2 results about "Enzymes levels" patented technology

The invention discloses an MIF small-molecule inhibitor as well as a screening method and application thereof, and belongs to the technical field of medicinal chemistry. The inhibitor is composed of seven compounds with specific structures and pharmaceutically acceptable salts thereof, and the compounds and the pharmaceutically acceptable salts can effectively inhibit the tautomerase activity of MIF. The lead compounds are efficiently found from a large number of compounds through a computer-aided virtual screening method. In-vitro and in-vivo experiments show that the compound F3277-0933, especially the compound F3277-0933, shows remarkable MIF inhibitory activity and anti-tumor effect in enzyme level, cellular level and animal models, and the effect of the compound F3277-0933 is superior to that of an existing inhibitor ISO-1. The MIF small-molecule inhibitor disclosed by the invention provides a new candidate compound for developing medicines for treating MIF-related diseases such as colorectal cancer.

The application discloses a kind of for waste PET depolymerization system glycol directional efficient conversion of glycolic acid two enzyme level connection system and its application, belong to biological catalysis and resource recycling technical field.The system includes a kind of mutant alcohol dehydrogenase with high selectivity to glycol and a mutant aldehyde dehydrogenase ALDH-V7.Said mutant alcohol dehydrogenase is obtained in at least one combination mutation in 97 and 101 and / or 145 and 148 in the basis of ADH5 enzyme derived from acetic acid bacillus ( Gluconobacter oxydans ).The system can efficiently and directionally oxidize low-value glycol in waste PET chemical or biological depolymerization product to high-value-added product glycolic acid under mild conditions, effectively avoiding peroxidation and C-C bond rupture.The application provides an efficient and green biological catalysis solution for upgrading and recycling of waste PET, and realizes a circular economy path of "waste plastics-degradable plastic monomers".