Tissue culture rapid propagation method of plumbago auriculata

A tissue culture, snowflake technology, applied in the field of plant tissue culture, can solve the problems of no report, lack, low multiplication coefficient, etc.

Inactive Publication Date: 2015-02-04
梁彩英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low seed setting rate of Blue Snowflake, expensive seeds, and low multiplication coefficient, it is impossible to meet the needs of Blue Snowflake seedlings by seed propagation.
In addition, due to the lack of experimental materials, the current research on the blue snowflake is seriously lagging behind, and there is no report on the research on the tissue culture of the blue snowflake plant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Single-bud induction: select annual tender branches with good growth and no diseases, soak them in aqueous solution of detergent for 10 minutes, rinse them in tap water for 30 minutes, soak them in 75% ethanol solution for 10 seconds in an ultra-clean workbench, and rinse them with sterile water for 3 minutes. sterilized with 0.1% mercuric chloride solution for 5 minutes, rinsed with sterile water for 3 times, blotted the water on the surface with sterile filter paper, cut off the leaves on the branches, cut them into 1cm stem segments, and inoculated them in single-bud induction Buds can be produced by culturing in the culture medium for 32 days under the conditions of 10 hours of light per day, light intensity of 1500x, and culture temperature of 25°C. Described stem segment is to be bounded by the stem node, and the upper half retains 0.4cm, and the length of the lower half retaining 0.6cm is a 1cm segmented stem segment. The single bud induction medium is: MS+0....

Embodiment 2

[0022] (1) Single-bud induction: select annual tender branches with good growth and no diseases, soak them in an aqueous solution of detergent for 20 minutes, rinse them in tap water for 40 minutes, soak them in 78% ethanol solution for 13 seconds in an ultra-clean workbench, and rinse them with sterile water for 5 minutes. and then sterilized with 0.3% mercuric chloride solution for 7 minutes, rinsed with sterile water for 5 times, blotted the water on the surface with sterile filter paper, cut off the leaves on the branches, cut them into 1cm stem segments, and inoculated them in single-bud induction The culture medium is cultivated for 30 days under the conditions of 12 hours of light per day, light intensity of 2000×, and culture temperature of 25° C. to produce buds. Described stem segment is to be bounded by the stem node, and the upper half retains 0.4cm, and the length of the lower half retaining 0.6cm is a 1cm segmented stem segment. The single bud induction medium is...

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PUM

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Abstract

The invention discloses a tissue culture rapid propagation method of plumbago auriculata. A plumbago auriculata in-vitro rapid propagation system is established by using a stem as an explant so as to provide technical support for the industrial production of the plumbago auriculata tissue culture. The stem is used as the explant to realize the tissue culture rapid propagation of the plumbago auriculata through the processes of single-bud induction, multiple shoot proliferation, rooting culture and test-tube plantlet domestication transplantation; the implement of the tissue culture rapid propagation method can rich the flower variety and bring about economic benefit for the industrial seedling, and the method further can provide research material for the cultivation of plumbago auriculata new variety.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a rapid propagation method for tissue culture of blue snowflakes. Background technique [0002] Blue Snowflake ( Plumbago auriculata ), also known as blue flower Dan, blue jasmine, blue snow red, etc., is a perennial evergreen shrub of the genus Baihuadan in the family diphtheria, a new type of flower with good ornamental value and medicinal value. Blue Snow Flower is rich in plumbagin, which has a good therapeutic effect on rheumatoid arthritis, blood stasis, amenorrhea, bruises, swollen sores and scabies. [0003] Blue Snowflake is a new type of flower that combines ornamental value and medicinal value, and has a good market application prospect. Due to the low seed setting rate of Blue Snowflake, high seed price and low multiplication coefficient, the demand for seedlings of Blue Snowflake cannot be satisfied by seed propagation. In addi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 梁彩英
Owner 梁彩英
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