9 results about "Marek's disease" patented technology

The invention relates to the fields of molecular biology and virology, and provides a method for preparing a specific MHC haplotype-derived cell-binding virus. The method comprises the following steps: firstly, obtaining chick embryos of different haplotypes, separating CEF cells, transiently expressing exogenous fluorescent protein through electrotransfection, then carrying out virus inoculation, carrying out flow sorting to obtain a fluorescent positive cell population carrying MDV, and carrying out quantitative analysis on the cell population to obtain the specific MHC haplotype chicken Marek's Disease Virus (MDV), thereby obtaining the specific MHC haplotype chicken Marek's Disease Virus Marek's Disease Virus Marek's Disease Virus Marek's Disease Virus. And carrying out in-vitro propagation to obtain the purified MDV with a specific haplotype source. The purified virus can provide a test material for related research of haplotype on virus infection, and can be widely applied to in-vitro purification of different cell binding viruses and related research of specific MHC molecule presenting virus peptides. The method disclosed by the invention is beneficial to carrying out related research aiming at the host immune response of the virus and analyzing the hereditary basis of haplotype to MDV differential resistance, and is expected to be applied to preparation of vaccine virus.

The invention provides a molecular diagnosis method for avian leukosis virus and Marek's disease virus mixed infection. The molecular diagnosis method comprises the following steps: (S01) collecting diseased material tissues; (S02) carrying out nucleic acid extraction on the sick material tissue of the sick chicken; (S03) detecting the nucleic acid by using specific primers of avian leukosis and Marek's disease; (S04) preparing a PCR (Polymerase Chain Reaction) detection system, and respectively adding ALV-env gene amplification primers and MDV-meq gene amplification primers for gene amplification; (S05) carrying out 1% agarose gel electrophoresis detection on the amplification product in the step (4), and carrying out sequence determination after determining a target band; (S06) carrying out BLAST comparison on the sequences obtained by sequencing, carrying out gene sequence analysis and drawing an evolutionary tree; and (S07) determining the virus type according to the homology and the evolutionary branches, so as to realize rapid diagnosis of ALV and MDV mixed infection, strain characteristic analysis and exclusion of other similar epidemic diseases, and provide technical support for accurate prevention and control of AL and MD in the poultry industry, provenance purification and epidemiological investigation.

The present application belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition, a preparation method and application thereof. Specifically, based on the theory of traditional Chinese veterinary medicine of'strengthening the body resistance and eliminating pathogenic factors, and treating the root and branch', aiming at the pathogenesis of 'deficiency in the root and excess in the branch' of low immunity in animals, eight kinds of medicinal materials are scientifically screened to form a traditional Chinese medicine compound with the effects of tonifying qi and nourishing blood, invigorating the spleen and kidney, dredging collaterals and eliminating dampness, and clearing heat and detoxifying. Through the verification of a cyclophosphamide-induced low immunity mouse model, the traditional Chinese medicine composition can significantly improve the mental state of the mice, restore the body weight and thymus / spleen index, repair the pathological damage of the spleen tissue, and adjust the inflammatory factor level in the serum and spleen tissue. In addition, in a Marek's disease virus infection model, the traditional Chinese medicine composition combined with the Marek's disease virus vaccine can significantly reduce the viral load in the peripheral blood, liver and spleen, and reduce the liver enlargement, hemorrhage and microscopic structure damage, showing a good immune synergistic effect.

The application provides an amplification primer and method for a whole genome of chicken Marek's disease virus. The detection primer set of the chicken Marek's disease virus comprises 206 pairs of amplification primers, and specific sequences are shown as SEQ ID NO. 1-412. The detection primer set provided by the application can realize relatively uniform coverage of the chicken Marek's disease virus, and in nanopore sequencing, 100% area coverage of the genome is realized. The sequencing fragment preparation method for the whole genome of the chicken Marek's disease virus provided by the application is simple and easy to operate, has good amplification effect, and nanopore sequencing has the advantage of real-time analysis during sequencing, so that the detection time can be greatly shortened, and the infection of the chicken Marek's disease virus can be differentially diagnosed in 15h at most, meanwhile, the whole genome sequence thereof is obtained, and the obtained genome sequence can provide a scientific basis for virus tracing, pathogen variation tracking, new strain identification and early warning and the like.

The invention relates to the technical field of gene engineering, and discloses application of an inhibitor of LRP1 protein or a coding gene thereof in preparation of a preparation for resisting MDV infection. Specifically, the invention discloses application of the LRP1 protein or the coding gene thereof in regulation and control of Marek's disease virus (MDV) in-vitro infection of chicken embryo fibroblasts (CEF). The invention finds that the MDV infection rate can be remarkably reduced by knocking down the LRP1 gene in CEF cells, and further provides application of the LRP1 protein or the coding gene thereof or a biological material containing the coding gene thereof in resisting MDV infection. According to the invention, the effect of the LRP1 gene in the in-vitro infection of the CEF by the MDV is defined, and a foundation is laid for further research on the antiviral mechanism of the LRP1 gene and application of the LRP1 gene in disease-resistant breeding.

The present invention relates to recombinant Marek's disease viruses containing, inserted into at least one insertion site, at least one recombinant nucleotide sequence encoding an antigen operably linked to a promoter and an insulator, their manufacture, compositions comprising the same, and the uses thereof.

The application discloses a PCR primer and probe for identifying serum type I Marek's vaccine virus and wild virus, wherein the primer comprises an upstream primer with a sequence as shown in SEQ ID No:1 and a downstream primer with a sequence as shown in SEQ ID No:2; and the probe comprises a wild virus-specific probe with a sequence as shown in SEQ ID No:3 and a vaccine virus-specific probe with a sequence as shown in SEQ ID No:4. Through the primer and the probe, the serum type I Marek's vaccine virus and the wild virus can be efficiently, conveniently and quickly identified, so that early warning, accurate prevention and control and purification of Marek's disease are realized.

The present invention relates to the field of animal health. Particularly, the present invention relates to a recombinant Marek's Disease Virus (MDV) comprising at least one exogenous nucleotide sequence in the genome thereof, wherein the at least one exogenous nucleotide sequence is located at a position between UL55 and Lorf10, or a position between UL2 and UL3. Further, the present invention provides an immunogenic composition comprising the recombinant MDV of the present invention and the use of the immunogenic composition for preventing and / or treating diseases in an animal.

The application provides a characteristic protein of a serum type I chicken Marek's disease virus and a detection application, and belongs to the technical field of virus detection. The application provides a recombinant gene derived from the serum type I chicken Marek's disease virus, and the nucleotide sequence of the recombinant gene is shown as SEQ ID No. 1. The recombinant gene encodes MDV-1 recombinant PP38 protein, and the MDV-1 recombinant PP38 protein can be used for ELISA detection. The application also provides an ELISA detection kit constructed by using the MDV-1 recombinant PP38 protein, which can be used for clinical MDV-1 serum antibody detection, screening of SPF chickens, detection of exogenous virus antibodies of avian live vaccines or virus seeds and epidemiological investigation, so as to prevent and control the spread of MDV-1 infection.