Method for tissue culture and rapid propagation of Rosa rugosa Thunb.

An edible rose, tissue culture rapid propagation technology, applied in the directions of horticultural methods, botanical equipment and methods, applications, etc., can solve the problem of no edible rose tissue, and achieve the effects of short cultivation cycle, simple method and low reproduction rate.

Inactive Publication Date: 2014-08-06
KUNMING UNIV
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Problems solved by technology

[0004] After literature search, there is no published content about tissue culture of edible roses

Method used

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  • Method for tissue culture and rapid propagation of Rosa rugosa Thunb.
  • Method for tissue culture and rapid propagation of Rosa rugosa Thunb.
  • Method for tissue culture and rapid propagation of Rosa rugosa Thunb.

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Embodiment Construction

[0023] The inventive method is aimed at Rosaceae Rosa deciduous upright shrub edible rose ( Rosa rugosa Thunb .), through the regulation of the four technical links of explant selection and disinfection, primary culture, proliferation culture, and rooting culture, it can effectively realize the rapid propagation of edible rose tissue culture. Specifically include the following steps:

[0024] A. Selection and disinfection of explants: Cut the young stems of edible roses that grow vigorously and are free of diseases and insect pests in April, remove the leaves, clean them with a soft brush dipped in a small amount of detergent, then cut them into small sections, place them in a beaker and wash them with running water Rinse for 2 hours, then transfer to the aseptic operating table for subsequent disinfection. Rinse with sterile water for 2-3 times, then soak in 75% ethanol for 30s, take it out, rinse it with sterile water for 3-4 times, then soak it in 0.1% mercury liter for 8m...

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Abstract

The invention discloses a method for tissue culture and rapid propagation of rosaceae Rosa deciduous erect shrub Rosa rugosa Thunb. belonging to the technical field of horticultural plant seedling propagation. The method is used for carrying out isolated culture on edible Rosa rugosa Thunb. caulicles to build a tissue culture and rapid propagation system of Rosa rugosa Thunb. and comprises four stages, namely selection and sterilization, primary culture, multiplication culture and rooting culture of an explant. The method disclosed by the invention is simple and has the advantages of high reproduction rate, short culture period and high reproduction coefficient, has relatively strong practicability in the actual production application, solves the technical problems of low reproduction rate and long reproduction period of a Rosa rugosa Thunb. seedling, and is suitable for commercial production of Rosa rugosa Thunb. seedlings so as to meet the urgent demand of a market.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of edible roses, in particular to a method for tissue culture and rapid propagation of edible rose young stems, belonging to the technical field of horticultural plant seedling propagation. Background technique [0002] edible rose ( Rosa rugosa Thunb .) Rosaceae Rosa, native to China, is a deciduous erect shrub. At present, it is widely cultivated not only in China, but also in Japan, North Korea, Europe and the United States. It is widely used in medicine, industry, food, daily chemical industry and other fields. In recent years, the development of edible roses in Yunnan Province is quite rapid. Its good edible value and ornamental value make edible roses favored by many people, resulting in a rapid increase in demand and a broad market prospect. At present, the area of ​​edible roses in Yunnan Province is about 10,000 mu, and the number of rose processing enterprises is in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈泽斌靳松黄鹤平黄萍刘佳妮张永福余磊华金珠莫丽玲郑旴
Owner KUNMING UNIV
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