74 results about "Membrane cell" patented technology

The invention provides an organ chip unit for simulating the pathogenesis of rheumatoid arthritis, and belongs to the technical field of cell culture. The chip comprises a top-layer chip, a bottom-layer chip and a middle microporous membrane, the top-layer chip and the bottom-layer chip are separated by the middle microporous membrane, and the middle microporous membrane is used for enabling cells in a cell culture space of the top-layer chip and cells in a cell culture space of the bottom-layer chip not to be in direct contact, but interaction of signal molecules and substance exchange can be carried out; synovial cells and cartilage cells are injected into the bottom-layer chip or the top-layer chip to be cultured. According to the invention, multi-cell three-dimensional co-culture can be realized. Through the additional stress stimulation co-culture part, the influence of pressure or tension on the cell environment can be judged, and the actual condition that rheumatoid arthritis joints receive pressure or tension for a long time can be simulated. The synovial cells and the cartilage cells are used, so that the structure of the inflammation joint in a living body can be truly reflected, and the interaction among various cell tissues can be reflected.

The invention belongs to the technical field of cell culture, and discloses a multi-storage-module decidua uteri cell culture device which comprises a culture box body, a sealing cover plate is arranged at the upper end of the left side of the culture box body and rotationally connected with the culture box body, and a limiting sliding groove is formed in the inner wall of the culture box body; a placement supporting plate is arranged in the culture box body, limiting sliding blocks are connected to the two sides of the placement supporting plate, a placement groove is formed in the upper end of the placement supporting plate, a culture dish body is arranged in the placement groove, a guide groove is formed in the upper end of the left side of the culture box body, and a sealing plate is arranged in the guide groove. According to the decidua uteri cell culture device with the multiple storage modules, the sealing structure is arranged, the sealing cover plate is used for providing power, and when the sealing cover plate is opened, the sealing plate can be driven to move downwards to seal the taking opening, so that it is ensured that the internal temperature of the culture box body is not affected by the external environment to be changed; and stable growth of other cultured cells inside is ensured.

The invention discloses a PVDF recovery coupled wastewater treatment process. Waste liquid generated during PVDF recovery flows through a membrane pool and a treatment pool in sequence. A first driving pump drives a first ultrafine bubble generator to work. Ultrafine bubbles are generated at the outlet of the first ultrafine bubble generator. The ultrafine bubbles absorb PVDF particles in the waste liquid and float upward. The waste liquid in the membrane pool passes through a filtering flat membrane and enters the treatment pool. During the process of the waste liquid flowing through the treatment pool, a second driving pump arranged in the treatment pool drives a second ultrafine bubble generator to work. Ozone is introduced into an air inlet of the second ultrafine bubble generator. Ultrafine bubbles containing ozone are generated at the outlet of the second ultrafine bubble generator. The wastewater is treated by ozone and hydroxyl radicals generated by the collapse of the ultrafine bubbles.

The present invention relates to a method of producing cells having the phenotype of cardiac valve cells from precursor cells of valve cells comprising (a) introducing into said precursor cells early growth response 3 (EGR3) and / or a nucleic acid sequence encoding EGR3. The present invention also relates to a valve transplant comprising cells having the phenotype of valve cells obtained or obtainable by the method of the invention. The present invention furthermore relates to cells having the phenotype of valve cells obtained or obtainable by the method of the invention, the valve transplant of the invention or a compound promoting the expression and / or the activity of early growth response 3 (EGR3) for use in treating a subject having a valve defect.

Single channel liquid membrane cell assemblies and cell bodies are disclosed. In some embodiments, the single channel liquid membrane cell assembly includes an elongate cell body having an elongate opening, a first bipolar plate adjacent the cell body, and a first gas diffusion electrode disposed between the cell body and the first bipolar plate. The first gas diffusion electrode spans across the entire length and width of the elongate opening of the cell body. The single channel liquid membrane cell assembly additionally includes a second bipolar plate adjacent the cell body such that the cell body is disposed between the first and second bipolar plates. The elongate opening horizontally defines an open area and the first gas diffusion electrode and the second bipolar plate vertically define the open area therebetween.

The utility model discloses an air-cooled hydrogen fuel cell bipolar plate, a single cell and an electric pile, and belongs to the technical field of air-cooled hydrogen fuel cells. The bipolar plate comprises a plate body, a first groove is formed in one side face of the plate body, a cathode runner is arranged in the first groove, a second groove is formed in the other side face of the plate body, an anode runner is arranged in the second groove, and an air inlet and an air outlet are formed in the two ends of the plate body respectively. And the air inlet and the air outlet are respectively communicated with two ends of the anode runner. The cathode flow channel adopts a parallel flow channel; and the anode runner adopts a snakelike runner. The anode flow channel and the cathode flow channel of the bipolar plate are both arranged in the sunken open groove, and the compression amount of the gas diffusion layer is controlled according to the depth of the open groove; according to the structure, the problem that a gas diffusion layer in a traditional membrane battery is prone to overpressure can be effectively prevented, so that the diffusion rate and uniformity of hydrogen and oxygen between the membrane electrodes are improved, and the response speed and the output performance of the single-frame membrane electrode are further improved.

To provide a method for evaluating the quality of neuroretina for transplantation and neuroretina sheets for transplantation selected by said method. [Solution] The present invention provides a method for evaluating the quality of neuroretina for transplantation, comprising: extracting a part or all of a cell aggregate containing a neuroretina having an epithelial structure derived from pluripotent stem cells as a quality evaluation sample; detecting the expression of neuroretinal cell-related genes and non-neuroretinal cell-related genes in the quality evaluation sample; and determining, if the expression of neuroretinal cell-related genes is observed and the expression of non-neuroretinal cell-related genes is not observed, that (1) a neuroretina (neuroretina for transplantation) from the same cell aggregate as the cell aggregate containing a part of the quality evaluation sample, (2) a neuroretina (neuroretina for transplantation) from the same lot as the cell aggregate containing a part of the quality evaluation sample, or (3) a neuroretina (neuroretina for transplantation) from the same lot as the cell aggregate of the entire quality evaluation sample, wherein the non-neuroretinal cell-related genes include one or more genes selected from the group consisting of brain and spinal cord tissue marker genes and eyeball-related tissue marker genes.

The present invention provides: nanocomposites for temporary photoperforation of a cell membrane, formed by combining (i) gold nanoparticle and (ii) upconverting nanoparticles comprising ytterbium (Yb3+) and erbium (Er3+); and a pharmaceutical composition comprising same. The nanocomposite according to the present invention enables the use of near-infrared region light, which can effectively penetrate tissue, and enables temporary photoperforation through the formation of vapor nanobubbles caused by a momentary increase in temperature, and thus can promote the penetration of molecules (for example, drugs) into the plasma membrane / cell membrane.

This invention discloses a single-cell spatial annotation method that integrates image and sequencing data. It obtains candidate cue point locations, cell nucleus instance segmentation masks, and cell nucleus type probability vectors by processing full-field H&E stained tissue images. It also obtains a preprocessed spatial transcriptome raw sequencing matrix and corresponding cell type labels by processing the raw spatial transcriptome sequencing matrix. Each capture site and its corresponding integrated data structure are constructed. Based on rigid constraints on the number of cells allocated to each type within each capture site and main type quota constraints, the main type annotation is written. For cells in the intercapture zone, one or more neighboring capture sites with existing annotation results are found. The main type and cell subtype of the intercapture zone cells are inferred through morphological feature similarity calculation, thus obtaining the annotation result. This solves the problem that existing technologies cannot determine the exact type of each cell in ST-sequencing tissue.

The application discloses a kind of corneal near physiological biomechanics intelligent simulation method and device, it is related to biomechanics and biomechanics experimental device technical field, the present application includes mutually connected force load cavity, force loading component, sensor monitoring feedback component etc..The present application can realize to corneal repair material, corneal cell, corneal cell-material compound loading individual and compound flow shear force, static pressure, dynamic pressure, static tension and dynamic tension, gas-liquid dynamic culture is simultaneously carried out, and through pressure and flow sensor, the stress size of loading is monitored in real time, reach accurate intelligent comprehensive simulation cornea in body environment.The scheme provided by the present application can be used for the mechanical property detection of corneal tissue and material, obtains the material attribute under near physiological state;It can also be used for the mechanical loading of cell, to observe the cell behavior and functional change after near physiological mechanical loading.

The utility model belongs to the technical field of medical instruments, and particularly relates to a reusable heavy ion microporous membrane cell filtering device which comprises a lower cylinder component, comprising a lower cylinder main body, an extension part arranged at the top of the lower cylinder main body, an external thread arranged on the surface of the extension part, a mounting groove formed in the top of the extension part, and a heavy ion microporous membrane main body arranged in the mounting groove; the upper barrel assembly comprises an upper barrel main body arranged at the top of the lower barrel main body, an internal thread arranged at the bottom of the inner side wall of the upper barrel, and a limiting ring arranged on the inner side wall of the upper barrel; the structure is reasonable, in the using process, the heavy ion microporous membrane is convenient to replace, repeated use is convenient, the detection cost is reduced, and meanwhile generation of medical waste is reduced.

The utility model relates to the technical field of water treatment, in particular to a membrane pool water treatment system which comprises a membrane pool provided with a plurality of galleries, a medicament storage tank, a dosing pump, a dosing main pipeline, a plurality of branch pipelines and a controller, water inlet flow meters are arranged at the water inlet ends of the galleries; a first flow regulating valve is arranged on each branch pipeline; and the water inlet flow meter, the first flow regulating valve and the dosing pump are all in signal connection with the controller. According to the utility model, the defect in the prior art that the dosage cannot be respectively and accurately added manually according to the actual water quantity of each gallery is overcome, and the actual water quantity of the gallery can be measured by the water inlet flow meter, the medicament storage tank, the dosing pump, the first flow regulating valve and the controller; the controller can control the start and stop of the dosing pump and the opening degree of the first flow regulating valve according to the actual water quantity, so that the dosing amount of the medicament in each gallery is accurately controlled, the medicament is prevented from being excessive or insufficient, the flocculation effect can be ensured, the waste of the medicament can be avoided, and the medicament cost is saved.

The application discloses a dislocation tooth storage and treatment box and a storage and treatment method. The box comprises a box body and a box cover. One side of the box cover is hinged to the box body, and the other side of the box cover is detachably connected to the box body. The box body is internally provided with a cleaning groove, a liquid storage groove, a tooth storage groove and a storage groove. The cleaning groove and the tooth storage groove penetrate through the bottom of the box body, and a sealing plug is detachably connected to the bottom of the box body. The cleaning groove, the liquid storage groove and the tooth storage groove are respectively detachably connected with a cleaning groove cover, a liquid storage groove cover and a tooth storage groove cover, which are fixedly connected to the box cover. A pair of tweezers is detachably connected to the storage groove. The scheme uses physiological saline to flush dirt on the dislocation tooth, uses HBSS to maintain and recombine cell components consumed by periodontal membrane cells, provides a good storage environment, uses HBSS containing ALN to treat the dislocation tooth to reduce root absorption after replantation, avoid bone adhesion and improve the replanting success rate.

A liquid membrane cell assembly is disclosed. In some embodiments, the liquid membrane cell assembly includes an elongated base having opposing first and second ends and a central portion disposed therebetween. The first and second ends each include an elongated body, an electrolyte channel in the body, an electrolyte port fluidly connected to the electrolyte channel, a fuel channel in the body, and a fuel port fluidly connected to the fuel channel. The central portion includes spaced apart and opposing first and second members connecting the bases of the first and second portions and defining an open area therebetween in a horizontal direction. The liquid membrane cell assembly further includes an anode adjacent the first and second members and a cathode adjacent the first and second members such that the base is disposed between the anode and the cathode. The anode and cathode vertically define an open area therebetween.

The invention discloses a method for calculating a diffusion coefficient of micro-pollutants in a nanofiltration membrane based on adsorption capacity in the technical field of membranes, which comprises the following steps: installing the nanofiltration membrane in a membrane pool filled with a salt solution, dissolving the micro-pollutants in the salt solution, and intercepting and adsorbing the micro-pollutants by the nanofiltration membrane in the membrane pool; and soaking the nanofiltration membrane which adsorbs the micro-pollutants in an elution solution to desorb the micro-pollutants. According to the method, the adsorption capacity of the micropollutants in the nanofiltration membrane is obtained through conventional micropollutant interception, adsorption and desorption experiments, the diffusion coefficient can be calculated, the testing method is simple, the mutual relation between the micropollutants and the membrane and the mutual relation between the micropollutants and the solvent are considered in the mass transfer model calculation process, and the calculation accuracy is high. The influence of the complex interaction force of the membrane-micropollutants on the mass transfer process of the micropollutants is introduced, so that the calculation of the diffusion coefficient of the micropollutants except ions in the membrane is more accurate.

The invention belongs to the technical field of medicines, and particularly relates to application of a licorice extract in preparation of a medicine for treating premature ovarian failure. On the basis of network pharmacology prediction and animal experiment verification, through experimental exploration, it is clear that diammonium glycyrrhizinate can treat premature ovarian failure by improving ovarian tissue energy metabolism, reducing the oxidative stress level and inhibiting ovarian cell apoptosis, and also can treat premature ovarian failure by improving the ovarian growth and development microenvironment. According to the present invention, the growth and development rate of ovarian granular cells and membrane cells is improved, the sufficient quantity of granular cells and membrane cells are ensured to secrete female progestational hormone, the damaged ovarian tissue is further protected, the new strategy and hope are provided for the treatment of premature ovarian failure, and the pharmaceutical composition is expected to become the safe and effective treatment drug.

The utility model discloses a cell culture carrier capable of recycling cells, and relates to the technical field of exosome production and cell recycling, the cell culture carrier comprises a cell growth layer used for cell growth, the outer wall of the cell growth layer is provided with a thin film, the outer wall of the thin film is provided with a thick film, and the thick film is provided with a plurality of cells. According to the cell culture carrier capable of recycling the cells, after cell amplification reaches a plateau phase, 10%-90% of intra-membrane cells can dissociate and fall off from the side wall of a membrane by increasing the rotating speed without a digestion process, and the viability of the free cells is greater than 80%; the purpose of cell recovery can be achieved through operations such as centrifugation, and the cell amplification and recovery process is greatly simplified; the intra-membrane cells can be continuously amplified and harvested by continuously facilitating falling and updating, meanwhile, the yield per unit volume of the exosome after the cell growth layer is improved is also improved to a certain extent compared with the yield of the exosome of a common structure (two layers of membranes and one layer of spacing net) in the market, and the total yield can be improved through multi-batch harvesting.

1. The name of the design product: gas-liquid pipeline (gas permeable membrane cell culture device). 2. The use of the design product: gas-liquid pipeline for connecting gas permeable membrane cell culture device. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The invention belongs to the technical field of electrolytic synthesis, and particularly relates to a method for preparing formic acid through three-chamber two-membrane electrolysis of CO2. The method is based on a three-chamber two-membrane electrolysis device which is divided into an anode chamber, a middle chamber and a cathode chamber by a cationic membrane and a nanofiltration membrane, a concentrated KOH aqueous solution is added into the anode chamber, a dilute KOH solution is added into the middle chamber, and CO2 and water are introduced into the cathode chamber; and after a power supply is switched on for full reaction, dilute KOH solutions are obtained in the anode chamber and the intermediate chamber respectively, and a KCOOH product is obtained in the cathode chamber. According to the method, the utilization rate of CO2 is remarkably increased, the electrolysis power consumption is reduced, the problems of water logging and the like are avoided, and the operation life is greatly prolonged.

An object of the present invention is to provide a cultured cell sheet that can solve problems accompanied by defects of mucosal tissue, such as inflammation in mucosal tissues covering bone tissues and granulation tissue formation. The present inventors have found that a cultured cell sheet obtained by culturing cells collected from a nasal mucosal tissue as a source under specific conditions, and reconstructing the cells into a sheet are engrafted onto mucosal tissue defective sites; promote the regeneration of mucosal tissues; efficiently reduce inflammation that occurs in a middle ear bone tissue; and prevent fibrosis and the like that are characteristic of bone tissue inflammation, thereby demonstrating that the cultured cell sheet can solve the problems. It is also found that the cultured cell sheet of the present invention is also useful for preventing inflammation of bone tissues in areas other than the middle ear.

The utility model provides a tangential flow membrane pool structure which comprises a membrane pool assembly, the membrane pool assembly is located on a pressing assembly, a press machine is installed at the bottom of the pressing assembly, and the membrane pool assembly comprises an upper-layer membrane pool structure, a middle-layer membrane pool structure, a lower-layer membrane pool structure, a plurality of filtering membranes and a plurality of membrane supporting pieces. The tangential flow membrane pool structure has the beneficial effects that the tangential flow membrane pool structure is a layered structure, the layered structure is detachable, and the sectional area of a single layer is larger; multiple layers can be stacked for use; meanwhile, the high sealing performance and the high pressure resistance are achieved through the pressing assembly; and the pressing assembly is also convenient to operate.

The invention belongs to the technical field of sewage treatment, and particularly relates to an oxygen-eliminating low-energy-consumption sewage treatment system which comprises an AOA reaction tank, an MBR membrane tank, a separation unit, an oxygen-eliminating unit, a first reflux unit and a second reflux unit, sludge precipitates separated by the separation unit are conveyed into the oxygen elimination unit, and supernatant liquid is conveyed into an aerobic zone of the AOA reaction tank through a first reflux unit; the second reflux unit is used for conveying the sludge in the oxygen elimination unit into the anaerobic zone and / or the anoxic zone of the AOA reaction tank. Dissolved oxygen in the separation unit is fully utilized, meanwhile, the sludge backflow concentration is improved, and under the condition that the sludge concentration of the AOA reaction tank is ensured, the backflow amount of backflow liquid is reduced as much as possible; according to the present invention, the reflux energy consumption can be reduced, and the oxygen in the reflux sludge is completely eliminated after the reflux sludge is subjected to the oxygen elimination treatment, such that the maintenance and the guarantee of the anoxic environment, the anaerobic environment and the aerobic environment in the AOA reaction tank are easily achieved, the denitrification and phosphorus removal effects are improved, and the energy consumption is reduced.

The invention belongs to the technical field of proton exchange membrane cells, and discloses a cathode graphite polar plate for a fuel cell, which comprises a public channel, a gas distribution area and an active area, a hydrogen inlet public channel, a cooling water inlet public channel, an air inlet public channel, a hydrogen outlet public channel, a cooling water outlet public channel, an air outlet public channel, an air inlet distribution port, an air outlet distribution port, a positioning hole, a gas distribution area and a three-dimensional channel with a contraction and expansion structure are arranged on the cathode graphite polar plate. The cathode graphite polar plate channel has the characteristic of combining a three-dimensional waveform structure and a shrinkage-expansion structure. The runners on the cathode graphite polar plate are designed in a staggered manner. And the spacing, the arrangement mode and the flow guide angle of the flow guide plates are optimized in the distribution area, so that gas is uniformly distributed to each channel of the bipolar plate, and the performance and the efficiency of the fuel stack are favorably improved. Cathode graphite polar plate channels are all formed by carving, so that the processing is convenient, and the batch production is facilitated.

Liquid membrane cell assemblies are disclosed. In some embodiments, the liquid membrane cell assembly includes an elongate base having opposed first and second end portions and a central portion disposed therebetween. The first and second end portions each includes an elongate body, an electrolyte channel within the body, an electrolyte port fluidly connected to the electrolyte channel, a fuel channel within the body, and a fuel port fluidly connected to the fuel channel. The central portion includes spaced and opposed first and second members that connect the bases of the first and second portions and that horizontally define an open area therebetween. The liquid membrane cell assembly additionally includes an anode adjacent the first and second members, and a cathode adjacent the first and second members such that the base is disposed between the anode and the cathode. The anode and cathode vertically define the open area therebetween.

The invention relates to a preparation method of high-purity methanol, and belongs to the technical field of methanol generation, the preparation method of the high-purity methanol comprises the following operation steps: placing a modified polyethersulfone microfiltration membrane in a membrane pool, introducing crude methanol into the membrane pool, and filtering to obtain the high-purity methanol, the flow velocity of the crude methanol in the membrane pool purification process being 1-3 L / min, and the permeation side pressure being 10-60 kPa; the preparation method of the modified polyethersulfone microporous filter membrane comprises the following steps: preparing a membrane by using a phase inversion method, adding Zn-MOF-74 into DMAc, adding polyethersulfone after complete dispersion to obtain a membrane casting solution, casting the prepared membrane casting solution on a smooth glass plate, and performing vacuum drying to obtain the modified polyethersulfone microporous filter membrane. The membrane separation technology is adopted, the methanol separation and purification efficiency is effectively improved on the premise that equipment investment is not increased and purification energy consumption is saved, and the content of the prepared methanol can reach 99.99% or above.

The utility model relates to the technical field of cell culture devices, and provides a gas-permeable membrane cell culture bottle, which at least comprises a bottle bottom provided with a first clamping body; the bottle bottom fixing frame is arranged outside the bottle bottom in a sleeving mode, a second clamping body matched with the first clamping body is arranged on the bottle bottom fixing frame, and during assembling, the first clamping body and the second clamping body are clamped with each other so that the bottle bottom can be prevented from falling off from the bottle bottom fixing frame. According to the breathable membrane cell culture bottle, the first clamping body is designed at the bottle bottom, the second clamping body is arranged at the corresponding position of the bottle bottom fixing frame, and during assembly, the first clamping body and the second clamping body are clamped with each other, so that stable connection between the bottle bottom and the bottle bottom fixing frame is ensured, and the bottle bottom is prevented from falling off from the bottle bottom fixing frame.

The invention is applicable to the technical field of cell models, and provides a method for establishing a porcine nasal mucosa M cell differentiation model, which comprises the following steps: preparing porcine nasal mucosa epithelial cells; construction of a pGEX-6p-1-RANKL recombinant expression vector, and prokaryotic expression of the recombinant expression vector; inducing the porcine nasal mucosa epithelial cells to differentiate into M cells by the porcine RANKL; and RT-qPCR (real-time quantitative polymerase chain reaction) is used for detecting the expression conditions of M cell differentiation related genes Spi-B, Tnfaip2 and GP2. According to the invention, the functional pig source RANKL recombinant protein is successfully constructed, and pig nasal mucosa epithelial cells are successfully induced to be differentiated into M cells by using the functional pig source RANKL recombinant protein. Through an RT-qPCR experiment, the expression level of M cell related genes is accurately detected, and the differentiation efficiency is verified. The achievement not only provides a tool for research on porcine respiratory diseases and development of drug vaccines, but also promotes research on cross-species mucosal immunity, and provides a new perspective and an experimental tool for the field of mucosal immunity.

The present invention provides a pharmaceutical composition containing a VEGF nanobody and its use. Specifically, the present invention provides a pharmaceutical composition containing a VEGF nanobody, a cell-penetrating peptide and a pharmaceutically acceptable carrier. The pharmaceutical composition of the present invention can effectively penetrate the cell membrane, and the cell-penetrating peptide contained therein can significantly promote the cell membrane permeability of the VEGF nanobody. The pharmaceutical composition of the present invention can effectively penetrate cancer cells and corneal cells. With the increase of the concentration of the cell-penetrating peptide, the uptake effect of the VEGF nanobody on the cell is significantly improved, and at high doses, it has only slight toxicity and has very good safety.

The invention relates to the technical field of biomedicine, in particular to a method for promoting formation of nerve fasciae and repair and regeneration after sciatic nerve defect by using small extracellular vesicles (hfNCSCs-sEVs) derived from hair follicle neural crest stem cells and application. According to the application disclosed by the invention, the effects of the hfNCSCs-sEVs on proliferation, migration, tube formation and barrier formation of nerve fascia cells in vitro are defined; the effect of hfNCSCs-sEVs on tissue regeneration and function recovery after rat sciatic nerve defect is defined, the hfNCSCs-sEVs inhibits expression of Smad7 in nerve tract membrane cells by transferring miR-21-5p, activates a TGF-beta / Smad signal channel, up-regulates expression of HAS2, promotes proliferation and migration of HAS2 and promotes expression of tight junction protein thereafter, and the hfNCSCs-sEVs can be used for treating rat sciatic nerve defect. And a new view angle and theoretical support are provided for repair and regeneration of sciatic nerve injury.

The application discloses a kind of electroactive microorganism detection method and kit.The application utilizes the catalytic action of electroactive microorganism extracellular electron transfer key protein (outer membrane cytochrome c) and chemiluminescence technology, combined with the photographing function of smart phone, picture information processing technology, provide a kind of electroactive microorganism detection method and detection kit.No need to use any nanomaterial or special equipment, by commonly used smart phone and computer application program can realize signal acquisition, processing, calculation and result output;Convenient and fast, good stability, low cost, simple operation, the whole detection process can be completed without professional training;And response speed is fast, the whole detection process can be completed in 2min;It can be widely used in field detection.