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Preparation and application of 4′,5,7-trihydroxyflavone from Artemisia annua

A technology of trihydroxyflavone and Artemisia annua, applied in the field of medicine, can solve the problems of inconvenient extraction and preparation of active components of Artemisia annua, and achieve the effects of remarkable activity, simple and quick operation, and clear research goals.

Active Publication Date: 2018-05-15
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method is aimed at the preparation of the total flavonoids of Artemisia annua, which is not convenient for the extraction and preparation of the monomeric active ingredients in Artemisia annua, so the invention is of great significance to the preparation method of the monomeric active ingredients in Artemisia annua

Method used

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  • Preparation and application of 4′,5,7-trihydroxyflavone from Artemisia annua
  • Preparation and application of 4′,5,7-trihydroxyflavone from Artemisia annua
  • Preparation and application of 4′,5,7-trihydroxyflavone from Artemisia annua

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Embodiment 1

[0028] Example 1: Preparation method of Artemisia annua extract 4',5,7-trihydroxyflavone (pigenin)

[0029] 40kg dried Artemisia annua original medicinal material (purchased from Bozhou Chinese Medicinal Herbal Pieces Company, place of origin: Lin’an, Zhejiang, also known as Nan Liu Jinu), crushed, soaked in 80% ethanol, extracted by percolation at 65°C, and used successively 80% Ethanol 200L, thin film evaporated after extraction, dried for later use.

[0030] Take about 1kg of dry powder of 80% ethanol extract, soak it in 6L petroleum ether for 24h and filter it, dry the filter residue, soak it in 6L ethyl acetate for 24h and filter it, and concentrate the filtrate to get 6.2kg of ethyl acetate extract. The crude ethyl acetate extract was dissolved in methanol and applied to a silica gel column, and the petroleum ether-ethyl acetate system was used to: 10:1, 5:1, 2:1, 1:1, 1:2, 1:5, 1: 10 Each configuration was 400ml and eluted with a 2d / s (drop / second) gradient, and sample...

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Abstract

The invention provides extraction of 4',5,7-trihydroxyflavone from Artemisia annua and its application in the preparation of antibacterial drugs, relating to the technical field of medicine. The present invention uses petroleum ether, ethyl acetate, chloroform, and n-butanol to extract the medicinal material powder of Artemisia annua, and obtains extract powders of various polarities after concentration. Through antibacterial activity research, it is found that the polar parts of ethyl acetate and chloroform have significant activity . For the ethyl acetate and chloroform parts, the active fractions were obtained by silica gel column chromatography, thin-layer chromatography sampling method, and antibacterial activity experiment, and the high-purity was identified by high-performance liquid chromatography-mass spectrometry (LC-MS TOF) Fraction, and carry out MS, 1HNMR and 13CNMR analysis to this monomer fraction, obtain compound apigenin. The experimental method uses silica gel column chromatography to separate the polar parts. The separation method is simple and fast, and has certain practical significance.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the extraction of 4',5,7-trihydroxyflavone from Artemisia annua and its application in the preparation of antibacterial drugs. Background technique [0002] The aboveground dry part of Artemisia anomala S.Moore, a plant of the genus Artemisia in the family Compositae, is a perennial herb, and the whole plant shows flavonoid glycoside reaction. Produced in southern Jiangsu, it grows on hillsides, roadsides and forest edges. Distributed in East China, Central South and Southwest provinces. Also known as Nanliu Jinu, Jin Jinu, Liuyueshuang (Zhejiang, Jiangxi), Yema Lantou (Zhejiang), Jiuniucao (Hunan), Kusupo / Kingpocai (Fujian), Junexue / June Shuang (Anhui, Jiangxi, Hunan), Artemisia fragrans, and Capillaris (Jiangsu), are pungent, bitter, flat in nature and flavor. It was first recorded in "Lei Gong Concocting Theory" in the Southern and Northern Dynasties, and the main herbal m...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/30C07D311/40A61P31/04
Inventor 谭蔚锋吕磊张国庆张海曹青青陈俊
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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