40 results about "Basidiospore" patented technology

The invention discloses a site-specific insertional inactivation method mediated by agrobacterium tumefaciens and CRISPR/Cas9. Endogenous snRNA promoter of Ustilago scitaminea (u6 promoter of Ustilago scitaminea) is used to drive sgRNA expression cassette. CRISPR/Cas9 system integrates with Ti plasmid of agrobacterium tumefaciens to construct the Ustilago scitaminea site-specific insertional inactivation system mediated by agrobacterium tumefaciens with hygromycin as a selection marker. Specific sequence of the objective gene is cloned into sgRNA expression cassette for transformation of Ustilago scitaminea basidiospores, thus the mobile DNA fragment is exactly inserted into the objective gene sequence of Ustilago scitaminea, achieving the goal of damage to gene function. The invention provides an important tool for study of Ustilago scitaminea functional genomics. The system has the advantages of high efficiency and accuracy, and is convenient to use to conduct gene function researches in Ustilago scitaminea.

The invention relates to a bio-pharmaceuticals production technology, and in particular relates to a method for improving poria cocos mycelium protein content and liquid fermentation biomass. The method comprises the following steps: (1) preparing a poria cocos basidiospore suspension liquid; (2) mutating the poria cocos basidiospore through Co60-gamma radiation; (3) pairing basidiospore strains; (4) inoculating a pinus massoniana steep solid culture medium bevel, wherein the culture medium is composed of pinus massoniana extractive, potato extractive and glucose; and (5) inoculating a pinus massoniana extractive liquid fermentation culture medium, wherein the culture medium is composed of pinus massoniana extractive, microcrystal fiber, glucose, glycerinum, ammonium sulfate, magnesium sulfate, potassium hydroxide and phosphoric acid. The poria cocos strain with high mycelium protein content is firstly obtained through mutation, the adopted pinus massoniana steep solid culture medium bevel is in favor of the rapid growth of the poria cocos mycelium in comparison with the potato glucose culture medium, and the adopted pinus massoniana extractive liquid fermentation culture medium is in favor of obtaining high biomass.

The present invention discloses a kind of Ustilago esculenta strain UEZC1 capable of producing antifungal antibiotic and its use. The Ustilago esculenta strain UEZC1 features that the teleutospore is germinated on potato glucose liquid culture medium to form basidium promycelium with 1-4 basidiospore promycelia, and the basidiospore after falling off further grow to form fusiform short hypha for inserting basidiospore. The present invention can produce antifungal antibiotic, and may be used in developing and producing medical and agricultural antifungal antibiotic.

The invention discloses a method for producing polysaccharide by crossly culturing tremella fuciformis basidiospores by stages, comprising the following step that the fermenting and culturing process includes three stages which are respectively carried out on a rotary type swing bed and a reciprocated type swing bed at the same rotation speed for crossly culturing. By applying the method providedby the invention for culturing tremella fuciformis conidiums, tremella fuciformis polysaccharide can be produced in high efficiency, and the output of the polysaccharide can reach 5.80g/L. The method provided by the invention is a production method with favorable industrialized prospect, thereby laying the foundation for producing tremella fuciformis polysaccharide for the industrialized economy.

The invention discloses an application of farnesol in preventing and treating the smut disease of sugarcane and corn. According to the application of farnesol in preventing and treating smut disease,the life history of sugarcane smut can be affected. Farnesol has better inhibiting effect on the germination of teliospore, the proliferation and growth of basidiospore and the sexual cooperation. Theformation of the binuclear mycelium can be effectively blocked, the sugarcane can not be normally infected with sugarcane smut, and the occurrence of the smut disease of the sugarcane can be effectively inhibited; the proliferation and growth of corn smut can be affected and the occurrence of corn smut can be inhibited.

The invention provides a medium formula for promoting directional formation of inonotus obliquus fruiting bodies and a method and belongs to a medium formula for directionally obtaining the inonotus obliquus fruiting bodies. The medium is prepared from raw materials in percentage by mass as follows: 55%-65% of weed tree sawdust, 20% of rice husks, 2% of glucose, 0.5% of magnesium sulfate, 1.5% of gypsum, 5% of bran and 6%-16% of Fomitopsis pinicola fruiting body scraps, and a material-water ratio is 1:1.2-1.3. According to the medium formula, an inonotus obliquus carbon source and a small amount of nitrogen, inorganic matter and the like can be provided through mixing of the weed tree sawdust and the rice husks, the rice husks have a breathable function, the glucose is directly-usable monosaccharide and beneficial to initial colonization of inonotus obliquus mycelia, the bran provides a nitrogen source, and the Fomitopsis pinicola fruiting body scraps have a function of forming fruiting bodies through directional induction. Cultivation management of inonotus obliquus is a mature technology; with the adoption of the medium formula, the fruiting bodies can be directly formed through direct solid fermentation, the inonotus obliquus fruiting bodies capable of producing basidiospores can be obtained through conventional cultivation, and the obtained fruiting bodies are used for artificial breeding and development of food and drugs.

The invention relates to the technical field of edible mushroom cultivation, in particular to a technology for planting straw mushroom by taking sisal hemp slag as a raw material. The technology comprises the steps of preparing compost by taking the sisal hemp slag as the raw material; carrying out inoculation on the compost by stacking and fermenting the compost; placing the inoculated compost in a sterilized mushroom room for cultivating, and controlling different growth temperatures, different pH (Potential of Hydrogen) and different water contents in a basidiospore budding stage, a mycelial growth stage and a sporocarp development stage; and picking the straw mushroom, sending the straw mushroom to a processing workshop to be classified and packaged, and putting the straw mushroom in a refrigerating room for preserving. According to the technology disclosed by the invention, the cost of the raw material is lowered, the food safety is ensured, and the growth cycle of the straw mushroom is shortened.

The invention discloses a lucid ganoderma basidiospore germination culture medium, and a method using the lucid ganoderma basidiospore germination culture medium to germinate lucid ganoderma basidiospore. The lucid ganoderma basidiospore germination culture medium is prepared from a lucid ganoderma decocting liquid, a PDA (potato dextrose agar) powder and water. The lucid ganoderma basidiospore germination method has the advantages that the germination rate of lucid ganoderma basidiospore is 10% or above, and is even up to 20%, so as to obtain lucid ganoderma spore monokaryontic strains; the problem of lower germination rate of lucid ganoderma basidiospore under the laboratory condition is solved, so as to lay a foundation for the monospore crossbreeding of lucid ganoderma.

The invention provides an enteral full-nutrition preparation suitable for a tumor patient in a chemoradiotherapeutic period and a preparation method thereof. The enteral full-nutrition preparation is prepared by mixing and drying silkie enzymatic hydrolysate, isolated soybean protein meal, endothelium corneum gigeriae galli powder, virgin olive oil, perilla oil, germinated brown rice flour, purple potato fermenting liquid, concentrated Chinese jujube juice, selenium-enriched malt powder, barleygreen powder, spirulina powder, tremella fuciformis basidiospore powder, sea cucumber powder, fructo-oligose powder, compound mineral and compound vitamin. The enteral full-nutrition preparation achieves a synergetic effect among the components, reasonably supplements full nutrition and sufficient energy, reduces bone marrow suppression, digestive symptoms, mucosa damage and other adverse reactions caused by chemoradiotherapy, improves resistance to tumor therapy, the therapeutic effect and the body immunity, and ultimately prolongs the survival time of the tumor patient, so that the enteral full-nutrition preparation has a very important social value.

The invention relates to a method for purifying a mononuclear bacterial strain of a needle mushroom. The method for purifying the mononuclear bacterial strain of the needle mushroom is characterized by including the steps that basidiospores collected from the sporocarp of the needle mushroom are grafted to a flat plate with PDA culture media to be cultivated so as to form a circular bacterial colony, the hypha of 1-2mm with PDA culture media in a connecting mode can be picked on the edge of the bacterial colony to be used as a grafting block, the hypha is grafted to a novel flat plate with PDA culture media, cultivation is conducted until a radial pattern hypha grows out around the grafting block, a single hypha is picked again to put into novel PDA culture media to be cultivated, cultivation is carried out continuously for 6-7 days so that a purified bacterial colony can be obtained, and the hypha is selected at will to be cultivated again to obtain the purified bacterial strain of the needle mushroom. The formula of the PDA culture media is that 20g of peeled potatoes are used for conducting juice boiling, 20g of glucose, 20g of agar and 1,000ml of distilled water with the twain (80) being 0.1ml and the pH value being 6.5-6.8 are poured onto the flat plate, and 18-20m of the mixing liquid exists on each plat plate. By means of the method for purifying the mononuclear bacterial strain of the needle mushroom, the highly-purified mononuclear bacterial strain can be obtained.

The invention belongs to the technical field of edible fungus breeding, and relates to a gjaponicum basidiospore germination medium and a germination method of gjaponicum basidiospore. Every 1,000 mLof the germination medium is prepared from 160-240 g of potatoes, 10-20 g of glucose, 3-8 g of saccharose, 0.8-1.5 g of MgSO4, 0.3-0.8 g of KH2PO4, 1.5-2 g of agar powder, 150-200 mL of gjaponicum cooked liquid, 40-80 microliters of glacial acetic acid and the balance distilled water. According to the gjaponicum basidiospore germination medium, the glacial acetic acid with the specific content isadded, the glacial acetic acid cooperates with the potatoes, the glucose, the saccharose, MgSO4, KH2PO4, the agar powder and the gjaponicum cooked liquid, and the germination rate of the gjaponicum basidiospore is increased to 6.50%. The method for germinating the gjaponicum basidiospore with the gjaponicum basidiospore germination medium is simple, easy to operate and easy to apply and popularizeon a large scale.

The invention discloses an avermectin derivative, which is extracted and separated from Anthogorgia caerulea, and is named as 5-O-dimethyl-25-bis(1-methylpropyl)-25-(1-methylbutyl)avermectin Ala. Tests indicate that the EC50 value of the avermectin derivative Blc on ustilago scitaminea MAT-1 and MAT-2 matching basidiospore proliferation is far less than a positive control azoxystrobin suspending agent, and has good avermectin derivative inhibition activity. The compound Blc is a marine natural active component, also is easily soluble in methanol and ethanol, and has good alcohol affinity, thus being applicable individually or in combination to preparation of efficient biopesticides for control of sugarcane ustilago. The invention provides a new use for development of avermectin derivatives, and provides a new source for preparation of novel pesticides inhibiting ustilago scitaminea.

The invention discloses a stationary liquid for microscopically observing hypsizigus marmoreus basidiospores, a preparation method, a fixing method and application. The stationary liquid provided by the invention is prepared by mixing molten gelatin and a glycerol hydration agent. The stationary liquid obtained by the method is good in light transmission and safe in phase change temperature, and the method is simple, convenient and rapid, can be used for batch production, and can also be used for temporary preparation in case of urgent need. The fixing liquid is used for fixing the hypsizigusmarmoreus basidiospores, other treatment effects such as dyeing of the hypsizigus marmoreus basidiospores are not affected, the exposure time can be prolonged, and the quality of obtained hypsizigus marmoreus basidiospore image data is improved.

A novel hybrid fungus culture, designated J9277, of the mushroom species Agaricus bisporus produces crops of mushrooms having white, rounded, thick-fleshed caps and proportionally long stems in a relatively short interval of time. Diverse additional strains can be developed from J9277 by various means including somatic and tissue culture selection, basidiospore selection, and hybridization to other strains of Agaricus bisporus, and the resulting derivative strains can be screened for desirable commercial characteristics.

The invention discloses a method for collecting clean basidiospores of toadstool fungi. The method comprises: wiping the surface of a fruit body by dipping 70% ethanol with a cotton ball, cutting offthe stipe from the joint between the stipe and the cap, and placing the cap upside down on a super clean bench; folding the sulfuric acid paper twice, then cutting off the right-angle part, spreadingand placing the sulfuric acid paper into the bottom of a petri dish, taking two autoclavable rubber bands to sleeve the petri dish, placing the cap on the rubber bands, with gills or pores facing down, and covering the petri dish to stand for 4 to 24 hours; removing the cap of the fruit body, removing the rubber bands, folding the sulfuric acid paper inward twice, so that the spore-bearing side islocated on the inner side, spreading the sulfuric acid paper into a funnel shape, placing the same above the mouth of a centrifuge tube, and washing the inside of the sulfuric acid paper uniformly with sterile water to collect the basidiospores. The method of the invention can obtain clean basidiospores, has a high spore recovery rate and is simple to operate.

The invention discloses an efficient domestication cultivation method for a Phlebopus portentosus strain, belonging to the technical field of microorganisms. The efficient domestication cultivation method comprises the following steps: (1) collecting Phlebopus portentosus sporocarps; (2) collecting basidiospores; (3) carrying out spore suspension liquid pretreatment; (4) germinating the basidiospores, so as to obtain single spore strains; (5) culturing the single spore strains, so as to obtain to-be-screened fusion strains; (6) culturing the fusion strains, and screening dominant strains; and(7) culturing the dominant strains, and carrying out cryopreservation. By carrying out domestication and screening, the saprophytic ability of the Phlebopus portentosus is improved, so that the artificial cultivation yield is effectively increased, and the artificial cultivation stability is effectively improved. The fruiting rate of the strain domesticated by virtue of the method reaches over 85%, the maturing rate of the sporocarps reaches over 80%, and the form is complete; the common biological efficiency within about 20% at present can be increased to be over 38% and can be maximally increased to be 47.5%, and the appearance and yield trait are stable.

The invention discloses a method for efficiently acquiring basidiospore homocaryon of agaricus bisporus. The method comprises: step one, carrying out agaricus bisporus planting; to be specific, selecting agaricus bisporus types and planting and cultivating samples in agaricus bisporus planting equipment; step two, carrying out sampling; to be specific, at an agaricus bisporus picking stage, carrying out agaricus bisporus sampling at five developmental stages including a veil non-appearing stage, a veil initial appearing stage, a veil inward recession stage, a veil flattening stage, and a veilfracture stage based on a time sequence; step three, carrying out analysis; to be specific, selecting mediotrastum of agaricus bisporus at different development stages, carrying out statistics of thenumber of basidia with sporophore growing at mediotrastum at different development stages as well as the proportions of different basidia; step four, determining an optimal obtaining period; to be specific, determining at least one development stage as an optimal obtaining period based on the result from the step three; and step five, picking basidiospore homocaryon in batches. According to the invention, the basidiospore homocaryon of agaricus bisporus is obtained at the veil initial appearing stage of the agaricus bisporus, so that the obtaining efficiency is improved substantially.

The invention relates to the technical field of edible fungus cultivation, and particularly discloses a black fungus strain JH40 and application thereof. The strain preservation number of the black fungus strain JH40 is CCTCC No: M20191058. The invention also provides protoplasts, basidiospores, mycelia and sporophores generated by the black fungus strain JH40. The black fungus strain JH40 provided by the invention can be applied to black fungus cultivation and health care product development. Compared with a traditional black fungus strain, the black fungus strain JH40 provided by the invention has stronger environmental adaptability, resists high temperature, can normally grow in an environment of 30 DEG C or above, and has the excellent qualities of a short growth period, high biological efficiency, good genetic stability, thick auricle, high soaking rate and the like.

The invention discloses an edible fungus spore print collecting method, which comprises the following steps: selecting seed mushroom, cutting off mushroom stems, burying the upper surface of the seed mushroom fruiting body pileus into a culture medium, after the culture medium is solidified, fixing the fruiting body and enabling the gill or the mushroom tube surface to face downwards, buckling the fruiting body on the bottom of another culture dish with aluminum foil paper, sealing the culture dish with a sealing film, performing ejection at a proper culture temperature, performing gradient dilution with sterile water to 5-10 basidiospore/100 microliters, coating on a PDA flat plate, performing constant-temperature culture at a proper temperature, and when small colonies grow on the flat plate, timely transferring the small colonies to a new PDA culture medium for culture. According to the invention, the method is simple to operate; fruiting body is fixed by using a solidified culture medium, so that the spore print collection method is greatly simplified, the trouble of infectious microbe pollution is eliminated, a plurality of spore prints can be stacked, and the collection of a plurality of spore prints can be conveniently performed at the same time; and the collected spore prints can be coated by aluminum-foil paper for preservation, so that the later use is facilitated.