57 results about "Spleen Tissue" patented technology

The present invention provides an antioxidative composition with high safety, which is capable of lessening oxidative stress due to active oxygen species, free radicals, or the like in vivo, thereby preventing the occurrence or worsening of a disease. It was confirmed that by using a composition containing oxidized coenzyme Q and / or reduced coenzyme Q, the amount of urinary 8-hydroxydeoxyquanosine can be decreased in normal or diabetic animals. Also, in histopathological research of the spleens of diabetic rats, it was confirmed that spleen tissue denaturation caused by oxidative stress can be prevented. It was thus found that oxidative stress in vivo can be lessened. According to the present invention, oxidative stress in vivo can be lessened by using a composition containing coenzyme Q as an active ingredient.

The invention discloses a spleen polypeptide extract extracted from the spleen tissue of mammals other than humans. The mammalian tissue is subjected to fat removal, homogenization, acid adjustment, freezing and thawing, pH adjustment, precipitation, heating, and centrifugation. Filtration, ultrafiltration and other steps to obtain the spleen polypeptide extract; the drug is an immunomodulatory drug that can improve and improve the immune function of the body, and can be used for primary and secondary cellular immune deficiency diseases (such as eczema, thrombocytopenia, multiple Infection syndrome, etc.), respiratory tract and lung infection, habitual cold, chronic hepatitis B, mumps, recurrent aphtha and other diseases, can be used in the treatment of leukopenia, leukemia, aplastic anemia caused by radiotherapy and chemotherapy , Lymphoma and other malignant tumors, to improve the malignant transformation of tumor patients, to improve postoperative or critically ill patients when they are weak.

The invention discloses a nanobody of the anti-GPC 3 (Glypican 3) protein and a preparation method and application of the nanobody. A prokaryotic expression GPC 3 fusion protein is used to immunize acamel, the RNA in a spleen tissue of the camel is extracted and is used as a template to implement inverse transcription to form cDNA, the cDNA is constructed to a carrier of an outer capsid protein of a bacteriophage to construct a bacteriophage display library, and the storage capacity and the titer are calculated. Then the nanobody specifically combined with the GPC 3 is screened from the library according to the antigen-antibody affinity principle after elutriating for three times, a gene of the specific antibody is then obtained, an expression carrier is constructed, the prokaryotic expression is implemented, the expression carrier is purified and identified, and the required nanobody is obtained.

The invention discloses a fullerene micro-nano material with effects of prevention and / or treatment on myelosuppression and a use thereof. The novel use of the fullerene micro-nano material comprises use of the fullerene and / or metallofullerene micro-nano material in a drug or a drug carrier with at least one of properties such as 1, prevention and / or treatment on myelosuppression, 2, prevention and / or treatment on at least one of myelosuppression-caused leucopenia, platelet decreasing, hemoglobin decreasing and monocyte decreasing, and 3, protection of liver tissue, spleen tissue and kidney tissue. The myelosuppression is caused through tumor radiation therapy or rays producing myelosuppression side effects. The fullerene and / or metallofullerene micro-nano material can prevent and treat myelosuppression and prevent liver, spleen and kidney damage.

The invention relates to a nano magnetic resonance imaging contrast agent and a preparation method thereof. The contrast agent consists of external aqueous phase and humic acid substance-modified super-paramagnetic ferrite nano crystal dispersed in the external aqueous phase. The super-paramagnetic ferrite nano crystal is synthesized by adopting a chemical co-precipitation method, and the grain diameter is 15 to 30 nanometers. The nano crystal is purified and then dispersed in the external aqueous phase to form the contrast agent for intravenous injection. The contrast agent can be stabilized for over two years. The contrast agent has low toxicity, good biocompatibility, large time window and high saturation magnetization, and the half-life period of the contrast agent in the blood reaches 30 to 60 minutes. The contrast agent is absorbed by netlike endothelial systems in liver and spleen tissues through intravenous injection, and is focused in the liver and spleen tissues so as to shorten the T2 relaxation time. In a T2 image, signals of normal liver and spleen tissues are obviously reduced, and signals of tumor or pathological change tissues have no obvious change, so the contrast agent improves the imaging contrast of the focus and the normal tissue and can be used for early diagnosis of pathological changes such as micro tumor of liver and spleen tissues and the like.

The present invention discloses a preparation method of a soft tissue decellular matrix, and relates to the technical field of clinical medicine, biomedical medicine and regenerative medicine. According to the present invention, the natural soft tissue decellular matrix is prepared by using a supercritical fluid technology, wherein the natural soft tissue comprises small intestine tissue, blood vessel tissue, muscle tissue, heart tissue, valve tissue, lung tissue, spleen tissue, kidney tissue, liver tissue, stomach tissue, gallbladder tissue, fat tissue, cartilage tissue, trachea tissue, esophagus tissue, bladder tissue, ureter tissue, oviduct tissue, or uterus tissue. According to the present invention, with the preparation method, the damage on the soft tissue matrix component is low, and the obtained soft tissue decellular matrix has advantages of low immunogenicity and good biocompatibility.

The invention discloses a micro-nano material for preventing and / or treating myelosuppression and application thereof. A fullerene and / or metal fullerene micro-nano material can be applied to preparing medicine with at least one of the following properties of preventing and / or treating myelosuppression, removing free radicals, preventing and / or treating at least one of leukocyte reduction, platelet reduction, hemoglobin reduction and monocyte reduction caused by myelosuppression, preventing bone marrow cells and / or hematopoietic cells, improving the function of antioxidant systems of organisms and protecting liver tissue, spleen tissue and kidney tissue. The fullerene and / or metal fullerene micro-nano material can be metabolized out of organisms, is enriched in visceral organs and marrow through blood circulation after intragastric administration or intraperitoneal injection, has an excellent function of preventing myelosuppression induced by chemotherapy in bodies, can effectively reduce toxic and side effects of chemotherapy on marrow and other organs, and is still obvious in protection effect after multiple treatment courses.

The invention discloses a flow cytometry detection method for mouse thymus or spleen T-lymphocyte subsets. The method comprises the following steps: acquiring thymus or spleen tissues of tested mice, and preparing single-cell suspension; respectively sucking the single-cell suspension of the thymus or spleen, respectively adding anti-mouse DC3, CD4 and CD8 monoclonal antibodies, swirling and uniformly mixing, keeping away from light at the temperature of 4 DEG C and staining; washing with PBS (Phosphate Buffer Solution) after staining, resuspending the cells, and detecting by using flow cytometry; and analyzing the detection result, thereby obtaining the percentage of each T-lymphocyte subset of the thymus or spleen of the mice. The stable and standardized method for detecting the mouse thymus or spleen T-lymphocyte subsets through flow cytometry is successfully established in the invention. By improving the problems such as target cell selection, compensative regulation and parameter selection in the sample pretreatment, detection and analysis process, errors caused by human factors in the test process are avoided, and the authenticity and objectivity of result judgment are improved.

The invention discloses a method for screening probiotics. The method comprises the following steps of: (1) carrying out a primary culture on spleen tissues of a pig or a domestic animal; (2) attacking toxicity of the tissues through colon bacillus K88, and testing variations of levels of an interleukin 8 and an interleukin 4; (3) respectively processing the tissues through candidate probiotic strains, and testing variations of levels of two interleukins; (4) comparing the variations to select probiotic strains which are similar in variation degrees of the two interleukins but opposite in effect due to treatment of the colon bacillus K88; and (5) through the colon bacillus K88 animal toxicity attacking test, observing protecting effects of primarily selected probiotic strains, and finally determining an efficient probiotic strain. The method for screening the probiotic disclosed by the invention has the remarkable advantages that: the method combining in vitro immunology experiment and in vivo toxicity attacking experiment is high in accuracy, strong in pertinency and time-saving; and the screened probiotics can effectively replace antibiotic, and greatly reduce diseases caused by colon bacillus so as to promote the health of the animal.

The invention discloses a peptide-containing milk and process for preparation. The milk of the invention is processed through charging spleen polypeptide extract extracted from fetal bovine spleen tissue into common milk, and then processing through homogeneous process. During preparing the spleen polypeptide extract, steps of homogenate, freezing and thawing, interception, filtering and the like are employed, thereby processing spleen polypeptide extract with molecular weight < 30000 Daltons. A lot of experiments prove that the peptide-containing milk of the invention has the function of enhancing body immunity.

The invention provides an identification method of a chicken salmonella enteritidis inflection methylation regulatory gene, and relates to the technical field of bio-genetic engineering, capable of identifying a gene taking an effect of methylation regulation in chicken salmonella enteritidis inflection. The method comprises the following steps: processing a chicken spleen tissue sample which serves as a test material, enriching methylation DNA, verifying, amplifying and purifying, and then sequencing; and identifying the gene taking an effect of methylation regulation in chicken salmonella enteritidis inflection by identifying gene methylation difference and analyzing gene functions and signal paths in accordance with change in a gene methylation difference multiple.

The present invention provides novel nucleic acids isolated from a cDNA library for fetal liver-spleen tissue, and the novel polypeptide sequences encoded by these nucleic acids. These novel polynucleotide and polypeptide sequences were determined to be a novel Interleukin-3.

The invention provides a tilapia feed additive for reducing proinflammatory response and enhancing immune response and a preparation method and an application thereof, and belongs to the technical field of aquaculture. The tilapia feed additive for reducing proinflammatory response and enhancing immune response comprises the following components in parts by weight: 25-35 parts of manyprickle acanthopanax stems, 15-25 parts of loquat leaves, 15-25 parts of tripterygium wilfordii, 10-20 parts of hawthorn fruits and 10-20 parts of mulberry leaves. The invention also provides a tilapia feed containing the tilapia feed additive, and an application of the tilapia feed additive or the tilapia feed in tilapia breeding. The application can effectively reduce the proinflammatory response of the headkidney tissue and the spleen tissue of the tilapia, and enhance the immune response capability of the tilapia, thereby improving the capability of the tilapia against streptococcal infection.

A Scophthalmus maximus rahbdovirus (SMRV0207, CCTCC No.202006) is prepared through sampling the liver, kidney, heart and spleen tissues from the scophthalmus maximus in ill, shearing, adding PBS, homogenizing, adding double antibody, freezing, thawing, centrifugal extracting, filtering, inoculating it to CLC 0207 cell, and constant-temp. continuous culture. Its advantages are high reproduction speed, and high reproduction valency up to 10 to the power 8 TCID 50 / ml. It can be used to prepare vaccine.

The invention provides a method for constructing a spleen tissue cell line of acipenser dabryanus. The method mainly comprises the following steps: (1), an MEM (minimum essential medium) culture solution containing fetal calf serum, human epidermal growth factors, human fibroblast-like cell growth factors and acipenser dabryanus serum and having the pH of 7.2-7.4 is prepared and stored in a refrigerator at the temperature of 4 DEG C for standby application; (2), spleen tissue is separated from the acipenser dabryanus body and subjected to primary culture with a tissue block culture method; (3), when the cell growth forms a single layer and the bottom coverage rate is higher than 70%, 0.25% trypsin digestion is adopted for subculture; (4), vigorously growing spleen cells with uniform forms are subjected to liquid nitrogen cryopreservation and resuscitaion culture. The spleen tissue cell line constructed with the method is fast to reproduce, has multiple forms, is fibroblast-like, and can realize continuous passage and cryopreservation resuscitation. The construction method is simple in technology, easy to operate, can be popularized to culture of spleen cells of other sturgeons and can be expected to be used for acipenser dabryanus species resource conservation as well as separation and identification of sturgeon diseases especially virus pathogens.

The invention relates to establish a detection method mode for hog cholera virus genetic recombination adenovirus carrier vaccine rabbit body effect. An SPF rabbit which is stable in production performance and quality serves as a carrier, after rAd-Eo-E2 immunization is subjected to a rabbit body for 14 days, swine fever spleen vaccine serves as strain used for counteracting toxic substances, after the toxic substances is counteracted, rabbit body heat of reaction is monitored continuously for 3 days, the protective efficacy of the vaccine is evaluated according to situation of forming heat (>= 40.5 DEG C) occurring on the rabbit body, and the detection method mode for the for rAd-Eo-E2 rabbit body efficacy is established. In the method establishing process, by means of temperature monitoring, an index ratio of the spleen to weight of the rabbit, spleen tissue section observation, RT-PCR detection and swine fever spleen gonorrhea separation and identification of an immune group and a matched group, whether the protective efficacy of the rAd-Eo-E2 immunization displayed by the result is accordant or not is determined, and evaluation conducted by adopting a relatively simple, convenient and easy detection method of temperature monitoring is determined finally.

The invention relates to a GSDMD inhibitor and application thereof in prevention and treatment of atherosclerosis and sepsis, and belongs to the technical field of biological medicines. GSDMD is a keymolecule for mediating cell scorch, and the cell scorch participates in occurrence and development of atherosclerosis and sepsis diseases. A polypeptide inhibitor is designed for the GSDMD, and the inhibitor can be combined with Caspase1 and Caspase4 / 5 / 11 to competitively inhibit the shearing effect of the enzymes on the GSDMD, so that the activation level of the GSDMD in vascular tissues of an atherosclerosis model mouse is remarkably inhibited, vascular inflammation is reduced, the development of atherosclerosis is inhibited, the activation level of the GSDMD in spleen tissues of a sepsis model mouse is remarkably reduced, the level of systemic inflammation is reduced, and the survival rate of the mouse is improved. The GSDMD polypeptide inhibitor can be used as a potential medicine forpreventing and treating atherosclerosis and sepsis.

The invention discloses a lipidomics analysis method of a blood deficiency mouse model, and belongs to the technical field of analysis of endogenous lipid substance change in the blood deficiency mouse body. The lipidomics analysis method comprises the following steps: ultrasonically crushing spleen tissues of the blood deficiency model mouse to be detected by using methanol, methyl tert-butyl ether and ultrapure water, centrifuging and concentrating an extracting solution, redissolving the extracting solution with methanol and isopropanol, carrying out filtering and sample feeding, using a liquid phase Waters ACQUITY UPLC HSS T3 chromatographic column for separation, using a mobile phase A and a mobile phase B composed of acetonitrile, water, ammonium formate, formic acid and isopropanolfor elution, using a full-scanning mode of a high-resolution mass spectrometer for detection in a positive ion mode and a negative ion mode respectively, carrying out qualitative and relative quantitative analysis on measured data according to a chemical formula of target lipid molecules. The method has the advantages of being sensitive, accurate and independent from standard compounds, and can beefficiently suitable for lipidomics analysis on the blood deficiency mouse model.

The invention provides a pig spleen transfer extracting method. The method has the characteristics that a high-pressure homogenizing machine is used for rapidly breaking spleen tissues and cells, a freezing step is not needed, and the production period can be more effectively shortened; the effective solid-liquid separation process is introduced after the homogenizing and before the filtering to demulsify the material and discharge gas in the material, so that the centrifugal difficulty is alleviated; the clear liquid supernatant is obtained through a centrifuging manner; the content of solids of the liquid filtered by a membrane can be obviously reduced, and the filtering rate and the service life of the membrane are increased; matched with the membrane filtering technology, the production period of the stock solution can be obviously shortened, convenience in operation is realized, the automation degree is high, and the large-scale production requirement can be met.

The Scophthalmus maximus rhabdovirus, SMV0207, with preservation number of CCTCC No. V202006, is prepared through taking liver, kidney, heart and spleen tissues of suffering Scophthalmus maximus, crushing, adding PBS for homogenation, adding amphitolerant, freezing, thawing, centrifugating to obtain coarse virus extracting liquid, filtering, inoculation to CLC0207 cell for further culture at constant temperature. The said process is simple and practical, and the virus strain may be amplified in CLC0207 cell fast in the amplification potency up to 108 TCID50 / ml. The virus strain may be used in preparing sea water fish rhabdovirus cell engineering vaccine.

The invention discloses a pig liver and spleen duct stem cell separation and three-dimensional organoid culture method. The method comprises two parts of dissociating pig liver and spleen tissue witha two-step method to obtain duct stem cells and carrying out three-dimensional culture on pig liver and spleen organoids. According to the three-dimensional organoid culture method based on pig liverand spleen duct stem cell separation, the technical problem of pig liver and spleen organoid establishment is solved, and the application of the organoid technology in animal nutrition research is promoted.

The invention relates to a fatty liver intelligent grading evaluation method based on abdominal CT, and relates to the field of intelligent medical image diagnosis. The method comprises the followingsteps: reading an abdominal CT image of a patient, selecting nearby four layers of slices corresponding to the maximum area of liver and spleen to construct a training sample set, and carrying out thepreprocessing of the data of the training sample set; constructing a UNet segmentation network model, sending the training sample to the segmentation network model for supervised learning, and aftertraining convergence of the segmentation model, using the model to segment CT slices to segment liver tissues and spleen tissues in the slices; respectively carrying out gridding cutting on liver tissues and spleen tissues to obtain a plurality of small rectangular areas with the same area, randomly selecting five small rectangular areas in the two tissues as sampling areas, calculating respectivegray average values as a liver CT value and a spleen CT value, and finally grading the fatty liver according to a liver / spleen CT ratio. According to the method, full-automatic segmentation of the liver tissue and the spleen tissue based on the abdominal CT image is realized, so that the fatty liver is intelligently graded and evaluated.

The invention discloses a method for detecting the tissue expression quantity of a heat stress protein HSP70 gene of Schizothorax wangchiachii. The method comprises the following steps of: firstly, extracting total RNA from the liver of Schizothorax wangchiachii, performing reverse transcription to obtain a cDNA first strand, cloning HSP70 gene 5'-and 3'-sequences by adopting a RACE amplificationmethod, and splicing partial sequences, and 5'-and 3'-sequences of HSP70 cDNA obtained by cloning by utilizing software. The obtained complete sequence contains 2348 bases, and the open reading frame(ORF) contains 1950 bases and encodes 649 amino acids. The expression quantity of Hsp70 in liver, kidney and spleen tissues is detected by fluorescence real-time quantitative PCR. The research of periodic starvation-feeding on HSP70 gene expression in liver, kidney and spleen of juvenile Schizothorax wangchiachii indicates that kidney can be used as the target organ for starvation stress. The method lays a foundation for researching the Hsp70 expression regulation mechanism of the Schizothorax wangchiachii.

The present invention relates to the cloning and preparing process and application of sow ablactation gene PSME1. Its preparing steps include computer aided cloning, extracting RNA of sow spleen tissue, RACE, cloning RACE resultant, sequencing to obtain total length of cDNA sequence, designing primer, amplifying genomic DNA sequence, testing the RFLP polymorphism, analyzing the association between RFLP polymorphism and ablactation, and testing the SSCP polymorphism.

The invention relates to a method for preparing concentration-controllable broiler spleen transfer factor oral solution, which belongs to the technical field of oral products. The method for preparing the concentration-controllable broiler spleen transfer factor oral solution comprises the following processes of: 1) cleaning, namely, cleaning the spleens of broilers and removing fat and capsules;2) crushing, namely, mixing the spleens with water according to the weight ratio of 0.5-3:1, and crushing the spleens by using a waring blender in a way of ensuring the fineness of homogenate; 3) repeated freeze thawing, namely, cooling spleen tissues at -15 to -30 DEG C for more than 10 hours, rapidly dissolving the cooled spleen tissues and repeating the freeze thawing for more than 3 times; and 4) dialysis, namely, placing the freeze thawing-treated spleen tissues into dialysis bags, sealing the dialysis bags, placing the sealed dialysis bags into a container filled with formaldehyde solution-containing normal saline, performing dialysis under magnetic stirring and performing dilution to obtain the clinically applied concentration-controllable broiler spleen transfer factor oral solution. The method has the advantages of simple process, economy, practicability, stable product performance, good clinical effects, wide application range and the like.

The invention relates to a new vascular tissue generation method. The method comprises, removing thoracic aorta, heart tissue, liver tissue, spleen tissue, lung tissue, kidney tissue and colon tissue from a mouse or a rat under a sterile condition; carrying out a combination of the removed tissues and the culture plate to generate the new vascular tissues respectively corresponding to the aorta, the heart tissue, the liver tissue, the spleen tissue, the lung tissue, the kidney tissue and the colon tissue. With the method provided by the present invention, disadvantages of cumbersome quantity and not high survival rate are overcome; a simple and convenient model of the new vascular tissue generating is established, wherein the model can be widely used; the method further can be applicable for screening studies of the tumor angiogenesis inhibitor.

The invention relates to a method for preparing a liposome solution of a chicken spleen transfer factor, belonging to the technical field of oral preparations. The method for preparing a liposome solution of the chicken spleen transfer factor comprises the following steps: 1, chicken transfer factor preparation process in which the spleen is cleaned, is crushed with water by a tissue triturator, and is frozen and thawed repeatedly for 3 to 8 times, the spleen tissue which is subjected to freezing and thawing treatment is filled in an analysis bag, and the sealed bag is put in a vessel filled with normal saline of a formaldehyde solution, and is stirred and analyzed by magnetic force to obtain the spleen transfer factor of the chicken; 2, liposome preparation process in which lecithin and cholesterol are put in an evaporative flask, vitamin E and chloroform are added, and the mixture is dissolved, decompressed, rotated and evaporated to form a liposome in a rotary flask; and 3, transfer factor liposome preparation process in which the chicken transfer factor is mixed with the liposome, and is homogenized with a homogenizer to prepare the nanometer chicken spleen transfer factor liposome. The method has the advantages of simple process, economy and practicality, stable performance, good clinical effect, wide application range and the like.

The invention discloses an application of hall crabapple flower polysaccharide in preparing an immunopotentiating drug. Cyclophosphamide is used to establish a low immunity model of a mouse to determine a spleen index, a thymus index, carbon granule clearance ability, the blood serum hemolysin content, and spleen lymphocyte proliferation ability of mice, the content of LDH (lactate dehydrogenase)and the content of ACP (acid phosphatase) in mouse blood serum and a spleen tissue, the content and mRNA (messenger ribonucleic acid) expression of cell factors (IL (interleukin)-2, IL-6, TNF (tumor necrosis factor)-alpha and IFN (interferon)-gamma), oxidative indexes in the mouse blood serum and the spleen tissue and the like; and test results indicate that the hall crabapple flower polysaccharide can effectively improve immunosuppression and oxidative stress caused by cyclophosphamide and strengthen immunocompetence of the mice, so that the hall crabapple flower polysaccharide can strengthenmouse immunity and be used for preparing the immunopotentiating drug.

The invention discloses application of endometrial stem cells in preparation of drugs for preventing or treating pulmonary fibrosis, and belongs to the technical field of biological medicine. The medicine provided by the invention comprises endometrial stem cells, and the solvent of the medicine is an aqueous solution of sodium chloride. The medicine provided by the invention can significantly improve pulmonary fibrosis, regulate the expression levels of alpha-SMA, collagen and IL-6 in lung tissue down, regulate the expression level of IL-10 in lung tissue up, and also regulate the expressionlevel of TNF-alpha in spleen tissue down.