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33 results about "Enzymes levels" patented technology
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Trionyx sinensis breeding feed and its use and preparation method
InactiveCN102511672ADefensiveImprove absorption rateAnimal feeding stuffAquatic animalEnzymes levels
The invention discloses trionyx sinensis breeding feed and its use and preparation method. The trionyx sinensis breeding feed comprises trionyx sinensis compound feed and nano-zinc oxide mixed with the trionyx sinensis compound feed. Nano-zinc oxide is a nanoscale nutrient, can diffuse by passive transport so that the physical agility of an aquatic animal is saved, can be transported to tiny blood capillaries so that a zinc absorption rate is improved. Nano-zinc oxide can diffuse fast and thus a body can synthesize zinc-containing substances in a shortest period so that it is caused that the changes of a zinc-containing enzyme level, the secretion of a hormone related to zinc and zinc finger proteins influence the synthesis and the level of insulin, sex hormones and zinc finger proteins. Therefore, protein synthesis is promoted; nitrogen utilization efficiency of an animal is improved; excretion of nitrogen of manure and urine is reduced; and the pollution produced by nitrogen on the environment is reduced. Nano-zinc oxide has a certain moldy feed defense capability and thus when moldy feed enters into an intestinal tract, nano-zinc oxide can adsorb mycin of the moldy feed and discharge the mycin from the body so that disease prevention is realized and a physical ability of an aquatic animal is improved.
Owner:PUTIAN TIANXIAYUZHUANG ECOLOGY AGRI
Coelenterazine analog and preparing method and application thereof
ActiveCN105968114AHas the property of chemiluminescenceHigh luminous intensityOrganic chemistryChemiluminescene/bioluminescencePharmacologic actionEnzyme level
The invention discloses a coelenterazine analog and a preparing method and application thereof. The coelenterazine analog has the general structure formula (I) shown in the description, wherein R1, R2 and R3 in the formula are different substituents. According to application of the compound as a bioluminescence substrate, the existence and quantity (including the enzyme level, the cell level and the animal level) of coelenterazine luciferase can be detected with bioluminescence, and the in vitro, cell and in vivo distribution imaging of luciferase can be detected; the compound can serve as a report signal to detect the pharmacologic action and the toxic effect of medicine on the enzyme level, the cell level and the animal level under the existence of luciferase.
Owner:SHANDONG UNIV
Control of metabolic flux in cell-free biosynthetic systems
InactiveUS20160115558A1Increase leakage of electronMicrobiological testing/measurementEnzymesBiotechnologyTransport system
Owner:THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV +1
Amide derivative and purpose thereof
The invention relates to an amide derivative and a purpose thereof. The invention discloses an N-(4-substiguted aminoacyl phenyl)-5-substited furan-2-formamide compound and tests the activity of the compound for inhibiting falcipain-2 in experiment of enzyme level in vitro, and the result indicates that the compound has strong inhibitory activity to falcipain- 2. Therefore, the N-(4-substiguted aminoacyl phenyl)-5-substited furan-2-formamide compound provided in the invention can act as cysteine protease (falcipain-2) inhibitor in food vacuole of plasmodium to stop further proliferation of plasmodium in a host.
Owner:EAST CHINA UNIV OF SCI & TECH +1
Benzene alkyl amides compound and usage thereof
The invention relates to a substituted benzene alkyl amides compound, i.e. 4-(N-substituted amino-sulfonyl) benzene alkyl amide compound and a usage thereof. The inventor makes comprehensive use of CADD and SPR experiment based on Biacore, finds the substituted benzene alkyl amides compound and applies a reasonable drug design method to modify the structure of the substituted benzene alkyl amides compound so as to finally obtain the 4-(N-substituted amino-sulfonyl) benzene alkyl amide compound; after tests, the obtained 4-(N-substituted amino-sulfonyl) benzene alkyl amide compound inhibits the activity of falcipain-2 in in-vitro enzyme level experimental tests; and the result shows that the 4-(N-substituted amino-sulfonyl) benzene alkyl amide compound has strong falcipain-2 activity inhibiting capability and can be used for preparing medicaments for treating malaria caused by plasmodium.
Owner:EAST CHINA UNIV OF SCI & TECH +1
Naphthenic-base mono substituted amino fluorescein compound as well as preparation method and application thereof
ActiveCN104311504AQuick responseIncreased sensitivityOrganic chemistryFluorescence/phosphorescencePharmacologic actionEthyl group
The invention discloses a naphthenic-base mono substituted amino fluorescein compound as well as a preparation method and application thereof. The naphthenic-base mono substituted amino fluorescein compound has a structural general formula (described in the specification) or a free form thereof, wherein n is 0-9, and m is 1-9; when n is equal to m, R is mono substituted groups such as a hydrogen group, methyl, ethyl, propyl and halogen; and when n is not equal to m, R is the hydrogen group. The naphthenic-base mono substituted amino fluorescein compound can be used for detecting distribution and imaging of luciferase in vitro, cells and vivo by detecting the presence and quantity (including enzyme level, cellular level and animal level) of the luciferase by virtue of bioluminescence, can be also used for detecting distribution and imaging of ATP in vitro, cells and vivo by detecting the presence and quantity (including enzyme level, cellular level and animal level) of ATP and can be used for detecting pharmacological action of a medicine in enzyme level, cellular level and animal level as a report signal in the presence of luciferase, ATP and Mg<2+>.
Owner:SHANDONG UNIV
In-vitro model construction method for predicting toxicity of compound upon fatty liver diseases
InactiveCN109337863AHas adipocytic propertiesLower levelCompound screeningApoptosis detectionHepaticaEnzymes levels
The invention relates to an in-vitro model construction method for predicting toxicity of a compound upon fatty liver diseases. The method comprises the following steps: culturing different liver cells under a two-dimensional or three-dimensional condition, and culturing a fat converted liver cell in-vitro culture model by using a culture medium with a free fatty acid component. By adopting the in-vitro culture system, a growth micro environment in a liver cell can be simulated, the morphology and the function of the liver cell can be maintained for a long time, a cytochrome enzyme level is approximate to that in vivo, and the model has typical characteristics of fat converted liver cells, is capable of replacing fatty liver animal models, and achieving toxicology metabolism detection or pharmacodynamics study on a compound in long-term action on fat converted liver tissue in vitro.
Owner:NANJING DRUM TOWER HOSPITAL
Compound C6 as histone methyltransferase NSD3 activity inhibitor and application thereof
ActiveCN109223794AEnhanced inhibitory effectAntiproliferativeAntineoplastic agentsHeterocyclic compound active ingredientsChemical structurePurine
A C6 compound as histone methyltransferase NSD3 activity inhibitor and its pharmaceutical use are disclosed. C6 has a chemical structure represented by formula I. Chemical name is 6-Amino-9-(2-(naphthalen-1-Yloxy) ethyl)-9H-Purine-8-Thiol; At least one of that compound C6 or its hydrate, pharmaceutically acceptable salt, tautomers, stereoisomer. And precursor compounds is used as an active ingredient for preparing an antineoplastic drug. The pharmaceutical use of the compound C6 or its hydrate, the pharmaceutically acceptable salt, the tautomers, the stereoisomers. And the precursor compoundsare described. Experiments show that the compound C6 of the invention can effectively inhibit the NSD3 enzyme activity. And the IC50 value of the enzyme level is 17.97 +-2. 75[mu]mol / L. And significantly inhibited the proliferation of non-small cell lung cancer cell lines H460, H1299 and H1650. The compound of the invention has the effect of inhibiting tumor cell proliferation. And is expected tobe used as an active ingredient for preparing an anti-tumor drug. And has pharmaceutical prospect.
Owner:普美瑞(常州)生物科技有限公司
Chinese composition and preparation method and application thereof
InactiveCN101837049AClean up thoroughlyLiver function returns to normalDigestive systemPlant ingredientsToxic materialEnzymes levels
The invention relates to a Chinese composition and a preparation method thereof. The Chinese composition is prepared from the following components in part by weight: 100 to 400 parts of bupleurum, 50 to 300 parts of lucid ganoderma, 50 to 400 parts of root of red-rooted salvia and 100 to 450 parts of Chinese magnoliavine fruit. The Chinese composition has the effects of clearing away heat and toxic material, promoting urination and expelling toxin, protecting liver and decreasing enzyme level, promoting blood circulation and removing blood stasis, tonifying spleen and removing dampness through diuresis, recovering normal liver function, completely clearing viruses and the like.
Owner:XIAN BOAI PHARMA
Compound A8 used as histone transmethylase NSD3 activity inhibitor and application thereof
ActiveCN108853110AValidation of antitumor activityEnhanced inhibitory effectAntineoplastic agentsHeterocyclic compound active ingredientsChemical structurePurine
The invention discloses a compound A8 used as a histone transmethylase NSD3 activity inhibitor and a medicine purpose thereof. The compound A8 has a chemical structure shown as a formula I in description, and the chemical name is 6-amino-9-(2-(p-tolyloxy)ethyl)-9H-purine-8-thiol. The medicine purpose refers to the preparation of anti-tumor medicine by using at least one of the compound A8 or hydrates, pharmaceutically acceptable salt, dynamic isomers, stereoisomers and precursor compounds as active ingredients. Experiments show that the compound A8 can effectively inhibit the NSD3 enzyme activity; the enzyme level IC50 value is 0.69+ / -0.06mumol / L; the growth and proliferation of a non-small-cell lung cancer cell line H460 can be obviously inhibited. The compound has the effect of inhibiting the tumor cell proliferation, is hopeful to being used as an active ingredient for preparing the anti-tumor medicine and has medicine prospects.
Owner:普美瑞(常州)生物科技有限公司
C-6 site modified NanoLuc type analogues and preparation method and application thereof
ActiveCN111116594ACompensating for unsuitability for in vivo bioluminescent imagingLong glow timeOrganic chemistryChemiluminescene/bioluminescencePharmacologic actionEnzymes levels
The invention provides C-6 site modified NanoLuc type analogues and a preparation method and application thereof. The C-6 site modified NanoLuc type analogues have a structure as shown in a formula Iwhich is described in the specification. In the formula I, R1 is selected from a group consisting of a 4-fluorophenyl group, a 4-chlorphenyl group, a 4-bromophenyl group, a 3-fluoro-4-aminophenyl group, a thienyl group, a furyl group, a 5-methylfuryl group, a naphth-2-yl group, a benzofuryl group, a 4-methylphenyl group, a 3,5-dimethylphenyl group, a 4-hydroxymethylphenyl group, a 4-methoxyphenylgroup and a 4-mercaptophenyl group. The analogues can be used as a bioluminescent substrate for researching protein-protein and protein-ligand interactions, monitoring protein stability and bioluminescence resonance energy transfer and the like. The invention provides application of the analogues to in-vitro, cellular and in-vivo distribution imaging under the action of NanoLuc luciferase in virtue of bioluminescence and application of the analogues as a report signal to detect the pharmacological action and toxic action of a drug at an enzyme level, a cellular level and an animal level in thepresence of NanoLuc luciferase.
Owner:SHANDONG UNIV
Medicine for inhibiting Marek's disease virus replication and preventing or resisting Marek's disease
ActiveCN112972476AInhibitory activityInhibit tumor growthOrganic active ingredientsAntiviralsDeubiquitinating enzymeMarek's disease
The invention provides a medicine for inhibiting Marek's disease virus replication, which comprises a deubiquitinating enzyme UL36 inhibitor and a medicine for preventing or resisting Marek's disease. The deubiquitinating enzyme UL36 inhibitor DUBs-IN-1 and the derivative thereof are screened out for the first time through an enzyme activity experiment for inhibiting virus encoding, a drug toxicity experiment on a CEF cell level, a virus CPE inhibition experiment and a chicken Marek's disease model experiment, the activity of UL36 can be inhibited on an enzyme level, replication of Marek's disease viruses can be inhibited on a cell level, and the Marek's disease tumor growth and visceral hemorrhage can be inhibited on the animal level, has the advantages of extremely strong effect and small dosage, and can be widely applied to production practice of Marek's disease prevention in poultry industry as an anti-MD drug.
Owner:JILIN UNIV
Mateirals and methods for alteration of enzyme and acetyl coA levels in plants
InactiveCN1322252AIncreased or decreased enzyme levelsLevel up or downHydrolasesTransferasesAcetyl Coenzyme A SynthetasePlant cell
The invention provides acetyl-CoA synthase (ACS), plastid pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL), Arabidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehydes Dehydrogenase (ALDH), specifically the nucleic acid sequence and amino acid sequence of ALDH-2 and ALDH-4. The invention also provides a recombinant vector, which contains a nucleic acid sequence encoding one of the above-mentioned enzymes, its antisense sequence or its ribozyme. In cells transformed by such a vector, the antibodies of these enzymes are changed at the enzyme level. A plant cell, a plant tissue, a plant organ or a plant, and methods of producing such plant cells, plant tissue, plant organs or plants. Ideally, changes in enzyme levels result in changes in acetyl-CoA levels in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector, including encoding pyruvate decarboxylase (PDC), the E1α subunit of pPDH, the E1β subunit of pPDH, the E2 subunit of pPDH, mitochondrial pyruvate dehydrogenase (mtPDH) or aldehyde Antisense sequence of the nucleic acid sequence of dehydrogenase (ALDH), or a ribozyme that can cleave RNA molecules encoding PDC, E1αpPDH, E1βpPDH, E2pPDH, mtPDH or ALDH.
Owner:IOWA STATE UNIV RES FOUND
Application of flavonoid products in preparation of drug for treating cancer and inhibitor
InactiveCN108743579AImprove biological activityLarge research spaceOrganic active ingredientsInorganic active ingredientsEnzyme levelEnzymes levels
The invention relates to an application of flavonoid products in preparation of a drug for treating cancer and an inhibitor. The flavonoid products are myricetin, a dihydroxylation form of myricetin or a dihydroxylation form of quercetin, and an Mg<2+> preparation is used during application. The bioactive range of natural flavonoid on the enzyme level is extended, and a road is paved for developing flavonoid assisted targeted cancer treatment. Besides, great research space exists in researching the function of myricetin on 5'-ribozyme family and combination of myricetin with other drugs to target DDR (DNA damage response) for cancer treatment.
Owner:TIANJIN UNIV OF SCI & TECH
A kind of construction method and application of recombinant bacteria producing chitosanase
The invention discloses a construction method and application of a recombinant bacterium producing chitosanase. The invention co-expresses the Bacillus subtilis chitosanase gene and the hyaline hemoglobin gene in Pichia pastoris, and the recombinant strain efficiently prepares the chitosanase through a high-density fermentation strategy. The enzyme activity of the chitosanase prepared by the method is as high as 50000 U / mL, and the protein content is as high as 16.0 mg / mL. The specific enzyme activity of the recombinant enzyme is 4065.7U / mg, the optimum pH is 6.0, and the optimum temperature is 55°C. The enzyme hydrolyzes high-concentration (≥10%) chitosan to prepare chitosan oligosaccharides, the chitosan oligosaccharide yield is greater than 75%, and the main products are disaccharides, trisaccharides and tetrasaccharides. The chitosanase preparation method of the present invention produces enzyme level far higher than the reports of existing documents and patents, and the produced enzyme has excellent hydrolysis characteristics, and has good performance in industrial production of chitosanase and preparation of chitosan oligosaccharides. application value.
Owner:CHINA AGRI UNIV
Coelenterazine-h-based deuterated compound as well as preparation method and application thereof
ActiveCN113683617AShow concentration dependenceExcellent bioluminescence intensityIsotope introduction to heterocyclic compoundsMicrobiological testing/measurementChemical compoundCombinatorial chemistry
The invention discloses a coelenterazine-h-based deuterated compound and a preparation method and application thereof, the chemical structural formula is as follows, wherein R1 is phenyl or deuterated phenyl, R2 is hydrogen or deuterium, R3 is hydrogen or deuterium, R4 is hydrogen or deuterium, R5 is hydrogen or deuterium, R6 is hydrogen or deuterium, and at least one of R1-R6 is a deuterated group. According to the application of the deuterated compound as a bioluminescent substrate, the in-vivo and in-vitro distribution condition of luciferase can be monitored. The bioluminescence intensity of the deuterated substance of coelenterazine-h is superior to that ofthe coelenterazine-h at the enzyme level, the cell level and the animal level . Meanwhile, the preparation method of the deuterated compound of coelenterazine-h, provided by the invention, is simple, classical in reaction steps, high in operability and low in cost, and has a good practical value.
Owner:SHANDONG UNIV
Amide derivative and purpose thereof
The invention relates to an amide derivative and a purpose thereof. The invention discloses an N-(4-substiguted aminoacyl phenyl)-5-substited furan-2-formamide compound and tests the activity of the compound for inhibiting falcipain-2 in experiment of enzyme level in vitro, and the result indicates that the compound has strong inhibitory activity to falcipain- 2. Therefore, the N-(4-substiguted aminoacyl phenyl)-5-substited furan-2-formamide compound provided in the invention can act as cysteine protease (falcipain-2) inhibitor in food vacuole of plasmodium to stop further proliferation of plasmodium in a host.
Owner:EAST CHINA UNIV OF SCI & TECH +1
Trionyx sinensis breeding feed and its preparation method
The invention discloses trionyx sinensis breeding feed and its use and preparation method. The trionyx sinensis breeding feed comprises trionyx sinensis compound feed and nano-zinc oxide mixed with the trionyx sinensis compound feed. Nano-zinc oxide is a nanoscale nutrient, can diffuse by passive transport so that the physical agility of an aquatic animal is saved, can be transported to tiny blood capillaries so that a zinc absorption rate is improved. Nano-zinc oxide can diffuse fast and thus a body can synthesize zinc-containing substances in a shortest period so that it is caused that the changes of a zinc-containing enzyme level, the secretion of a hormone related to zinc and zinc finger proteins influence the synthesis and the level of insulin, sex hormones and zinc finger proteins. Therefore, protein synthesis is promoted; nitrogen utilization efficiency of an animal is improved; excretion of nitrogen of manure and urine is reduced; and the pollution produced by nitrogen on the environment is reduced. Nano-zinc oxide has a certain moldy feed defense capability and thus when moldy feed enters into an intestinal tract, nano-zinc oxide can adsorb mycin of the moldy feed and discharge the mycin from the body so that disease prevention is realized and a physical ability of an aquatic animal is improved.
Owner:PUTIAN TIANXIAYUZHUANG ECOLOGY AGRI
In-vitro composition method for evaluating hair growing/hair loss prevention effect
InactiveCN108531543APromote proliferationPromote circulationCompound screeningApoptosis detectionCytotoxicityEnzymes levels
The invention discloses an in-vitro composition method for evaluating a hair growing / hair loss prevention effect. The in-vitro composition method comprises the following steps: S1, analyzing a 5alphareductase inhibition level; S2, carrying out cell effect analysis: analyzing cytotoxicity, a cell proliferation capability and an anti-oxidization capability; S3, analyzing angiogenesis; S4, evaluating a result and predicating. The in-vitro composition method disclosed by the invention is used for screening functionalized cosmetic raw materials through various biology methods and through a combined enzyme level test, a cell test and a tissue level test; a testing system is simple and low in price; special equipment is not needed; a detection is rapid, flexible and universal. The in-vitro composition method can be used for qualitatively and evaluating effects of hair growing raw materials; the method disclosed by the invention also can be used for replacing live animals and human volunteersand is used for screening and detecting the toxicity and effects of the hair growing and hair loss prevention raw materials.
Owner:黄健聪 +1
Cydonia oblonga-ocimum basilicum total flavonoid composition and application thereof in atherosclerosis
ActiveCN110327392AGood curative effectReduce formationCardiovascular disorderPlant ingredientsBlood vessel injuryEnzymes levels
The invention discloses a cydonia oblonga-ocimum basilicum total flavonoid composition and application thereof in atherosclerosis. The cydonia oblonga-ocimum basilicum total flavonoid composition comprises the following components in parts by weight: 1-3 parts of cydonia oblonga total flavonoid and 1-3 parts of ocimum basilicum total flavonoid. A test of the cydonia oblonga-ocimum basilicum totalflavonoid composition for atherosclerotic rats shows that: the cydonia oblonga-ocimum basilicum total flavonoid composition can effectively reduce blood fat and blood vessel injury factor level, increase antioxidant enzyme level, improve organism oxidation resistance, reduce oxide formation, increase blood vessel protection factor level, effectively protect blood vessel, improve liver function andprevent lipid metabolism disorder. The synergistic effect of the cydonia oblonga-ocimum basilicum total flavonoid composition improves the curative effect on atherosclerosis, and further provides a theoretical basis for preventing and treating atherosclerosis.
Owner:XINJIANG MEDICAL UNIV
A Class of Dual Responsive Fluorescent Dye Molecules
ActiveCN110835303BHigh sensitivityHigh selectivityAmino preparation from aminesGroup 5/15 element organic compoundsNitroreductaseNiacinamide
A class of dual-response fluorescent dye molecules has a structure of general formula I. The molecule has a dual-line response characteristic to nitroreductase (NTR) and nicotinamide adenine dinucleotide (NADH) in vivo. Specific detection can be performed for both enzyme levels. At the same time, this kind of probe has good water solubility, good photostability, low phototoxicity, photobleaching and excellent biological toxicity. It has a good application prospect in biological detection.
Owner:HENAN NORMAL UNIV
Compound C1 used as histone methyltransferase NSD3 activity inhibitor and application thereof
ActiveCN109223793AEnhanced inhibitory effectAntineoplastic agentsHeterocyclic compound active ingredientsTumor targetChemical structure
Owner:普美瑞(常州)生物科技有限公司
Pediatric myocarditis treating formula
InactiveCN105267298AGood heart muscleNourish the myocardiumAntiviralsCardiovascular disorderPediatric patientViral Myocarditis
The present invention provides a pediatric myocarditis treating formula, wherein the components comprise, by weight, 10 g of honeysuckle, 10 g of raw milkvetch root, 6 g of cyrtomium fortunei, 6 g of atractylodes macrocephala, 10 g of saposhnikovia divaricata, 6 g of atractylodes lancea, 10 g of agastache rugosa, and 10 g of adenophora stricta miq. According to the present invention, the pediatric myocarditis treating formula is suitable for treatment of a variety of pediatric myocarditis caused by viruses, the effect is good, the cardiac muscle of the pediatric myocarditis patients can be nourished and the cardiac function can be improved through the formula, the pediatric myocarditis pathological change development can be effectively prevented, the myocardial enzyme level of the pediatric patients can be significantly improved, and various indexes of the body can be effectively promoted to achieve the normal levels.
Owner:高晓燕
A kind of coelenterazine analogue and its preparation method and application
ActiveCN105968114BHas the property of chemiluminescenceHigh luminous intensityOrganic chemistryChemiluminescene/bioluminescencePharmacologic actionEnzyme level
The invention discloses a coelenterazine analog and a preparing method and application thereof. The coelenterazine analog has the general structure formula (I) shown in the description, wherein R1, R2 and R3 in the formula are different substituents. According to application of the compound as a bioluminescence substrate, the existence and quantity (including the enzyme level, the cell level and the animal level) of coelenterazine luciferase can be detected with bioluminescence, and the in vitro, cell and in vivo distribution imaging of luciferase can be detected; the compound can serve as a report signal to detect the pharmacologic action and the toxic effect of medicine on the enzyme level, the cell level and the animal level under the existence of luciferase.
Owner:SHANDONG UNIV
A cycloalkyl monosubstituted aminofluorescein compound and its preparation method and application
ActiveCN104311504BQuick responseIncreased sensitivityOrganic chemistryFluorescence/phosphorescenceHalogenHydrogen
Owner:SHANDONG UNIV
Amino acid-fluorophore compound and application thereof
The invention relates to an amino acid-fluorophore compound and application of the compound, wherein the general structural formula of the amino acid-fluorophore compound is (I), (II), (III), and (IV) or a free form thereof, wherein R1 is selected from various amino acid side chains, preferably methyl, propyl, 2-methylthio ethyl, benzyl, phenylethyl, 2-cyclohexyl ethyl, 4-isopropyl phenyl and 4-dimethyl amino phenyl; R2 is selected from various fluorophores, preferably 7-hydroxyl coumarin, naphthalimide fluorophores, Nile red series and Cy fluorophores; and X is C or N. Fluorescent probe molecules can be used for detecting the activity (enzyme level and cell level) of aminopeptidase N, can be used as a probe tool for detecting the tissue distribution of aminopeptidase N and tumor tissue imaging, and can be used as a diagnosis tool for various diseases due to abnormal expression of aminopeptidase N. In addition, the preparation method of the compound has the advantages of mild reaction conditions, easily-accessible and cheap raw materials and simplicity in operation and after-treatment.
Owner:SHANDONG UNIV
A kind of thermophilic fungus cutinase and its coding gene and application
ActiveCN108424894BGreat potentialCan catalyze esterification reactionFungiHydrolasesEnzymes levelsDe esterification
The invention discloses a thermophilic fungus cutinase, its coding gene and application. The cutinase is a protein of the following 1) or 2) or 3): 1) a protein consisting of the amino acid sequence shown in sequence 2 in the sequence listing; 3) The protein shown in 1) or 2) undergoes substitution and / or deletion and / or addition of one or several amino acid residues and has cutinase activity. The recombinant bacteria formed by introducing the protein coding gene into Pichia pastoris were fermented at high density in a 5L fermenter, and the enzyme activity of the fermentation broth could reach 12536U / mL, and the protein content was 10.8mg / mL. The cutinase can hydrolyze butter to prepare milky flavor; catalyze the esterification reaction of short carbon chain alcohol and acid to synthesize flavor ester. The cutinase has good thermal stability and high enzyme production level, and has the potential of industrial production and application.
Owner:CHINA AGRI UNIV
Compound B6 as histone methyltransferase NSD3 activity inhibitor and application thereof
ActiveCN109223796AValidation of antitumor activityEnhanced inhibitory effectAntineoplastic agentsHeterocyclic compound active ingredientsChemical structurePurine
A B6 compound as histone methyltransferase NSD3 activity inhibitor and its pharmaceutical use are disclosed. B6 has a chemical structure represented by formula I. Chemical name: 6-Amino-9-(2-(4-Isopropoxyphenoxy) ethyl)-9H-Purine-8-Thiol; At least one of that compound B6 or its hydrate, pharmaceutically acceptable salt, tautomer, stereoisomer. And precursor compound is used as an active ingredientfor preparing an antineoplastic drug. Experiments show that the compound B6 of the invention can effectively inhibit the NSD3 enzyme activity. And the IC50 value of the enzyme level is 0.82 +- 0.17 [mu]mol / L, and significantly inhibited the growth and proliferation of non-small cell lung cancer cell line H460. The compound of the invention has the effect of inhibiting tumor cell proliferation, and is expected to be used as an active ingredient for preparing an anti-tumor drug. And has pharmaceutical prospect.
Owner:普美瑞(常州)生物科技有限公司
Chitosanase ouc-csnca and its application
ActiveCN111500555BIncrease enzyme activityHigh enzyme productionBacteriaMicroorganism based processesEnzymes levelsOligosaccharide
The invention discloses a chitosanase OUC-CsnCA, the amino acid sequence of which is shown in SEQ ID NO.1. The present invention also discloses the gene encoding the chitosanase OUC-CsnCA, the nucleotide sequence of which is shown in SEQ ID NO.2. The application of the chitosanase OUC-CsnCA in degrading chitosan / preparing chitosan oligosaccharides, and in the preparation of enzyme preparations for degrading chitosan. Chitosanase OUC-CsnCA of the present invention is used to degrade chitosan to produce chitosan oligosaccharide, its optimum pH is 8.0, optimum reaction temperature is 55 ℃, and specific enzyme activity is 1786.227U / mg; Enzyme activity and production The enzyme level is high, and the protein concentration after purification is 1.284mg / ml, which has certain advantages compared with the reported chitosanase. The chitosanase OUC-CsnCA of the invention has important industrial application value and economic value.
Owner:OCEAN UNIV OF CHINA
Application of haloferula sp.β-N-acetylhexosaminidase in the synthesis of human milk oligosaccharides
ActiveCN111534503BExcellent transglycosidic activityHigh enzyme productionFermentationVector-based foreign material introductionGlycosideOligosaccharide synthesis
The invention discloses the application of Haloferula sp.β-N-acetylhexosaminidase in synthesizing human milk oligosaccharides. The present invention provides the application of HaHex74 protein or its related biological materials in any of the following: (a1) synthesis of human milk oligosaccharides; (a2) synthesis of lactose-N-triose II and / or lactose-N-neotetrasaccharide; The HaHex74 protein is derived from Haloferula sp., and its amino acid sequence is shown in SEQ ID No.1. The β-N-acetylhexosaminidase of the present invention has high enzyme production level, excellent hydrolysis characteristics and high transglycoside transfection activity, and has important application value in enzymatically synthesizing human milk oligosaccharides.
Owner:CHINA AGRI UNIV
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