The invention provides a specific primer for trionychidae sex identification and a detection method thereof. The specific primer comprises a
forward primer 5 'GGTGATGGTGGTACTGGTAAAAC3'and a
reverse primer 5' GCAGACCACCAAATTTCCTG3 ', and the
forward primer 5' GGTGATGGGTGGTACTGTAAAAC3 'and the
reverse primer 5' GCAGACCACCAAATTTCCTG3 'are The method for carrying out sex identification by using the primer comprises the following steps: extracting
genome DNA of a trionychidae individual to be detected; carrying out PCR (
Polymerase Chain Reaction) amplification by adopting the specific primer; and carrying out
electrophoresis detection on the amplified product, judging that the amplified product is male when only 250bp bands appear, and judging that the amplified product is female when 250bp and 600bp bands appear at the same time. The method is suitable for sex determination and PCR amplification
system and program parameter optimization of trionychidae animals such as Chinese softshell turtles, horns and apalone ferox, and 1.5%
agarose gel electrophoresis detection is adopted. The invention also provides a trionychidae animal sex identification kit containing the primer pair, and the invention provides a rapid, accurate, simple and convenient trionychidae animal sex identification method, which can be applied to the breeding and cultivation management of trionychidae animals, and has high commercial value.