Efficient domestication cultivation method for Phlebopus portentosus strain

A dark brown reticulum and cultivation method technology, applied in the field of microorganisms, can solve the problems of low unit yield, low occurrence efficiency, fixed genetic traits, etc., and achieve the effects of increased yield and stability, stable yield, and complete form
CN109673392APending Publication Date: 2019-04-26秦小波
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
秦小波
Publication Date
2019-04-26

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Abstract

The invention discloses an efficient domestication cultivation method for a Phlebopus portentosus strain, belonging to the technical field of microorganisms. The efficient domestication cultivation method comprises the following steps: (1) collecting Phlebopus portentosus sporocarps; (2) collecting basidiospores; (3) carrying out spore suspension liquid pretreatment; (4) germinating the basidiospores, so as to obtain single spore strains; (5) culturing the single spore strains, so as to obtain to-be-screened fusion strains; (6) culturing the fusion strains, and screening dominant strains; and(7) culturing the dominant strains, and carrying out cryopreservation. By carrying out domestication and screening, the saprophytic ability of the Phlebopus portentosus is improved, so that the artificial cultivation yield is effectively increased, and the artificial cultivation stability is effectively improved. The fruiting rate of the strain domesticated by virtue of the method reaches over 85%, the maturing rate of the sporocarps reaches over 80%, and the form is complete; the common biological efficiency within about 20% at present can be increased to be over 38% and can be maximally increased to be 47.5%, and the appearance and yield trait are stable.
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Description

technical field

[0001] The invention relates to the technical field of microorganisms, in particular to a high-efficiency domestication and cultivation method for Dictyostelium boletus strains. Background technique

[0002] Phlebopus portentosus, also known as Phlebopus portentosus, or strange-shaped brown round hole boletus, commonly known as black boletus, belongs to Boletes Boletales, Boletaceae Boletinellaceae, Phlebopus genus; mainly distributed in pantropical regions, including Yunnan, Sichuan, Guangxi, Hainan and other places in my country, and Thailand, Sri Lanka, Vietnam, Indonesia, Australia, New Zealand, Africa, Mexico and other places abroad. Commonly known as "black boletus", the dark brown reticulum boletus is one of the most popular wild edible fungi in southern Yunnan and northern Thailand because of its delicious taste and rich nutrition.

[0003] Dictyostelium boletus is a high-protein, low-fat food, rich in various mineral elements, has strong reducing abi...

Claims

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