Method for detecting human intestinal virus with high sensitivity

A human enterovirus, enterovirus technology, applied in microorganism-based methods, biochemical equipment and methods, microbial assay/test, etc., can solve false negatives, lack of simultaneous detection of multiple human enteroviruses, sensitivity lower problem

Inactive Publication Date: 2015-01-14
INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The limitations of existing methods mainly include several aspects: lack of a comprehensive method for simultaneous detection of multiple human enteroviruses; reverse transcription polymerase chain reaction method relies on sequencing and has poor specificity; low sensitivity and prone to false negatives Wait

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  • Method for detecting human intestinal virus with high sensitivity
  • Method for detecting human intestinal virus with high sensitivity

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Embodiment Construction

[0043] The invention includes simultaneous detection of one or more of 21 known human enteroviruses, each genotype adopts VP1 gene region for detection. The specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0044] According to the method for highly sensitive detection and / or identification of human enteroviruses provided by the embodiments of the present invention, 21 kinds of human enteroviruses to be detected include: Coxsackievirus group A (CVA) 2, 4, 5, 6, 8, 9 , 10, 12, 16 types (A, B subtypes), Coxsackie virus group B 3, 4, 5 types, enterovirus type 71 (A, B, C subtypes), echovirus 1, 4, 7, 19, 25 types. . Including the following steps:

[0045] (1) According to the full sequence of the human enterovirus to be tested, obtain the VP1 gene region, select the specific conserved sequence of each type, and design a pair of PCR primers with 10 bases (ACGTTGGATG) at the 5' end of the primers Any combina...

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Abstract

The invention relates to a detection method of pathogenic microorganism, and in particular relates to a method for detecting human intestinal virus related to hand-foot-and-mouth disease with high sensitivity. The method is realized through a detecting kit which comprises polynucleotide as shown in SEQ ID No.1-SEQ ID No.75.

Description

technical field [0001] The invention relates to a method for detecting pathogenic microorganisms, in particular to a method for detecting human enteroviruses related to hand, foot and mouth disease with high sensitivity. Background technique [0002] Human enterovirus (HEV) belongs to Picornaviridae, including poliovirus, coxsackievirus group A and group B (CVA and CVB), echovirus (E), new enterovirus, etc. More than 100 types. Human enteroviruses can cause polio, hand, foot and mouth disease, herpetic angina, acute hemorrhagic conjunctivitis, etc. [0003] Hand foot and mouth disease (HFMD) is a worldwide widespread infectious disease, mostly occurs in children under the age of 5, can cause herpes on the hands, feet, mouth and other parts, a small number of children can cause myocarditis, pulmonary Edema, aseptic meningoencephalitis and other complications. Severe cases have a higher mortality rate. In 2008, my country included it as a legal infectious disease for manag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
Inventor 彭俊平郭军华金奇
Owner INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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