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46 results about "Culture model" patented technology
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A corporate culture model is the organizing principle behind the values, beliefs and attitudes that characterize a company and guide its practices. Corporate cultures are often categorized by the degree of emphasis placed on various defining elements such as hierarchy, process, innovation, collaboration, competition, community involvement and social engagement.
Web Enabled Three-Dimensional Visualization
InactiveUS20080231630A1Geographical information databasesSpecial data processing applicationsTerrainComputer graphics (images)
A method for presenting a perspective view of a real urban environment, augmented with associated geo-coded content, and presented on a display of a terminal device. The method comprises the steps of: connecting the terminal device to a server via a network; communicating user identification, user present-position information and at least one user command, from the terminal device to the server; processing a high-fidelity, large-scale, three-dimensional (3D) model of an urban environment, and associated geo-coded content by the server; communicating the 3D model and associated geo-coded content from said server to said terminal device, and processing said data layers and said associated geo-coded content, in the terminal device to form a perspective view of the real urban environment augmented with the associated geo-coded content. The 3D model comprises a data layer of 3D building models; a data layer of terrain skin model; and a data layer of 3D street-level-culture models. The processed data layers and the associated geo-coded content correspond to the user present-position, the user identification information, and the user command.
Owner:SHENKAR VICTOR +1
Feeding method of bait for breeding Hippocampus trimaculatus Leach
InactiveCN101248769AIncrease growth rateImprove survival rateClimate change adaptationAnimal feeding stuffLife habitCulture model
The invention discloses a feeding method of culturing the baby hippocampus trimaculatus leach. The technical method designs a screening and converting method of the fish bait type in different growth time suitable for the baby Hippocampus Trimaculatus Leach based on the baby's ingestion and life habits. The technical method changes the conventional culture model of the baby hippocampus trimaculatus leach and can remarkably increase the growth rate and the survival rate of the baby hippocampus trimaculatus leach.
Owner:李秉记
Plant culture method and system based on IoT (Internet of things) big data analysis
The invention provides a plant culture method and system based on IoT (Internet of things) big data analysis, wherein the method comprises the steps of collecting plant species, soil humidity, a soil pH value, illumination intensity, environment temperature, environment humidity, images, watering quantity, fertilizing amount and fertilizing types; forming influence factor matrices X; uploading the influence factor matrices X to a server, wherein the watering quantity, the fertilizing amount and the fertilizing types form decision variables; building a complicated non-linear relationship between plant health indexes and all influence factor matrices X of plants in the server by an Elman neural network, and obtaining a plant culture model; optimizing the plant culture model by an NSGA-II algorithm, and obtaining a group of optimal solutions of the decision variables; using the group of optimal solutions of the decision variables as a plant recommendation decision X*, and issuing the recommendation decision to terminal equipment of a user through the server for displaying; culturing the plants by the user according to the recommendation decision displayed by the terminal equipment. By using the plant culture method and system provided by the invention, an optimal plant culture scheme can be determined; better living environment is created for the plants.
Owner:重庆扩博科技有限公司
Culture method for improving pond sediment by planting ryegrasses and breeding fowls
InactiveCN101897268APromote healthy developmentReduce stable and high yieldClimate change adaptationExcrement fertilisersFowlPrawn
The invention belongs to the technical field of agricultural aquaculture, and particularly relates to a culture method for improving pond sediment by planting ryegrasses and breeding fowls. One culture cycle of the culture method comprises the following steps of: (1) culturing the ryegrasses at the bottom of a pond from the middle ten days of September to the end of next April; (2) breeding the fowls beside the pond from November to the middle ten days of the next April; (3) breeding fish fries in the pond from the middle ten days of the April to the end of May; and (4) culturing prawns from June to the middle ten days of the September. The culture method provides an ecological type comprehensive culture model, namely, during the idle period of a culture pond, the ryegrasses are planed to improve the pond sediment, are harvested to serve as a succulence of geese, and are used for feeding high-quality young geese in twice, so on the first hand, the breeding cost of the geese is reduced, and on the other hand, the pond sediment environment is improved, the cost for cleaning the pond is reduced under the action of a biological energy chain, and the culture method is favorable for high and sable yield of the aquaculture.
Owner:YUYAO AQUATIC PROD TECH POPULARIZATION CENT
Composite microecological preparation for multifunctional sea cucumber culture
InactiveCN102283328AEasy to useReduce usageAntibacterial agentsBacteriaGrowth promotingBacillus cereus
The invention discloses a multifunctional composite microecological preparation for sea cucumber cultivation, which comprises: Bacillus atrophaeus, Bacillus subtilis, Bacillus cereus, and a carrier. The invention screens good symbiosis between probiotics, has good antagonistic effect on sea cucumber pathogenic bacteria, and has the function of improving sea cucumber growth speed, immunity and intestinal digestive enzyme activity, and achieves antibacterial, Multiple functions of immunity and growth promotion. The indigenous bacteria screened from the intestines of sea cucumbers are safe and non-toxic to sea cucumbers, have a fast reproduction speed, are easy to cultivate, have strong adaptability to the environment, and can reproduce quickly to form dominant bacteria. The method of use is simple, and the preparation is only required to be added to the feed according to the ratio for feeding, and is suitable for young ginseng breeding and sea cucumber disease control in various breeding modes. It plays an important role in improving the quality of sea cucumber products and ensuring food safety.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Construction method and application of human normal vaginal epithelium 3D (Three Dimensional) differentiation culture model
The invention belongs to the field of biomedicines and discloses a construction method and application of a human normal vaginal epithelium 3D (Three Dimensional) differentiation culture model. A 2D (Two Dimensional) growth culture medium is provided and normal vaginal epithelial cells separated and cultured from normal tissues beside female vaginal cancer are obtained; any exogenous gene is not introduced and the normal vaginal epithelial cells have a physiological function of normal differentiation. The method for constructing the human normal differentiation vaginal epithelium 3D model comprises the following steps: re-suspending single cells by the 2D culture medium and inoculating the single cells into a gas-liquid culture device; replacing the growth culture medium with a differentiation culture medium and culturing for 14 to 21 days; after completely differentiating 3D gas-liquid culture of the human vaginal epithelial cells, inoculating HSV-2 (Herpes Simplex Virus-2) virus liquid to obtain an HSV-2 virus infection 3D model. The two types of 3D models can be used for physiological studies and drug toxicity safety evaluation of human normal reproductive tract epithelium, researches of pathogenic mechanisms of HSV-2 virus infected diseases and sexual transmission pathogen infected diseases and researches and development of anti-viruses medicines.
Owner:深圳涌泰生物科技有限公司
Lactobacillus plantarum Lp90, and screening method and application thereof
ActiveCN104928208AAvoid accumulationEasy to filterBacteriaMicrobiological testing/measurementMicroorganismNematode
The invention relates to lactobacillus plantarum Lp90, and a screening method and application thereof, in particular to lactobacillus plantarum with a function of inhibiting fat accumulation. The lactobacillus plantarum is named Lp90 and is preserved in the common microbiology center of the Chinese microbial strain preservation management committee, and the preservation number is CGMCC No.10453. The lactobacillus plantarum is obtained through an in-vitro nematode screening model, the fat accumulation of nematode can be reduced by 60 percent, and the lactobacillus plantarum has a potential function of preventing obesity and can be added into food, health care products or medicines as probiotics. Meanwhile, the invention provides the screening method namely a nematode culture model, and fat accumulation data of the nematode can be qualitatively and quantitatively detected, so that strains with the function of inhibiting the fat accumulation can be conveniently and quickly screened, the accuracy rate is high, and the economic cost is lower.
Owner:JIANGSU WECARE BIOTECHNOLOGY CO LTD
Method for black moss cell high-density culture of stable high-yield black moss polysaccharide
InactiveCN101063085AHigh densityShort training periodUnicellular algaeFermentationHigh densityMicrobiology
The invention discloses a high density culture method of stable and high output hair weed cell, which comprises the following steps: pre-culturing hair weed cell seed; adjusting the initial pH value of the culture medium in shake or biological reactor above 7. 0; switching in pre-culturing hair weed cell seed in the culture medium; ending the culture when the growth of hair weed cell enter late time of the stable period; collecting the hair weed cell and culture liquid. This invention adopts mixed nourishing or heterotrophy culture model, which lays the groundwork for endurance utilization of hair weed source.
Owner:TIANJIN UNIV OF SCI & TECH
Hyaluronic acid binding peptides enhance host defense against pathogenic bacteria
Several species of bacteria capable of invasive infections, such as S. pyogenes, S. equi and P. multocida, contain hyaluronic acid (HA) in their capsules. Bacterial species such as Staphylococcus aureus and related Staphylococci have capsules that contain acidic polysaccharides. Bacterial capsule or bacterial surface binding peptides were synthesized and tested in a culture model of invasive bacterial infections, specifically translocation through polarized keratinocyte cultures. The peptides reduced the translocation of a variety of bacterial species, with a concomitant increase in bacterial internalization by the keratinocytes. In vivo, subcutaneous inoculation of encapsulated GAS treated with peptides delayed bacterial dissemination. In a mouse surgical wound model infected with S. aureus, treatment with peptides reduced the numbers of bacteria and inflammation at the wound site.
Owner:CANGENE CORP
In vitro three-dimensional culture model of glioma stem cells and application thereof
ActiveCN104312975AEasy to operateTube-like structure is reliableBiological testingTumor/cancer cellsStainingWestern blot
The invention belongs to the field of cytobiology and specifically relates to an in vitro three-dimensional culture model for research on vasculogenic mimicry of glioma stem cells and an application thereof. Preparation of the in vitro three-dimensional culture model comprises the following steps: immersing a three-dimensional collagen scaffold in a DMEM culture medium for 6-24 h, taking out the three-dimensional collagen scaffold, removing superfluous DMEM adhered to the three-dimensional collagen scaffold to obtain a pretreated three-dimensional collagen scaffold, placing the pretreated three-dimensional collagen scaffold on a cell culture plate, culturing sphere-formed glioma stem cells in the pretreated three-dimensional collagen scaffold, standing at 37 DEG C for 2-6 h, adding an endothelial medium, and culturing at 37 DEG C for 2-4 days, so as to obtain the in vitro three-dimensional culture model for research on vasculogenic mimicry of glioma stem cells. Each operational process of the in vitro three-dimensional culture model is carried out at room temperature; various kinds of in situ staining, such as immunohistochemical staining and immunofluorescent staining, can be carried out; electron microscopic examination can be carried out; and RT-PCR and Western Blot detection can be carried out directly after digestion.
Owner:THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
Hepatitis virus in-vitro culture model as well as construction method and application thereof
InactiveCN101914489ASimple methodGood repeatabilitySsRNA viruses positive-senseMicrobiological testing/measurementLiver Stem CellHuman fetal
Owner:INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
A rapid quantitative assay to measure cftr function in a primary intestinal culture model
ActiveUS20150276719A2Rapid and simple quantitative assayEasy to detectCompound screeningApoptosis detectionSecreted substanceSecretion
Owner:KONINK NEDERLANDSE AKADE VAN WETENSCHAPPEN +1
Tilapia and scylla serrata polyculture method
InactiveCN103392653AIncrease profitReduced BloomingClimate change adaptationPisciculture and aquariaPolycultureWater quality
The invention relates to a tilapia and scylla serrata polyculture method which comprises the steps that a pond with unobstructed intake and drainage channels, matched electric power and complete oxygenation facilities is selected and disinfected thoroughly and wholly; then tilapia fry are put in; scylla serrata are put in after 30 days; tilapia complete formula feed is fed two times per day; water body disinfection is performed by quick lime every other 15-20 days in a culture process; water quality regulation is performed by a biological agent; the scylla serrata are subjected to crab pot catch rotation after cultured for 2-3 months; and the pond is dried, and tilapia is caught after cultured for 6 months. According to the method, a unique feasible technical operation route is adopted; the tilapia and the scylla serrata are collocated reasonably due to the life habit and food habit characteristics; a culture water environment is fully exerted; a bait utilization ratio is increased; a bottom environment is improved effectively; mass breeding of harmful microorganisms is reduced; the incidence is reduced; survival rates are increased obviously compared with monoculture; yield increasing and efficiency increasing are achieved; and the method is an efficient, environment-friendly and healthy culture model.
Owner:HAINAN ACADEMY OF OCEAN & FISHERIES SCI
Primary culture method in serum-free medium for fetal rat cortical neurons
The invention discloses a primary culture method in a serum-free medium for fetal rat cortical neurons. According to the method, an insulin-transferrin-sodium selenite serum-free medium is used to culture the fetal rat cortical neurons, and neuron-specific enolase is used for dyeing to identify the fetal rat cortical neurons. According to the primary culture method in a serum-free medium for the fetal rat cortical neurons, dissociated culture model of Wistar fetal rat cortical in vivo is established by using a serum-free culture method, and morphologic observation of the cultured neurons is carried out, establishing a foundation for relative researches in future.
Owner:SUZHOU HEALTH COLLEGE
Hyaluronic Acid Binding Peptides Enhance Host Defense Against Pathogenic Bacteria
ActiveUS20090030180A1Avoid infectionAvoid bacterial infectionAntibacterial agentsPeptide-nucleic acidsBacteroidesCulture model
Several species of bacteria capable of invasive infections, such as S. pyogenes, S. equi and P. multocida, contain hyaluronic acid (HA) in their capsules. Bacterial species such as Staphylococcus aureus and related Staphylococci have capsules that contain acidic polysaccharides. Bacterial capsule or bacterial surface binding peptides were synthesized and tested in a culture model of invasive bacterial infections, specifically translocation through polarized keratinocyte cultures. The peptides reduced the translo-cation of a variety of bacterial species, with a concomitant increase in bacterial internalization by the keratinocytes. In vivo, subcutaneous inoculation of encapsulated GAS treated with peptides delayed bacterial dissemination. In a mouse surgical wound control model infected with S. aureus, treatment with peptides reduced the numbers of bacteria and inflammation at the wound site.
Owner:CANGENE CORP
Single-layer or multi-layer 3D brain glioma cell culture model, and construction method and application thereof
ActiveCN110257335AGuaranteed uniformityEasy to observeCompound screeningApoptosis detectionFreeze-dryingOxygen
The invention discloses a single-layer or multi-layer 3D brain glioma cell culture model, and a construction method and an application thereof. The construction method includes the steps: freezing a decellularized brain tissue, and cut into slices; freeze-drying the slices, and sterilizing to obtain a scaffold; and inoculating a single-slice or multi-slice slice scaffold with brain glioma cells to obtain a single-layer or multi-layer brain glioma cell 3D culture model. The used decellularized brain slice scaffold for constructing the 3D brain glioma model has the advantages of complete removal of cells and fewer nucleic acid residues, can partially preserve a stereoreticular structure and biological components of an original brain matrix, is suitable for growth of the brain glioma cells, and can simulate a microenvironment of the growth of the brain glioma cells in vivo; the multi-layer 3D brain glioma cell culture model can also simulate the hypoxia and nutrition gradient of glioma in vivo, is more close to the brain glioma cells growing in vivo, and is suitable for screening of antitumor drugs, co-culture of the brain glioma cells and tumor-related cells, and study on the mechanism of action between the brain glioma cells and ECM.
Owner:BEIJING TIANTAN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV +1
Staphylococcus simulans L-RG18 fermentation medium for use and high density culture method
ActiveCN103710279AHigh viable countAvoid repeated washingBacteriaMicroorganism based processesHigh densityCoupling
The invention discloses a high density fermentation technology of staphylococcus simulans L-RG18 having good fermentation characteristics and separated from France dry sausage, the high density fermentation technology is a high density culture model comprising optimal control of a fermentation culture medium and fermentation process conditions and repeated in-batch coupling cell cycle, according to the high density fermentation technology, a high density fermentation liquid with the living bacterium number of 3.72*1011 CFU / mL is obtained, and the high density fermentation technology lays a foundation for further studies on preparation of direct-adding type staphylococcus simulans meat starter cultures.
Owner:CHINA AGRI UNIV
In-vitro model construction method for predicting toxicity of compound upon fatty liver diseases
InactiveCN109337863AHas adipocytic propertiesLower levelCompound screeningApoptosis detectionHepaticaEnzymes levels
The invention relates to an in-vitro model construction method for predicting toxicity of a compound upon fatty liver diseases. The method comprises the following steps: culturing different liver cells under a two-dimensional or three-dimensional condition, and culturing a fat converted liver cell in-vitro culture model by using a culture medium with a free fatty acid component. By adopting the in-vitro culture system, a growth micro environment in a liver cell can be simulated, the morphology and the function of the liver cell can be maintained for a long time, a cytochrome enzyme level is approximate to that in vivo, and the model has typical characteristics of fat converted liver cells, is capable of replacing fatty liver animal models, and achieving toxicology metabolism detection or pharmacodynamics study on a compound in long-term action on fat converted liver tissue in vitro.
Owner:NANJING DRUM TOWER HOSPITAL
Micro hepatic tissue culture model and construction method and application thereof
ActiveCN108753687AClosely connectedTo promote metabolismHepatocytesMicrobiological testing/measurementIn vivoCulture model
The invention discloses a micro hepatic tissue culture model and a construction method and application thereof. The micro hepatic tissue culture model is characterized in that one or more of hepatic cells, hepatocyte-like cells and hepatic related cells are mixed with acellular hepatic bio-matrix scaffold LBS or LBS powder under conventional conditions to obtain hepatic cell spheroidal aggregate.The model herein can be utilized to reproduce complex biological and bio-chemical related micro environments, the micro environments closer to in-vivo cells are provided for hepatic cells or hepatocyte-like cells, the sensitivity of hepatic cells or hepatocyte-like cells to hepatotoxic compounds, and the model herein is applicable to the evaluation of hepatotoxic safety for medicine, Chinese herbs, compounds, cosmetics and other matters.
Owner:ACADEMY OF MILITARY MEDICAL SCI
Method for inducing cell malignant transformation culture model
The invention relates to a method for inducing a cell malignant transformation culture model. The method comprises the following steps: (1) culturing immortalized human gastric mucosal epithelial GES-1 in a DMEM culture solution containing 10% fetal bovine serum to obtain cultured cells; (2) culturing Cag A-positive helicobacter pylori J99 in a brain heart infusion medium containing 10% of newborncalf serum and centrifuging to remove a supernatant to obtain cultured bacteria; (3) diluting the cultured bacteria with DMEM, adding the bacteria into the cultured cells for induction by inoculationevery other day, adding an MNNG solution daily, and obtaining an induced helicobacter pylori infection model 48 weeks later. The method is simple and convenient, not only various indexes of malignanttransformation of the cells can be observed, but also the cell model in which the helicobacter pylori and MNNG work together for long-term for malignant transformation of gastric mucosa can be established, and a good experiment foundation is laid for study of the subsequent gastric cancer formation mechanism and the development of cancer treatment drugs.
Owner:蔺莉
Clinical gene signature-based human cell culture model and uses thereof
ActiveUS20180119096A1Improved prognosisOrganic active ingredientsHepatocytesHepatocellular carcinomaCellular model
The present invention provides a simple and robust human liver cell-based system in which persistent hepatitis C infection, persistent hepatitis B infection or ethanol exposure induces a clinical Prognostic Liver Signature (PLS) high-risk gene signature. The cellular model system for hepatocellular carcinoma (HCC) / cirrhosis development and progression may be used in the screening of compounds useful in the treatment and / or prevention of cirrhosis and / or HCC as well as in the identification biomarkers for the prediction of liver disease (especially cirrhosis) progression and HCC. The present invention also relates to specific compounds that have been identified, using such screening methods, as useful in the treatment and / or the prevention of HCC / cirrhosis.
Owner:UNIVERSITY OF STRASBOURG +2
Bacteria culture medium product containing feather meal as plant leftover
The invention provides a bacteria culture medium product containing a feather meal as a plant leftover. The bacteria culture medium product is developed by replacing peptone in an LB (Lysogeny Broth) culture medium by the feather meal as the plant leftover and can be used for effectively culturing model escherichia coli, bacillus subtilis, other gram-negative bacteria and other gram-positive bacteria by virtue of liquid shake flask fermentation, wherein the feather meal used by the product is prepared by the steps of carrying out acidolysis on feathers, extracting plant leftovers of a part of amino acid, and carrying out neutralization and concentration to obtain the feather meal. Whether partly or wholly replacing the peptone in the LB culture medium, the bacteria culture medium product can both be used for effectively carrying out liquid fermentation on a strain for test. Meanwhile, By virtue of adopting a novel feather meal culture medium, the biomasses of the model escherichia coli and the bacillus subtilis in a stable period can be remarkably increased.
Owner:NANJING AGRICULTURAL UNIVERSITY +1
Simulation three-dimensional incubator and culture method for mouth mucosa epithelial cells and applications of incubator and culture method
InactiveCN103255058ATissue/virus culture apparatusEmbryonic cellsOral mucosal epithelial cellEcological study
The invention discloses a simulation three-dimensional incubator and a culture method for mouth mucosa epithelial cells and applications of the incubator and the culture method. The incubator comprises a single or a plurality of basic structural units, wherein each basic structural unit consists of an inner container and an outer container which are tightly nested with each other, and are prepared from an organic material for cell culture; and the bottom of the inner container consists of a permeable supporting film, and a certain gap is formed between the bottom of the inner container and the bottom of the outer container. The incubator takes the factors of nutrient supply of the mouth mucosa epithelial cells, blood vessels and blood vessel endothelial cells into account, and the growing environment of the mouth mucosa epithelial cells in the incubator is close to that in a human body, so that the incubator can be used for physiological, pharmacological, toxicological and micro-ecological studies of mouth mucosas, and can also be used for constructing human tissue engineering mouth mucosas and repairing and reconstructing mucosal coloboma. The new technology of the simulation three-dimensional incubator promotes an in vitro culture model of a mouth mucosa to a new height, so that related studies are closer to clinical practices.
Owner:GUANGDONG UNIV OF TECH
A three-dimensional culture model and application of glioma stem cells in vitro
ActiveCN104312975BEasy to operateTube-like structure is reliableBiological testingTumor/cancer cellsStainingImmunofluorescence staining
Owner:THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
Method for establishing in-vitro culture model of cryptosporidium baileyi
The invention discloses a method for establishing an in-vitro culture model of cryptosporidium baileyi. The method comprises the following steps of: purifying cryptosporidium baileyi oocysts, and then carrying out excystation on the cryptosporidium baileyi oocysts at 37-40 DEG C; then innocualting mixture of 5*10<4>-5*10<5> C. baileyi oocysts and sporozoites or 5*10<5>-2*10<6> purified sporozoites into embryonated embryo trachea tissues, and culturing at 37-40 DEG C. According to the method provided by the invention, the growth of the chicken-original cryptosporidium baileyi is researched by using an embryonated embryo trachea tissue culture model; the history of the C. baileyi part is completed in the system; and a new in-vitro C. baileyi culture model is established. The model. can be used for carrying out polypide growth analysis, toxicity test, internal and external gene expression, drug screening and the like.
Owner:HENAN AGRICULTURAL UNIVERSITY
Method for promoting proliferation of neural stem cells
InactiveCN109370989APromote proliferationNervous system cellsCell culture active agentsSOX2Stem cell culture
The invention belongs to the field of biotechnology, relates to a method for stem cell culture and discloses application of VX-702 to preparation of a neural stem cell culture medium for promoting proliferation of the neural stem cells or maintaining dryness of the neural stem cells. The VX-702 can effectively increase the proliferation of the neural stem cells, promote formation of neural stem cell neurospheres, and significantly increase the expression of neural stem cell markers, such as NESTIN and SOX2. The invention also provides a method for promoting the proliferation of the neural stemcells. The method includes the steps of culturing the neural stem cells with the neural stem cell culture medium containing the VX-702 under the conditions of 35 -39 DEG C and 3%-8% of CO2 in an adherent culture mode. The VX-702 is used as an additive, the neural stem cell culture medium and the method for promoting the proliferation of the neural stem cells are provided, and building of a stableand reliable in-vitro culture model of the neural stem cells is facilitated.
Owner:赛璟生物医药科技(上海)有限公司
Tilapia and scylla serrata polyculture method
InactiveCN103392653BIncrease profitReduced BloomingClimate change adaptationPisciculture and aquariaPolycultureWater quality
The invention relates to a tilapia and scylla serrata polyculture method which comprises the steps that a pond with unobstructed intake and drainage channels, matched electric power and complete oxygenation facilities is selected and disinfected thoroughly and wholly; then tilapia fry are put in; scylla serrata are put in after 30 days; tilapia complete formula feed is fed two times per day; water body disinfection is performed by quick lime every other 15-20 days in a culture process; water quality regulation is performed by a biological agent; the scylla serrata are subjected to crab pot catch rotation after cultured for 2-3 months; and the pond is dried, and tilapia is caught after cultured for 6 months. According to the method, a unique feasible technical operation route is adopted; the tilapia and the scylla serrata are collocated reasonably due to the life habit and food habit characteristics; a culture water environment is fully exerted; a bait utilization ratio is increased; a bottom environment is improved effectively; mass breeding of harmful microorganisms is reduced; the incidence is reduced; survival rates are increased obviously compared with monoculture; yield increasing and efficiency increasing are achieved; and the method is an efficient, environment-friendly and healthy culture model.
Owner:HAINAN ACADEMY OF OCEAN & FISHERIES SCI
Hybrid optimization method of three-level hybrid three-level optimization loach fingerlings
ActiveCN103081833AIncrease vitalityStrong disease resistanceClimate change adaptationPisciculture and aquariaThree levelDisease
The invention discloses a hybrid optimization method of three-level hybrid three-level optimization loach fingerlings. Related materials in steps comprise: A, tropic stock loach of two-year-old pond smelt of 50 Jin; B, cold zone stock loach of two-year-old female fish of 100 Jin; C, (1) sulfuretted hydrogen, (2) nitrite, (3) ammonia nitrogen, and (4) blue-green algae, and D, (1) fish chorionic gonadotrophin, and (2) domperidone injection liquid. The hybrid optimization method is a method of survival of the fittest and includes four steps. The hybrid optimization method of the three-level hybrid three-level optimization loach fingerlings can culture good stocks. Moreover, the stocks has strong vitality, disease-resistant capacity and anti-pollution capacity, diseases are few, medicine feeding is not needed during culture production, growth is fast, culture is easy, acre yield is high, male bodies and female bodies are uniform in size and good in quality, and the hybrid optimization method is suitable for an ecotype pollution-free high density culture model, can save culture sites, and is suitable for culturing the good stocks of export for earning foreign exchange.
Owner:陈家珍
Screening assay for improvement of epithelial barrier function
InactiveUS20060269909A1Improves epithelial barrier functionPlateau in measured barrier functionMicrobiological testing/measurementMaterial analysisAir liquid interfaceProinflammatory cytokine
The present invention relates to the use of an air-liquid interface culture model using asthmatic bronchial epithelial cells, which exhibit impaired epithelial barrier function. It is proposed to use asthmatic epithelial cultures, in the absence of added Th2 or proinflammatory cytokines such as IL-13, as an in vitro model to screen for agents that can act to improve impaired asthmatic epithelial barrier function. Such agents may have therapeutic utility in asthma patients.
Owner:UNIV OF SOUTHAMPTON
Symbiotic culture method of rice and crabs
InactiveCN110352885AAvoid relying onIncrease incomeClimate change adaptationAnimal feeding stuffFisheryPaddy field
The invention relates to the technical field of crab cultivation, in particular to a symbiotic culture method of rice and crabs. The symbiotic culture method includes: setting a temporary culture pond; treatment of the culture pond and temporary culture of the crabs; setting up of the rice field for culture; setting up escape prevention facility; rice planting and crab culture; crab and pond management, wherein the feeding method in the pond management includes: preparing fodder according to 3.5-4% of the weight of the crabs, releasing the fodder on a fodder table on a regular basis, checkingwhether the crabs are shelled or not every day, stopping feeding each time the crabs are shelled, increasing the amount of feed after shelling is completed, preparing the fodder according to 6-8% of the weight of the crabs when the crabs are shelled for the fourth time, and increasing the number of daily feed releases. The symbiotic culture method has the advantages that rice planting is combinedwith crab culture to form the symbiotic ecological culture model of rice and crabs, the dependence of rice on the use of chemical fertilizer is avoided, green food supply is formed, and the income offarmers can be greatly increased.
Owner:澧县湘琼大闸蟹养殖专业合作社
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