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88 results about "Tissue morphology" patented technology

Tissue Morphology. Tissues can also have diverse morphologies, based on their function within an organism. Skeletal muscle cells form long bundles that are wrapped together. The bundles are connected to bones via tendons. These bundles are able to simultaneously contract to generate force.

Preserving fluid and preserving method for maintaining high cell activity of tissue sample

The invention provides a preserving fluid and a preserving method for maintaining high cell activity of a tissue sample, wherein the preserving fluid comprises the components: ion buffer solution components, carbohydrate components,, components avoiding the generation of ice crystals in and out solutions of tissue cells at a low temperature of 0-6 DEG C, components providing supplement, antioxidant and anti-apoptosis components for anabolism of the tissue cells; the preserving fluid has good biocompatibility, does not contain toxic or harmful components, and also does not contain components such as animal-derived protein, antibiotics and hormones influencing tissue gene expression. Different from traditional ultralow-temperature cryopreservation (-80 DEG C), the cryopreservation method disclosed by the invention can effectively realize low-temperature storage (0-6 DEG C) of fresh tissue samples of human beings and animals, maintains high cell activity of tissues, maintains tissue morphology, and is used for preservation and transportation of the tissue samples. Meanwhile, the nucleic acid integrity of the tissue and the stability of gene expression can be effectively maintained, the original state of the tissue is maintained, and the method can be used for gene detection, scientific research and other related experiments.
Owner:上海伯豪生物技术有限公司

High-carbon steel wire rod tissue grain size grading method

InactiveCN107202790AAssessment AchievementConsistent CorrosionPreparing sample for investigationMaterial analysis by optical meansChemical treatmentAlcohol
The invention discloses a high-carbon steel wire rod tissue grain size grading method. The method can finish high-carbon steel wire rod tissue grain size grading by the steps of manufacturing a sample, mechanically treating, chemically treating, observing and assessing and displaying results. The method disclosed by the invention achieves high-carbon steel wire rod tissue grain size grading by assessing the tissue grain size, namely the microscopy tissue morphology size. According to the method disclosed by the invention, an identical corrosion degree on the surface of a test sample is achieved by limiting nitric acid alcohol concentration and corrosion time; compared with a standard atlas, assessment errors can be reduced. According to the method disclosed by the invention, a supplementary effect among multiples is achieved by respectively grading tissue grade sizes under 100 times and 500 times. According to the method disclosed by the invention, four view fields and a core view field are chosen from on a detection ring of one fourth of the diameter of a high-carbon steel wire rod through observing positions and view field regulations; the choice of the observing positions and the view fields can reflect the tissue grain size situation of the whole cross section; thus, the obtained assessment results has strong representativeness, reality and validity.
Owner:南通市产品质量监督检验所

Organ vitrification freezing and preserving fluid

ActiveCN102550541AAvoid or reduce freezing damageImprove integrityDead animal preservationPolyethylene glycolGlycerol
The invention relates to the field of medical organ preservation and more particularly discloses a vitrification freezing and preserving fluid for preserving organs for a long term. The organ vitrification freezing and preserving fluid is characterized by comprising dimethyl sulfoxide, acetamide, glycerol, ethylene glycol, propylene glycol, cane sugar, polyethylene glycol and the like, wherein the concentrations of the dimethyl sulfoxide, the acetamide, the glycerol, the ethylene glycol, the propylene glycol and the cane sugar are 0.1-6 mol / L; the concentration of the polyethylene glycol is 1-20 mmol / L; and the pH value is in a range from 5.0 to 8.0. By implementing the vitrification preserving fluid for preserving organs, provided by the invention, the preserving fluid is in a glassy state rather than forming ice crystal in the cooling and freezing process to protect the tissue morphology and structure completeness, completely suppresses energy metabolism of tissue cells under a freezing condition and recovers biological functions of organs after anabiosis. The organ vitrification freezing and preserving fluid, disclosed by the invention, can be used for freezing and preserving kidney and also can be further widely applied to freezing and preserving of other organs for a long term for scientific research and clinic application.
Owner:潘峰 +1

Preparation and application of Nd<3-x>CoxNbO7-zincosilicate molecular sieve composite porous nanometer catalytic material

According to the invention, a powder catalytic material Nd<3-x>CoxNbO7 (x being greater than or equal to 0.5 and less than or equal to 1) is prepared by adopting a supercritical hydrosynthesis method and a chemical vapor condensation and deposition method; a composite porous nanometer catalytic material Nd<3-x>CoxNbO7 (x being greater than or equal to 0.5 and less than or equal to 1) -zincosilicate molecular sieve is prepared by adopting an impregnating and baking method; and a novel photoelectrode Nd<3-x>CoxNbO7 (x being greater than or equal to 0.5 and less than or equal to 1) is prepared. The three novel materials are represented: tissue morphology analysis is performed by a transmission electron microscopy, and results show that catalyst particles are irregular in shape, with the average particle size of 150 nm; phase analysis is performed by an X-ray diffractometer, and results show that Nd2CoNbO7 has a single phase, and relatively high crystallinity; the chemical speciation of the surface of the catalyst and the elementary composition of a microcell as well as the structural characteristics of an electronic shell are discussed by an X-ray photoelectron spectroscopy; and a characteristic absorption edge of the Nd2CoNbO7 is determined by a UV-Vis diffuse reflection spectroscopy to obtain the band gap width of the Nd2CoNbO7 which is 2.412 eV. Finally, the catalyst is used for decomposing water to produce hydrogen, and carrying out catalytic degradation on organic pollutants such as microcystic toxins, methylene blue and sulfamethoxazole in a water body under visible light. Experimental results show that the catalyst prepared according to the invention is good in catalytic effect.
Owner:NANJING UNIV

Experimental method and device for determining high temperature solidification phase transition rule

The invention relates to an experimental method and device for determining high temperature solidification phase transition rule, and belongs to detection instruments and meters. According to the invention, the solidification property of metal and alloy is determined at on-site cooling rate under a high-temperature condition so as to simulate real on-site working conditions; A phase transition temperature point is obtained by simulation determination of the high temperature solidification phase transition rule SPT; the whole phase transition process is observed; quantitative analysis of the phase transition is carried out; the phase transition rule is verified; and the transition rule is determined. According to the invention, a DTA differential thermal analyzer is used to determine the solidification phase transition point, liquidus line and solidus point of metal and alloy materials at different cooling rates; The cooling rate and the quenching temperature are determined based on the DTA results; with the method and device of the invention, a sample is melted and cooled to different quenching temperatures, and quenching is carried out by liquid nitrogen alcohol; the sample tissue morphology after quenching is observed; the contents of the first-precipitated phases in the high temperature tissue at different cooling rates are analyzed to obtain the high temperature solidification phase transition rule SPT of the sample; selection of an optimal solidification mode is realized at specific production requirements.
Owner:INNER MONGOLIA UNIV OF SCI & TECH

Method for preparing artificial muscle tendons by using biological three-dimensional printing and electrospinning technology

InactiveCN109701083AFast adhesionFast implementation of colonizationNon-woven fabricsProsthesisTissue morphologyEngineering
The present invention belongs to the technical field of biological three-dimensional printing and particularly discloses a method for preparing artificial muscle tendons by using a biological three-dimensional printing and an electrospinning technology. The preparation steps are as follows: S1, seed cell culturing; S2, nano fiber membrane preparing; S3, cell coating layer preparing; and S4, nano fiber membrane/cell coating layer treating. The biological three-dimensional printing and the electrospinning technology are combined, the electrospinning method is used to obtain directional nano fiber membranes, the biological three-dimensional printing technology is used for printing on the nano fiber membranes to obtain lubricating cell coating layers to rapidly realize adhesion and colonization of seed cells and obtain the high polymer material artificial muscle tendons containing the cells and having good lubricating performance, the manufacture method is simple and convenient, better simulates tissue morphology, cell composition and lubricating performance of the natural muscle tendon tissues, and is conducive to muscle tendon repair and normal function recovery.
Owner:SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE

Experimental method of effects of DNA oxidative damage and bone cell apoptosis on avascular necrosis

The invention discloses an experimental method of effects of DNA oxidative damage and bone cell apoptosis on avascular necrosis, the method comprises the following steps: experimental grouping and establishment of an animal model, preparation of specimens, histomorphology observation under a light microscope, observation by a transmission electron microscope, bone cell apoptosis situation detection by TUNEL (terminal dexynucleotidyl transferase(TdT)-mediated dUTP nick end labeling) method, bone marrow hematopoietic cell DNA oxidative damage detection by monoclonal antibody N45.1 immunohistochemistry method, and statistical analysis. According to the method, experiment animals are respectively killed in the second week and the fourth week after the last time of administration, and five animals are killed for each time in each group; bilateral femoral heads are taken for conventional fixed decalcification for stand-by testing, the number of vacant bone lacunas is counted by HE (hematoxylin-eosin) staining, the bone cell morphological change is observed by the electron microscope, the bone cell apoptosis situation is detected by the TUNEL method, the bone marrow hematopoietic cell DNA oxidative damage is detected by the immunohistochemistry method, and the relationship between SANFH ((steroid-induced avascular necrosis of femoral head)) and the bone marrow hematopoietic cell DNA oxidative damage and the bone cell apoptosis is explored, and provides new ideas for the further study of pathogenesis of the steroid induced avascular necrosis of femoral head.
Owner:刘万林

Continuous gradient bionic manufacturing method based on pneumatic accurate control active cartilage scaffold

The invention provides a continuous gradient bionic manufacturing method based on a pneumatic accurate control active cartilage scaffold. Based on sodium alginate SA ionic crosslinking and gelatin Gel low-temperature solidification forming mechanisms, SA, Gel, hydroxyapatite HA and cartilage cell suspension liquid are used as scaffold base materials, and according to the morphological characteristics of natural soft tissues, an extrusion deposition 3D printing technology based on high-pressure controllable gas is adopted to realize the preparation of an embedded living cell three-dimensional soft scaffold; and in the preparation process, the pressure applied to different charging barrels is dynamically and accurately regulated and controlled in real time, so that the accurate controllability of the component content is realized, then real-time dynamic mixing and continuous gradient laying of different component materials are realized through a dynamic mixing spray head, and the scaffold is crosslinked under the combined action of a CaCl2 solution and a low-temperature platform. The problems that an existing cartilage scaffold material is single in concentration, and the single material components of each layer in a layered structure do not conform to the anatomical characteristics of the continuous gradient of natural cartilage are solved.
Owner:NORTHWESTERN POLYTECHNICAL UNIV

Method for building psoriasis basic research models, and gas-liquid level transmembrane device

InactiveCN101570739APurification culture environmentGuaranteed observation cycle requirementsMicrobiological testing/measurementTissue cultureGas phaseBasic research
The invention provides a method for building psoriasis basic research models, and a gas-liquid level transmembrane device. The method utilizes a psoriasis streptococcal-induced mechanism and a T cell-mediated excessive proliferation mechanism, innovatively adopts a serum-free medium added with psoriasis peripheral blood T lymphocytes and hemolytic streptococcus antigens, and cultures psoriasis skin lesions in vitro. Characteristic tissue morphology of the psoriasis skin lesions can be maintained for as long as one week. The gas-liquid level transmembrane device used for culturing psoriasis in vitro skin lesions comprises the serum-free medium containing psoriasis peripheral blood T cells and the hemolytic streptococcus antigens in the method for building psoriasis basic research models, as well as sterilizing lens-wiping paper for manufacturing gas-liquid level, wherein tissue blocks and a gas phase are borne on the sterilizing lens-wiping paper; the gas phase is at 37 DEG C with 5 percent of CO2 saturation. The device not only can completely simulate gas-liquid junction environment for human skin growth, but also can reach the requirements of in vitro skin tissue culture. In addition, the device is easy to prepare, saves expenses, and has the advantages of repeatability, controllability and the like.
Owner:刘晓明 +1

organ vitrification cryopreservation solution

ActiveCN102550541BAvoid or reduce freezing damageImprove integrityDead animal preservationGlycerolPolyethylene glycol
The invention relates to the field of medical organ preservation and more particularly discloses a vitrification freezing and preserving fluid for preserving organs for a long term. The organ vitrification freezing and preserving fluid is characterized by comprising dimethyl sulfoxide, acetamide, glycerol, ethylene glycol, propylene glycol, cane sugar, polyethylene glycol and the like, wherein the concentrations of the dimethyl sulfoxide, the acetamide, the glycerol, the ethylene glycol, the propylene glycol and the cane sugar are 0.1-6 mol / L; the concentration of the polyethylene glycol is 1-20 mmol / L; and the pH value is in a range from 5.0 to 8.0. By implementing the vitrification preserving fluid for preserving organs, provided by the invention, the preserving fluid is in a glassy state rather than forming ice crystal in the cooling and freezing process to protect the tissue morphology and structure completeness, completely suppresses energy metabolism of tissue cells under a freezing condition and recovers biological functions of organs after anabiosis. The organ vitrification freezing and preserving fluid, disclosed by the invention, can be used for freezing and preserving kidney and also can be further widely applied to freezing and preserving of other organs for a long term for scientific research and clinic application.
Owner:潘峰 +1
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