Model for quickly screening lipid-lowering drugs by adipose cell/hepatic cell co-culture model and application of model for quickly screening lipid-lowering drugs

Abstract

The invention belongs to the technical field of in-vitro culture cells. An adipose cell (normal)/hepatic cell co-culture model and an adipose cell (inflammation)/hepatic cell co-culture model are respectively set up to be applied to quick screening of lipid-lowering drugs. An adipose cell/hepatic cell co-culture system in the invention includes that in a Millicell double-layer culture system, hepatic cells are cultured on a translucent film of an upper culture chamber by attaching on walls, and adipose cells are cultured on the bottom surface of a lower culture chamber by attaching on walls. The adipose cell/hepatic cell co-culture system simulates control action of adipose tissue on lipid metabolism of the liver in vitro, and further is applied to quick screening of the lipid-lowering drugs according to affection of the drugs on cell factors, enzyme and acceptors.

Description

technical field [0001] The invention belongs to the technical field of cell culture in vitro, and in particular relates to a model for rapid screening of lipid-lowering drugs in a fat cell / hepatocyte co-culture model and its application Background technique [0002] With the development of the economy and the change of the national lifestyle, the incidence of a series of disorders of lipid metabolism, such as hyperlipidemia, non-alcoholic fatty liver, and metabolic syndrome, is increasing day by day. For example, the 2002 survey of Chinese residents and health showed that there were 160 million people with dyslipidemia in my country, while the "Guidelines for the Prevention and Treatment of Dyslipidemia in Chinese Adults" released in 2007 showed that the prevalence rate of dyslipidemia in China rose rapidly to 18.6%. In recent years, the incidence of fatty liver has also risen rapidly, especially in some occupational groups (white-collar workers, taxi drivers, professional m...

AI Technical Summary

Problems solved by technology

The above-mentioned in vitro model can not comprehensively simulate the lipid metabolism in vivo, and it is defective as an evaluation method for drug screening.

The in vivo animal model evaluation methods for drugs that regulate lipid metabolism disorders are mainly used: such as the establishment of a rabbit high-fat diet-induced hyperlipidemia model, a rat high-fat diet-induced non-alcoholic fatty liver model, etc. Although animal models can be more appropriate Simulate the situation of abnormal lipid metabolism, but the cycle is long and expensive, and it is not suitable for rapid evaluation research in the early discovery stage of drugs

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