Detection method of medicine and toxin content in plasma preparation card
A technology for plasma and poisons, which is applied in the field of detection of drug and poison content in plasma preparation cards, can solve the problems of instability and inability to detect stable analytes in whole blood samples, and achieve easy transportation, high sensitivity and resolution, and detection The results are accurate
Pending Publication Date: 2018-09-28
李水军 +2
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Problems solved by technology
[0010] In order to overcome the technical problem of being unable to quickly, accurately and stably detect the concentration of common drugs or poisons in blood in the prior art, the present invention provides a method for detecting the content of drugs and poisons in plasma preparation cards
This method can solve the transportation of whole blood samples and the instability of analytes, and realize the accurate quantification of drugs and poisons in plasma samples from different sources
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Embodiment 1
[0063] The method of the invention adopts high performance liquid chromatography-tandem mass spectrometry detection technology to detect suspicious drugs or poisons in clinical suspected poisoning samples. Add an appropriate amount of extractant and internal standard working solution to the plasma preparation card sample collected clinically, and after shaking and centrifuging, take the supernatant and inject it into the sample for analysis. After the processed samples are chromatographically separated, they are detected by a quadrupole time-of-flight mass spectrometer full-scan trigger information-dependent retrieval (IDA) method, and are screened for matching degree with the information in the established preset analyte database , to determine the type of drug or poison; the multiple reaction monitoring mode (MRM) of triple quadrupole mass spectrometry is used to determine the concentration of suspected drug or poison in the plasma sample according to the parent ion and chara...
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Abstract
The invention discloses a method for detecting the content of at least one kind of target medicine or toxin in a plasma preparation card. The method comprises the following steps of after a plasma preparation card sample obtained in clinics is extracted according to certain steps, performing analysis by an ultrahigh-performance liquid chromatograph-quadrupole rod flight time mass spectrograph; obtaining the remaining time of the target medicine or toxin and high-resolution first-stage and second-stage mass-spectrogram; performing matching with the preset analysis article database; confirming the types of medicine or toxin; for medicine and toxin which are positive in screening, building the standard curve range applicable to clinic detection; quantitatively detecting the concentration of medicine or toxin in the plasma preparation card by using an ultrahigh-performance liquid chromatograph-triple quadrupole rod mass spectrograph technology. After the concentration is converted througha formula, the corresponding plasma concentration is obtained; the disease auxiliary diagnosis is provided for clinic doctors. Compared with a whole blood spot method, the detection method provided bythe invention has the advantages that the interference of the whole blood substrates and the blood cell hematocrit influence are eliminated; the detection result is more accurate; higher sensitivityand resolution ratio are realized.
Description
technical field [0001] The invention relates to a method for detecting compounds, in particular to a method for detecting the contents of drugs and poisons in plasma preparation cards. Background technique [0002] In recent decades, the annual poisoning of drugs, pesticides, inorganic substances, heavy metals and other toxic substances has been on the rise worldwide, and the number of poisoning deaths has continued to increase. According to the results of the third national cause of death survey conducted by the former Ministry of Health of my country, injury and poisoning are the fifth leading cause of death among Chinese residents, accounting for 10.7% of the total death toll, and occupational poisoning patients alone amounted to 3.7 million. The rate of million people is increasing. Common poisoning substances in emergency poisoning cases in my country include drugs, pesticides, rat poison, drugs, carbon monoxide, alcohol, heavy metals, etc. Among them, drug poisoning is...
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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8822
Inventor 李水军陆优丽姜凤丽刘朝超
Owner 李水军
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