46 results about "Culture model" patented technology

Several species of bacteria capable of invasive infections, such as S. pyogenes, S. equi and P. multocida, contain hyaluronic acid (HA) in their capsules. Bacterial species such as Staphylococcus aureus and related Staphylococci have capsules that contain acidic polysaccharides. Bacterial capsule or bacterial surface binding peptides were synthesized and tested in a culture model of invasive bacterial infections, specifically translocation through polarized keratinocyte cultures. The peptides reduced the translocation of a variety of bacterial species, with a concomitant increase in bacterial internalization by the keratinocytes. In vivo, subcutaneous inoculation of encapsulated GAS treated with peptides delayed bacterial dissemination. In a mouse surgical wound model infected with S. aureus, treatment with peptides reduced the numbers of bacteria and inflammation at the wound site.

The invention relates to a hepatitis virus in-vitro culture model as well as a construction method and application thereof. The hepatitis virus in-vitro culture model is constructed by adopting human fetal liver stem cells and comprises the steps of carrying out separate culture on human fetal liver stem cell, immunohistochemically identifying human fetal liver stem cell and infecting human fetal liver stem cell with hepatitis virus by blood serum and verifying whether hepatitis virus infects the human fetal liver stem cells or not and the virus propagate and continuously secrete outside the cell or not; the model can be used for the screening and effect evaluation of an in-vitro hepatitis virus resistant medicament; the human fetal liver stem cells used in the hepatitis virus in-vitro culture model can be infected with hepatitis virus and can continuously secrete hepatitis virus; the human fetal liver stem cells can be subcultured; and the invention has simple and convenient method and high repeatability.

The invention relates to an assay for diagnosing a disease or affliction that affects fluid uptake or secretion or for studying the effectiveness of one or more drugs for treating the disease or affliction, wherein the assay comprises measuring swelling of one or more organoids.

The invention belongs to the field of cell biology, and in particular relates to an in vitro three-dimensional culture model for studying vascular mimicry of glioma stem cells and its application. The preparation of the in vitro three-dimensional culture model is carried out according to the following steps: the three-dimensional collagen scaffold is soaked in DMEM medium for 6-24 hours and then taken out, and the redundant DMEM adhered to the three-dimensional collagen scaffold is removed to obtain a pretreated three-dimensional collagen scaffold. Treat the three-dimensional collagen scaffold and place it in a cell culture plate, plant the glioma stem cells into the pretreated three-dimensional collagen scaffold, let it stand at 37°C for 2-6 hours, add endothelial medium, and culture at 37°C for 2-4 days , which is an in vitro three-dimensional culture model for studying vascular mimicry of glioma stem cells. All operations of this in vitro three-dimensional culture model are carried out at room temperature, and various stainings can be performed in situ, including immunohistochemistry and immunofluorescence staining; electron microscope detection is possible; RT‑PCR and Western Blot detection can be performed directly after digestion .

The invention relates to a tilapia and scylla serrata polyculture method which comprises the steps that a pond with unobstructed intake and drainage channels, matched electric power and complete oxygenation facilities is selected and disinfected thoroughly and wholly; then tilapia fry are put in; scylla serrata are put in after 30 days; tilapia complete formula feed is fed two times per day; water body disinfection is performed by quick lime every other 15-20 days in a culture process; water quality regulation is performed by a biological agent; the scylla serrata are subjected to crab pot catch rotation after cultured for 2-3 months; and the pond is dried, and tilapia is caught after cultured for 6 months. According to the method, a unique feasible technical operation route is adopted; the tilapia and the scylla serrata are collocated reasonably due to the life habit and food habit characteristics; a culture water environment is fully exerted; a bait utilization ratio is increased; a bottom environment is improved effectively; mass breeding of harmful microorganisms is reduced; the incidence is reduced; survival rates are increased obviously compared with monoculture; yield increasing and efficiency increasing are achieved; and the method is an efficient, environment-friendly and healthy culture model.