Method for improving poria cocos mycelium protein content and liquid fermentation biomass

A technology of liquid fermentation and Poria cocos, applied in biochemical equipment and methods, microbes, treatment of microbes with electricity/wave energy, etc., can solve the problems of low biomass, low protein content of Poria cocos mycelium, slow growth rate, etc. Achieve high biomass, conducive to rapid growth, fast growth effect

Inactive Publication Date: 2014-03-12
HUAIHUA UNIV
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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for improving the protein content of Poria cocos mycelium and the biomass of liquid fermentation, so as to solve the problems of low protein content of Poria cocos mycelium, slow growth rate and low biomass

Method used

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Embodiment 1

[0019] 1. Strains: Poria cocos ( Wolfiporia cocos ) sclerotium, purchased from Fuling market in Jingzhou, Hunan, number: Xiangjing "28";

[0020] 2. The composition of the solid medium is: 1.0% to 4.0% of masson pine extract, 15% to 20% of potato boiled liquid, 0.8 to 1.2% of glucose, and 1.5% to 2.0% of agar;

[0021] 3. Liquid fermentation medium composition: 1.0% to 4.0% of masson pine extract, 0.8% to 3.5% of microcrystalline fiber, 1.0% to 3.5% of glucose, 0.01% to 0.05% of glycerin, 0.1% to 0.4% of ammonium sulfate, and 0.1% of magnesium sulfate %~0.3%, potassium hydroxide 0.02%~0.06%, phosphoric acid 0.02%~0.05%, pH 5.5;

[0022] 4. Strain mutagenesis and liquid fermentation culture: Wash the nearly mature Poria cocos sclerotia, draw a 3.0 cm long, 1.5 cm wide, and 1.0 cm deep opening on the surface of the crack, and cultivate it at a humidity of 90% and a temperature of 26°C for 15 days. The next day, after the mycelium growing from the wound was twisted to form ...

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Abstract

The invention relates to a bio-pharmaceuticals production technology, and in particular relates to a method for improving poria cocos mycelium protein content and liquid fermentation biomass. The method comprises the following steps: (1) preparing a poria cocos basidiospore suspension liquid; (2) mutating the poria cocos basidiospore through Co60-gamma radiation; (3) pairing basidiospore strains; (4) inoculating a pinus massoniana steep solid culture medium bevel, wherein the culture medium is composed of pinus massoniana extractive, potato extractive and glucose; and (5) inoculating a pinus massoniana extractive liquid fermentation culture medium, wherein the culture medium is composed of pinus massoniana extractive, microcrystal fiber, glucose, glycerinum, ammonium sulfate, magnesium sulfate, potassium hydroxide and phosphoric acid. The poria cocos strain with high mycelium protein content is firstly obtained through mutation, the adopted pinus massoniana steep solid culture medium bevel is in favor of the rapid growth of the poria cocos mycelium in comparison with the potato glucose culture medium, and the adopted pinus massoniana extractive liquid fermentation culture medium is in favor of obtaining high biomass.

Description

technical field [0001] The invention belongs to the field of production technology biopharmaceuticals, and mainly relates to a method for increasing the protein content of Poria cocos mycelium and the biomass of liquid fermentation. Background technique [0002] Poria cocos is a medicinal fungus, which is a traditional Chinese medicinal material and a food with a long history in my country. Sweet, light and flat, it has the effects of diuresis and dampness, invigorating the spleen and nourishing the middle, calming the mind and nerves, and has antibacterial, anti-aging, anti-tumor, anti-inflammatory, anti-viral and other effects. The protein content of Poria cocos mycelium affects its growth rate, and increasing the protein content of Poria cocos mycelium can promote the growth rate of mycelium, and the liquid submerged fermentation technology is an important way to obtain the main pharmacological active substances of Poria cocos, but the growth rate of Poria cocos mycelium ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N13/00
Inventor 不公告发明人
Owner HUAIHUA UNIV
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