs for treating ovarian senescence.

Description

technical field [0001] The invention relates to the medical use of 5-hydroxymethylfurfural, that is, the application of 5-hydroxymethylfurfural in effective medicines for treating various ovarian recession diseases. Background technique [0002] Ovarian aging is a complex biological process with multiple factors interacting and gradually accumulating. The main cause of ovarian aging is the decline in the number of follicles as the number of follicles passes by. The accumulation of metabolites in the body changes the microenvironment of the ovary, such as damage to follicles by free radicals and end products of glycation, and mitochondrial DNA mutations, With the continuous accumulation of factors such as telomere shortening, the quality of remaining follicles is also reduced; the expression and function of a series of genes in the ovary are changed, and the secretion of H-P-O axis (Hypothalamus-Pituitary-Ovary Axis, hypothalamus-pituitary-ovary axis) hormones Finely regulat...

Claims

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Application Information

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IPC IPC(8): A61K31/341A61P15/00A61P15/12
CPCA61K31/341
Inventor 韦敏宋萍萍窦剑郑生智吕晔
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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