The invention provides a method for quickly constructing a trace
peptide base and a synthetizing device for realizing the method, belongs to the field of polypeptide synthesis, and solves the problemthat
a peptide base constructed by a conventional
SPOT method is low in efficiency. The method for quickly constructing the trace
peptide base comprises the following steps of S01: performing surfacederivatization on a lamellar
solid phase carrier; S02:
processing the lamellar
solid phase carrier into a plurality of structure units; S03: filling the structure units into a reaction tank; S04: adding
coupling reaction fluid to the reaction tank, performing a
coupling reaction, after the reaction, directly adding a cleaning
solvent to clean the reaction tank, after cleaning is completed, addingdeprotection reaction fluid to the reaction tank, performing a deprotection reaction, and after the reaction, directly adding the cleaning
solvent to clean the reaction tank; and S05: repeating the above step S04,
coupling amino acids one by one, selecting
coupling reaction fluid corresponding to each
amino acid during coupling different amino acids, and completing a lamellar
solid phase carrier loaded full-protection
peptide chain; and performing
pyrolysis to obtain naked peptides or loaded naked peptides. The method disclosed by the invention has the advantages of being high in peptide baseconstruction efficiency and the like.