Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of nanogold-carbon nano tube-chitosan composite membrane cell sensor to detection of toxicity of food-borne pathogenic bacteria

A technology of cell sensors and carbon nanotubes, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of long inspection time, cumbersome operation, and high cost, so as to improve adhesion and vitality, improve sensitivity, and speed up detection The effect of speed

Active Publication Date: 2016-03-23
JIANGNAN UNIV
View PDF3 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to the cumbersome operation, these detection methods take a long time, are expensive, and have large operational errors.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of nanogold-carbon nano tube-chitosan composite membrane cell sensor to detection of toxicity of food-borne pathogenic bacteria
  • Application of nanogold-carbon nano tube-chitosan composite membrane cell sensor to detection of toxicity of food-borne pathogenic bacteria
  • Application of nanogold-carbon nano tube-chitosan composite membrane cell sensor to detection of toxicity of food-borne pathogenic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Preparation of electrochemical cell sensor

[0032] The construction process of electrochemical cell sensor is as follows: figure 1 As shown, first add HAuCl to the dispersion of carboxylated carbon nanotubes 4 Prepare a gold nano / carbon nanotube / chitosan dispersion, add it dropwise to the electrode to modify the electrode, and then endocytose the prepared magnetic nanoparticles into mouse macrophages, and use magnetism to fix the cells to the modified magnetic glass On the surface of the carbon electrode, a cell sensor is prepared.

[0033] 1. Nano-gold-carbon nanotube-chitosan composite film modified magnetic electrode

[0034] The cleaning method of the magnetic electrode magnetic glassy carbon electrode is as follows: After soaking the magnetic electrode (Φ=5mm) in the Piranha solution for 15 minutes, use 0.3 μm and 0.05 μm Al 2 o 3 Polish the powder, polish and polish the electrode surface into a mirror surface, then ultrasonically clean it with 1:...

Embodiment 2

[0041] Embodiment 2 Application of electrochemical cell sensor

[0042] 1. Detection of LPS

[0043] Weigh 1mgLPS and dissolve it in PBS to 1mgmL -1 solution. Diluted with RPMI1640 culture medium to concentrations of 1, 1.5, 2, 2.5, 3, 5 μg mL -1 of LPS solution. The modified cell sensing electrodes were placed in different concentrations of LPS solutions and incubated at 37°C for 3 hours, rinsed with PBS, and then inserted into the electrolytic cell for measurement.

[0044] 2. Analytical conditions and methods

[0045] The analysis method is as follows: the electrochemical workstation ATUOLAB under the conditions of the initial amplitude of 0.05V, the frequency of 1 ~ 100kHz, the reaction medium liquid is 2.5mMFe(CN) 6 3- / 4- Measured in the solution, using EIS method, and calculated by the best equivalent circuit, the impedance value of the modified working electrode adsorbed magnetic cells was used as the blank AC impedance value R et (Ab), the measured impedance val...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of a nanogold-carbon nano tube-chitosan composite membrane cell sensor to detection of toxicity of food-borne pathogenic bacteria and belongs to the technical field of detection of toxicity of Gram-negative pathogenic bacteria. According to the application, a magnetic glassy carbon electrode is modified with nanogold-carbon nano tube-chitosan with a self-assembly method, mouse macrophages Ana-1 endocytosing magnetic nanoparticles are taken as a sensing medium and directly adsorbed by the modified electrode, and the sensor is constructed; finally, the sensor is applied to lipopolysaccharide toxicity evaluation of the Gram-negative bacteria, has the obvious toxic effect change with the lipopolysaccharide concentration in the concentration range of 1-5 mu g mL<-1>, and has the detection limit of 0.3 mu g mL<-1>. The toxicity of the Gram-negative bacteria is detected with the method efficiently, conveniently and quickly, defects of traditional toxicity detection methods of the pathogenic bacteria are overcome, and the obtained sensor is simple to prepare, convenient to operate and low in cost and has the bright application prospect in the aspect of evaluation of the toxicity of the pathogenic bacteria.

Description

technical field [0001] The invention relates to the application of a gold nanometer-carbon nanotube-chitosan composite membrane cell sensor in the toxicity detection of food-borne pathogenic bacteria, and belongs to the technical field of toxicity detection of Gram-negative pathogenic bacteria. Background technique [0002] In recent years, with the acceleration of economic globalization, food safety has become a global public health hotspot. The hazards of food-borne pathogens are an important aspect of food safety, and will not decrease or disappear with economic development and technological progress. Food safety vicious incidents caused by food-borne pathogens have occurred one after another worldwide, and food-borne diseases The incidence rate remains high. Therefore, the toxicity evaluation of foodborne pathogens has become an important topic in the field of food safety. Lipopolysaccharides from Gram-negative bacteria have been found to be one of the most critical fa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/26G01N27/48G01N27/30
CPCG01N27/26G01N27/308G01N27/48
Inventor 孙秀兰王秀梅皮付伟张银志蒋卉纪剑
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com