Efficient domestication cultivation method for Phlebopus portentosus strain

Abstract

The invention discloses an efficient domestication cultivation method for a Phlebopus portentosus strain, belonging to the technical field of microorganisms. The efficient domestication cultivation method comprises the following steps: (1) collecting Phlebopus portentosus sporocarps; (2) collecting basidiospores; (3) carrying out spore suspension liquid pretreatment; (4) germinating the basidiospores, so as to obtain single spore strains; (5) culturing the single spore strains, so as to obtain to-be-screened fusion strains; (6) culturing the fusion strains, and screening dominant strains; and(7) culturing the dominant strains, and carrying out cryopreservation. By carrying out domestication and screening, the saprophytic ability of the Phlebopus portentosus is improved, so that the artificial cultivation yield is effectively increased, and the artificial cultivation stability is effectively improved. The fruiting rate of the strain domesticated by virtue of the method reaches over 85%, the maturing rate of the sporocarps reaches over 80%, and the form is complete; the common biological efficiency within about 20% at present can be increased to be over 38% and can be maximally increased to be 47.5%, and the appearance and yield trait are stable.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a high-efficiency domestication and cultivation method for Dictyostelium boletus strains. Background technique [0002] Phlebopus portentosus, also known as Phlebopus portentosus, or strange-shaped brown round hole boletus, commonly known as black boletus, belongs to Boletes Boletales, Boletaceae Boletinellaceae, Phlebopus genus; mainly distributed in pantropical regions, including Yunnan, Sichuan, Guangxi, Hainan and other places in my country, and Thailand, Sri Lanka, Vietnam, Indonesia, Australia, New Zealand, Africa, Mexico and other places abroad. Commonly known as "black boletus", the dark brown reticulum boletus is one of the most popular wild edible fungi in southern Yunnan and northern Thailand because of its delicious taste and rich nutrition. [0003] Dictyostelium boletus is a high-protein, low-fat food, rich in various mineral elements, has strong reducing abi...

AI Technical Summary

Problems solved by technology

Although wild Dictyostelium boletus can produce fruiting bodies without the participation of plants, the occurrence efficiency is very low

[0008] However, the current artificial cultivation methods for mushroom houses have the problem of low unit yield, and the general biological efficiency is only about 20%. For example, Chinese patent application No. 200910094157.4 discloses that the mycelium of the solid strains cultivated for 50 days is overgrown with 700ml culture Base, the mycelium and fruiting body (fresh mushroom) value gap reported by the patent is huge, and the mycelium is not commercial

Moreover, the problem of unstable fruiting requires the domestication of Dictyostelium bursa.

[0009] Chinese Patent Application No. 201410413765.8 discloses a method of domestication. In this method, the fruiting body of Boletus nigricans with excellent properties is used as a domesticated strain, and the meat tissue of the domesticated bacterial strain is inoculated on a nutrient-poor substrate to obtain Boletus mycelium. The hyphae of liver fungus are repeatedly induced and domesticated in the matrix rich in lignin, C, and N nutrient-poor, which can induce the ability of black boletus to decompose and utilize lignin and cellulose, and enhance the decomposition of black boletus Utilize the ability of lignin and cellulose to improve the ability to resist impurities, thereby improving the fruiting rate, fruiting uniformity and maturity rate, and improving the biological efficiency of black boletus cultivation, and the obtained black boletus has excellent properties. The patent is also a strain domesticated based on the separation of fruiting body tissues. Its genetic traits are relatively fixed, but its biological efficiency is only 22% at its highest.

Another example is that Chinese Patent Application No. 200910094157.4 discloses a method for cultivating Dictyostelium phalaensis boletus, which realizes rapid cultivation from the mother species, liquid strains to solid strains; the patent report is basically based on the separation and domestication of fruiting body tissues. The strains from which the genetic traits are relatively fixed

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