Bacteria culture medium product containing feather meal as plant leftover

A technology of bacterial culture medium and feather powder, which is applied in the direction of bacteria, can solve the problems of high cost and efficient resource utilization of peptone, and achieve the effect of reducing production cost and efficient utilization

Active Publication Date: 2014-07-16
NANJING AGRICULTURAL UNIVERSITY +1
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  • Abstract
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Problems solved by technology

[0004] The purpose of the present invention is to develop a new type of bacterial culture medium ...

Method used

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  • Bacteria culture medium product containing feather meal as plant leftover
  • Bacteria culture medium product containing feather meal as plant leftover
  • Bacteria culture medium product containing feather meal as plant leftover

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Experimental program
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Embodiment Construction

[0021] 1 Analysis of amino acid content in feather meal

[0022] The waste feather meal purchased from Xinyi Hanling Biological Engineering Co., Ltd. was tested. The water content was 6.67%, the total nitrogen content was 17.91%, and the electrical conductivity was 49.32ms / cm. The free amino acids contained in the feather meal were tested. Extract and determine. The extraction method refers to GB / T18246-2000, and the automatic amino acid analyzer Biochrom30 is selected for determination. The results showed that feather meal contained a large amount of free amino acids, the total amount reached 44.31% (dry weight), and it was rich in variety, containing 14 common free amino acids.

[0023] Table 1 The content of free amino acids in feather meal

[0024]

[0025] 2 Feather meal instead of peptone culture model bacteria Bacillus subtilis and Escherichia coli

[0026] In order to verify whether the medium made of feather meal instead of peptone can ferment bacteria effectively, first sel...

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Abstract

The invention provides a bacteria culture medium product containing a feather meal as a plant leftover. The bacteria culture medium product is developed by replacing peptone in an LB (Lysogeny Broth) culture medium by the feather meal as the plant leftover and can be used for effectively culturing model escherichia coli, bacillus subtilis, other gram-negative bacteria and other gram-positive bacteria by virtue of liquid shake flask fermentation, wherein the feather meal used by the product is prepared by the steps of carrying out acidolysis on feathers, extracting plant leftovers of a part of amino acid, and carrying out neutralization and concentration to obtain the feather meal. Whether partly or wholly replacing the peptone in the LB culture medium, the bacteria culture medium product can both be used for effectively carrying out liquid fermentation on a strain for test. Meanwhile, By virtue of adopting a novel feather meal culture medium, the biomasses of the model escherichia coli and the bacillus subtilis in a stable period can be remarkably increased.

Description

Technical field [0001] The invention belongs to the field of biotechnology and provides a bacterial culture medium product developed from factory waste feather meal. Background technique [0002] Feather meal is a powdery substance made from feathers that are processed by physical or chemical methods. The morphological and chemical structure of feathers changes after treatment, and the double bond of keratin also changes accordingly to become a protein that can be digested and absorbed by animals. The development and research of feather meal has a history of more than 50 years abroad. Feather meal processing industries of various scales have been established all over the world, especially in industrialized countries. Japan produces 160,000 tons of feather protein per year, and the use of feather protein in the United States in 1969 reached 180,000 tons. my country has also begun to attach importance to feather protein resources, and a large number of companies extract soluble pr...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 沈其荣李荣曹亮亮马婧梁秋亮徐阳春
Owner NANJING AGRICULTURAL UNIVERSITY
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