250 results about "Pinus massoniana" patented technology

The invention relates to a method for inducing an embryonal-suspensor mass (ESM) regeneration plant from an immature seed of pinus massoniana. By using the characteristic of continuous schizogenesis capability of the ESM in the immature seed of the pinus massoniana, solid culture and liquid culture are performed alternatively and ESM forms a completely-grown embryo with a normal structure after a maintaining stage, a multiplication stage, an embryo pre-mature stage and a maturation culture stage, so that a pinus massoniana plant is obtained by germinating and culturing the embryo. Due to the adoption of the method, a large number of regeneration plants of a pinus massoniana embryo of which the hereditary basis is accordant can be obtained and new technology with a short period and high occurring efficiency is provided for the storage of pinus massoniana rare generic resource and the mass production of excellent-gene nursery-grown plants.

The invention discloses a carboxylated alkali lignin sulphonate dye dispersant and a preparation method. The reaction raw materials comprises the following formula by mass fraction: 100 parts of alkali lignin, 20-60 parts of sulfonating agent, 2-20 parts of aldehydes compound and 20-45 parts of carboxylic acid compound. The alkali lignin is prepared by acidification of bamboo, Chinese oak, poplar, Eucalyptus, birch, a papermaking black liquor by a masson pine alkali method, the mass percentage is 95%. The preparation method comprises the following steps: preparing alkali lignin to a solution with mass fraction of 20-40%, then adding the sulfonating agent and the aldehydes compound for sulfomethylation reaction to prepare alkali lignin sulphonate, and the alkali lignin sulphonate is subjected to a carboxylation reaction with the carboxylic acid compound to prepare the carboxylated alkali lignin sulphonate. The prepared carboxylated alkali lignin sulphonate contains sulfonic group and carboxyl group, can be used as the dye dispersant, and has good dispersibility to the disperse dyes. Phenolic hydroxyl group with reductibility is subjected to the reaction in a modification reaction, so that reduction to an azo dye and contamination to the dispersant can be greatly reduced.

The invention relates to a method for producing homogenous carbonized wood by using pinus massoniana wood as a raw material, and belongs to the technical field of processing of forestry products. The method is implemented through processes of degreasing, carbonizing and the like. The method has the benefits that the pinus massoniana wood is degreased, so the phenomena of non-uniform internal and external carbonization degree caused by excessive external carbonation at high temperature when excessive grease is overflown in a carbonization process can be avoided, and foundation is laid for homogenous carbonization; different heating and cooling speed, carbonization temperature and carbonization time are adopted according to different materials and colors of the pinus massoniana raw materials, so that each part of the pinus massoniana wood can be subjected to carbonization with the same degree from outside to inside, moisture absorptivity, dimensional stability, mechanical property, corrosion resistance, hardness, pressure resistance, gluing and surface coating performance, particularly color of each part show the same good characteristic, the true homogenous effect is achieved, the application field of the pinus massoniana wood is greatly enlarged, and the application value and the economic benefit are improved.

The invention discloses a method for reforming a closed pure pinus massoniana forest into a mixed forest. The method comprises the following steps: selecting proper mixing tree species from the closed pure pinus massoniana forest according to retention density after intermediate cutting and biological characteristics of the mixing tree species, and planting 1-5 mixing tree species randomly according to a certain ratio. The method aims to form a long-term pinus massoniana artificial mixed near-natural forest with both ecological and economic functions, and on the basis of the time that a young pinus massoniana forest, a middle-aged pinus massoniana forest and a near-mature pinus massoniana forest are subjected to intermediate cutting for multiple times, and the sufficient consideration of the biological characteristics of the mixing tree species, proper mixing tree species with economic values can be reasonably selected. Practical applications show that the method is suitable for reforming the pure pinus massoniana forest into the mixed forest after closing under conditions of different tree ages and environments, the efficiency in artificially constructing the mixed forest can be remarkably improved, the mixed forest construction time can be shortened, the planting cost of mixed forest construction can be reduced, and due to multiple tree species and long and short-term planting, the economic benefits and the ecological benefits of the mixed forest can be sufficiently brought into play.

The invention discloses an ex-vitro rooting method for tissue culture seedlings of pinus massoniana. The ex-vitro rooting method comprises the following steps: taking terminal buds of aseptic seedlings with 0.5cm long hypocotyls as explants for inducing cluster buds in a culture medium I; carrying out elongation culture on the induced cluster buds in a culture medium II, individually cutting off the terminal buds, and inoculating the cut terminal buds into a culture medium III for propagation to obtain subculture buds I; repeatedly elongating the subculture buds I, individually cutting off the subculture buds I, dividing the cut subculture buds I into terminal buds and bud sections, continuously propagating in a subculture propagation culture medium III to obtain subculture buds II, and repeatedly elongating and propagating the subculture buds II to obtain new propagation subculture buds; and transplanting the individually cut 2.5-3.5cm high subculture buds into a seedling culture cup for ex-vitro rooting, and transplanting the ex-vitro subculture buds into a seedling nursery for culture to obtain the ex-vitro tissue culture seedlings. According to the method disclosed by the invention, the production program of test-tube seedlings is simplified, the production cost is reduced, the production efficiency is improved, the rooting treatment time is 25-30 days, the ex-vitro rooting rate reaches 92%-98%, the root system quality is higher, the transplanting survival rate reaches 90%-95%, and the method has better economic, social and ecologic benefits.

The invention discloses an artificial pollination breeding method of a pinus massoniana hybrid. The artificial pollination breeding method comprises the following steps of 1, selection of masson pine as a female parent, 2, selection of pinus elliottii, pinus elliottii-pinus caribaea hybrid, pinus caribaea and pine moth-resistant pinus massoniana as male parents, 3, collection of male parent pollen, 4, treatment on the male parent pollen, 5, bagging and artificial pollination by a home-made pollination tool, and 6, cultivation. Through the artificial pollination breeding method, seeds of the pinus massoniana hybrid having the excellent and stable properties of straight trunk, fast early-stage growth rate, high rosin yield and pest and disease resistance are obtained. The artificial pollination breeding method solves the problems of open pollination in a pinus massoniana seed garden and improves crossbreeding efficiency. The artificial pollination breeding method is a complete artificial pollination breeding method, has simple processes, is practical, has a low cost, low requirements on operators, and small influences by a natural environmental factor such as weather, can effectively improve cross pollination efficiency in a pinus massoniana seed garden, can improve accuracy and reliability of cross pollination, can accelerate seed selection of a novel forestall germplasm resource variety, and has good economic, social and ecological benefits.

The invention relates to a method for intermediate cutting growth of pinus massoniana. The method particularly comprises that: for a new afforesting land, the optimal silviculture density is 3,100 to 3,300 plants/hectare, the trees of which the stand age is 16 years undergo 20 to 30 percent of intermediate cutting for the first time, and the trees of which the stand age is 20 years undergo 20 to 30 percent of intermediate cutting for the second time; and the trees of which the stand age is more than 26 years stop growing and are at the over-mature stage, and cut at the time, and the forestry land is replanted. For the planted forestry site, the pinus massoniana of which the stand age is 16 years uses different intermediate cutting strengths of between 20 and 51 percent: namely the forest stand of which the density is 2,445 to 3,210 plants/hectare uses the intermediate cutting strengths of between 20 and 29 percent, the forest stand of which the density is 3,210 to 3,990 plants/hectare uses the intermediate cutting strength of between 30 and 39 percent, the forest stand of which the density is more than 4,590 plants/hectare uses the intermediate cutting strength of between 40 and 51 percent, the trees of which the stand age is 20 years can undergo 20 to 30 percent intermediate cutting for the second time, the trees of which the stand age is more than 26 years stop growing and are at the over-mature stage, and cut at the time, and the forestry land is replanted. The method has the advantages of keeping the self control ability, biological diversity and productivity of forest land of the forest ecosystem and increasing the timber output.

The invention belongs to the technical field of health care food processing and relates to a new preparation of a Ficus carica Linn health care product which is prepared by the raw materials of Ficus carica Linn, Dioscorea opposita Thunb, Rhodiola sachalinensis A.Bor., Glycine max(L.)Merr., Hippophae rhamnoides L., pine pollen (the pure and dry pollen of Pinus Massoniana Lamb. and Pinus tabulaeformis Carr.), Ziziphus jujuba Mill.var.inermis(Bunge)Rehd., maltitol and the like. Glycine max(L.)Merr., Rhodiola sachalinensis A.Bor. and Ziziphus jujuba Mill.var.inermis(Bunge)Rehd are boiled in water for extracting biological active ingredients, Ficus carica Linn and Hippophae rhamnoides L. are subject to juice squeezing, Glycine max(L.)Merr. is soaked and prepared into cooked soybean milk which is spray-dried to obtain bean powder, and the wall of pine pollen is broken by being ground on a high-pressure micro-powder pulverizing mill. The preparation can keep the content of relevant biological active ingredients of Ficus carica Linn and other raw materials, is suitable for industrial production, has low cost and reliable quality, is applicable to various groups, is suitable for being taken with water at breakfast and can achieve the function of health care to the human body. Moreover, by adopting the invention, the varieties of the products prepared by Ficus carica Linn are increased and the problems of storing and transporting the fresh fruits of Ficus carica Linn are solved.

The invention discloses a stress-resistance and health-care mycorhiza microbial inoculum of Pinus massoniana and an application method of the stress-resistance and health-care mycorhiza microbial inoculum. The stress-resistance and health-care mycorhiza microbial inoculum is prepared by compounding a mycorhiza microbial inoculum and plant hormone naphthylacetic acid or benzpyrole-3-acetic acid; and the mycorhiza microbial inoculum is formed by fermenting Lactarius akahatsu or Lactarius hatsudake through a solid fermentation culture medium. The stress-resistance and health-care mycorhiza microbial inoculum is used for carrying out slurry dipping treatment on seedling root systems or is buried into Pinus massoniana forest soil in a forestation process, so that not only can root-taking and growth of the Pinus massoniana be increased, but also the tree vigor is enhanced and the stress resistance is improved; and therefore Pinus massoniana defoliation, seedling damping-off and Pestalotiopsis apiculatus are prevented from happening.

The invention discloses a method for shortening building time of a garden of Masson's pine clone graft seeds. According to the characteristic that slash pine is superior to Masson's pine in early growth, stocks of slash pine and Masson's pine are planted in a manner of two plantlets in one hole, and slash pine is grafted to Masson's pine in autumn and is supplementarily grafted in spring. Therefore, garden building time is shortened effectively while guaranteeing building quality, and the building efficiency of the Masson's pine clone graft seed garden is improved. The seed garden can be built for only 14 months by the method, which is 8 months smaller than the traditional building time. The efficient building method is economical, efficient, simple to operate, applicable to construction of bases for fine Masson's breeds and popularization to base production institutions, and practically significant to building of fine bases for Masson's pine.

The invention discloses a tissue culture and regenerated plant in vitro mycorrhization method for pinus massoniana, which belongs to the technical field of biotechnology and modern forestry. The method comprises the following steps of: taking a terminal bud of an aseptic seedling of the pinus massoniana as an explant, inducing to produce a large quantity of multiple shoots with strong growth first, further inducing the generation of an adventitious root, transferring a plant with the macroscopic adventitious root onto a perlite substrate under the condition of asepsis after the induction of the adventitious root is finished, and inoculating ectomycorrhizal rungi Pisolithustinctorius at the same time to realize the mycorrhization in a bottle. The mycorrhization improves the quality of a root system of the adventitious root of a tissue culture regenerated plant of the pinus massoniana, and ensures that the average lateral root number and the average root length are greatly improved. The mycorrhization also makes the state of the adventitious root greatly changed, and a fungal mantle obtained by closely arranging more than ten layers of mycelia is formed on the surface of the adventitious root. Through the method, the induction rate of the multiple shoots of the pinus massoniana reaches 95 percent, the proliferation rate reaches 430 percent, the rooting rate reaches 85 percent, and the transplantation survival rate is improved to more than 85 percent from 65 percent.

The invention discloses a method for preparation of a pinus massoniana lamb superior individual sterile explant and induction of an initial bud. The method comprises the following steps: grafting by selecting a sprout twig with a newly grown tender top in the current year of an adult superior individual as a scion and taking a pinus massoniana lamb tissue culture seedling as a stock, performing disinfection treatment on the sprout twig by adopting a series of disinfectants to match for disinfection, and putting the explant scion in an optimized induction culture medium to perform initial bud induction to obtain the sterile explant and the pinus massoniana lamb initial bud with rapid growth and vigorous vitality. By adopting the method disclosed by the invention, the technical problems of difficulty in disinfection of the explant, browning, low incidence of the initial bud, poor activity of a bud shoot and the like in tissue culture of the pinus massoniana lamb adult superior individual are solved, the incidence of the initial bud and the activity of the bud shoot are improved, the robust explant which is sterile above 90% can be obtained, and better economic benefits and social benefits are obtained.

The invention discloses a twig and short shoot cutting seedling raising method for pinus massoniana tissue culture seedlings. The method includes the working procedures of selecting a container, preparing a matrix, collecting cutting shoots, treating the cutting shoots, conducting cuttage and managing a nursery; the suitable seedling raising container is selected, after the matrix is prepared according to a certain ratio and disinfected through a disinfecting method, juvenile-form twigs are collected to serve as the cutting shoots to be eluted and disinfected, after the cutting shoots are treated with a root growth promoting agent, cuttage is directly carried out on the cutting shoots, control over light, temperature, water and fertilizer conditions is enhanced in the period, the cutting shoots take roots about a month later, the rooting rate reaches 98% or above, and the survival rate reaches 97% or above. The pinus massoniana tissue culture seedlings serve as cutting shoot stock plants, the problems that the tissue culture subculture period is long, seedling raising cost is high are solved, the propagation rate is large, the number of qualified juvenile-form twigs for cottage is large, cutting rooting is easy, the effect is remarkable, and the seedling raising method has good economic benefits, social benefits and ecological benefits.

The invention discloses a method for determining a first intermediate cutting age of a made forest. The first intermediate cutting time of the made forest is determined by the ratios of the average distances among trees in the made forest to crown diameters and inflection points of curvilinear equations for ages. For a Chinese fir made forest, namely the Chinese fir made forest with the density of plantation of 1,667, 3,333, 5,000, 6,667 and 10,000 plants/hm<2> respectively, the first intermediate cutting time is 14 years, 10 years, 9 years, 7 years and 6 years respectively; and for a pinus massoniana made forest, namely the pinus massoniana made forest with the density of plantation of 1,667, 3,333, 5,000 and 6,667 plants/hm<2> respectively, the first intermediate cutting time is 9 years, 8 years, 7 years and 6 years respectively. By the method, the calculation is simple, and the first intermediate cutting age can be directly determined by the density of plantation.

The invention discloses a forestation method improving the fir wood property. The method comprises the following steps of high-quality seedling cultivation, land preparation and fertilization, tree species arrangement and planting and tending management. By conducting mixed plantation between plants or mixed plantation by rows on fir and one or more of pinus massoniana, phoebe bournei, steamed beech, liriodendron chinese and schima superba, competition between the fir and other tree species is increased, broad leaves of the phoebe bournei, the steamed beech, the schima superba, the liriodendron chinese and the like are likely to rot to form humus compared with Chinese fir leaves, multiple mineral nutrition is contained, the mineral nutrition is absorbed by the fir and then accumulated in wood of the trunk, and the wood property of the fir is improved.

The invention relates to a seedling raising method using peat containers capable of increasing the survival rate of Pinus massoniana, which is characterized by including the steps: firstly, using nonwoven seedling containers as peat containers; secondly, using peat, vermiculite and garden soil as a culture medium; thirdly, preparing soil to make a bed; fourthly, soaking seeds in warm water, sterilizing and drying in the shade after washing; fifthly, sowing seeds into each peat container, covering with screened fine soil after sowing, covering with leaves of Pinus massoniana, and watering the medium thoroughly; sixthly, watering every day to maintain the leaves of Pinus massoniana humid before emergency of seedlings; removing the leaves of Pinus massoniana when the seeds are emergent 10-12 days later followed by sowing and the emergence rate of the seedlings reaches 60-80%; stopping watering 60-80 days later followed by sowing; and investigating seedling height, stem thickness and survival rate 90-100 days later followed by sowing, and obtaining qualified seedlings 10-12cm in height. The vermiculite, the garden soil and the peat are used as the medium, and the lightweight medium good in ventilation is beneficial to growth and development of seedling root systems.

The invention relates to a bio-pharmaceuticals production technology, and in particular relates to a method for improving poria cocos mycelium protein content and liquid fermentation biomass. The method comprises the following steps: (1) preparing a poria cocos basidiospore suspension liquid; (2) mutating the poria cocos basidiospore through Co60-gamma radiation; (3) pairing basidiospore strains; (4) inoculating a pinus massoniana steep solid culture medium bevel, wherein the culture medium is composed of pinus massoniana extractive, potato extractive and glucose; and (5) inoculating a pinus massoniana extractive liquid fermentation culture medium, wherein the culture medium is composed of pinus massoniana extractive, microcrystal fiber, glucose, glycerinum, ammonium sulfate, magnesium sulfate, potassium hydroxide and phosphoric acid. The poria cocos strain with high mycelium protein content is firstly obtained through mutation, the adopted pinus massoniana steep solid culture medium bevel is in favor of the rapid growth of the poria cocos mycelium in comparison with the potato glucose culture medium, and the adopted pinus massoniana extractive liquid fermentation culture medium is in favor of obtaining high biomass.

The invention discloses a method for identifying germplasm of pinus massoniana by utilizing an SSR (simple sequence repeat) molecular marker based on transcriptome sequencing. The method is characterized by comprising steps as follows: (1) a Tiangen DNA secure Plant Kit optimization step is adopted for DNA extraction, and a pinus massoniana sample is amplified by the aid of a primer according to a PCR (polymerase chain reaction) system and a reaction procedure; (2) an SSR primer is designed on the basis of the sequence for transcriptome sequencing of pinus massoniana, and a PCR amplification system is optimized; (3) finally, the PCR primer is detected with a silver staining method through optimized 10% polyacrylamide denaturing gel electrophoresis, and a result is judged according to existence and the magnitude of a band.

The invention discloses a method for building a cutting orchard with Pinus massoniana Lamb. tissue culture seedlings. The method comprises the procedures of orchard selection, soil preparation, nursery stock preparation and field planting, annual management and cutting orchard management after field planting. The method comprises the following specific steps: selecting an orchard and performing soil preparation for later use; performing tissue culture on germinating robust Pinus massoniana Lamb. free from plant diseases and insect pests, and planting in a seed bed; after field planting, performing water and fertilizer management the same year; and performing fertilization and plant disease and insect pest prevention and control management on the cutting orchard to obtain scions which grow robustly and are super-vital, uniform in thicknesses and easy for cutting survival. The technical problems of poor cost of the current Pinus massoniana Lamb. tissue culture seedlings, poor rejuvenation function of stock plants in cutting rapid propagation, quick physiologic aging of the scions, poor vitality, difficulty for cutting survival and the like are solved. The method for building the cutting orchard with Pinus massoniana Lamb. tissue culture seedlings has the advantages of short stock plant culture period, high cutting survival rate, and good economical benefit, social benefit and ecological benefit.

The invention discloses a high-efficiency phosphate-solubilizing penicillium and application thereof in promoting the growth of Chinese red pines. The classification designation of the high-efficiency phosphate-solubilizing penicillium is Penicillium pinophilum JP-NJ4; and the Penicillium pinophilum JP-NJ4 was collected at China Center for Type Culture Collection in Wuhan university, Wuhan, China on May 14, 2012, and the collection number is CCTCC NO:M2012167. The Penicillium pinophilum JP-NJ4 has the following advantages: the Penicillium pinophilum JP-NJ4 has high solubility on insoluble inorganic phosphate such as tricalcium phosphate (Ca3(PO4)2), aluminum phosphate (AlPO4) and calcium hydrogen phosphate (CaHPO4.2H2O); and pot incubation tests verify that the growth of Chinese red pines can be obviously promoted after Chinese red pine seedlings are inoculated with the JP-NJ4 microbial inoculum. Thus, the Penicillium pinophilum JP-NJ4 can provide an excellent strain resource for the development of a microbial bacterial fertilizer, and has favorable application development prospects.

The invention relates to the technical field of insect pest prevention and treatment in forestry, in particular to a method for comprehensively preventing and treating longhorn beetles. In the method,first, healthy masson pine wood is selected, pine wood nematodes are introduced and utilized for infecting the masson pine, then the infected masson pine and beauveria bassiana are blended and subjected to anaerobic digestion, yeast is obtained, monoterpene pheromones of the pine wood nematodes are carried in the masson pine yeast after infection of the pine wood nematodes, the pine wood nematodes are hosts of the longhorn beetles, the pheromones of the hosts will lure and gather the longhorn beetles, the lured and gathered longhorn beetles will make contact with beauveria bassiana, in this way, the longhorn beetles and larvae of the longhorn beetles can be effectively killed, the longhorn beetles will also carry the deadly beauveria bassiana to other places to promote diffusion of the longhorn beetle deadly bacteria, and then the killing rate is further increased for the longhorn beetles. Sclerodermus harmandi as parasitical natural enemies of the longhorn beetles is released aroundluring wood filled with self-made luring and killing agents and is parasitic on the larvae of the longhorn beetles to further increase the killing rate for the longhorn beetles, finally, the luring wood is burned down in a concentrated mode, the longhorn beetles are thoroughly killed, and broad application prospects are achieved.

The invention discloses a liquid fertilizer for promoting rooting of pinus massoniana softwood cutting. The liquid fertilizer comprises the following raw materials: potassium nitrate, ammonium sulfate, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, boric acid, copper sulphate, nicotinic acid, folic acid, VB12, biotin, m-trihydroxybenzene, choline chloride, indolebutyric acid, ABT1 and brassinolide. A preparation method of the liquid fertilizer comprises weighing potassium nitrate, ammonium sulfate, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, boric acid and copper sulphate, dissolving the weighed materials in water, adding the obtained product into a reactor, and performing uniform stirring to obtain a mixed solution I; and adding nicotinic acid, folic acid, VB12, and biotin one by one into the mixed solution I, adding m-trihydroxybenzene, choline chloride, indolebutyric acid, ABT1 and brassinolide in sequence, and performing cooling after the reaction is over to obtain a mixed solution II. Root-promoting agents, such as vitamins, phenolic acid substances and plant growth regulators, match a large number of microelements, thereby increasing the rooting rate of pinus massoniana cutting. The liquid fertilizer has great economic, social and ecological benefit.

The invention discloses a pinus massoniana cpSSR polymorphism primer and an identification method of pine sibling species of the primer. According to design of a chloroplast genome of pinus massoniana, 16 pairs of cpSSR primers are obtained, further, 7 pairs of primers which are stable in difference and good in polymorphism are screened from the 16 pairs of cpSSR primers, and through utilization of the 7 pairs of primers, a finger print and phylogenetic tree of 8 kinds of pine sibling species are successfully established. Based on the 7 pairs of cpSSR primers, a PCR amplification reaction is carried out. Capillary electrophoresis technology is used to analyze a PCR product, 8 kinds of pine sibling species trees can be accurately identified. The primer and method are efficient, accurate, and convenient and are not prone to be affected by the environment. The polymorphism primer and an identification method thereof can reflect the genetic information of the 8 kinds of pine plasmons, andcan be used for research of identification of 8 kinds of pine sibling species and genetic relationship.

The invention relates to a preparing method of a masson pine seedling culture medium. The preparing process includes the steps that 50-60 parts of waste snake house packing, 25-40 parts of chinese mesona herb residues, 10-25 parts of manioc residues, 10-20 parts of straw pellets, 5-10 parts of ramulus mori pellets, 3-8 parts of cob residue pellets and 3-5 parts of bagasse pellets are weighed by weight; the raw materials are mixed uniformly to obtain a premix compound; mixed bacteria are added into the premix compound; the premix compound and the mixed bacteria are mixed uniformly and then putinto a tunnel fermenting kiln to conduct fermentation; pasteurization is conducted on the premix compound obtained after fermentation to obtain culture material; the culture material obtained after pasteurization is placed in a edible fungi cultivation shed to be cooled to 25 DEG C or below, edible fungus strains are planted to conduct edible fungus cultivation; after edible fungus cultivation isfinished, masson pine branch and leaf pellets are added, the mixture is placed for one week or more and turned then to obtain the masson pine seedling culture medium. Masson pines cultivated by the seedling culture medium are high in survival rate and have developed lateral and fibrous roots.

The invention discloses a method for storing and unfreezing pinus massoniana pollen. The method comprises the following steps of: storing, namely performing water culture on pinus massoniana male flowers with branches and leaves, picking off buds when the buds is to blossom, placing the buds into a kraft paper envelope, drying at constant temperature, dispersing pollen, pinking one corner of the envelope by using a needle, collecting the pollen into a brown bottle with silica gel, sealing the opening with a sealant, storing the brown bottle at normal temperature and in shade, exchanging the silica gel timely when color of the silica gel is changed, and sealing the bottle when the color of the silica gel is not changed and storing the bottle at a low temperature; and unfreezing, namely taking out the brown bottle and placing the brown bottle into a refrigerating chamber of a refrigerator for 20 to 24 hours, taking out the brown bottle and placing the brown bottle at a normal-temperature environment for 8 to 12 hours, opening the sealant and placing the brown bottle for 8 to 12 hours until the color of the silica gel is changed, or opening the opening if the color of the silica gel is not changed, adhering filter paper absorbed with water to the opening, and covering the opening by using a preservative film and sealing the opening by using a rubber band until the color of the silica gel is changed. By the method, life of the pinus massoniana pollen is prolonged; the problems of cross breeding (seed production) of pine tree seeds and flowering asynchronism of artificially auxiliary pollination in a seed orchard are solved; and utilization ratio of the pollen is increased.

The invention discloses a pinus massoniana embryogenic callus proliferation and maintenance culture method. The method includes: placing a filamentous embryogenic callus cell cluster obtained by induction of 3-4 weeks of a conventional somatic embryo and an embryogenic callus obtained by separation of a female gamete into a petri dish with a culture medium I; removing dead cells and unnecessary tissues in embryogenic calli in 4-6 weeks; transferring into a centrifugal tube, adding a culture medium II, mixing and well shaking; adding an embryogenic culture obtained after well shaking into a petri dish with a culture medium III; after 6-8 weeks, directly transferring an appropriate size of the embryogenic calli into a culture medium IV for normal subculture in a subculture period of 10-14d to finally obtain a pinus massoniana embryogenic cell line which is fast in growth, vigorous and available for long-term subculture. Evident browning and water soaking of the embryogenic calli in long-term subculture are avoided, the embryogenic calli grow fast, proliferation effects are stably high, the proliferation coefficient reaches 25 or above, and high economic benefits and social benefits are achieved.

The invention discloses a fermented kvass beverage and a preparation method thereof. The fermented kvass beverage takes the prepared beverage as 100 percent, and comprises the following ingredients in mass percent: 0.1-0.3 percent of sodium citrate, 1-3 percent of lactobacillus bulgaricus, 1-3 percent of thermophilic bacteria, 1-3 percent of beer yeast, 31-32 percent of coix seed bud juice, 59-60 percent of pinus massoniana pollen extracting solution and 2-4 percent of 55 type high fructose syrup. The preparation method comprises the following five steps of preparing the coix seed bud juice, preparing the pinus massoniana pollen extracting solution, acclimatizing strains, confirming a beverage formula, and preparing the fermented kvass beverage. The fermented kvass beverage has the characteristics of clear color, fragrant flavor and mellow taste.