55 results about "Membrane cell" patented technology

The present invention relates to a method of producing cells having the phenotype of cardiac valve cells from precursor cells of valve cells comprising (a) introducing into said precursor cells early growth response 3 (EGR3) and / or a nucleic acid sequence encoding EGR3. The present invention also relates to a valve transplant comprising cells having the phenotype of valve cells obtained or obtainable by the method of the invention. The present invention furthermore relates to cells having the phenotype of valve cells obtained or obtainable by the method of the invention, the valve transplant of the invention or a compound promoting the expression and / or the activity of early growth response 3 (EGR3) for use in treating a subject having a valve defect.

Single channel liquid membrane cell assemblies and cell bodies are disclosed. In some embodiments, the single channel liquid membrane cell assembly includes an elongate cell body having an elongate opening, a first bipolar plate adjacent the cell body, and a first gas diffusion electrode disposed between the cell body and the first bipolar plate. The first gas diffusion electrode spans across the entire length and width of the elongate opening of the cell body. The single channel liquid membrane cell assembly additionally includes a second bipolar plate adjacent the cell body such that the cell body is disposed between the first and second bipolar plates. The elongate opening horizontally defines an open area and the first gas diffusion electrode and the second bipolar plate vertically define the open area therebetween.

The utility model discloses an air-cooled hydrogen fuel cell bipolar plate, a single cell and an electric pile, and belongs to the technical field of air-cooled hydrogen fuel cells. The bipolar plate comprises a plate body, a first groove is formed in one side face of the plate body, a cathode runner is arranged in the first groove, a second groove is formed in the other side face of the plate body, an anode runner is arranged in the second groove, and an air inlet and an air outlet are formed in the two ends of the plate body respectively. And the air inlet and the air outlet are respectively communicated with two ends of the anode runner. The cathode flow channel adopts a parallel flow channel; and the anode runner adopts a snakelike runner. The anode flow channel and the cathode flow channel of the bipolar plate are both arranged in the sunken open groove, and the compression amount of the gas diffusion layer is controlled according to the depth of the open groove; according to the structure, the problem that a gas diffusion layer in a traditional membrane battery is prone to overpressure can be effectively prevented, so that the diffusion rate and uniformity of hydrogen and oxygen between the membrane electrodes are improved, and the response speed and the output performance of the single-frame membrane electrode are further improved.

To provide a method for evaluating the quality of neuroretina for transplantation and neuroretina sheets for transplantation selected by said method. [Solution] The present invention provides a method for evaluating the quality of neuroretina for transplantation, comprising: extracting a part or all of a cell aggregate containing a neuroretina having an epithelial structure derived from pluripotent stem cells as a quality evaluation sample; detecting the expression of neuroretinal cell-related genes and non-neuroretinal cell-related genes in the quality evaluation sample; and determining, if the expression of neuroretinal cell-related genes is observed and the expression of non-neuroretinal cell-related genes is not observed, that (1) a neuroretina (neuroretina for transplantation) from the same cell aggregate as the cell aggregate containing a part of the quality evaluation sample, (2) a neuroretina (neuroretina for transplantation) from the same lot as the cell aggregate containing a part of the quality evaluation sample, or (3) a neuroretina (neuroretina for transplantation) from the same lot as the cell aggregate of the entire quality evaluation sample, wherein the non-neuroretinal cell-related genes include one or more genes selected from the group consisting of brain and spinal cord tissue marker genes and eyeball-related tissue marker genes.

This invention discloses a single-cell spatial annotation method that integrates image and sequencing data. It obtains candidate cue point locations, cell nucleus instance segmentation masks, and cell nucleus type probability vectors by processing full-field H&E stained tissue images. It also obtains a preprocessed spatial transcriptome raw sequencing matrix and corresponding cell type labels by processing the raw spatial transcriptome sequencing matrix. Each capture site and its corresponding integrated data structure are constructed. Based on rigid constraints on the number of cells allocated to each type within each capture site and main type quota constraints, the main type annotation is written. For cells in the intercapture zone, one or more neighboring capture sites with existing annotation results are found. The main type and cell subtype of the intercapture zone cells are inferred through morphological feature similarity calculation, thus obtaining the annotation result. This solves the problem that existing technologies cannot determine the exact type of each cell in ST-sequencing tissue.

The application discloses a kind of corneal near physiological biomechanics intelligent simulation method and device, it is related to biomechanics and biomechanics experimental device technical field, the present application includes mutually connected force load cavity, force loading component, sensor monitoring feedback component etc..The present application can realize to corneal repair material, corneal cell, corneal cell-material compound loading individual and compound flow shear force, static pressure, dynamic pressure, static tension and dynamic tension, gas-liquid dynamic culture is simultaneously carried out, and through pressure and flow sensor, the stress size of loading is monitored in real time, reach accurate intelligent comprehensive simulation cornea in body environment.The scheme provided by the present application can be used for the mechanical property detection of corneal tissue and material, obtains the material attribute under near physiological state;It can also be used for the mechanical loading of cell, to observe the cell behavior and functional change after near physiological mechanical loading.

The utility model belongs to the technical field of medical instruments, and particularly relates to a reusable heavy ion microporous membrane cell filtering device which comprises a lower cylinder component, comprising a lower cylinder main body, an extension part arranged at the top of the lower cylinder main body, an external thread arranged on the surface of the extension part, a mounting groove formed in the top of the extension part, and a heavy ion microporous membrane main body arranged in the mounting groove; the upper barrel assembly comprises an upper barrel main body arranged at the top of the lower barrel main body, an internal thread arranged at the bottom of the inner side wall of the upper barrel, and a limiting ring arranged on the inner side wall of the upper barrel; the structure is reasonable, in the using process, the heavy ion microporous membrane is convenient to replace, repeated use is convenient, the detection cost is reduced, and meanwhile generation of medical waste is reduced.

The utility model relates to the technical field of water treatment, in particular to a membrane pool water treatment system which comprises a membrane pool provided with a plurality of galleries, a medicament storage tank, a dosing pump, a dosing main pipeline, a plurality of branch pipelines and a controller, water inlet flow meters are arranged at the water inlet ends of the galleries; a first flow regulating valve is arranged on each branch pipeline; and the water inlet flow meter, the first flow regulating valve and the dosing pump are all in signal connection with the controller. According to the utility model, the defect in the prior art that the dosage cannot be respectively and accurately added manually according to the actual water quantity of each gallery is overcome, and the actual water quantity of the gallery can be measured by the water inlet flow meter, the medicament storage tank, the dosing pump, the first flow regulating valve and the controller; the controller can control the start and stop of the dosing pump and the opening degree of the first flow regulating valve according to the actual water quantity, so that the dosing amount of the medicament in each gallery is accurately controlled, the medicament is prevented from being excessive or insufficient, the flocculation effect can be ensured, the waste of the medicament can be avoided, and the medicament cost is saved.

The application discloses a dislocation tooth storage and treatment box and a storage and treatment method. The box comprises a box body and a box cover. One side of the box cover is hinged to the box body, and the other side of the box cover is detachably connected to the box body. The box body is internally provided with a cleaning groove, a liquid storage groove, a tooth storage groove and a storage groove. The cleaning groove and the tooth storage groove penetrate through the bottom of the box body, and a sealing plug is detachably connected to the bottom of the box body. The cleaning groove, the liquid storage groove and the tooth storage groove are respectively detachably connected with a cleaning groove cover, a liquid storage groove cover and a tooth storage groove cover, which are fixedly connected to the box cover. A pair of tweezers is detachably connected to the storage groove. The scheme uses physiological saline to flush dirt on the dislocation tooth, uses HBSS to maintain and recombine cell components consumed by periodontal membrane cells, provides a good storage environment, uses HBSS containing ALN to treat the dislocation tooth to reduce root absorption after replantation, avoid bone adhesion and improve the replanting success rate.

The invention discloses a method for calculating a diffusion coefficient of micro-pollutants in a nanofiltration membrane based on adsorption capacity in the technical field of membranes, which comprises the following steps: installing the nanofiltration membrane in a membrane pool filled with a salt solution, dissolving the micro-pollutants in the salt solution, and intercepting and adsorbing the micro-pollutants by the nanofiltration membrane in the membrane pool; and soaking the nanofiltration membrane which adsorbs the micro-pollutants in an elution solution to desorb the micro-pollutants. According to the method, the adsorption capacity of the micropollutants in the nanofiltration membrane is obtained through conventional micropollutant interception, adsorption and desorption experiments, the diffusion coefficient can be calculated, the testing method is simple, the mutual relation between the micropollutants and the membrane and the mutual relation between the micropollutants and the solvent are considered in the mass transfer model calculation process, and the calculation accuracy is high. The influence of the complex interaction force of the membrane-micropollutants on the mass transfer process of the micropollutants is introduced, so that the calculation of the diffusion coefficient of the micropollutants except ions in the membrane is more accurate.

The invention belongs to the technical field of medicines, and particularly relates to application of a licorice extract in preparation of a medicine for treating premature ovarian failure. On the basis of network pharmacology prediction and animal experiment verification, through experimental exploration, it is clear that diammonium glycyrrhizinate can treat premature ovarian failure by improving ovarian tissue energy metabolism, reducing the oxidative stress level and inhibiting ovarian cell apoptosis, and also can treat premature ovarian failure by improving the ovarian growth and development microenvironment. According to the present invention, the growth and development rate of ovarian granular cells and membrane cells is improved, the sufficient quantity of granular cells and membrane cells are ensured to secrete female progestational hormone, the damaged ovarian tissue is further protected, the new strategy and hope are provided for the treatment of premature ovarian failure, and the pharmaceutical composition is expected to become the safe and effective treatment drug.

The utility model discloses a cell culture carrier capable of recycling cells, and relates to the technical field of exosome production and cell recycling, the cell culture carrier comprises a cell growth layer used for cell growth, the outer wall of the cell growth layer is provided with a thin film, the outer wall of the thin film is provided with a thick film, and the thick film is provided with a plurality of cells. According to the cell culture carrier capable of recycling the cells, after cell amplification reaches a plateau phase, 10%-90% of intra-membrane cells can dissociate and fall off from the side wall of a membrane by increasing the rotating speed without a digestion process, and the viability of the free cells is greater than 80%; the purpose of cell recovery can be achieved through operations such as centrifugation, and the cell amplification and recovery process is greatly simplified; the intra-membrane cells can be continuously amplified and harvested by continuously facilitating falling and updating, meanwhile, the yield per unit volume of the exosome after the cell growth layer is improved is also improved to a certain extent compared with the yield of the exosome of a common structure (two layers of membranes and one layer of spacing net) in the market, and the total yield can be improved through multi-batch harvesting.

1. The name of the design product: gas-liquid pipeline (gas permeable membrane cell culture device). 2. The use of the design product: gas-liquid pipeline for connecting gas permeable membrane cell culture device. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The invention belongs to the technical field of electrolytic synthesis, and particularly relates to a method for preparing formic acid through three-chamber two-membrane electrolysis of CO2. The method is based on a three-chamber two-membrane electrolysis device which is divided into an anode chamber, a middle chamber and a cathode chamber by a cationic membrane and a nanofiltration membrane, a concentrated KOH aqueous solution is added into the anode chamber, a dilute KOH solution is added into the middle chamber, and CO2 and water are introduced into the cathode chamber; and after a power supply is switched on for full reaction, dilute KOH solutions are obtained in the anode chamber and the intermediate chamber respectively, and a KCOOH product is obtained in the cathode chamber. According to the method, the utilization rate of CO2 is remarkably increased, the electrolysis power consumption is reduced, the problems of water logging and the like are avoided, and the operation life is greatly prolonged.

An object of the present invention is to provide a cultured cell sheet that can solve problems accompanied by defects of mucosal tissue, such as inflammation in mucosal tissues covering bone tissues and granulation tissue formation. The present inventors have found that a cultured cell sheet obtained by culturing cells collected from a nasal mucosal tissue as a source under specific conditions, and reconstructing the cells into a sheet are engrafted onto mucosal tissue defective sites; promote the regeneration of mucosal tissues; efficiently reduce inflammation that occurs in a middle ear bone tissue; and prevent fibrosis and the like that are characteristic of bone tissue inflammation, thereby demonstrating that the cultured cell sheet can solve the problems. It is also found that the cultured cell sheet of the present invention is also useful for preventing inflammation of bone tissues in areas other than the middle ear.

The utility model provides a tangential flow membrane pool structure which comprises a membrane pool assembly, the membrane pool assembly is located on a pressing assembly, a press machine is installed at the bottom of the pressing assembly, and the membrane pool assembly comprises an upper-layer membrane pool structure, a middle-layer membrane pool structure, a lower-layer membrane pool structure, a plurality of filtering membranes and a plurality of membrane supporting pieces. The tangential flow membrane pool structure has the beneficial effects that the tangential flow membrane pool structure is a layered structure, the layered structure is detachable, and the sectional area of a single layer is larger; multiple layers can be stacked for use; meanwhile, the high sealing performance and the high pressure resistance are achieved through the pressing assembly; and the pressing assembly is also convenient to operate.

The invention belongs to the technical field of proton exchange membrane cells, and discloses a cathode graphite polar plate for a fuel cell, which comprises a public channel, a gas distribution area and an active area, a hydrogen inlet public channel, a cooling water inlet public channel, an air inlet public channel, a hydrogen outlet public channel, a cooling water outlet public channel, an air outlet public channel, an air inlet distribution port, an air outlet distribution port, a positioning hole, a gas distribution area and a three-dimensional channel with a contraction and expansion structure are arranged on the cathode graphite polar plate. The cathode graphite polar plate channel has the characteristic of combining a three-dimensional waveform structure and a shrinkage-expansion structure. The runners on the cathode graphite polar plate are designed in a staggered manner. And the spacing, the arrangement mode and the flow guide angle of the flow guide plates are optimized in the distribution area, so that gas is uniformly distributed to each channel of the bipolar plate, and the performance and the efficiency of the fuel stack are favorably improved. Cathode graphite polar plate channels are all formed by carving, so that the processing is convenient, and the batch production is facilitated.

Liquid membrane cell assemblies are disclosed. In some embodiments, the liquid membrane cell assembly includes an elongate base having opposed first and second end portions and a central portion disposed therebetween. The first and second end portions each includes an elongate body, an electrolyte channel within the body, an electrolyte port fluidly connected to the electrolyte channel, a fuel channel within the body, and a fuel port fluidly connected to the fuel channel. The central portion includes spaced and opposed first and second members that connect the bases of the first and second portions and that horizontally define an open area therebetween. The liquid membrane cell assembly additionally includes an anode adjacent the first and second members, and a cathode adjacent the first and second members such that the base is disposed between the anode and the cathode. The anode and cathode vertically define the open area therebetween.

The invention relates to a preparation method of high-purity methanol, and belongs to the technical field of methanol generation, the preparation method of the high-purity methanol comprises the following operation steps: placing a modified polyethersulfone microfiltration membrane in a membrane pool, introducing crude methanol into the membrane pool, and filtering to obtain the high-purity methanol, the flow velocity of the crude methanol in the membrane pool purification process being 1-3 L / min, and the permeation side pressure being 10-60 kPa; the preparation method of the modified polyethersulfone microporous filter membrane comprises the following steps: preparing a membrane by using a phase inversion method, adding Zn-MOF-74 into DMAc, adding polyethersulfone after complete dispersion to obtain a membrane casting solution, casting the prepared membrane casting solution on a smooth glass plate, and performing vacuum drying to obtain the modified polyethersulfone microporous filter membrane. The membrane separation technology is adopted, the methanol separation and purification efficiency is effectively improved on the premise that equipment investment is not increased and purification energy consumption is saved, and the content of the prepared methanol can reach 99.99% or above.

The utility model relates to the technical field of cell culture devices, and provides a gas-permeable membrane cell culture bottle, which at least comprises a bottle bottom provided with a first clamping body; the bottle bottom fixing frame is arranged outside the bottle bottom in a sleeving mode, a second clamping body matched with the first clamping body is arranged on the bottle bottom fixing frame, and during assembling, the first clamping body and the second clamping body are clamped with each other so that the bottle bottom can be prevented from falling off from the bottle bottom fixing frame. According to the breathable membrane cell culture bottle, the first clamping body is designed at the bottle bottom, the second clamping body is arranged at the corresponding position of the bottle bottom fixing frame, and during assembly, the first clamping body and the second clamping body are clamped with each other, so that stable connection between the bottle bottom and the bottle bottom fixing frame is ensured, and the bottle bottom is prevented from falling off from the bottle bottom fixing frame.

The invention is applicable to the technical field of cell models, and provides a method for establishing a porcine nasal mucosa M cell differentiation model, which comprises the following steps: preparing porcine nasal mucosa epithelial cells; construction of a pGEX-6p-1-RANKL recombinant expression vector, and prokaryotic expression of the recombinant expression vector; inducing the porcine nasal mucosa epithelial cells to differentiate into M cells by the porcine RANKL; and RT-qPCR (real-time quantitative polymerase chain reaction) is used for detecting the expression conditions of M cell differentiation related genes Spi-B, Tnfaip2 and GP2. According to the invention, the functional pig source RANKL recombinant protein is successfully constructed, and pig nasal mucosa epithelial cells are successfully induced to be differentiated into M cells by using the functional pig source RANKL recombinant protein. Through an RT-qPCR experiment, the expression level of M cell related genes is accurately detected, and the differentiation efficiency is verified. The achievement not only provides a tool for research on porcine respiratory diseases and development of drug vaccines, but also promotes research on cross-species mucosal immunity, and provides a new perspective and an experimental tool for the field of mucosal immunity.

The invention relates to the technical field of biomedicine, in particular to a method for promoting formation of nerve fasciae and repair and regeneration after sciatic nerve defect by using small extracellular vesicles (hfNCSCs-sEVs) derived from hair follicle neural crest stem cells and application. According to the application disclosed by the invention, the effects of the hfNCSCs-sEVs on proliferation, migration, tube formation and barrier formation of nerve fascia cells in vitro are defined; the effect of hfNCSCs-sEVs on tissue regeneration and function recovery after rat sciatic nerve defect is defined, the hfNCSCs-sEVs inhibits expression of Smad7 in nerve tract membrane cells by transferring miR-21-5p, activates a TGF-beta / Smad signal channel, up-regulates expression of HAS2, promotes proliferation and migration of HAS2 and promotes expression of tight junction protein thereafter, and the hfNCSCs-sEVs can be used for treating rat sciatic nerve defect. And a new view angle and theoretical support are provided for repair and regeneration of sciatic nerve injury.

The application discloses a kind of electroactive microorganism detection method and kit.The application utilizes the catalytic action of electroactive microorganism extracellular electron transfer key protein (outer membrane cytochrome c) and chemiluminescence technology, combined with the photographing function of smart phone, picture information processing technology, provide a kind of electroactive microorganism detection method and detection kit.No need to use any nanomaterial or special equipment, by commonly used smart phone and computer application program can realize signal acquisition, processing, calculation and result output;Convenient and fast, good stability, low cost, simple operation, the whole detection process can be completed without professional training;And response speed is fast, the whole detection process can be completed in 2min;It can be widely used in field detection.

The invention concerns a caustic concentration and flaking plant comprising a caustic solution feeding line, a first stage evaporator (EV-2101) receiving membrane cell liquor with a 30 to 50 wt.- % caustic from the feeding line and operating at a reduced pressure of 80 to 120 mbar, a second stage evaporator (EV-2201) receiving enriched caustic solution with 45 to 60 wt.-% from the first evaporator (EV-2101) and operating at atmospheric pressure, a third stage evaporator (EV-2301) receiving further enriched caustic solution with 83 to 89 wt.-% caustic from the second stage evaporator (EV-2201) and operating at a reduced pressure of 120 to 210 mbar for KOH and 550 to 700 mbar for NaOH, the first stage evaporator (EV-2101) being heated by the vapours generated by the second stage evaporator (EV-2201), the second stage evaporator (EV-2201) and the third stage evaporator (EV2301) being heated by thermal oil provided by a heater (H- 6301), a closed loop with a circulation pump (P-6301) for circulating thermal oil between the heater (H-6301), and the second and third stage evaporators (EV-2201, EV-2301), a first condenser (C- 7301) receiving vapours generated by the first stage evaporator (EV-2101) and a second condenser (C-7302) receiving vapours generated by the third stage evaporator (EV-2301), a vapour condensate tank (T-7301) collecting vapour condensate from the first (C-7301) and second (C-7302) condenser and condensed heating vapour from the first stage evaporator (EV-2101), a caustic exit line feeding molten 92 to 99 % caustic to a flaking unit comprising at least one flaking machine (F-3111, F-3112).

Single channel liquid membrane cell assemblies and cell bodies are disclosed. In some embodiments, the single channel liquid membrane cell assembly includes an elongate cell body having an elongate opening, a first bipolar plate adjacent the cell body, and a first gas diffusion electrode disposed between the cell body and the first bipolar plate. The first gas diffusion electrode spans across the entire length and width of the elongate opening of the cell body. The single channel liquid membrane cell assembly additionally includes a second bipolar plate adjacent the cell body such that the cell body is disposed between the first and second bipolar plates. The elongate opening horizontally defines an open area and the first gas diffusion electrode and the second bipolar plate vertically define the open area therebetween.

The application discloses a testing device and a testing method for carbon dioxide permeability of a permeation membrane. The testing device comprises a sample feeding system for feeding and processing a standard solution, a membrane cell system for installing a gas permeation membrane, and a measurement and circulation system for self-cleaning and measuring conductivity. The sample feeding system delivers a sodium carbonate standard solution through a syringe pump and injects phosphoric acid through an acid agent syringe pump to acidify the standard solution to release carbon dioxide. The membrane cell system clamps the membrane to be tested between an upstream chamber and a downstream chamber to simulate a liquid-liquid separation environment. The measurement and circulation system continuously monitors the conductivity change of the downstream solution through a sensor and combines a self-cleaning circuit to ensure measurement accuracy. The testing method indirectly calculates the carbon dioxide permeation flux and permeability based on the conductivity change. The application can realize high sensitivity, low cost and simple operation of the permeation membrane carbon dioxide permeability test in a liquid-liquid environment, and solves the problem of working condition simulation distortion of the existing gas-gas phase testing device.

The invention relates to the technical field of medical cosmetology, and discloses an elastic fat filling and shaping system which comprises a fat pretreatment module, an elastic supporting module, a dynamic injection module, a biological fixation module, a degradation regulation and control module and a postoperative shaping module. The fat pretreatment module comprises a multi-stage centrifugal separation unit, a cell activation unit and a viscoelasticity adjusting unit, the multi-stage centrifugal separation unit adopts a gradient centrifugal device and an fat cell screening membrane, and the cell activation unit is integrated with a low-temperature mixing cavity of fat cells and platelet-rich plasma (PRP); the viscoelasticity adjusting unit preloads temperature-sensitive sodium alginate gel through an injector, and the fat pretreatment module, the elastic supporting module, the dynamic injection module, the biological fixation module, the degradation regulation and control module and the postoperative shaping module are integrated, so that the elastic fat filling and shaping system is simpler and more convenient to operate and can better adapt to individual differences, and the stability of the elastic fat filling and shaping system is improved. The survival rate and the shaping effect of fat transplantation are improved.

The invention belongs to the technical field of preparation of antibacterial preparations, and particularly relates to an antibacterial composition, an antibacterial nano-emulsion, an antibacterial preparation and application of the antibacterial composition, the antibacterial nano-emulsion and the antibacterial preparation. Through verification, the benzalkonium bromide and the chlorhexidine acetate can achieve the effect of synergistically resisting methicillin-resistant staphylococcus aureus (MRSA) by inhibiting or removing a biological membrane (cell membrane), and particularly, the nano-emulsion prepared from the benzalkonium bromide and the chlorhexidine acetate has an excellent MRSA resisting effect; the minimum inhibitory concentration (MIC) of the prepared nanoemulsion on MRSA bacteria is 1.5625 [mu] g / mL, and the minimum bactericidal concentration (MBC) of the prepared nanoemulsion on MRSA bacteria is 6.25 [mu] g / mL; in addition, the prepared nanoemulsion is low in toxicity and excellent in stability. Moreover, the antibacterial preparation disclosed by the invention is simple in preparation process, stable in quality and suitable for large-scale production, can be widely applied to infection of multiple drug-resistant bacteria of skin wounds, and has a good market application prospect.

The utility model relates to the technical field of cell cultivators, and provides a bottle cap structure and a breathable membrane cell cultivator, the bottle cap structure at least comprises a cap body, the cap body is provided with an abdicating hole; the sealing adapter is connected with the cover body; the sealing adapter comprises an inner pipe joint and an outer pipe joint which are communicated with each other; the inner pipe connector is located on the inner side of the cover body and used for being connected with a pipeline located in the cover body. The outer pipe connector extends out of the cover body from the receding hole and is used for being connected with a pipeline located outside the cover body. According to the bottle cap structure provided by the utility model, compared with the structural design that a hole is formed in the cap and the pipeline directly penetrates out of the hole of the cap in the prior art, the inner pipeline and the outer pipeline are respectively and fixedly connected by utilizing the sealing adapter mounted on the cap body and the inner pipe joint and the outer pipe joint on the sealing adapter; therefore, the problem of poor sealing performance caused by direct connection of the pipeline and the inner wall of the hole is solved, and the pollution risk in the cell culture process is reduced.

The present invention belongs to the technical field of human bionic organ chips, and in particular relates to a microfluidic glomerular chip with intercellular signal transduction and filtration functions, comprising: a valve control layer, an endothelial cell-mesangial cell culture layer, a podocyte culture layer and a support layer arranged in sequence from top to bottom; the endothelial cell-mesangial cell culture layer comprises an endothelial cell culture combination chamber and a mesangial cell culture combination chamber provided on a second substrate, the endothelial cell culture combination chamber and the mesangial cell culture combination chamber being connected via a channel; the valve control layer is used to control the on-off of the channel; the podocyte culture layer comprises a podocyte culture-primary urine test combination chamber provided on a third substrate, the liquid inlet end of the podocyte culture-primary urine test combination chamber being connected to the endothelial cell culture combination chamber via a porous membrane; the liquid inlet end of the endothelial cell culture combination chamber, the liquid outlet end of the mesangial cell culture combination chamber and the liquid outlet end of the podocyte culture-primary urine test combination chamber extending upward to the valve control layer.