The invention discloses an
edible fungus spore print collecting method, which comprises the following steps: selecting seed
mushroom,
cutting off
mushroom stems, burying the upper surface of the seed
mushroom fruiting body
pileus into a culture medium, after the culture medium is solidified, fixing the fruiting body and enabling the
gill or the mushroom tube surface to face downwards, buckling the fruiting body on the bottom of another culture dish with
aluminum foil paper, sealing the culture dish with a sealing film, performing ejection at a proper culture temperature, performing gradient
dilution with
sterile water to 5-10
basidiospore / 100 microliters,
coating on a PDA flat plate, performing constant-temperature culture at a proper temperature, and when small colonies grow on the flat plate, timely transferring the small colonies to a new PDA culture medium for culture. According to the invention, the method is simple to operate; fruiting body is fixed by using a solidified culture medium, so that the
spore print collection method is greatly simplified, the trouble of infectious microbe
pollution is eliminated, a plurality of
spore prints can be stacked, and the collection of a plurality of spore prints can be conveniently performed at the same time; and the collected spore prints can be coated by aluminum-foil paper for preservation, so that the later use is facilitated.