Composite microecological preparation for multifunctional sea cucumber culture

A compound micro-ecological and multi-functional technology, which is applied in the field of multi-functional compound micro-ecological preparations for sea cucumber cultivation, to achieve the effects of ensuring food safety, improving the quality of sea cucumber products, and reducing drug residues

Inactive Publication Date: 2011-12-21
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Before the present invention, there were many reports about probiotics for aquatic animals, such as: "aquaculture probiotics" such as Quan Min (patent application number: 200610042574.0), Wang Lianmin, etc. "probiotic co

Method used

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  • Composite microecological preparation for multifunctional sea cucumber culture
  • Composite microecological preparation for multifunctional sea cucumber culture
  • Composite microecological preparation for multifunctional sea cucumber culture

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Screening and safety experiments of potential probiotics

[0031] Take wild and healthy sea cucumbers, perform vivisection on sea cucumbers under aseptic conditions, take their intestines, squeeze out the contents of the intestines, wash the intestines with sterilized 15‰ normal saline three times, and homogenize the washed intestines , after equal proportion dilution, smear on TSB medium and 2216E seawater agar medium, select appropriate dilution plates to isolate bacteria, and obtain 240 strains of bacteria in total. 240 strains of purely cultured bacteria were cultured with casein hydrolysis medium and starch hydrolysis medium to measure their enzyme production activity. and Aeromonas hydrophlia (Aeromonas hydrophlia) as indicator bacteria, using spot method, cross method and Oxford cup method to detect the antibacterial effect of isolated bacteria on sea cucumber pathogenic bacteria, and screened out 3 strains with good antibacterial effect and Enzyme-producing bac...

Embodiment 2

[0036] Preparation of three active probiotic preparations

[0037] The preparation method of Bacillus atrophaeus CCTCC M 2010316 bacteria powder, Bacillus subtilis CCTCC M 2010314 bacteria powder, and Bacillus cereus CCTCC M 2010315 bacteria powder comprises the following steps:

[0038] A. Plate culture rejuvenation: Inoculate Bacillus atrophaeus, Bacillus subtilis, and Bacillus cereus stored at -80°C on TSB plate medium respectively, and cultivate them at 28°C for 24 hours to rejuvenate the strains and form a single colony;

[0039] B. Preparation of seed liquid: the bacterial classification cultivated in step A is made into 1×10 with 15‰ sterilized physiological saline 5 Inoculate the bacterial suspension of CFU / mL into a 250mL Erlenmeyer flask filled with 100mL of sterile TSB liquid medium at an inoculum size of 5%, and culture at 28°C and 170rpm for 18-20h with shaking;

[0040] C. Fermentation culture: Inoculate the seed solution in step B with 5% inoculum in a 5L Erlen...

Embodiment 3

[0044] Preparation of multi-functional compound micro-ecological preparation for sea cucumber cultivation: Compatibility with Bacillus atrophaeus CCTCC M2010316 bacteria powder, Bacillus subtilis CCTCC M 2010314 bacteria powder, and Bacillus cereus CCTCCM 2010315 bacteria powder according to the weight ratio of 1:1:1 , and mix well to prepare a compound microecological preparation.

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Abstract

The invention discloses a multifunctional composite microecological preparation for sea cucumber cultivation, which comprises: Bacillus atrophaeus, Bacillus subtilis, Bacillus cereus, and a carrier. The invention screens good symbiosis between probiotics, has good antagonistic effect on sea cucumber pathogenic bacteria, and has the function of improving sea cucumber growth speed, immunity and intestinal digestive enzyme activity, and achieves antibacterial, Multiple functions of immunity and growth promotion. The indigenous bacteria screened from the intestines of sea cucumbers are safe and non-toxic to sea cucumbers, have a fast reproduction speed, are easy to cultivate, have strong adaptability to the environment, and can reproduce quickly to form dominant bacteria. The method of use is simple, and the preparation is only required to be added to the feed according to the ratio for feeding, and is suitable for young ginseng breeding and sea cucumber disease control in various breeding modes. It plays an important role in improving the quality of sea cucumber products and ensuring food safety.

Description

technical field [0001] The invention relates to the technical field of microbial preparations, in particular to a multifunctional compound microecological preparation for sea cucumber cultivation which contains three kinds of probiotics and has multiple functions such as antibacterial, immune and growth promotion. Background technique [0002] Intestinal probiotics are a live bacterial preparation that mostly originates from the digestive tract and acts on the digestive tract. It can effectively supplement the beneficial microorganisms in the digestive tract of animals, inhibit the growth of pathogenic bacteria, improve the balance of digestive tract flora, and improve the immune function of aquatic animals. or growth rate. [0003] Sea cucumber (Apostichopus japonicus Liao, also known as sea cucumber), belongs to invertebrates, Echinodermata, sea cucumber class, and sea cucumber genus. Because of its high nutritional value, the scale of cultivation continues to expand, and...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/16A61K35/74A61K47/02A61P1/00A61P31/04A61P37/04C12N1/20C12R1/07C12R1/125C12R1/085A23K10/18A23K50/80A61K35/742
Inventor 王印庚张正廖梅杰李彬荣小军薛太山梁友王岚陈霞
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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