215results about How to "Rapid cultivation" patented technology

The invention discloses a method for reshaping pear trees, relating to a method for reshaping fruit trees and comprising the following steps: (1) determining reasonable plantation density, wherein appropriately, the line spacing is 4m and the row spacing is 5-6m; (2) formulating an overall objective for culturing tree shapes, controlling the trunk height of a pear tree to be 0.7-0.8m and the distance away from the ground to be 0.8m, allocating two boughs along a progressive direction, and inducing horizontally; and allocating lateral branches at the two sides of the boughs, performing line spacing inducement through a mode for forming a dip angle of 45 degrees with the horizontal plane, and controlling the spacing between lateral branches to be 0.4-0.5m; (3) realizing tree shape culture by the steps of bough culturing and inducing, and reasonable allocating and trimming of lateral branches; and (4) constructing a framework adaptive to labor-saving reshaping, wherein the pear trees are V-shaped and U-shaped. The invention has the advantages as follows: (1) the reshaping technology has simple requirements and rapid speed; (2) the field operation is easy and has high efficiency; (3) the expansion of operation to an appropriate scale per capita is possible; and (4) the early-stage yielding ability is good.

The invention discloses a wastewater treating microbial agent and a preparation method thereof. The microbial agent contains kocuriapalustris FSDN-A, arthrobactercreatinolyticus FDN-1, flavobacteriummizutaii FDN-2, paracoccusdenitrificans DN-3 and methylobacteriumphyllosphaerae SDN-3, wherein the collection register numbers of the five strains are respectively CGMCCNo.5061, CGMCCNo.3657, CGMCCNo.3659, CGMCCNo.3658 and CGMCCNo.3660. The microbial agent can achieve removal of ammonia nitrogen, total nitrogen and CODcr in the same reactor, has a good wastewater treatment effect, and can achieve short-cut nitrification and denitrification or simultaneous nitrification and denitrification while removing COD. Kocuriapalustris FSDN-A can utilize the two nitric oxides including nitrite nitrogen and nitric nitrogen, can ensure thorough denitrification of a wastewater treatment system, is slow in appreciation in the process of competing for substrates, is favorable for reduction of sludge, and further widens the application range of the microbial agent.

The invention discloses a high slender spindle-shaped apple tree form and a trimming method thereof. As a whole, the tree form is of high slender spindle shape. After an apple tree is formed, the crown diameter of the apple tree is small, slender and high. The apple tree is 3.5-4.0m high and the trunk of the apple tree is 0.8-0.9m high. Thirty to fifty spirally arranged small boughs are grown on the central leading trunk of the apple tree. The average length of the small boughs is 1m. The average angle between the central trunk and the small bough is 115 degrees. The top-to-bottom spacing among the small boughs on the same side is 0.25m. The trimming method of the high slender spindle-shaped tree form is simple. Other boughs require no decapitation but branch drawing except that the central trunk requires short-cutting in winters in 1-2 years. Thereby, the trimming method can be popularized in tree forms of simplified trimming and low labor strength.

The invention relates to the technical field of biology; in order to solve the deficiency of wild phellinus igniarius resources at present, and the problems of insufficient medium nutrition supply, small sporophore growth, and easy undesired microbe infection of bag cultivation in artificial cultivation, the invention provides a phellinus igniarius bag cultivation medium and a method for cultivating phellinus igniarius sporophores by using the medium; the phellinus igniarius bag cultivation medium is prepared by mixing nutrients with water, packaging the mixture in bags, sterilizing the bags at a temperature of 110-135 DEG C, at a pressure of 0.05-0.25 MPa, for 30-180 min, wherein the mass ratio of the nutrients to water is 1:1.8-2.5. With the phellinus igniarius bag cultivation medium formula of the invention, nutrients absorbed during phellinus igniarius sporophore growth are provided, and the cultivated phellinus igniarius sporophores are similar to wild phellinus igniarius with stable quality; and the cultivation mode of the invention is simple, and can be used for large scale cultivation of phellinus igniarius sporophores with medicinal value.

The invention discloses a nitrite bacteria growth promoter including the components: 40-100 parts by weight, preferably 50-80 parts by weight, of metal salts; 5-30 parts by weight, preferably 10-20 parts by weight, of an amine substance; 0.05-1.5 parts by weight, preferably 0.1-1.0 part by weight, of organic acid hydroxylamine; and 10-40 parts by weight, preferably 20-30 parts by weight, of Na2SO3; the metal salts comprise a calcium salt, a magnesium salt, a ferrous salt and a copper salt, and the molar ratio of Ca<2+> to Mg<2+> to Fe<2+> to Cu<2+> is (5-15) to (5-25) to (1-8) to (0.5-5), preferably (8-12) to (10-20) to (2-6) to (1-4). The promoter has the advantages of simple formula and easy preparation, can be used in the cultivation process of nitrite bacteria, can also be directly added into a sewage treatment system, accelerates starting of short-cut nitrification-denitrification and short-cut nitrification-anaerobic ammonium oxidation processes and achieves stable operation.

The invention discloses a nitrite bacterium culture promoter, which consists of metal salts, polyamine substances, organic acid hydroxylamine and Na2SO3, wherein in parts by weight, the content of the metal salts is 40-100 parts by weight, preferably 50-80 parts by weight; the content of the polyamine substances is 5-30 parts by weight, preferably 10-20 parts by weight; the content of the organic acid hydroxylamine is 0.05-1.5 parts by weight, preferably 0.1-1.0 part by weight; and the content of the Na2SO3 is 10-40 parts by weight, preferably 20-30 parts by weight; the metal salts include calcium salt, ferrous salt and copper salt; and the molar ratio of Ca<2+> to Fe<2+> to Cu<2+> is (5-15) to (1-8) to (0.5-5), and preferably (8-12) to (2-6) to (1-4). The promoter is simple in formula and easy for preparing; and the promoter can be applied to the culture process of the nitrite bacteria and the promoter can be also directly added to a sewage treatment system, so that the starting of partial nitrification-denitrification and partial nitrification-anaerobic ammonia oxidation processes is accelerated and the stable running of the processes is achieved.

The invention discloses a nitrifying bacterium culture promoter, which consists of metal salts, polyamine substances and organic acid hydroxylamine, wherein in parts by weight, the content of the metal salts is 40-100 parts by weight, preferably 50-80 parts by weight; the content of the polyamine substances is 5-30 parts by weight, preferably 10-20 parts by weight; and the content of the organic acid hydroxylamine is 0.5-15 parts by weight, preferably 2-10 part by weight; the metal salts include calcium salt, magnesium salt, ferrous salt and copper salt; and the molar ratio of Ca<2+> to Mg<2+> to Fe<2+> to Cu<2+> is (5-15) to (5-25) to (1-8) to (0.5-5), and preferably (8-12) to (10-20) to (2-6) to (1-4). The promoter is simple in formula and easy for preparing; and the promoter can be applied to the culture process of the nitrifying bacteria and the promoter can be also directly added to a sewage treatment system, so that the rapid growth and reproduction of nitrifying bacteria can be achieved within a short time; and the cultured nitrifying bacteria are high in activity and strong in impact resistance.

The present invention discloses a denitrification microorganism culture accelerator, which comprises a metal salt, a polyamine substance, an organic acid hydroxylamine and an organic acid salt, wherein the metal salt comprises a calcium salt, a copper salt, a magnesium salt and / or a ferrous salt. The preparation method comprises: (1) preparing a metal salt solution according to a certain composition and a certain weight part ratio; and (2) adding a polyamine substance, an organic acid hydroxylamine and an organic acid salt into the metal salt solution before use. According to the present invention, the denitrification microorganism culture accelerator has advantages of simple formula and easy preparation, the cultured denitrifying microorganisms have characteristics of high activity and strong impact resistance, and can perform denitrogenation reactions by using nitrite nitrogen as the electron acceptor, and the accelerator can be used in the denitrification microorganism culture process using nitrite nitrogen as the electron acceptor and can further be directly poured into the existing sewage treatment system so as to promote the smooth operating of the short-term nitrification and denitrification process.

The invention discloses a method for reconstructing submerged plant communities of lakeside wetland and controlling water quality purification. The method comprises the steps of monitoring and analyzing the water quality purification capacity and the community change rule of water plants in an ecological restoration area of a lakeside wetland, and determining some kinds of water plants with high-intensity purification and survival ability to serve as pioneer species; determining which kind of season dominant species to which the pioneer species belong respectively, and constructing submerged plant communities of the lakeside wetland through reasonable matching of different season dominant species; quickly cultivating the pioneer species on a large scale in the ecological restoration area of the lakeside wetland to realize purification control of water quality. Compared with the prior art, by adopting the method disclosed by the invention, the amount of the submerged plant communities of the lakeside wetland can be effectively controlled, and the stability of a plant cover in an eutrophic water body is maintained, so that the method is of great significance to ecological restoration of the eutrophic water body; moreover, the method disclosed by the invention is technically reliable, simple and feasible in operation and low in cost.

The invention discloses a denitrification method by sewage short-cut simultaneous nitrification and denitrification, comprising the following step of: adding a nitrous acid denitrifying inoculant into sewage containing ammonia and nitrogen, wherein the ammonia-nitrogen-containing sewage treatment temperature is 18-40 DEG C, dissolved oxygen is 0.1-3mg / L, and pH is 7-9. According to the method, the heterotrophic nitrobacteria are used for advantage combination as the enhanced microorganism to process wastewater, thus realizing the removal of ammonia nitrogen, total nitrogen and CODcr in a samereactor. The effect of wastewater treatment is good. The method provided by the invention is especially suitable for the purification treatment of wastewater containing ammonia nitrogen, and can be used to actually realize denitrification by short-cut nitrification and denitrification or simultaneous nitrification and denitrification.

The invention relates to a salt-tolerant COD removal denitrifying microbial agent. The microbial agent consists of at least one of paracoccus sp FSTB-2, microbacterium kitamiense FSTB-4 and pseudomonas stutzeri FSTB-5, and further consists of at least one of paracoccus denitrificans DN-3 and methylobacterium phyllosphaerae SDN-3, wherein the paracoccus sp FSTB-2, the microbacterium kitamiense FSTB-4 and the pseudomonas stutzeri FSTB-5 are preserved in China General Microbiological Culture Collection Center on June 1, 2015, with preservation numbers of CGMCC No.10938, CGMCC No.10939 and CGMCC No.10940; and the paracoccus denitrificans DN-3 and the methylobacterium phyllosphaerae SDN-3 are disclosed in CN102465104A and CN102465103, and the preservation numbers of the paracoccus denitrificans DN-3 and the methylobacterium phyllosphaerae SDN-3 are CGMCC No.3658 and CGMCC No.3660. The microbial agent can be used for directly processing total nitrogen and COD in high-salinity wastewater, and the microbial agent can be also added to various biochemical reaction constructions so as to improve microbiological composition, optimize the salt tolerance of a microbial system in wastewater treatment and improve total nitrogen and COD removal rate of the entire process.

An efficient aggregate method for breeding seedless grap is disclosed, which features that the conventional hybridizing technique, the embryo retriever technique and molecular marker breeding technique are integrated. Its advantages are short breeding period shortened from 7-9 years to 3-4 months, high resistance to disease, high seedless percentage, and high quality of grape.

The invention discloses a data annotation management method and device, electronic equipment and a readable storage medium. The method comprises the steps: obtaining a reference annotation data set according to to-be-annotated data corresponding to a to-be-annotated task and historical annotation behavior data corresponding to a target annotator; obtaining a first annotation result of the target annotator for the evaluation annotation data and first reference annotation data distributed in the evaluation annotation data, the evaluation annotation data being a part of the to-be-annotated data and having a correct annotation answer, and the first reference annotation data belonging to a reference annotation data set; If the accuracy corresponding to the first annotation result is greater than or equal to a preset accuracy threshold, determining whether to allow the target annotator to continue to execute the to-be-annotated task or not according to a second annotation result of the target annotator for second reference annotation data already distributed in the to-be-annotated data, the second reference annotation data belonging to a reference annotation data set. According to the embodiment of the invention, the quality and efficiency of data annotation can be improved.

The invention discloses a composition for rapidly culturing nitrifying bacteria. The composition comprises the following components in parts by weight: 40-100 parts of metal salts, preferably 50-80 parts of metal salts, 5-30 parts of polyamine substance, preferably 10-20 parts of polyamine substance, and 0.5-15 parts of hydroxylamine organic acid, preferably 2-10 part of hydroxylamine organic acid, wherein the metal salts comprise calcium salt, magnesium salt and copper salt, and the molar ratio of Ca<2+> to Mg<2+> to Cu<2+> is (5-15) to (5-25) to (0.5-5), preferably (8-12) to (10-20) to (1-4). The composition is simple in formula and easy to prepare, can be used in a culture process of nitrifying bacteria, and also can be directly fed into a sewage treatment system, so that the nitrifying bacteria can rapidly grow and breed within a short time, and the cultured nitrifying bacteria have high activity and strong impact tolerance.

A tissue culture method for quickly reproducing the red cactus includes such steps as disinfecting the tender leaf of red cactus, pretreating in the pretreating liquid containing BA and 2,4-D, cutting to become blocks, inductive culture in the improved MS culture medium to induce calli, differentiating adventitious buds in the improved diferential culture medium, culturing the rooted aseptic seedlings in the improved reproductive culture medium, naturalizing, and transplanting them in pots.

The invention relates to a Lecanicilliumpsalliotae strain used for preventing and treating diaphorina citri and belongs to the technical field of microorganisms. The Lecanicilliumpsalliotae strain ZJLP09 is registered and preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCCNo.7551. The Lecanicilliumpsalliotae strain ZJLP09 has strong pathogenicity on diaphorina citri; compared with a congeneric verticillum lecanii strain, the Lecanicilliumpsalliotae strain ZJLP09 has a stronger effect on the diaphorina citri and can be developed into a biological fungi-proofing preparation used for preventing and treating the diaphorina citri; and compared with the existing chemical agent used for preventing and treating the diaphorina citri, the Lecanicilliumpsalliotae strain ZJLP09 has the characteristics of high efficiency, low toxicity, low residue, no pollution and difficulty in formation of drug resistance and has a good market application prospect.

The invention discloses a rapid breeding method of lamina seedlings of tinospora capillipes gagnep. The method comprises the steps: (1) scratching the back sides of tender laminas of sterile test tube seedlings of tinospora capillipes gagnep to be well-shaped by using a scalpel and putting into an MS propagation medium for culturing; performing induction formation on callus and classifying to obtain multiple shoots; (2) putting the multiple shoots into an MS strong seedling medium for culturing strong seedlings to obtain strong plants; and (3) putting the strong plants into a 1 / 2 MS rooting medium to obtain complete root seedlings. The proliferation coefficient of the multiple shoots of the tinospora capillipes gagnep obtained by using the culturing method reaches 20-30 times, the rooting rate of the obtained tissue culture seedlings is more than 95 percent, the survival rate of seedbed transplanting is more than 90 percent, and the scale seedling breeding problem of the tinospora capillipes gagnep is effectively solved.

The invention discloses an asexual rapid propagation method for idesia seedlings. The asexual rapid propagation method mainly comprises the steps of screening stock plants, cutting orchard and preprocessing, cutting orchard and cutting, raising seedlings and managing, taking the seedling out of gardens and performing subculture. Compared with the tissue culture in the prior art, the asexual rapid propagation method for idesia seedlings is simple in step, low in requirements of technology and devices and low in cost; and meanwhile, the culture of the idesia seedlings adopts branches of the excellent stock plants, so that the large-scale rapid propagation can be realized.

The invention discloses a cultivation method for phellinus igniarius strains from solid culture medium to liquid culture medium, which is mainly used for solving the problems of the existing phelllinus igniarius mother strain cultivation method that the mycelium germination speed is low, the pollution rate is high and the flavones yield is relatively low. The method comprises the following steps: (1) acquiring a phellinus igniarius mother strain, and inoculating the phellinus igniarius mother strain into a solid culture medium to be cultured in a light-proof manner; and (2) inoculating the strain in the solid culture medium into a liquid culture medium, and culturing the strain to obtain phellinus igniarius liquid strain, wherein the solid culture medium comprises the following components by weight percent: 20 to 30 percent of mulberry sawdust, 40 to 50 percent of xylosma sawdust, 20 to 27 percent of wheat bran, 1 to 1.5 percent of brown sugar, 0.2 percent of monopotassium phosphate, 0.1 percent of magnesium sulfate, and 1 to 1.5 percent of gypsum powder; and the water content is 60 to 63 percent. The cultivation method has the advantages that the growth speed can be increased by 30 to 40 percent, the flavones yield is increased by 20 percent, the yield is high, the sporocarp quality is good, the technology is complete, the reusability is realized, the economic benefit is good, and the like.

The invention discloses a promoter for culturing ammonia oxidizing bacteria. The promoter comprises the following components in parts by weight: 40-100 parts of metal salts, preferably 50-80 parts of metal salts, 5-30 parts of polyamine substance, preferably 10-20 parts of polyamine substance, 0.05-1.5 parts of hydroxylamine inorganic acid, preferably 0.1-1.0 part of hydroxylamine inorganic acid, and 10-40 parts of Na2SO3, preferably 20-30 parts of Na2SO3, wherein the metal salts comprise calcium salt, magnesium salt and copper salt, and the molar ratio of Ca<2+> to Mg<2+> to Cu<2+> is (5-15) to (5-25) to (0.5-5), preferably (8-12) to (10-20) to (1-4). The promoter is simple in composition and easy to prepare, can be used in a culture process of ammonia oxidizing bacteria, and also can be directly fed into a sewage treatment system, so that the starting of short-cut nitrification-denitrification and short-cut nitrification-anaerobic ammonia oxidation processes is accelerated, and the stable operation is realized.

The invention provides a detection vessel capable of rapidly culturing and detecting moulds and yeasts at the same time. A biochemical reaction characteristic of microorganisms is utilized, types and reaction conditions of endoenzymes of the microorganisms are determined as main bases for microorganism classification and identification, a microorganism specific enzyme chromogenic substrate is added into an isolation medium or a selective medium, and when target bacteria grow on the medium, the chromogenic substrate can be degraded by specific enzymes and metabolites in special colors can be generated to realize specific colors or forms of colonies, thereby implementing culturing and detection of the microorganisms in a sample. The detection vessel has the advantages of convenience in operation, low cost, labor saving, simple judgment result, reliable detection result, short detection time and the like, and can play an important role in microorganism detection, and accuracy of a detection result reaches 90 percent or over 90 percent of a national standard detection method.

The invention discloses an ammonia oxidizing bacterium growth promoter, which consists of metal salts, polyamine substances, inorganic acid hydroxylamine and Na2SO3, wherein in parts by weight, the metal salts account for 40-100 parts by weight, preferably 50-80 parts by weight; the polyamine substances account for 5-30 parts by weight, preferably 10-20 parts by weight; the inorganic acid hydroxylamine accounts for 0.05-1.5 parts by weight, preferably 0.1-1.0 part by weight; and the Na2SO3 accounts for 10-40 parts by weight, preferably 20-30 parts by weight; the metal salts include calcium salt, ferrous salt and copper salt; and the molar ratio of Ca<2>+ to Fe<2+> to Cu<2+> is (5-15) to (1-8) to (0.5-5), and preferably (8-12) to (2-6) to (1-4). The growth promoter is simple in formula and easy to prepare; and the growth promoter can be applied to the culture process of ammonia oxidizing bacteria and can be also directly added to a sewage treatment system, so as to accelerate the starting of a partial nitrification-denitrification and partial nitrification-anaerobic ammonia oxidation process and to achieve stable running.

The invention discloses a tissue culture rapid propagation method of plumbago auriculata. A plumbago auriculata in-vitro rapid propagation system is established by using a stem as an explant so as to provide technical support for the industrial production of the plumbago auriculata tissue culture. The stem is used as the explant to realize the tissue culture rapid propagation of the plumbago auriculata through the processes of single-bud induction, multiple shoot proliferation, rooting culture and test-tube plantlet domestication transplantation; the implement of the tissue culture rapid propagation method can rich the flower variety and bring about economic benefit for the industrial seedling, and the method further can provide research material for the cultivation of plumbago auriculata new variety.

The invention discloses a method for tissue culture and rapid propagation of rosaceae Rosa deciduous erect shrub Rosa rugosa Thunb. belonging to the technical field of horticultural plant seedling propagation. The method is used for carrying out isolated culture on edible Rosa rugosa Thunb. caulicles to build a tissue culture and rapid propagation system of Rosa rugosa Thunb. and comprises four stages, namely selection and sterilization, primary culture, multiplication culture and rooting culture of an explant. The method disclosed by the invention is simple and has the advantages of high reproduction rate, short culture period and high reproduction coefficient, has relatively strong practicability in the actual production application, solves the technical problems of low reproduction rate and long reproduction period of a Rosa rugosa Thunb. seedling, and is suitable for commercial production of Rosa rugosa Thunb. seedlings so as to meet the urgent demand of a market.

The invention relates to a method used to detect estrogen activity contribution matter in sewage. It includes the following steps: extracting estrogen activity matter form the sewage sample by solid phase column extractor; using methanol, dichloroethanes, normal hexane purging, and silica gel chromatographic column to classify into polarity, moderate and weak polarity three components; using recombination gene yeast bioassay method to measure the estrogen activity; taking out the maximum component to analyze its kind and density by gas phase color photography-mass spectrum on line. This method can fast effectively detect the main estrogen activity contribution matter in the sewage, supply foundation and support for sewerage treatment technology improvement.

The invention relates to a tissue culture method for plants, and in particular relates to a method for tissue culture of liquidambar styraciflua. The method comprises the following steps: selecting effective stems with axillary buds of liquidambar styraciflua as an explant, performing pretreatment such as sterilization on the effective stems firstly, subsequently inoculating the explant onto an axillary bud development starting culture medium for culture on an ultra-clean working table so as to obtain sterile seedlings; inoculating the sterile seedlings into a bud proliferation and elongation culture medium for culture, selecting growing points of the proliferated buds, sequentially transferring into a callus induction culture medium, a callus proliferation culture medium and a root induction culture medium for culture according to culture progress so as to obtain non-poisonous plants, and transplanting and planting the non-poisonous plants. According to the method, the unique explant and the formulae of the culture mediums are adopted, the success rate and the plant quality in tissue culture of liquidambar styraciflua are greatly improved, and strong guarantee for industrial development of liquidambar styraciflua is provided, and the method is rapid in culture and economical.

The invention belongs to the technical field of in-vitro culture cells. An adipose cell (normal) / hepatic cell co-culture model and an adipose cell (inflammation) / hepatic cell co-culture model are respectively set up to be applied to quick screening of lipid-lowering drugs. An adipose cell / hepatic cell co-culture system in the invention includes that in a Millicell double-layer culture system, hepatic cells are cultured on a translucent film of an upper culture chamber by attaching on walls, and adipose cells are cultured on the bottom surface of a lower culture chamber by attaching on walls. The adipose cell / hepatic cell co-culture system simulates control action of adipose tissue on lipid metabolism of the liver in vitro, and further is applied to quick screening of the lipid-lowering drugs according to affection of the drugs on cell factors, enzyme and acceptors.

The invention provides a tissue culture and rapid propagation method for corydalis saxicola bunting. The tissue culture and rapid propagation method comprises the following steps: (1) taking a corydalis saxicola bunting lateral bud as an explant and disinfecting; (2) putting the disinfected explant into an MS (Murashige and Skoog) culture medium to be induced and burgeoned to obtain cluster buds; (3) putting the cluster buds into an MS strong culture medium to be subjected to strong seedling culture to obtain a strong plant; (4) putting the strong plant into an MS rooting culture medium to be cultured to obtain a complete seedling with roots; and (5) taking the complete seedling with the roots and carrying out domestication; and transplanting the seedling into a sand bed to grow for one and a half months and then transplanting the seedling to a large field. With the adoption of the culture method, an obtained germchit is strong and the survival rate is high; a lot of high-quality germchits of the corydalis saxicola bunting can be provided in short time so that the large-scale seedling growth problem of the corydalis saxicola bunting is solved effectively.

The invention relates to a tissue culture rapid-multiplying method for bletilla striata protocorm-like bodies. The method comprises the following steps: (1) slitting bletilla striata and sterile test-tube plantlets into single corms with plantlets, inoculating to an MS protocorm-like body multiplying culture medium for multiplying culture to obtain protocorm-like bodies; (2) putting the obtained protocorm-like bodies into an MS rejuvenation culture medium for rejuvenation culture to obtain protocorms with seedlings; and (3) inoculating the protocorms with seedlings onto a 1 / 2 MS rooting culture medium for rooting culture to obtain small robust plants. The bletilla striata protocorm-like bodies obtained by adopting the tissue culture rapid-multiplying method are high in rejuvenation coefficient, short in rejuvenation time, robust for the rejuvenated multiple buds, the high-quality bletilla striata seedlings with high survival rate can be provided within short period, and the large-scale seedling culture problem of the bletilla striata can be effectively solved.