3 results about "Boletales" patented technology

The present application belongs to the technical field of application of fungal polysaccharides, and particularly relates to a Boletus edulis fruiting body polysaccharide as well as a preparation method and application thereof. Polysaccharides in Boletus edulis fruiting bodies are extracted, and the chemical structure of BDP-F is characterized by ultraviolet-visible light, Fourier transform infrared spectroscopy, one-dimensional nuclear magnetic resonance and two-dimensional nuclear magnetic resonance. The research results show that BDP-F is mainly composed of mannose, glucose, galactose and fucose. Methylation and nuclear magnetic resonance analysis show that the glycosidic bond of BDP-F is (1→6)-alpha-d-galactose, the main chain is composed of ->6)-alpha-d-galp-(1→ residues, and the O-2 position is branched by alpha-L-Fucp-(1→ and alpha-D-Manp-(1→ residues. Through DPPH free radical, hydroxyl radical and superoxide anion radical scavenging experiments, it is shown that BDP-F has the ability to serve as a natural antioxidant, and BDP-F can also reduce alcohol-induced GES-1 cell damage through antioxidant and anti-inflammatory effects within the experimental concentration range.

This invention discloses a strain of *Boletus urinaria* PP013 and its applications, with the preservation number CGMCC No. 40661. This invention also provides a sustainable, cyclical understory cultivation method, including liquid spawn culture, spawn culture, soil covering for fruiting, and understory inoculation. This method selects shallow-rooted tree species such as *Delonix regia* and *Gardenia jasminoides*, and lays a layer of dry weeds consisting of sugarcane bagasse, miscanthus, and *Digitaria sanguinalis* under the trees. Artificially cultivated fruiting bodies about to open their gills are placed under the trees as a live spore source for spore production. After spore production, the understory is covered with dry weeds and watered. The following year, after fruiting, some fruiting bodies are retained for natural spore production, achieving a single inoculation and multi-year cyclical fruiting. This invention creatively utilizes fruiting body spores for natural inoculation, replacing the traditional direct mycelial inoculation method. It boasts a high success rate of establishment, low long-term production costs, and represents a highly efficient forest-fungus integrated management model.

This invention belongs to the field of fungal polysaccharide application technology, specifically relating to a method for preparing and applying polysaccharide from *Boletus globosum*. Crude polysaccharide was extracted from the fruiting body of *Boletus globosum* using hot water extraction, and purified using DEAE-52 cellulose column chromatography. Subsequently, by establishing an alcohol-induced liver injury model, it was found that NOP-1 significantly reduced ALT and AST levels, while simultaneously decreasing MDA content and increasing SOD activity and GSH levels, indicating that NOP-1 can inhibit lipid peroxidation, enhance the antioxidant defense capacity of hepatocytes, and maintain hepatocyte membrane integrity. A HepG2 cell IR model was constructed using a high-glucose, high-insulin combined induction method, determining the optimal dosage of NOP-1. ‑6 Treatment of HepG2 cells with mol / L insulin for 24 h successfully induced insulin resistance. Glucose consumption experiments in IR HepG2 cells demonstrated that NOP-1 significantly enhanced the glucose consumption capacity of insulin-resistant cells, exhibiting significant in vitro hypoglycemic activity.