46 results about "Culture model" patented technology

A method for presenting a perspective view of a real urban environment, augmented with associated geo-coded content, and presented on a display of a terminal device. The method comprises the steps of: connecting the terminal device to a server via a network; communicating user identification, user present-position information and at least one user command, from the terminal device to the server; processing a high-fidelity, large-scale, three-dimensional (3D) model of an urban environment, and associated geo-coded content by the server; communicating the 3D model and associated geo-coded content from said server to said terminal device, and processing said data layers and said associated geo-coded content, in the terminal device to form a perspective view of the real urban environment augmented with the associated geo-coded content. The 3D model comprises a data layer of 3D building models; a data layer of terrain skin model; and a data layer of 3D street-level-culture models. The processed data layers and the associated geo-coded content correspond to the user present-position, the user identification information, and the user command.

The invention discloses a feeding method of culturing the baby hippocampus trimaculatus leach. The technical method designs a screening and converting method of the fish bait type in different growth time suitable for the baby Hippocampus Trimaculatus Leach based on the baby's ingestion and life habits. The technical method changes the conventional culture model of the baby hippocampus trimaculatus leach and can remarkably increase the growth rate and the survival rate of the baby hippocampus trimaculatus leach.

The invention provides a plant culture method and system based on IoT (Internet of things) big data analysis, wherein the method comprises the steps of collecting plant species, soil humidity, a soil pH value, illumination intensity, environment temperature, environment humidity, images, watering quantity, fertilizing amount and fertilizing types; forming influence factor matrices X; uploading the influence factor matrices X to a server, wherein the watering quantity, the fertilizing amount and the fertilizing types form decision variables; building a complicated non-linear relationship between plant health indexes and all influence factor matrices X of plants in the server by an Elman neural network, and obtaining a plant culture model; optimizing the plant culture model by an NSGA-II algorithm, and obtaining a group of optimal solutions of the decision variables; using the group of optimal solutions of the decision variables as a plant recommendation decision X*, and issuing the recommendation decision to terminal equipment of a user through the server for displaying; culturing the plants by the user according to the recommendation decision displayed by the terminal equipment. By using the plant culture method and system provided by the invention, an optimal plant culture scheme can be determined; better living environment is created for the plants.

The invention belongs to the technical field of agricultural aquaculture, and particularly relates to a culture method for improving pond sediment by planting ryegrasses and breeding fowls. One culture cycle of the culture method comprises the following steps of: (1) culturing the ryegrasses at the bottom of a pond from the middle ten days of September to the end of next April; (2) breeding the fowls beside the pond from November to the middle ten days of the next April; (3) breeding fish fries in the pond from the middle ten days of the April to the end of May; and (4) culturing prawns from June to the middle ten days of the September. The culture method provides an ecological type comprehensive culture model, namely, during the idle period of a culture pond, the ryegrasses are planed to improve the pond sediment, are harvested to serve as a succulence of geese, and are used for feeding high-quality young geese in twice, so on the first hand, the breeding cost of the geese is reduced, and on the other hand, the pond sediment environment is improved, the cost for cleaning the pond is reduced under the action of a biological energy chain, and the culture method is favorable for high and sable yield of the aquaculture.

The invention discloses a multifunctional composite microecological preparation for sea cucumber cultivation, which comprises: Bacillus atrophaeus, Bacillus subtilis, Bacillus cereus, and a carrier. The invention screens good symbiosis between probiotics, has good antagonistic effect on sea cucumber pathogenic bacteria, and has the function of improving sea cucumber growth speed, immunity and intestinal digestive enzyme activity, and achieves antibacterial, Multiple functions of immunity and growth promotion. The indigenous bacteria screened from the intestines of sea cucumbers are safe and non-toxic to sea cucumbers, have a fast reproduction speed, are easy to cultivate, have strong adaptability to the environment, and can reproduce quickly to form dominant bacteria. The method of use is simple, and the preparation is only required to be added to the feed according to the ratio for feeding, and is suitable for young ginseng breeding and sea cucumber disease control in various breeding modes. It plays an important role in improving the quality of sea cucumber products and ensuring food safety.

The invention relates to lactobacillus plantarum Lp90, and a screening method and application thereof, in particular to lactobacillus plantarum with a function of inhibiting fat accumulation. The lactobacillus plantarum is named Lp90 and is preserved in the common microbiology center of the Chinese microbial strain preservation management committee, and the preservation number is CGMCC No.10453. The lactobacillus plantarum is obtained through an in-vitro nematode screening model, the fat accumulation of nematode can be reduced by 60 percent, and the lactobacillus plantarum has a potential function of preventing obesity and can be added into food, health care products or medicines as probiotics. Meanwhile, the invention provides the screening method namely a nematode culture model, and fat accumulation data of the nematode can be qualitatively and quantitatively detected, so that strains with the function of inhibiting the fat accumulation can be conveniently and quickly screened, the accuracy rate is high, and the economic cost is lower.

The invention discloses a high density culture method of stable and high output hair weed cell, which comprises the following steps: pre-culturing hair weed cell seed; adjusting the initial pH value of the culture medium in shake or biological reactor above 7. 0; switching in pre-culturing hair weed cell seed in the culture medium; ending the culture when the growth of hair weed cell enter late time of the stable period; collecting the hair weed cell and culture liquid. This invention adopts mixed nourishing or heterotrophy culture model, which lays the groundwork for endurance utilization of hair weed source.

The invention belongs to the field of cytobiology and specifically relates to an in vitro three-dimensional culture model for research on vasculogenic mimicry of glioma stem cells and an application thereof. Preparation of the in vitro three-dimensional culture model comprises the following steps: immersing a three-dimensional collagen scaffold in a DMEM culture medium for 6-24 h, taking out the three-dimensional collagen scaffold, removing superfluous DMEM adhered to the three-dimensional collagen scaffold to obtain a pretreated three-dimensional collagen scaffold, placing the pretreated three-dimensional collagen scaffold on a cell culture plate, culturing sphere-formed glioma stem cells in the pretreated three-dimensional collagen scaffold, standing at 37 DEG C for 2-6 h, adding an endothelial medium, and culturing at 37 DEG C for 2-4 days, so as to obtain the in vitro three-dimensional culture model for research on vasculogenic mimicry of glioma stem cells. Each operational process of the in vitro three-dimensional culture model is carried out at room temperature; various kinds of in situ staining, such as immunohistochemical staining and immunofluorescent staining, can be carried out; electron microscopic examination can be carried out; and RT-PCR and Western Blot detection can be carried out directly after digestion.

The invention relates to a tilapia and scylla serrata polyculture method which comprises the steps that a pond with unobstructed intake and drainage channels, matched electric power and complete oxygenation facilities is selected and disinfected thoroughly and wholly; then tilapia fry are put in; scylla serrata are put in after 30 days; tilapia complete formula feed is fed two times per day; water body disinfection is performed by quick lime every other 15-20 days in a culture process; water quality regulation is performed by a biological agent; the scylla serrata are subjected to crab pot catch rotation after cultured for 2-3 months; and the pond is dried, and tilapia is caught after cultured for 6 months. According to the method, a unique feasible technical operation route is adopted; the tilapia and the scylla serrata are collocated reasonably due to the life habit and food habit characteristics; a culture water environment is fully exerted; a bait utilization ratio is increased; a bottom environment is improved effectively; mass breeding of harmful microorganisms is reduced; the incidence is reduced; survival rates are increased obviously compared with monoculture; yield increasing and efficiency increasing are achieved; and the method is an efficient, environment-friendly and healthy culture model.

Several species of bacteria capable of invasive infections, such as S. pyogenes, S. equi and P. multocida, contain hyaluronic acid (HA) in their capsules. Bacterial species such as Staphylococcus aureus and related Staphylococci have capsules that contain acidic polysaccharides. Bacterial capsule or bacterial surface binding peptides were synthesized and tested in a culture model of invasive bacterial infections, specifically translocation through polarized keratinocyte cultures. The peptides reduced the translo-cation of a variety of bacterial species, with a concomitant increase in bacterial internalization by the keratinocytes. In vivo, subcutaneous inoculation of encapsulated GAS treated with peptides delayed bacterial dissemination. In a mouse surgical wound control model infected with S. aureus, treatment with peptides reduced the numbers of bacteria and inflammation at the wound site.

The invention discloses a single-layer or multi-layer 3D brain glioma cell culture model, and a construction method and an application thereof. The construction method includes the steps: freezing a decellularized brain tissue, and cut into slices; freeze-drying the slices, and sterilizing to obtain a scaffold; and inoculating a single-slice or multi-slice slice scaffold with brain glioma cells to obtain a single-layer or multi-layer brain glioma cell 3D culture model. The used decellularized brain slice scaffold for constructing the 3D brain glioma model has the advantages of complete removal of cells and fewer nucleic acid residues, can partially preserve a stereoreticular structure and biological components of an original brain matrix, is suitable for growth of the brain glioma cells, and can simulate a microenvironment of the growth of the brain glioma cells in vivo; the multi-layer 3D brain glioma cell culture model can also simulate the hypoxia and nutrition gradient of glioma in vivo, is more close to the brain glioma cells growing in vivo, and is suitable for screening of antitumor drugs, co-culture of the brain glioma cells and tumor-related cells, and study on the mechanism of action between the brain glioma cells and ECM.

The invention relates to an in-vitro model construction method for predicting toxicity of a compound upon fatty liver diseases. The method comprises the following steps: culturing different liver cells under a two-dimensional or three-dimensional condition, and culturing a fat converted liver cell in-vitro culture model by using a culture medium with a free fatty acid component. By adopting the in-vitro culture system, a growth micro environment in a liver cell can be simulated, the morphology and the function of the liver cell can be maintained for a long time, a cytochrome enzyme level is approximate to that in vivo, and the model has typical characteristics of fat converted liver cells, is capable of replacing fatty liver animal models, and achieving toxicology metabolism detection or pharmacodynamics study on a compound in long-term action on fat converted liver tissue in vitro.

The invention discloses a micro hepatic tissue culture model and a construction method and application thereof. The micro hepatic tissue culture model is characterized in that one or more of hepatic cells, hepatocyte-like cells and hepatic related cells are mixed with acellular hepatic bio-matrix scaffold LBS or LBS powder under conventional conditions to obtain hepatic cell spheroidal aggregate.The model herein can be utilized to reproduce complex biological and bio-chemical related micro environments, the micro environments closer to in-vivo cells are provided for hepatic cells or hepatocyte-like cells, the sensitivity of hepatic cells or hepatocyte-like cells to hepatotoxic compounds, and the model herein is applicable to the evaluation of hepatotoxic safety for medicine, Chinese herbs, compounds, cosmetics and other matters.

The present invention provides a simple and robust human liver cell-based system in which persistent hepatitis C infection, persistent hepatitis B infection or ethanol exposure induces a clinical Prognostic Liver Signature (PLS) high-risk gene signature. The cellular model system for hepatocellular carcinoma (HCC)/cirrhosis development and progression may be used in the screening of compounds useful in the treatment and/or prevention of cirrhosis and/or HCC as well as in the identification biomarkers for the prediction of liver disease (especially cirrhosis) progression and HCC. The present invention also relates to specific compounds that have been identified, using such screening methods, as useful in the treatment and/or the prevention of HCC/cirrhosis.

The invention provides a bacteria culture medium product containing a feather meal as a plant leftover. The bacteria culture medium product is developed by replacing peptone in an LB (Lysogeny Broth) culture medium by the feather meal as the plant leftover and can be used for effectively culturing model escherichia coli, bacillus subtilis, other gram-negative bacteria and other gram-positive bacteria by virtue of liquid shake flask fermentation, wherein the feather meal used by the product is prepared by the steps of carrying out acidolysis on feathers, extracting plant leftovers of a part of amino acid, and carrying out neutralization and concentration to obtain the feather meal. Whether partly or wholly replacing the peptone in the LB culture medium, the bacteria culture medium product can both be used for effectively carrying out liquid fermentation on a strain for test. Meanwhile, By virtue of adopting a novel feather meal culture medium, the biomasses of the model escherichia coli and the bacillus subtilis in a stable period can be remarkably increased.

The invention discloses a simulation three-dimensional incubator and a culture method for mouth mucosa epithelial cells and applications of the incubator and the culture method. The incubator comprises a single or a plurality of basic structural units, wherein each basic structural unit consists of an inner container and an outer container which are tightly nested with each other, and are prepared from an organic material for cell culture; and the bottom of the inner container consists of a permeable supporting film, and a certain gap is formed between the bottom of the inner container and the bottom of the outer container. The incubator takes the factors of nutrient supply of the mouth mucosa epithelial cells, blood vessels and blood vessel endothelial cells into account, and the growing environment of the mouth mucosa epithelial cells in the incubator is close to that in a human body, so that the incubator can be used for physiological, pharmacological, toxicological and micro-ecological studies of mouth mucosas, and can also be used for constructing human tissue engineering mouth mucosas and repairing and reconstructing mucosal coloboma. The new technology of the simulation three-dimensional incubator promotes an in vitro culture model of a mouth mucosa to a new height, so that related studies are closer to clinical practices.

The invention belongs to the field of biotechnology, relates to a method for stem cell culture and discloses application of VX-702 to preparation of a neural stem cell culture medium for promoting proliferation of the neural stem cells or maintaining dryness of the neural stem cells. The VX-702 can effectively increase the proliferation of the neural stem cells, promote formation of neural stem cell neurospheres, and significantly increase the expression of neural stem cell markers, such as NESTIN and SOX2. The invention also provides a method for promoting the proliferation of the neural stemcells. The method includes the steps of culturing the neural stem cells with the neural stem cell culture medium containing the VX-702 under the conditions of 35 -39 DEG C and 3%-8% of CO2 in an adherent culture mode. The VX-702 is used as an additive, the neural stem cell culture medium and the method for promoting the proliferation of the neural stem cells are provided, and building of a stableand reliable in-vitro culture model of the neural stem cells is facilitated.

The invention discloses a hybrid optimization method of three-level hybrid three-level optimization loach fingerlings. Related materials in steps comprise: A, tropic stock loach of two-year-old pond smelt of 50 Jin; B, cold zone stock loach of two-year-old female fish of 100 Jin; C, (1) sulfuretted hydrogen, (2) nitrite, (3) ammonia nitrogen, and (4) blue-green algae, and D, (1) fish chorionic gonadotrophin, and (2) domperidone injection liquid. The hybrid optimization method is a method of survival of the fittest and includes four steps. The hybrid optimization method of the three-level hybrid three-level optimization loach fingerlings can culture good stocks. Moreover, the stocks has strong vitality, disease-resistant capacity and anti-pollution capacity, diseases are few, medicine feeding is not needed during culture production, growth is fast, culture is easy, acre yield is high, male bodies and female bodies are uniform in size and good in quality, and the hybrid optimization method is suitable for an ecotype pollution-free high density culture model, can save culture sites, and is suitable for culturing the good stocks of export for earning foreign exchange.

The present invention relates to the use of an air-liquid interface culture model using asthmatic bronchial epithelial cells, which exhibit impaired epithelial barrier function. It is proposed to use asthmatic epithelial cultures, in the absence of added Th2 or proinflammatory cytokines such as IL-13, as an in vitro model to screen for agents that can act to improve impaired asthmatic epithelial barrier function. Such agents may have therapeutic utility in asthma patients.

The invention relates to the technical field of crab cultivation, in particular to a symbiotic culture method of rice and crabs. The symbiotic culture method includes: setting a temporary culture pond; treatment of the culture pond and temporary culture of the crabs; setting up of the rice field for culture; setting up escape prevention facility; rice planting and crab culture; crab and pond management, wherein the feeding method in the pond management includes: preparing fodder according to 3.5-4% of the weight of the crabs, releasing the fodder on a fodder table on a regular basis, checkingwhether the crabs are shelled or not every day, stopping feeding each time the crabs are shelled, increasing the amount of feed after shelling is completed, preparing the fodder according to 6-8% of the weight of the crabs when the crabs are shelled for the fourth time, and increasing the number of daily feed releases. The symbiotic culture method has the advantages that rice planting is combinedwith crab culture to form the symbiotic ecological culture model of rice and crabs, the dependence of rice on the use of chemical fertilizer is avoided, green food supply is formed, and the income offarmers can be greatly increased.