9 results about "Boletales" patented technology

The present application belongs to the technical field of application of fungal polysaccharides, and particularly relates to a Boletus edulis fruiting body polysaccharide as well as a preparation method and application thereof. Polysaccharides in Boletus edulis fruiting bodies are extracted, and the chemical structure of BDP-F is characterized by ultraviolet-visible light, Fourier transform infrared spectroscopy, one-dimensional nuclear magnetic resonance and two-dimensional nuclear magnetic resonance. The research results show that BDP-F is mainly composed of mannose, glucose, galactose and fucose. Methylation and nuclear magnetic resonance analysis show that the glycosidic bond of BDP-F is (1→6)-alpha-d-galactose, the main chain is composed of ->6)-alpha-d-galp-(1→ residues, and the O-2 position is branched by alpha-L-Fucp-(1→ and alpha-D-Manp-(1→ residues. Through DPPH free radical, hydroxyl radical and superoxide anion radical scavenging experiments, it is shown that BDP-F has the ability to serve as a natural antioxidant, and BDP-F can also reduce alcohol-induced GES-1 cell damage through antioxidant and anti-inflammatory effects within the experimental concentration range.

The invention discloses a method for breeding by culturing sporocarp under pure culture conditions of boletus sinensis, and belongs to the technical field of edible mushroom breeding and cultivation. The method comprises the following steps: activating the boletus sinensis strain JSJ-Bx1, inoculating the boletus sinensis strain JSJ-Bx1 into a culture medium for dark culture, then performing low-temperature induction culture on an activated flat plate which is nearly overgrown with hyphae, changing the hyphae from white to golden yellow and generating hypha kinking, then transferring the hypha flat plate into a fruiting room for culture, and after obvious primordium differentiation is seen, inoculating the hyphae into a culture medium for culture, thereby obtaining the boletus sinensis strain JSJ-Bx1. Continuously performing induced culture to develop into mature sporocarp; sporocarp basidiospores are collected, diluted and coated in a B1 culture medium for germination culture, and monospora hyphae and polyspora hyphae are obtained and serve as stock seeds for breeding the boletus sinensis. According to the method, the mature sporocarp of the boletus sinensis can be directly and rapidly cultured in the agar culture medium, the harvested basidiospore can normally germinate and grow, and the whole life history of the boletus sinensis can be completed under the pure culture condition.

The invention relates to the technical field of fungus cultivation, in particular to a boletus edulis planting method with a high nutrition conversion rate. Comprising the following steps: fermenting bagasse to obtain fermented bagasse; uniformly mixing the crushed fermented bagasse and eucalyptus wood chips with soybean powder, oat powder and lime powder, and adding water until the water content reaches 50-55% to obtain a culture medium; the culture medium is bagged, sterilized and cooled, and the treated culture medium is obtained; inoculating a liquid strain of the boletus edulis into the treated culture medium, and culturing in a dark place to obtain hyphae of the boletus edulis; the method comprises the following steps: covering the surfaces of boletus edulis hyphae with rice field soil, controlling the temperature, humidity and illumination culture, then adjusting the temperature and humidity, and continuously culturing for 5-7 days to obtain the boletus edulis with high nutrition conversion rate. According to the planting method, the nutritional ingredients in the boletus edulis are effectively increased, the production cost of the boletus edulis is reduced, the strain culture time is shortened, the hybrid resistance is high, and the nutrition conversion rate is high.

The invention discloses a boletus aereus solid strain culture method, and relates to the technical field of microbial culture, the method comprises the following steps: preparing a special culture medium taking hard broadleaf sawdust with specific particle size and corncob as main materials, wheat bran and soybean meal as auxiliary materials, and mulberry twig fermentation dry powder as a biological activity induction component; putting the special solid culture medium into a culture bottle, sealing, sterilizing, and cooling for later use; inoculating the boletus aereus mother strain into the sterilized special solid culture medium; a micro-positive pressure intermittent ventilation culture process which is closed in the whole process and does not need punching is adopted for the culture bottle after culture and inoculation. The invention aims to solve the problems of high pollution risk, slow hypha growth and high planting failure rate caused by manual punching due to compact structure and poor air permeability of the existing solid culture gene, realizes the cooperation of high-porosity oxygen supply, precise nutrition supply and sterile environment guarantee, and remarkably improves the hypha activity and culture success rate. The method is suitable for efficient industrial production of boletus aereus solid strains.

This invention provides a composite polysaccharide-selenium nanoparticle complex, its preparation method, and its applications. The complex comprises polysaccharide-modified selenium nanoparticles, obtained by a redox reaction of a polysaccharide composition with a selenium solution. The polysaccharide composition includes *Boletus edulis* polysaccharide, *Portulaca oleracea* polysaccharide, and *Astragalus membranaceus* polysaccharide, with a mass ratio of 0.5–3:0.5–3:0.5–3. The composite polysaccharide-selenium nanoparticle complex of this invention, through the synergistic modification of selenium nanoparticles by multiple polysaccharides, effectively prevents the aggregation and oxidation of selenium nanoparticles, significantly improves the dispersibility and stability of selenium nanoparticles, enhances their bioavailability and targeting, strengthens their antioxidant activity, reduces their cytotoxicity, and achieves multifunctionality, expanding the application potential of selenium nanoparticles in medicine, food, cosmetics, and other fields.

The application discloses a method for cultivating fruit bodies of Chinese saprobic boletus under pure culture conditions, and belongs to the technical field of edible fungus breeding and cultivation. The method comprises the following steps: inoculating a Chinese saprobic boletus strain JSJ-Bx1 after activation into a culture medium for dark culture, then performing low-temperature induction culture on the plate with activated mycelium close to full growth, changing the mycelium from white to golden yellow, and causing mycelium kinking, then transferring the mycelium plate to a fruiting room for culture, continuing to induce culture to develop into mature fruit bodies after obvious primordium differentiation is observed, and collecting fruit body basidiospores, diluting and coating the basidiospores on a B1 culture medium for germination culture, obtaining pseudomonosporic mycelium and polysporic mycelium, and using the pseudomonosporic mycelium and polysporic mycelium as the original strain for Chinese saprobic boletus breeding. The application can directly and rapidly culture Chinese saprobic boletus mature fruit bodies on an agar culture medium, the harvested basidiospores can normally germinate and grow, and the Chinese saprobic boletus can complete the whole life history under pure culture conditions.

This invention discloses a strain of *Boletus urinaria* PP013 and its applications, with the preservation number CGMCC No. 40661. This invention also provides a sustainable, cyclical understory cultivation method, including liquid spawn culture, spawn culture, soil covering for fruiting, and understory inoculation. This method selects shallow-rooted tree species such as *Delonix regia* and *Gardenia jasminoides*, and lays a layer of dry weeds consisting of sugarcane bagasse, miscanthus, and *Digitaria sanguinalis* under the trees. Artificially cultivated fruiting bodies about to open their gills are placed under the trees as a live spore source for spore production. After spore production, the understory is covered with dry weeds and watered. The following year, after fruiting, some fruiting bodies are retained for natural spore production, achieving a single inoculation and multi-year cyclical fruiting. This invention creatively utilizes fruiting body spores for natural inoculation, replacing the traditional direct mycelial inoculation method. It boasts a high success rate of establishment, low long-term production costs, and represents a highly efficient forest-fungus integrated management model.

This invention belongs to the field of fungal polysaccharide application technology, specifically relating to a method for preparing and applying polysaccharide from *Boletus globosum*. Crude polysaccharide was extracted from the fruiting body of *Boletus globosum* using hot water extraction, and purified using DEAE-52 cellulose column chromatography. Subsequently, by establishing an alcohol-induced liver injury model, it was found that NOP-1 significantly reduced ALT and AST levels, while simultaneously decreasing MDA content and increasing SOD activity and GSH levels, indicating that NOP-1 can inhibit lipid peroxidation, enhance the antioxidant defense capacity of hepatocytes, and maintain hepatocyte membrane integrity. A HepG2 cell IR model was constructed using a high-glucose, high-insulin combined induction method, determining the optimal dosage of NOP-1. ‑6 Treatment of HepG2 cells with mol / L insulin for 24 h successfully induced insulin resistance. Glucose consumption experiments in IR HepG2 cells demonstrated that NOP-1 significantly enhanced the glucose consumption capacity of insulin-resistant cells, exhibiting significant in vitro hypoglycemic activity.

The invention discloses a bolete soil covering device which comprises a vehicle plate, a steering moving assembly is installed on one side of the bottom of the vehicle plate, a walking assembly is installed on the other side of the bottom of the vehicle plate, a hand pushing frame is installed on one side of the vehicle plate, an assembling frame is installed on the top of the vehicle plate, and a soil pretreatment mechanism is installed on one side of the assembling frame. A soil conveying assembly is connected to the output end of the bottom of the soil pretreatment mechanism, a soil discharging groove is formed in the portion, corresponding to the output end of the soil conveying assembly, of the vehicle plate, a guide frame is installed at the bottom of the soil discharging groove, a first transmission mechanism is installed on one side of the soil pretreatment mechanism, and a second transmission mechanism is installed on one side of the soil conveying assembly. The other side of the first transmission mechanism and the other side of the second transmission mechanism are both installed on the walking assembly, a soil gathering assembly is installed at the position, on one side of the soil discharging groove, of the bottom of the vehicle plate, a drip irrigation assembly is installed on one side of the vehicle plate, the output end of the drip irrigation assembly is arranged at the bottom of the vehicle plate, and a compaction assembly is installed on the side, close to the walking assembly, of the bottom of the vehicle plate.