28 results about "Membrane cell" patented technology

Single channel liquid membrane cell assemblies and cell bodies are disclosed. In some embodiments, the single channel liquid membrane cell assembly includes an elongate cell body having an elongate opening, a first bipolar plate adjacent the cell body, and a first gas diffusion electrode disposed between the cell body and the first bipolar plate. The first gas diffusion electrode spans across the entire length and width of the elongate opening of the cell body. The single channel liquid membrane cell assembly additionally includes a second bipolar plate adjacent the cell body such that the cell body is disposed between the first and second bipolar plates. The elongate opening horizontally defines an open area and the first gas diffusion electrode and the second bipolar plate vertically define the open area therebetween.

The utility model discloses an air-cooled hydrogen fuel cell bipolar plate, a single cell and an electric pile, and belongs to the technical field of air-cooled hydrogen fuel cells. The bipolar plate comprises a plate body, a first groove is formed in one side face of the plate body, a cathode runner is arranged in the first groove, a second groove is formed in the other side face of the plate body, an anode runner is arranged in the second groove, and an air inlet and an air outlet are formed in the two ends of the plate body respectively. And the air inlet and the air outlet are respectively communicated with two ends of the anode runner. The cathode flow channel adopts a parallel flow channel; and the anode runner adopts a snakelike runner. The anode flow channel and the cathode flow channel of the bipolar plate are both arranged in the sunken open groove, and the compression amount of the gas diffusion layer is controlled according to the depth of the open groove; according to the structure, the problem that a gas diffusion layer in a traditional membrane battery is prone to overpressure can be effectively prevented, so that the diffusion rate and uniformity of hydrogen and oxygen between the membrane electrodes are improved, and the response speed and the output performance of the single-frame membrane electrode are further improved.

To provide a method for evaluating the quality of neuroretina for transplantation and neuroretina sheets for transplantation selected by said method. [Solution] The present invention provides a method for evaluating the quality of neuroretina for transplantation, comprising: extracting a part or all of a cell aggregate containing a neuroretina having an epithelial structure derived from pluripotent stem cells as a quality evaluation sample; detecting the expression of neuroretinal cell-related genes and non-neuroretinal cell-related genes in the quality evaluation sample; and determining, if the expression of neuroretinal cell-related genes is observed and the expression of non-neuroretinal cell-related genes is not observed, that (1) a neuroretina (neuroretina for transplantation) from the same cell aggregate as the cell aggregate containing a part of the quality evaluation sample, (2) a neuroretina (neuroretina for transplantation) from the same lot as the cell aggregate containing a part of the quality evaluation sample, or (3) a neuroretina (neuroretina for transplantation) from the same lot as the cell aggregate of the entire quality evaluation sample, wherein the non-neuroretinal cell-related genes include one or more genes selected from the group consisting of brain and spinal cord tissue marker genes and eyeball-related tissue marker genes.

This invention discloses a single-cell spatial annotation method that integrates image and sequencing data. It obtains candidate cue point locations, cell nucleus instance segmentation masks, and cell nucleus type probability vectors by processing full-field H&E stained tissue images. It also obtains a preprocessed spatial transcriptome raw sequencing matrix and corresponding cell type labels by processing the raw spatial transcriptome sequencing matrix. Each capture site and its corresponding integrated data structure are constructed. Based on rigid constraints on the number of cells allocated to each type within each capture site and main type quota constraints, the main type annotation is written. For cells in the intercapture zone, one or more neighboring capture sites with existing annotation results are found. The main type and cell subtype of the intercapture zone cells are inferred through morphological feature similarity calculation, thus obtaining the annotation result. This solves the problem that existing technologies cannot determine the exact type of each cell in ST-sequencing tissue.

The utility model relates to the technical field of water treatment, in particular to a membrane pool water treatment system which comprises a membrane pool provided with a plurality of galleries, a medicament storage tank, a dosing pump, a dosing main pipeline, a plurality of branch pipelines and a controller, water inlet flow meters are arranged at the water inlet ends of the galleries; a first flow regulating valve is arranged on each branch pipeline; and the water inlet flow meter, the first flow regulating valve and the dosing pump are all in signal connection with the controller. According to the utility model, the defect in the prior art that the dosage cannot be respectively and accurately added manually according to the actual water quantity of each gallery is overcome, and the actual water quantity of the gallery can be measured by the water inlet flow meter, the medicament storage tank, the dosing pump, the first flow regulating valve and the controller; the controller can control the start and stop of the dosing pump and the opening degree of the first flow regulating valve according to the actual water quantity, so that the dosing amount of the medicament in each gallery is accurately controlled, the medicament is prevented from being excessive or insufficient, the flocculation effect can be ensured, the waste of the medicament can be avoided, and the medicament cost is saved.

The application discloses a dislocation tooth storage and treatment box and a storage and treatment method. The box comprises a box body and a box cover. One side of the box cover is hinged to the box body, and the other side of the box cover is detachably connected to the box body. The box body is internally provided with a cleaning groove, a liquid storage groove, a tooth storage groove and a storage groove. The cleaning groove and the tooth storage groove penetrate through the bottom of the box body, and a sealing plug is detachably connected to the bottom of the box body. The cleaning groove, the liquid storage groove and the tooth storage groove are respectively detachably connected with a cleaning groove cover, a liquid storage groove cover and a tooth storage groove cover, which are fixedly connected to the box cover. A pair of tweezers is detachably connected to the storage groove. The scheme uses physiological saline to flush dirt on the dislocation tooth, uses HBSS to maintain and recombine cell components consumed by periodontal membrane cells, provides a good storage environment, uses HBSS containing ALN to treat the dislocation tooth to reduce root absorption after replantation, avoid bone adhesion and improve the replanting success rate.

The invention discloses a method for calculating a diffusion coefficient of micro-pollutants in a nanofiltration membrane based on adsorption capacity in the technical field of membranes, which comprises the following steps: installing the nanofiltration membrane in a membrane pool filled with a salt solution, dissolving the micro-pollutants in the salt solution, and intercepting and adsorbing the micro-pollutants by the nanofiltration membrane in the membrane pool; and soaking the nanofiltration membrane which adsorbs the micro-pollutants in an elution solution to desorb the micro-pollutants. According to the method, the adsorption capacity of the micropollutants in the nanofiltration membrane is obtained through conventional micropollutant interception, adsorption and desorption experiments, the diffusion coefficient can be calculated, the testing method is simple, the mutual relation between the micropollutants and the membrane and the mutual relation between the micropollutants and the solvent are considered in the mass transfer model calculation process, and the calculation accuracy is high. The influence of the complex interaction force of the membrane-micropollutants on the mass transfer process of the micropollutants is introduced, so that the calculation of the diffusion coefficient of the micropollutants except ions in the membrane is more accurate.

The utility model discloses a cell culture carrier capable of recycling cells, and relates to the technical field of exosome production and cell recycling, the cell culture carrier comprises a cell growth layer used for cell growth, the outer wall of the cell growth layer is provided with a thin film, the outer wall of the thin film is provided with a thick film, and the thick film is provided with a plurality of cells. According to the cell culture carrier capable of recycling the cells, after cell amplification reaches a plateau phase, 10%-90% of intra-membrane cells can dissociate and fall off from the side wall of a membrane by increasing the rotating speed without a digestion process, and the viability of the free cells is greater than 80%; the purpose of cell recovery can be achieved through operations such as centrifugation, and the cell amplification and recovery process is greatly simplified; the intra-membrane cells can be continuously amplified and harvested by continuously facilitating falling and updating, meanwhile, the yield per unit volume of the exosome after the cell growth layer is improved is also improved to a certain extent compared with the yield of the exosome of a common structure (two layers of membranes and one layer of spacing net) in the market, and the total yield can be improved through multi-batch harvesting.

1. The name of the design product: gas-liquid pipeline (gas permeable membrane cell culture device). 2. The use of the design product: gas-liquid pipeline for connecting gas permeable membrane cell culture device. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The invention belongs to the technical field of electrolytic synthesis, and particularly relates to a method for preparing formic acid through three-chamber two-membrane electrolysis of CO2. The method is based on a three-chamber two-membrane electrolysis device which is divided into an anode chamber, a middle chamber and a cathode chamber by a cationic membrane and a nanofiltration membrane, a concentrated KOH aqueous solution is added into the anode chamber, a dilute KOH solution is added into the middle chamber, and CO2 and water are introduced into the cathode chamber; and after a power supply is switched on for full reaction, dilute KOH solutions are obtained in the anode chamber and the intermediate chamber respectively, and a KCOOH product is obtained in the cathode chamber. According to the method, the utilization rate of CO2 is remarkably increased, the electrolysis power consumption is reduced, the problems of water logging and the like are avoided, and the operation life is greatly prolonged.

The utility model provides a tangential flow membrane pool structure which comprises a membrane pool assembly, the membrane pool assembly is located on a pressing assembly, a press machine is installed at the bottom of the pressing assembly, and the membrane pool assembly comprises an upper-layer membrane pool structure, a middle-layer membrane pool structure, a lower-layer membrane pool structure, a plurality of filtering membranes and a plurality of membrane supporting pieces. The tangential flow membrane pool structure has the beneficial effects that the tangential flow membrane pool structure is a layered structure, the layered structure is detachable, and the sectional area of a single layer is larger; multiple layers can be stacked for use; meanwhile, the high sealing performance and the high pressure resistance are achieved through the pressing assembly; and the pressing assembly is also convenient to operate.

Liquid membrane cell assemblies are disclosed. In some embodiments, the liquid membrane cell assembly includes an elongate base having opposed first and second end portions and a central portion disposed therebetween. The first and second end portions each includes an elongate body, an electrolyte channel within the body, an electrolyte port fluidly connected to the electrolyte channel, a fuel channel within the body, and a fuel port fluidly connected to the fuel channel. The central portion includes spaced and opposed first and second members that connect the bases of the first and second portions and that horizontally define an open area therebetween. The liquid membrane cell assembly additionally includes an anode adjacent the first and second members, and a cathode adjacent the first and second members such that the base is disposed between the anode and the cathode. The anode and cathode vertically define the open area therebetween.

The utility model relates to the technical field of cell culture devices, and provides a gas-permeable membrane cell culture bottle, which at least comprises a bottle bottom provided with a first clamping body; the bottle bottom fixing frame is arranged outside the bottle bottom in a sleeving mode, a second clamping body matched with the first clamping body is arranged on the bottle bottom fixing frame, and during assembling, the first clamping body and the second clamping body are clamped with each other so that the bottle bottom can be prevented from falling off from the bottle bottom fixing frame. According to the breathable membrane cell culture bottle, the first clamping body is designed at the bottle bottom, the second clamping body is arranged at the corresponding position of the bottle bottom fixing frame, and during assembly, the first clamping body and the second clamping body are clamped with each other, so that stable connection between the bottle bottom and the bottle bottom fixing frame is ensured, and the bottle bottom is prevented from falling off from the bottle bottom fixing frame.

The application discloses a kind of electroactive microorganism detection method and kit.The application utilizes the catalytic action of electroactive microorganism extracellular electron transfer key protein (outer membrane cytochrome c) and chemiluminescence technology, combined with the photographing function of smart phone, picture information processing technology, provide a kind of electroactive microorganism detection method and detection kit.No need to use any nanomaterial or special equipment, by commonly used smart phone and computer application program can realize signal acquisition, processing, calculation and result output;Convenient and fast, good stability, low cost, simple operation, the whole detection process can be completed without professional training;And response speed is fast, the whole detection process can be completed in 2min;It can be widely used in field detection.

Single channel liquid membrane cell assemblies and cell bodies are disclosed. In some embodiments, the single channel liquid membrane cell assembly includes an elongate cell body having an elongate opening, a first bipolar plate adjacent the cell body, and a first gas diffusion electrode disposed between the cell body and the first bipolar plate. The first gas diffusion electrode spans across the entire length and width of the elongate opening of the cell body. The single channel liquid membrane cell assembly additionally includes a second bipolar plate adjacent the cell body such that the cell body is disposed between the first and second bipolar plates. The elongate opening horizontally defines an open area and the first gas diffusion electrode and the second bipolar plate vertically define the open area therebetween.

The application discloses a testing device and a testing method for carbon dioxide permeability of a permeation membrane. The testing device comprises a sample feeding system for feeding and processing a standard solution, a membrane cell system for installing a gas permeation membrane, and a measurement and circulation system for self-cleaning and measuring conductivity. The sample feeding system delivers a sodium carbonate standard solution through a syringe pump and injects phosphoric acid through an acid agent syringe pump to acidify the standard solution to release carbon dioxide. The membrane cell system clamps the membrane to be tested between an upstream chamber and a downstream chamber to simulate a liquid-liquid separation environment. The measurement and circulation system continuously monitors the conductivity change of the downstream solution through a sensor and combines a self-cleaning circuit to ensure measurement accuracy. The testing method indirectly calculates the carbon dioxide permeation flux and permeability based on the conductivity change. The application can realize high sensitivity, low cost and simple operation of the permeation membrane carbon dioxide permeability test in a liquid-liquid environment, and solves the problem of working condition simulation distortion of the existing gas-gas phase testing device.

The utility model relates to the technical field of cell cultivators, and provides a bottle cap structure and a breathable membrane cell cultivator, the bottle cap structure at least comprises a cap body, the cap body is provided with an abdicating hole; the sealing adapter is connected with the cover body; the sealing adapter comprises an inner pipe joint and an outer pipe joint which are communicated with each other; the inner pipe connector is located on the inner side of the cover body and used for being connected with a pipeline located in the cover body. The outer pipe connector extends out of the cover body from the receding hole and is used for being connected with a pipeline located outside the cover body. According to the bottle cap structure provided by the utility model, compared with the structural design that a hole is formed in the cap and the pipeline directly penetrates out of the hole of the cap in the prior art, the inner pipeline and the outer pipeline are respectively and fixedly connected by utilizing the sealing adapter mounted on the cap body and the inner pipe joint and the outer pipe joint on the sealing adapter; therefore, the problem of poor sealing performance caused by direct connection of the pipeline and the inner wall of the hole is solved, and the pollution risk in the cell culture process is reduced.

A co-culture device (100) includes a container body (110) in which a culture medium can be stored, and a cell culture insert (120). The cell culture insert (120) comprises a separation container main body (110) and the cell culture insert (120) and has an oxygen-permeable membrane (122). The cell culture method comprises: (a) a step for culturing cells inside a cell culture insert (120) so as to cover a film (122); (b) a step for introducing a first culture medium (161) exhibiting a first color into the container body (110); (c) a step of introducing a second culture medium (162) containing bacteria and exhibiting a second color different from the first color into the cell culture insert (120); and (d) a step of co-culturing the cells and the bacteria in a state in which the membrane (122) is disposed in contact with the first medium.

The present invention relates to a corneal cell culture support comprising graphene and its uses. The graphene-containing support of the present invention is non-toxic to corneal cells, is optimized for the cell proliferation rate, adhesion rate, and survival rate of corneal cells, enhances the expression of corneal cell-specific genes, and can maintain an optimized corneal cell state through cytoskeletal remodeling and mitochondrial activation. Therefore, the corneal cell culture support has the effect of being utilized in cell therapies, implant materials in the field of regenerative medicine, and tissue engineering platforms.

Provided herein are adeno-associated virus (AAV) particles comprising viral capsid proteins that preferentially transduce retinal cells. A modified AAV capsid protein disclosed herein comprises a framework protein and a peptide that is inserted into the framework protein. Also provided are nucleic acids encoding said modified AAV capsid protein. Also provided are methods of using the AAV particles, for example, for enhancing expression in retinal cells and for treating retinal disease.

The invention relates to a silver-plated diffusion plate with an innovative pore geometry produced by selective laser melting method for use as a liquid / gas permeable diffusion plate in Proton Exchange Membrane cells for the production of energy and high purity hydrogen in fuel cells and electrolyzers.

Electroactive tunable metamaterial structures, devices, and methods are disclosed. In some aspects, an acoustic metamaterial structure for acoustic imaging includes a plurality of piezoactive membrane cells, each including a first passivation layer; an electrode layer in contact with the first passivation layer; a piezoelectric layer in contact with the electrode layer; a bulk membrane layer in contact with the piezoelectric layer; and a second passivation layer in contact with the bulk membrane layer, wherein the acoustic metamaterial structure is configured to steer and / or focus propagation of an acoustic signal based on electrical control signals to the piezoactive membrane cells to affect an acoustic propagation property.

The application provides a plate type membrane differential pressure method gas permeability test device and process, the test device comprises a membrane cell, a vacuum control system, a computer control system, a raw material gas inlet system on the membrane, a purge gas inlet system under the membrane, a temperature control system, a flow and composition test system. The plate type membrane differential pressure method gas permeability test device provided by the application has high integration and environmental control function, and can be used for in-situ measurement under constant temperature or variable temperature conditions. After the plate type membrane is placed in the membrane cell and sealed through a gasket and a pressing plate in the test process, the constant pressure variable volume method or the constant volume variable pressure method can be used for plate type membrane material permeability test, so that the permeability of the plate type membrane and the intrinsic gas permeability of the material of the plate type membrane are obtained. Similarly, according to the structure characteristics of the plate type membrane, the device can be used for measuring the gas permeability and air permeability of various gas separation membranes, packaging materials and clothing fabrics.

The utility model relates to the technical field of electrodialysis, and provides a novel rack for an electrodialysis membrane module, which comprises a first clamping plate, a second clamping plate, a connecting underframe, two first cross rods, two second cross rods, a hydraulic pump, a first hydraulic jack and a second hydraulic jack, the second clamping plate is arranged on the two first transverse rods and the two second transverse rods in a sleeving mode in the mode that the second clamping plate can move towards the first clamping plate through an installation sleeve, and the clamping face of the second clamping plate faces the clamping face of the first clamping plate. The hydraulic pump is respectively connected with the first hydraulic jack and the second hydraulic jack through oil pipes to drive the first hydraulic jack and the second hydraulic jack to synchronously push the second clamping plate to move and apply pressure to the first clamping plate, so that the second clamping plate and the first clamping plate are matched to clamp the electrodialysis membrane module placed on the connecting underframe. The utility model solves the problem that the upper part or the lower part of the whole membrane module device is easy to have internal seepage and external leakage due to the fact that the existing rack for the electrodialysis membrane module device only applies pressure to the middle part of the clamping plate.

The invention provides a method for measuring the positive rate of cells expressing exogenous therapeutic genes by combining RNA (Ribonucleic Acid) hybridization and flow cytometry. The detection method comprises the following steps: designing a specific in-situ hybridization probe aiming at mRNA (Messenger Ribonucleic Acid) expressed by a target gene, carrying out RNA probe in-situ hybridization staining in the cells, and distinguishing specific cell subgroups by combining the single cell resolution of flow cytometry. A feasible detection method is provided for highly personalized and multivalent TCR and other cell treatment samples which do not have specific antibodies or cannot detect the positive rate by using a unique antibody, and the design space for designing the specific target RNA probe is larger and the difficulty is lower than that for designing the specific antibody. Comprising the following steps: fixing cell morphology and RNA, permeabilizing / breaking a cell membrane, fixing / perforating a cell nucleus, digesting intracellular and intracellular free single-stranded DNA, carrying out RNA probe hybridization and signal amplification, and carrying out flow detection.

The invention discloses a rapid detection kit for circadian rhythm imbalance of zebra fish cells. The rapid detection kit comprises a juvenile zebra fish splitting / RNA (Ribonucleic Acid) binding plate, a sealing plate membrane, a cell splitting solution, washing buffer solutions I and II, a DNase I digestive solution, a reaction buffer solution, a nuclease-free eluent, a 5x rapid reverse transcription premixed solution, a 2x SYBR Green qPCR (Polymerase Chain Reaction) premixed solution, a set of specific primer premixed solution and nuclease-free water. The invention also discloses an application of the kit. The invention also discloses a method for screening a compound or substance for regulating and controlling diurnal rhythm imbalance of zebrafish cells by using the kit. The invention can quickly, accurately and conveniently detect the expression of a plurality of core clock genes, is suitable for a kit in a high-throughput screening mode, and has urgent practical significance on environmental pollutant screening, clock regulation drug discovery and basic rhythm research.

1. The name of the design product: breathable membrane cell culture device. 2. The use of the design product: cell culture vessel. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: design 1 perspective view 1. 5. Design 1 is designated as the basic design.

The invention provides a sewage treatment low-cost centralized detection device with multi-point polling and membrane method pretreatment, comprising: a multi-point sampling system for automatically collecting water samples from a plurality of monitoring points of a sewage treatment process, the multi-point sampling system comprising electric valves and sampling pumps arranged at sampling points; the central detection unit is connected with the multi-point sampling system through a pipeline, and the central detection unit comprises a first flow cell and a second flow cell which are communicated in sequence; the membrane pretreatment unit is arranged between the first flow cell and the second flow cell and is used for filtering the water sample from the first flow cell to generate clear liquid to be supplied to the second flow cell, and the membrane pretreatment unit comprises an MBR (Membrane Bioreactor) membrane cell and a self-sucking pump for sucking and filtering. The invention provides a low-cost centralized detection device for sewage treatment based on multi-point polling and membrane pretreatment, and aims to solve the problems of high detection cost, poor data representativeness and real-time performance, insufficient coordination with a control system and the like in the prior art.