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A technology for edible fungi and spores, applied in the field of microorganisms, can solve problems such as difficulty in taking into account the simplicity of operation, and achieve the effects of reducing experimental costs, occupying small space, and simple operation.
Pending Publication Date: 2022-02-08
河北华绿之珍生物技术有限公司
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Problems solved by technology
[0003] Aiming at the deficiencies in the background technology, the present invention provides a method for collecting spore prints of edible fungi, which overcomes the difficulty of the existing spore print collectio
Method used
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Embodiment 1
[0027] Present embodiment selects Flammulina velutipes for use, carries out spore print collection to it, and concrete steps are as follows:
[0028] (1) The selection principle of Flammulina velutipes mushrooms: choose 80-90% mature mushrooms with strong individuals, good flower shape, no pests and diseases, uniform fruiting, high and stable yield, and strong adaptability.
[0029] (2) Collect the selected seed mushrooms and open the umbrella, select the mushroom caps with round mushroom caps and moderate sizes, disinfect them with 75% alcohol, put them into the ultra-clean workbench, and place the collected mushroom caps on the ultra-clean operating table In a sterilized petri dish, cut off the stipe, divide into several pieces of appropriate size, and set aside.
[0030] (3) Take the sterilized agar aqueous solution, pour it into a sterilized petri dish and cover it with a small amount when it is about to solidify. Facing downward, buckle it on the bottom of the petri dish...
Embodiment 2
[0036] The present embodiment selects Grifola frondosa for use, and carries out spore print collection to it, and concrete steps are as follows:
[0037] (1) The selection principle of Grifola frondosa mushrooms: choose seven or eight mature mushrooms with robust individuals, good flower shape, no pests and diseases, uniform fruiting, high and stable yield, and strong adaptability.
[0038] (2) Collect the selected seed mushrooms, select the caps with good ears and moderate size, disinfect them with 75% alcohol, put them into the ultra-clean workbench, and place the collected caps on the ultra-clean operating table for sterilization culture In a dish, cut off the stipe, divide it into several pieces of appropriate size, and set aside.
[0039] (3) Take sterilized agar aqueous solution, pour it into a sterilized petri dish when it is about to solidify and cover it with a little cover, take the cap of the fruiting body of edible fungus, bury the upper surface in the culture medi...
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Abstract
The invention discloses an edible fungus spore print collecting method, which comprises the following steps: selecting seed mushroom, cutting off mushroom stems, burying the upper surface of the seed mushroom fruiting body pileus into a culture medium, after the culture medium is solidified, fixing the fruiting body and enabling the gill or the mushroom tube surface to face downwards, buckling the fruiting body on the bottom of another culture dish with aluminum foil paper, sealing the culture dish with a sealing film, performing ejection at a proper culture temperature, performing gradient dilution with sterile water to 5-10 basidiospore/100 microliters, coating on a PDA flat plate, performing constant-temperature culture at a proper temperature, and when small colonies grow on the flat plate, timely transferring the small colonies to a new PDA culture medium for culture. According to the invention, the method is simple to operate; fruiting body is fixed by using a solidified culture medium, so that the spore print collection method is greatly simplified, the trouble of infectious microbe pollution is eliminated, a plurality of spore prints can be stacked, and the collection of a plurality of spore prints can be conveniently performed at the same time; and the collected spore prints can be coated by aluminum-foil paper for preservation, so that the later use is facilitated.
Description
technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for collecting edible fungus spore prints. Background technique [0002] The existing spore print collection methods mainly include hook suspension method, glass bell jar method, direct ejection onto sterile paper, and smearing method on fruiting body folds, etc. Among them, the spore prints collected by the first two methods can well avoid bacterial contamination However, the operation is cumbersome, and the equipment is complicated, which is not conducive to collecting multiple spore prints at the same time; although the latter two methods are simple to operate, they are easy to cause bacterial contamination, and they are directly ejected onto sterile paper to obtain spore prints in the later spore prints. Cloth is easily polluted by miscellaneous bacteria during cultivation, which is unfavorable for picking and cultivating mycelia. Similarly, the me...
Claims
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Application Information
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