38results about How to "Reliable experimental method" patented technology

The invention discloses a high-temperature high-pressure constant load stress corrosion experiment method and device. The technical scheme are that constant load stress corrosion experiment of metal materials can be conducted in certain stress state under the combined action of high-temperature high-pressure gaseous phase and liquid phase corrosion media, and subjected to combined action of the constant stress, and high-temperature high-pressure gaseous phase and liquid phase corrosion media in experiment process, and evaluation and research on the applicability and action mechanism of the metal material can be conducted under high-temperature high-pressure corrosive environment and tension stress state according to the experiment results. Damage to strength, plasticity and flexibility of the metal materials can be determined in high-temperature high-pressure gaseous phase and liquid phase corrosive environments under the action of any load by utilizing the experiment method and device, the mechanical property damage degree of the metal materials can be obtained in certain stress state and special corrosion environment according to the test result, thus conducting preference of the metal materials and applicability evaluation.

The invention provides a high-sensitivity method used for detecting and identifying 6 kinds of human coronavirus. The high-sensitivity method is based on exclusion of pollution caused by PCR amplification products, and is used for detecting and/or identifying human coronavirus by taking multiplex PCR-mass spectrometry as a platform. The 6 kinds of human coronavirus include HCoV-229E, HCoV-OC43, HCoV-NL63, HCoV-HKU1, SARS-CoV and MERS-CoV. Detection parts are the specific sequence domains in the genomes of the 6 kinds of human coronavirus. The high-sensitivity method is capable of realizing multiple gene detection of the 6 kinds of human coronavirus in a reaction, and increasing detectable rate; possesses advantages of high sensitivity and specificity, convenient operation, speediness, high throughput and the like; and can be used in scientific researches, clinical and epidemiological investigation.

The invention discloses a simulation experiment method for tunnel drivage blasting. The simulation experiment method includes the steps of: (1) manufacturing a simulation blasting experiment device, (2) utilizing the blasting experiment device to simulate tunnel cutting blasting, (3) utilizing millisecond initiation technology to perform blasting experiment, and (4) studying cutting holes, auxiliary holes, peripheral hole blasting rock breaking mechanisms, rock movement characteristics after the blasting and blasting effects. According to the simulation experiment method tunnel drivage blasting, a size design and a blasting parameter calculation design are performed through a law of similitude and a corresponding ratio of similitude and a blasting network is arranged, tunnel drivage blasting processes of various kinds of sections and processes of rock breaking and slag throwing of cutting drivage blasting are more completely and truly simulated, and a speed of blasting slag leaving cutting chamber is calculated according to law of motion of blasting slag. Accordingly, a more reliable experiment method and a more reliable experiment result are provided for a mechanism study of mine tunnel drivage blastings.

The invention discloses an electrochemical experiment apparatus and test method for corrosion with tensile stress loaded in high-temperature and high-pressure fluid environment. The apparatus comprises an autoclave, a branch pipe with circular flow and an electrochemical test system with tensile stress loaded; one side of the autoclave is connected to the branch pipe with circular flow, and the branch pipe with circular flow is provided with the electrochemical test system with tensile stress loaded. The apparatus and the test method can be used for researching corrosion behaviors of metal materials with the interaction of high temperature, high pressure, liquid phase fluid, loading of tensile stress, and other factors.

The invention discloses a method for testing a valve shell of a power station. A valve to be tested is connected with a testing pipeline; connection is set according to the testing requirement of the shell; firstly, the valve is heated to a set temperature which is between 37 and 700 DEG C; secondly, helium gas is filled in the valve; a shell test with gauge pressure not less than 1.5 times of nominal pressure is done; in a heat and pressure retaining state, the test is continued for the set time; and if the pressure value is reduced and exceeds the set standard, the shell is judged to be unqualified. A valve sealing test is similar to the method. The method uses helium gas as a medium and simulates that the high-temperature state of the valve in an actual working environment of the power station, thereby accurately measuring the quality situation of the valve and solving the technical problems which people hope to solve all the while and still do not succeed.

The invention discloses a method and a device for testing the environmental fracture toughness of a petroleum pipe. The method comprises the following steps of: detecting the size of the fracture toughness of the petroleum pipe under the service environment, processing the petroleum pipe into a sample, determining the experimental temperature, pressure and gas-liquid phase corrosive medium according to the service working condition of the pipe, loading the sample by the device for testing the environmental fracture toughness of the petroleum pipe, calculating the fracture toughness according to the load size and the crack expansion length after experiment, and obtaining stress-corrosion-cracking resistance, notch sensitivity and the action mechanism of the metal material in the service environment. The method and the device disclosed by the invention has the advantages that the size of the fracture toughness of the metal material of the petroleum pipe in any load and corrosion environment can be determined, the impact-toughness damage degree of the metal material in the service environment can be detected, so that the preference of the petroleum pipe is carried out and the experimental base is provided for designing the strength of the petroleum pipe.

The invention discloses a plasma free DNA methylation gene combination for identifying benign and malignant pulmonary nodules, and application of the plasma free DNA methylation gene combination. Theplasma free DNA methylation gene combination for identifying benign and malignant pulmonary nodules is selected from at least any two of SOX17, SCT, RASSF1A, SHOX2, HOXA7 and CDO1. The invention discloses application of the plasma free DNA methylation gene combination as a detection target in preparation of a reagent for identifying benign and malignant pulmonary nodules. According to the technical scheme in the invention, a methylation relative quantification method is adopted; the GAPDH is mainly used as an internal reference; a methylation relative quantification reference product is constructed; and then, a relative methylation standard curve is made. The methylation degree of a lung malignant tumour related gene can be obtained through a standard curve; compared with methylation quantitative detection, the gene combination is more sensitive; the detection accuracy is improved; the false positive rate is reduced; and the gene combination has more clinical value for diagnosis of benign and malignant pulmonary nodules.

The invention discloses a rapid molecule detection method of Pinus massoniana Lamb. and Pinus elliottii, and a specific primer pair thereof. The primer pair is PJ164L/R and has nucleotide sequences which are represented by SEQ. ID. No.1 and SEQ. ID. No.2 respectively in a sequence table, and the primer pair allows a 303bp product to be specifically amplified in Pinus massoniana Lamb. and a 325bp product to be specifically amplified in Pinus elliottii. The rapid molecule detection method of Pinus massoniana Lamb. and Pinus elliottii is established depended on the strong specificity characteristic of the sequences, allows determination to be carried out through the once implementation of a PCR amplification reaction and gel electrophoresis directly according to the fragment of the product, has the advantages of simple operation, accuracy, reliability and high sensitivity, is suitable for the rapid and reliable molecule detection and identification of Pinus massoniana Lamb. and Pinus elliottii, and has great scientific research values to identify the Pinus massoniana Lamb. and Pinus elliottii hybrid seedling in the future.

The invention discloses a water turning wheel type fatigue tester which comprises an L-shaped tester host, wherein the tester host is formed by connecting a horizontal base and a vertical support part; the support part is located at the left end of the base; the base of the tester host is horizontally provided with two strip-shaped convex edges along the length direction of the base; the tester host is provided with a transparent rectangular water tank, the bottom of which is located between the two strip-shaped convex edges. The water turning wheel type fatigue tester can embody the basic quintessence of the traditional swimming way, play the work principle of an advanced turning wheel type running machine, obtain a final fatigue exhaustion state without any traumatic driving way, and can save the animal resources to the maximum extent; such testing method is obviously more reliable, more real and more suitable for the large-sample tests. The water turning wheel type fatigue tester is controlled by a computer, has clear data, and is simple in structure, combined simply, cleaned rapidly, energy-saving and environment-friendly.

The invention provides a method for observing a morphological structure in mesophyll of a fossil plant. The method adopts means of immersing a fossil in distilled water, 40% hydrofluoric acid, Shu liquid and an alkaline solution successively, thereby removing impurities from the epidermis of the fossil; and then a mesophyll substance is separated independently through a sucker or a pipette for observation and identification. According to the method, the morphological observation and determination on the mesophyll in each experimental procedure are controlled to improve extraction of morphological character and function information of the plant, thereby providing reliable information materials for further identification on fossil plant leaves.

The invention discloses a non-coaxial bidirectional synchronous compression loading device and method. The device comprises a first height-adjustable loading platform and a second height-adjustable loading platform, wherein the first height-adjustable loading platform is provided with a first compression loading gun and a first waveguide rod, a second compression loading gun and a second waveguide rod are arranged on the second height-adjustable loading platform, a single-arm bending sample is arranged between the first waveguide rod and the second waveguide rod, the first compression loading gun generates a first compression stress wave and transmits the first compression stress wave to the first waveguide rod, the second compression loading gun generates a second compression stress wave and transmits the second compression stress wave to the second waveguide rod, the first compression stress wave and the second compression stress wave simultaneously reach the single-arm bending sample so as to achieve non-coaxial bidirectional synchronous dynamic loading, and the height of the first waveguide rod is different from that of the second waveguide rod. The device can be used for measuring the dynamic interlaminar fracture toughness of the composite material.

The present invention is the health food with ginger extractive and its use. Fresh ginger is crushed into coarse grains and thrice reflux extracted with distilled water to obtain extractive; the extractive is then alcohol precipitated and concentrated to obtain paste; the paste is mixed with proper amount of ginger powder; the mixture is pelletized while adding alcohol and dried at 60-70 deg.c; and the pill is cooled and added with magnesium stearate in 0.05-0.1 wt% before pressed into tablet or capsulized into capsule preparation. The extractive has the functions of regulating and lowering blood fat and thus can prevent hypertension, atherosclerosis and coronary heart disease.

The invention provides a method for detecting and identifying human polyomavirus with the high sensitivity. According to the method, elimination of PCR (polymerase chain reaction) amplification product pollution is used as a precondition, and multiplex PCR-mass spectrometry is used as a platform to detect and/or identify the human polyomavirus with the high sensitivity. 12 kinds of human polyomaviruses detected by the method include BKPyV (BK polyomavirus), JCPyV (JC polyomavirus), KIPyV (Ki polyomavirus), WUPyV (WU polyomavirus), MCPyV (merkel cell polyomavirus), HPyV6 (human polyomavirus6), HPyV7 (human polyomavirus7), TSPyV (trichodysplasia spinulosa-associated polyomavirus), HPyV9 (human polyomavirus9), MWPyV (MW polyomavirus), STLPyV (STL polyomavirus) and HPyV12 (human polyomavirus12), and a detection part is a late coding region VP1 of coding virus capsid protein in a virus genome.

The invention relates to a coal seam hydraulic punching simulation experiment method. An experiment device comprises a coal pressing column head, a coal storage cylinder body, a press machine, an experiment pump machine, an electric coal drill, an experiment gas cylinder, a pressure sensor and a pressure detector, the coal pressing column head is arranged above the coal storage cylinder body, and a pressing head on the lower part of the coal pressing column head is matched with an inner cavity of the coal storage cylinder body; a press machine is arranged on the coal pressing column head, an inflation pipe is arranged on the side wall of the coal storage cylinder body, the inflation pipe can be connected with an experiment gas cylinder to inflate the coal storage cylinder body, the electric coal drill can drill a simulated coal seam in the coal storage cylinder body, and the experiment pump machine can perform a hydraulic punching experiment on the coal seam after hole forming. The pressure detector is connected with the pressure sensor arranged in the coal seam and monitors the pressure of each layer in real time. The coal seam hydraulic punching process is reasonably simulated, a reliable experimental device and an experimental method are provided for subsequent experiments, and reliable theoretical basis and method measures are provided for actual construction.

The invention discloses a natural stress measuring device. The device comprises a flat jack, a hydraulic system, a deformation monitoring system and a data analysis system; the flat jack is used for applying expansive force to a pre-excavated flat cave, and pressure sensors are mounted at the two sides of the flat cave; the hydraulic system is connected with the flat jack and used for providing hydraulic pressure for the flat jack; the deformation monitoring system is electrically connected with the hydraulic system, the deformation monitoring system is used for measuring the distance between two preset monitoring points, and the hydraulic system can adjust the hydraulic pressure according to the distance measured by the deformation monitoring system; and the data analysis system is electrically connected with the two pressure sensors and is used for calculating the magnitude of the natural stress according to pressure values collected by the two pressure sensors when the distance between the two monitoring points recovers to an initial value. According to the invention, the problem that the current natural stress measurement method cannot meet the technical requirements of rock natural stress test in a complex stress state is solved.