Edible fungus spore print collecting method

Abstract

The invention discloses an edible fungus spore print collecting method, which comprises the following steps: selecting seed mushroom, cutting off mushroom stems, burying the upper surface of the seed mushroom fruiting body pileus into a culture medium, after the culture medium is solidified, fixing the fruiting body and enabling the gill or the mushroom tube surface to face downwards, buckling the fruiting body on the bottom of another culture dish with aluminum foil paper, sealing the culture dish with a sealing film, performing ejection at a proper culture temperature, performing gradient dilution with sterile water to 5-10 basidiospore / 100 microliters, coating on a PDA flat plate, performing constant-temperature culture at a proper temperature, and when small colonies grow on the flat plate, timely transferring the small colonies to a new PDA culture medium for culture. According to the invention, the method is simple to operate; fruiting body is fixed by using a solidified culture medium, so that the spore print collection method is greatly simplified, the trouble of infectious microbe pollution is eliminated, a plurality of spore prints can be stacked, and the collection of a plurality of spore prints can be conveniently performed at the same time; and the collected spore prints can be coated by aluminum-foil paper for preservation, so that the later use is facilitated.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for collecting edible fungus spore prints. Background technique [0002] The existing spore print collection methods mainly include hook suspension method, glass bell jar method, direct ejection onto sterile paper, and smearing method on fruiting body folds, etc. Among them, the spore prints collected by the first two methods can well avoid bacterial contamination However, the operation is cumbersome, and the equipment is complicated, which is not conducive to collecting multiple spore prints at the same time; although the latter two methods are simple to operate, they are easy to cause bacterial contamination, and they are directly ejected onto sterile paper to obtain spore prints in the later spore prints. Cloth is easily polluted by miscellaneous bacteria during cultivation, which is unfavorable for picking and cultivating mycelia. Similarly, the me...

AI Technical Summary

Problems solved by technology

[0003] Aiming at the deficiencies in the background technology, the present invention provides a method for collecting spore prints of edible fungi, which overcomes the difficulty of the existing spore print collection technology in both simple operation and free from contamination by miscellaneous bacteria, simplifies the spore print collection technology, and facilitates the collection of edible fungi. Breeding work carried out

Images