Promoter for culturing ammonia oxidizing bacteria and preparation method of promoter

A technology of ammonia oxidizing bacteria and accelerators, applied in chemical instruments and methods, aerobic and anaerobic process treatment, and adding compounds to stimulate growth, etc., can solve the problems of no promotion, slow growth of ammonia oxidizing bacteria, etc. Achieve the effects of accelerating cell growth, improving the effect of wastewater treatment, and shortening the process of enzymatic reactions

Active Publication Date: 2016-06-01
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

CN201110315549.6 discloses a short-range nitrification and denitrification granular sludge cultivation method, which is characterized in that 5-15mg / L hydroxylamine is regularly added; CN201010168453.7 discloses a method for quickly starting an anoxic ammonia oxidation biofilter, It is characterized in that hydroxylamine is added in step (2) to induce the inoculated sludge to transform into anoxic ammonium oxidation biofilm. The above invention mainly utilizes the inhibitory effect of hydroxylamine on nitrite oxidizing bacteria to promote short-term nitrification, but slow growth The ammonia oxidizing bacteria did not promote their own growth

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  • Promoter for culturing ammonia oxidizing bacteria and preparation method of promoter
  • Promoter for culturing ammonia oxidizing bacteria and preparation method of promoter

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Embodiment Construction

[0018] The solutions of the present invention will be described in detail below in conjunction with specific embodiments.

[0019] See Table 1 for the accelerator formulations for the cultivation of ammonia oxidizing bacteria in Examples 1-4 and Comparative Examples 1-6.

[0020] Formulation and ratio of accelerator of table 1

[0021]

[0022] The ammonia nitrogen concentration in the waste water produced by a certain enterprise is 150mg / L, which is treated by the SBR process, and the accelerator of the present invention is added in the start-up stage of the device. Using the accelerator configured in the above table, the concentration is 0.5g / L, and the accelerator concentration in the sewage is added every day at 15mg / L. After 15 days of adding, the nitrosation rate in the aeration stage increases to more than 60%. Dosing was stopped, and the system continued to run for 10 days. Water samples were taken to analyze the concentration of ammonia nitrogen, nitrate nitrogen ...

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Abstract

The invention discloses a promoter for culturing ammonia oxidizing bacteria. The promoter comprises the following components in parts by weight: 40-100 parts of metal salts, preferably 50-80 parts of metal salts, 5-30 parts of polyamine substance, preferably 10-20 parts of polyamine substance, 0.05-1.5 parts of hydroxylamine inorganic acid, preferably 0.1-1.0 part of hydroxylamine inorganic acid, and 10-40 parts of Na2SO3, preferably 20-30 parts of Na2SO3, wherein the metal salts comprise calcium salt, magnesium salt and copper salt, and the molar ratio of Ca<2+> to Mg<2+> to Cu<2+> is (5-15) to (5-25) to (0.5-5), preferably (8-12) to (10-20) to (1-4). The promoter is simple in composition and easy to prepare, can be used in a culture process of ammonia oxidizing bacteria, and also can be directly fed into a sewage treatment system, so that the starting of short-cut nitrification-denitrification and short-cut nitrification-anaerobic ammonia oxidation processes is accelerated, and the stable operation is realized.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an accelerator for cultivating ammonia oxidizing bacteria and a preparation method. Background technique [0002] Biological denitrification is mainly accomplished by nitrifying bacteria and denitrifying bacteria. Nitrifying bacteria are chemotrophic microorganisms. Biological cells can only use the energy stored in the form of ATP, etc., and cannot directly use the free energy released by chemical reactions. In aerobic metabolism, ATP is mainly synthesized by oxidative phosphorylation of the respiratory chain. The ammonia oxidative phosphorylation efficiency is very low, and the ATP that can be produced is very limited, and the energy is mainly used for electron transition to a higher energy level, which makes the growth of nitrifying bacteria very slow, and the generation period is 8~36h. The cell wall of nitrifying bacteria has low peptidoglycan content and high pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C02F3/34C02F3/30
Inventor 高会杰孙丹凤郭志华赵胜楠郭宏山
Owner CHINA PETROLEUM & CHEM CORP
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