Microecology preparation for cesarean delivery newborn baby microbial bath and preparation method of microecology preparation

A micro-ecological preparation and neonatal technology, applied in the field of microorganisms, can solve problems such as the impact on infant health, achieve the effects of inhibiting colonization, improving infant immunity, and increasing survival rate

Pending Publication Date: 2018-05-29
CHINA PHARM UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Babies delivered by cesarean section do not acquire maternally inherited microbiota, resulting in long-term, unhelpful or even harmful effects on the baby's health

Method used

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  • Microecology preparation for cesarean delivery newborn baby microbial bath and preparation method of microecology preparation

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Experimental program
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Effect test

Embodiment 1

[0023] Four strains of Bifidobacterium infantis CICC21716, Bifidobacterium bifidum CICC10395, Lactobacillus rhamnosus CGMCC1.2568, and Lactobacillus reuteri CICC10855 were cultured in the order of slant, liquid seed, and liquid fermentation, and slant and liquid fermentation culture The bases are respectively: Bifidobacterium infantis, Bifidobacterium bifidum, Lactobacillus rhamnosus, Lactobacillus reuteri culture medium is MRS medium, and its composition is (W / V): peptone 1%, beef powder 0.5% , yeast powder 0.4%, glucose 2%, Tween 80 0.1%, dipotassium hydrogen phosphate 0.2%, sodium acetate 0.5%, triammonium citrate 0.2%, magnesium sulfate 0.02%, manganese sulfate 0.005%, solid medium added to agar Powder 1.5-2%, pH 6.2 or so. The seed liquid is inoculated into the fermentation medium according to the inoculation amount of 5%, and Bifidobacterium infantis, Bifidobacterium bifidum, Lactobacillus rhamnosus, and Lactobacillus reuteri are anaerobically cultured at 37°C for 24-72h...

Embodiment 2

[0026] Four strains of Bifidobacterium infantis CICC21716, Bifidobacterium bifidum CICC10395, Lactobacillus rhamnosus CGMCC1.2568, and Lactobacillus reuteri CICC10855 were cultivated in the order of slant, liquid seed, and liquid fermentation, and slant and liquid fermentation culture The bases are respectively: Bifidobacterium infantis, Bifidobacterium bifidum, Lactobacillus rhamnosus, Lactobacillus reuteri culture medium is MRS medium, and its composition is (W / V): peptone 1%, beef powder 0.5% , yeast powder 0.4%, glucose 2%, Tween 80 0.1%, dipotassium hydrogen phosphate 0.2%, sodium acetate 0.5%, triammonium citrate 0.2%, magnesium sulfate 0.02%, manganese sulfate 0.005%, solid medium added to agar Powder 1.5-2%, pH 6.2 or so. The seed liquid is inoculated into the fermentation medium according to the inoculation amount of 5%, and Bifidobacterium infantis, Bifidobacterium bifidum, Lactobacillus rhamnosus, and Lactobacillus reuteri are anaerobically cultured at 37°C for 24-7...

Embodiment 3

[0029] Four strains of Bifidobacterium infantis CICC21716, Bifidobacterium bifidum CICC10395, Lactobacillus rhamnosus CGMCC1.2568, and Lactobacillus reuteri CICC10855 were cultivated in the order of slant, liquid seed, and liquid fermentation, and slant and liquid fermentation culture The bases are respectively: Bifidobacterium infantis, Bifidobacterium bifidum, Lactobacillus rhamnosus, Lactobacillus reuteri culture medium is MRS medium, and its composition is (W / V): peptone 1%, beef powder 0.5% , yeast powder 0.4%, glucose 2%, Tween 80 0.1%, dipotassium hydrogen phosphate 0.2%, sodium acetate 0.5%, triammonium citrate 0.2%, magnesium sulfate 0.02%, manganese sulfate 0.005%, solid medium added to agar Powder 1.5-2%, pH 6.2 or so. The seed liquid is inoculated into the fermentation medium according to the inoculation amount of 5%, and Bifidobacterium infantis, Bifidobacterium bifidum, Lactobacillus rhamnosus, and Lactobacillus reuteri are anaerobically cultured at 37°C for 24-7...

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Abstract

The invention discloses a composite microecology preparation for a cesarean delivery newborn baby to primarily acquire beneficial microbes and a preparation method of composite microecology preparation. The composite microecology preparation is prepared from bifidobacterium infantis, bifidobacterium bifidum, lactobacillus rhamnosus, and lactobacillus reuteri conventional in vagina of a healthy woman having a probiotic function, the probiotics are independently fermented in an appropriate culture medium, after the fermentation, the freeze-drying protective agent is added according to a given ratio, and the mixture is vacuum frozen dried, thus obtaining the microecology preparation. The microecology freeze-drying preparation has the characteristics of milk fragrance, high bacteria activity,capability of being stored for a long time, and the like. When the composite microecology preparation is used for performing the microbial bath for the cesarean delivery newborn baby, the bacterial colonization defect of the newborn baby caused by the cesarean delivery can be compensated, the probiotic microbial flora which is not obtained by the baby from the vagina of the mother can be partiallyrestored, so that the colonization of harmful bacteria after the birth of the cesarean delivery newborn baby can be effectively inhibited, the immunity of the baby can be improved, the maturity of the baby intestinal flora can be promoted, and the baby can be helped to establish normal flora and the like.

Description

technical field [0001] The invention patent belongs to the technical field of microorganisms. In particular, it relates to a compound microecological preparation for cesarean section neonatal microbial bath and a preparation method thereof. Background technique [0002] The colonization of neonatal microbiota after birth is affected by many factors, including the uterine environment before birth, the mode of delivery at birth, antibiotic treatment after birth, diet, and so on. The disturbance of the microbiome caused by these factors can cause the disturbance of the immune system and thus cause the disease. Early childhood is the "window period" for microbial colonization. Different modes of delivery are the primary factors affecting the formation of infant microflora. Early differences in the microbiota of infants delivered by cesarean section and infants born vaginally persist. The bacterial colonization of vaginally delivered infants is mainly affected by the mother's...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61K35/745A61K9/19A61K47/26A61K47/46A61P37/04
CPCA61K9/0014A61K9/19A61K35/745A61K35/747A61K47/26A61K47/46A61K2300/00
Inventor 储卫华周淑鑫
Owner CHINA PHARM UNIV
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