34 results about "Spot method" patented technology

A spotting device for manufacturing a DNA microarray and a spotting method using the same are provided. The spotting device for dropping and immobilizing a solution of biomolecules, for example nucleic acids such as probe DNA, mRNA, and peptide nucleic acid (PNA), and proteins on a DNA microarray surface to manufacture a DNA microarray, includes a first microchannel with a tube shape; a supplying unit supplying the solution of biomolecules to the first microchannel; a biomolecule solution droplet forming unit cross-linked to the first microchannel and forming biomolecule solution droplets with a predetermined size by periodically jetting a gas toward the biomolecule solution flowing in the first microchannel; a second microchannel linked to the first microchannel and having a greater diameter than the first microchannel; a cooling unit surrounding at least a part of the second microchannel to freeze the biomolecule solution droplets which pass through the second microchannel; and a spotting unit thawing the frozen biomolecule solution droplets and dropping the thawed biomolecule solution droplets on a surface of the DNA microarray. The spotting device can form spots with uniform shape, minimize an effect of temperature on biomolecules, and easily manipulate biomolecules when manufacturing a DNA microarray.

Methods and systems selectively irradiate structures on or within a semiconductor substrate using multiple laser beams. The structures may be laser-severable conductive links, and the purpose of the irradiation may be to sever selected links.

Methods and systems selectively irradiate structures on or within a semiconductor wafer using multiple laser beams. The structures may be laser-severable conductive links, and the purpose of the irradiation may be to sever selected links. The structures are arranged in rows and may be processed in either an on-axis mode or a cross-axis mode. In the on-axis mode, the beam spots fall on structures in the same row as they move along the row. In the cross-axis mode, the beam spots fall on structures in different rows as they move along the rows.

The invention provides a fluorescent powder prefabricated film for a white light LED and a preparation method thereof. The components of the film are fluorescent powder and a binding agent, and the binding agent is silica gel or epoxide resin. The preparation method comprises the following steps: weighing the fluorescent powder and the binding agent according to mass ratio of the fluorescent powder and the binding agent being 1:0.5-5, and mixing the two uniformly; coating the mixture on a macromolecular material carrier by a silk-screen printing method to form a layer of film; and then curing for 10 to 300 minutes at a temperature of between 100 and 200 DEG C to prepare the film. A single LED packaged by the film can solve the problem of poor light-emitting consistency in the same batch and different batches in a glue spotting method, and saves a sorting process in the glue spotting method; and the film is adopted to directly manufacture a whole LED, and the production efficiency can be improved and the service life can be prolonged greatly.

The invention relates to an atmospheric correction and regional mineral map spotting method utilizing multi-scene ASTER (Advanced Spaceborne Thermal Emission and Reflection Radiometer) remote sensing data, comprising the following nine steps of: (1) reading in ASTER data; (2) selecting reference data, wherein one-scene data is selected from multi-scene data as a reference for atmospheric correction; (3) carrying out the atmospheric correction on the reference data to obtain reference reflectivity data; (4) carrying out atmospheric correction on non-reference data to obtain non-reference reflectivity data; (5) calculating the overlay region spectral characteristic difference of the reference reflectivity data and the non-reference reflectivity data; (6) calculating the atmospheric water vapor content and the atmospheric visibility of the non-reference data; (7) newly carrying out the atmospheric correction on the non-reference data by utilizing the calculated atmospheric water vapor content and the atmospheric visibility to obtain non-reference reflectivity data; (8) carrying out inlaying treatment on the multi-scene reflectivity data including the reference data and the non-reference data; and (9) carrying out mineral identification and map spotting by utilizing spectral indexes. The method effectively eliminates inlaid gaps among the multi-scene data, thereby realizing the regional mineral map spotting.

Methods and systems selectively irradiate structures on or within a semiconductor substrate using multiple laser beams. The structures may be laser-severable conductive links, and the purpose of the irradiation may be to sever selected links.

The invention relates to an electron beam surface treatment method, in particular to homogeneous beam spot technology in electron beam surface treatment technology, belonging to the technical field of surface treatment. The homogeneous beam spot technology for electron beam surface treatment mainly comprises the steps of beam spot waveform design, programming and process test and optimization, etc. The technology is characterized by designing the homogeneous beam spot waveform in conformity with the requirement of surface treatment according to the electron beam scanning principle and the energy distribution law in the movement process, carrying out curve fitting on the beam spot waveform by the least square method in a Cartesian coordinate system, programming the fitting result and storing the programmed fitting result into a computer control system, carrying out test and optimization by controlling the process parameters of electron beam surface treatment and finally obtaining large-area homogeneous beam spot technology. The method is easier to realize, has better economical efficiency, overcomes the defect that the energy of other electro beam spots are not distributed homogeneously, dispenses with extra control equipment and can be directly realized on the general electron beam equipment.

Method and apparatus for determining the location at which radiation is incident on the sensor system which includes an EMI screening mesh by comparing the actual image detected, which includes the higher order diffraction effects, with a search or sample image applied sequentially at different locations and determing the location of the sample image which gives the closest match. The sample image corresponds to an expected pattern or image from a point source incident at a particular location on a particular window/sensor system, and may be generated from a real source of known location and properties incident on the system, or may be a generated image, such as a standard image such as a cross pattern or determined mathematically, for example using Fourier transforms. Preferably, once the location of the point of incidence of the radiation has been determined, image processing techniques are applied to remove the diffraction spots.

The invention discloses an in-use vehicle engine oil dilution quick evaluation method and a testing device thereof. The method includes: using a measuring cylinder to sectionally receive in-use vehicle engine oil of V0 in total volume; dividing V1 of liquid obtained by condensing by V0 to get dilution R of detected engine oil, namely R=V1/V0, wherein V1 refers to volume after heating and condensing, R refers to the dilution of the detected engine oil, and the larger R is, the higher the dilution is. By the method, the problem that a gas chromatograph which is high in price needs to be arranged when existing chromatography is used to detect dilution of engine oil is solved, the problem that a spot method is low in accuracy and poor in repeatability is solved, and the problem that methods for quickly detecting engine oil dilution proportion in existing patents are long in titration time, high in calculation difficulty and low in pertinence is solved.

The invention provides a pressure casting die parting surface spotting method. The method is characterized by comprising the steps as follows: a molding lining mold and a slider of a pressure casting die are spotted preliminarily, and the contact area is required to exceed 70% of the area of parting surfaces in the natural state or under the smaller die clamping force; then, the molding lining die and the slider are assembled in a die frame for overall fine spotting of the die. The matching accuracy of the parting surfaces of the pressure casting die can be improved and the grinding efficiency is improved with the adoption of the method. Copper sheets in the same dimensions are uniformly distributed on the parting surfaces in the die spotting process, and the parallelism of the parting surfaces of moving and static dies is guaranteed. Finally, the contact area reaches 95% of the area of the parting surfaces or above after die matching, and the good spotting effect is realized.

The invention relates to a gold marking spotting method quantification detection equipment based on digital image processing, which is detection equipment based on a digital signal processor (DSP) and image processing technology, wherein a reaction plate image reading unit takes charge of reading reaction plate image, digital signals of the image are introduced to DSP, processed by the image process unit, corresponding concentration information to be measured is obtained, finally the information is output through a display unit. The invention is used in fast measurement of hormone content in humoral immunity of human body, concentration change of specific albumen, presence of pathogen or corresponding antibody, users can conveniently obtain concentration information of certain component to be measured in body fluid of certain human to be measured through simple operation, thereby delivering correct survey report to the doctor before the doctor makes decision, causing the most effective treatment on each specific patient.

The invention discloses a one-spot method for converting lutein ester into zeaxanthin, which is characterized by comprising the following steps of: (1) under the protection of nitrogen, heating a solvent to the temperature of between 50 and 80DEG C, adding a lutein extract into the solvent in a mass/volume ratio of 1:(5-20), adding alkali in a mass ratio of the lutein extract to the alkali of 1:(1-6), heating to the temperature of between 80 and 150DEG C, and reacting by keeping the temperature for 10 to 72 hours until the reaction is finished; and (2) adding water to stop the reaction, adding 5 to 45 volume percent acid to neutralize until the pH value is 4-7, filtering, washing, and performing vacuum drying at the temperature of between 30 and 50DEG C to obtain high-purity zeaxanthin. In the method, the lutein extract is not required to be saponified, the steps are simple and short, the operation is simple, the yield is high, and the used organic solvent and reagents have low toxicity or no toxicity. The prepared zeaxanthin can be further purified to form a zeaxanthin monomer and used for preparing zeaxanthin microcapsules.

A daily monitoring method for an ion implantation dip angle comprises the following steps that firstly, original data about the relation between a square resistor and the ion implantation dip angle are obtained; secondly, a monitoring sheet is selected, ion implantation with the corner angle being zero degree and the dip angle being zero degree is carried out, annealing is carried out after implantation is carried out, the resistance of the square resistor is measured, continuous weekly monitoring is carried out, and the square resistance statistical value is obtained; thirdly, the resistance statistical value obtained through monitoring is compared with the minimum square resistor resistance value in the original data in the first step, if the comparison result is in the difference range allowed by a system, normal production is carried out, and if the comparison result is not in the difference range allowed by the system, a fourth step is carried out; fourthly, the implantation dip angle is re-checked through a five-spot method. By means of the daily monitoring method, a small number of monitoring sheets can be used, the effect of continuously and weekly monitoring the implantation angle of an ion implanter for a long time is achieved, the material resources are saved, and the production cost is reduced.

The invention provides a one-pot method for synthesizing N-methylnitrone compounds, comprising the steps of: reducing nitromethane at first with a metal reducing agent, adding aromatic aldehyde directly into a reaction solution for heated reflux to obtain a target product having high yield. The invention uses the nitromethane and the aromatic aldehyde as raw materials, takes organometallic reagents such as zinc, tin and the like as the reducing agent, and employs a one-spot method for the heated reflux to directly synthesize the N-methylnitrone compounds. The method provided by the invention has the characteristics of less synthesis procedures, simple technique, easy operation, easily-available and inexpensive raw materials, mild reaction conditions, low synthesis cost, good product quality and high yield, as well as is an efficient and convenient method for synthesizing the N-methylnitrone compounds.

The invention provides a method for quickly constructing a trace peptide base and a synthetizing device for realizing the method, belongs to the field of polypeptide synthesis, and solves the problemthat a peptide base constructed by a conventional SPOT method is low in efficiency. The method for quickly constructing the trace peptide base comprises the following steps of S01: performing surfacederivatization on a lamellar solid phase carrier; S02: processing the lamellar solid phase carrier into a plurality of structure units; S03: filling the structure units into a reaction tank; S04: adding coupling reaction fluid to the reaction tank, performing a coupling reaction, after the reaction, directly adding a cleaning solvent to clean the reaction tank, after cleaning is completed, addingdeprotection reaction fluid to the reaction tank, performing a deprotection reaction, and after the reaction, directly adding the cleaning solvent to clean the reaction tank; and S05: repeating the above step S04, coupling amino acids one by one, selecting coupling reaction fluid corresponding to each amino acid during coupling different amino acids, and completing a lamellar solid phase carrier loaded full-protection peptide chain; and performing pyrolysis to obtain naked peptides or loaded naked peptides. The method disclosed by the invention has the advantages of being high in peptide baseconstruction efficiency and the like.