18 results about "Boletales" patented technology

The invention provides yellow-rose yellow-meat boletus fucomannan as well as a composition, a preparation method and application thereof, and belongs to the technical field of biological medicine mass health. The fucomannan has a structure represented by a general formula in an attached drawing of the abstract, and n represents the number of sugar repeating units and is a natural number. The weight-average molecular weight of the fucose glucomannan galactan of the boletus fucose is 10 kDa to 20 kDa, the polydispersity coefficient of the fucose glucomannan galactan is 1 to 2, and the molar ratio of glucose to mannose to fucose to galactose is 1.00 to 3.08 to 2.84 to 14.86. The boletus fucomannan and the composition containing the boletus fucomannan and the composition containing the boletus fucomannan and the composition containing the boletus fucomannan have the effects of promoting proliferation of beneficial bacteria, reducing the pH value of a microenvironment, increasing secretion of SCFAs and lactic acid, adjusting composition of intestinal microorganisms and the like, and have the function of improving the intestinal microecology. Therefore, the invention also discloses the fucomannan-glucomannan of the boletus roseus and the fucomannan-glucomannan of the boletus roseus and the fucomannan-glucomannan of the boletus roseus and the fucomannan-glucomannan of the boletus roseus and the fucomannan of the composition of the boletus roseus and the fucomannan of the composition of the boletus roseus and the fucomannan of the composition of the boletus roseus and the fucomannan of the composition of the composition of the composition of the composition of the composition of the composition of the composition of

The present application belongs to the technical field of application of fungal polysaccharides, and particularly relates to a Boletus edulis fruiting body polysaccharide as well as a preparation method and application thereof. Polysaccharides in Boletus edulis fruiting bodies are extracted, and the chemical structure of BDP-F is characterized by ultraviolet-visible light, Fourier transform infrared spectroscopy, one-dimensional nuclear magnetic resonance and two-dimensional nuclear magnetic resonance. The research results show that BDP-F is mainly composed of mannose, glucose, galactose and fucose. Methylation and nuclear magnetic resonance analysis show that the glycosidic bond of BDP-F is (1→6)-alpha-d-galactose, the main chain is composed of ->6)-alpha-d-galp-(1→ residues, and the O-2 position is branched by alpha-L-Fucp-(1→ and alpha-D-Manp-(1→ residues. Through DPPH free radical, hydroxyl radical and superoxide anion radical scavenging experiments, it is shown that BDP-F has the ability to serve as a natural antioxidant, and BDP-F can also reduce alcohol-induced GES-1 cell damage through antioxidant and anti-inflammatory effects within the experimental concentration range.

The application provides a method for removing protein from Boletus aereus polysaccharide, which comprises the following steps: crushing Boletus aereus fruiting bodies, adding an ethanol-ammonium sulfate aqueous two-phase system, and performing ultrasonic extraction; after standing and separation, collecting the water phase, concentrating, adding an ethanol solution, standing, centrifuging, taking the precipitate, and performing freeze-drying to obtain crude Boletus aereus polysaccharide; and performing deproteinization treatment on the crude Boletus aereus polysaccharide by using a cation exchange resin, collecting the eluent, concentrating, adding an ethanol solution, standing, centrifuging, taking the precipitate, and performing freeze-drying to obtain deproteinized Boletus aereus polysaccharide. The method for removing protein from Boletus aereus polysaccharide by using the ethanol-ammonium sulfate aqueous two-phase system for ultrasonic extraction in combination with the 732 cation exchange resin method has the advantages that the deproteinization rate of the extracted Boletus aereus polysaccharide is high, the polysaccharide retention rate is also high, the in-vitro activity of the polysaccharide is improved, and the method provides a research basis for in-depth development and utilization of Boletus aereus fungal resources to prepare functional food.

The invention belongs to the technical field of fungal polysaccharide application, and particularly relates to a yellow-rose yellow-meat boletus polysaccharide as well as a preparation method and application thereof. The preparation method comprises the following steps: extracting crude polysaccharide from boletus rosea sporocarp through a water extraction and alcohol precipitation method, and then carrying out deproteinization, decoloration and DEAE-52 cellulose column chromatography separation and purification to obtain the purified polysaccharide BPP-1 of the boletus rosea water component. CCl4 is selected as an inducer of oxidative stress injury of the MIHA cells to construct a hepatic cell oxidative injury model, and the model is utilized to evaluate the protective effect of BPP-1 on CCl4-induced MIHA cell hepatic injury. The result shows that the BPP-1 reduces the release of ALT and AST, improves the GSH level and the SOD enzyme activity at the same time, inhibits the generation of a lipid peroxidation product MDA, and effectively reduces the damage of CCl4 to liver cells. The results show that BPP-1 has a certain protection effect on CCl4-induced hepatocyte injury, and BPP-1 may be a potential antioxidant and can alleviate oxidative injury of liver.

The invention discloses a method for breeding by culturing sporocarp under pure culture conditions of boletus sinensis, and belongs to the technical field of edible mushroom breeding and cultivation. The method comprises the following steps: activating the boletus sinensis strain JSJ-Bx1, inoculating the boletus sinensis strain JSJ-Bx1 into a culture medium for dark culture, then performing low-temperature induction culture on an activated flat plate which is nearly overgrown with hyphae, changing the hyphae from white to golden yellow and generating hypha kinking, then transferring the hypha flat plate into a fruiting room for culture, and after obvious primordium differentiation is seen, inoculating the hyphae into a culture medium for culture, thereby obtaining the boletus sinensis strain JSJ-Bx1. Continuously performing induced culture to develop into mature sporocarp; sporocarp basidiospores are collected, diluted and coated in a B1 culture medium for germination culture, and monospora hyphae and polyspora hyphae are obtained and serve as stock seeds for breeding the boletus sinensis. According to the method, the mature sporocarp of the boletus sinensis can be directly and rapidly cultured in the agar culture medium, the harvested basidiospore can normally germinate and grow, and the whole life history of the boletus sinensis can be completed under the pure culture condition.

The invention relates to the technical field of fungus cultivation, in particular to a boletus edulis planting method with a high nutrition conversion rate. Comprising the following steps: fermenting bagasse to obtain fermented bagasse; uniformly mixing the crushed fermented bagasse and eucalyptus wood chips with soybean powder, oat powder and lime powder, and adding water until the water content reaches 50-55% to obtain a culture medium; the culture medium is bagged, sterilized and cooled, and the treated culture medium is obtained; inoculating a liquid strain of the boletus edulis into the treated culture medium, and culturing in a dark place to obtain hyphae of the boletus edulis; the method comprises the following steps: covering the surfaces of boletus edulis hyphae with rice field soil, controlling the temperature, humidity and illumination culture, then adjusting the temperature and humidity, and continuously culturing for 5-7 days to obtain the boletus edulis with high nutrition conversion rate. According to the planting method, the nutritional ingredients in the boletus edulis are effectively increased, the production cost of the boletus edulis is reduced, the strain culture time is shortened, the hybrid resistance is high, and the nutrition conversion rate is high.

The invention discloses a boletus aereus solid strain culture method, and relates to the technical field of microbial culture, the method comprises the following steps: preparing a special culture medium taking hard broadleaf sawdust with specific particle size and corncob as main materials, wheat bran and soybean meal as auxiliary materials, and mulberry twig fermentation dry powder as a biological activity induction component; putting the special solid culture medium into a culture bottle, sealing, sterilizing, and cooling for later use; inoculating the boletus aereus mother strain into the sterilized special solid culture medium; a micro-positive pressure intermittent ventilation culture process which is closed in the whole process and does not need punching is adopted for the culture bottle after culture and inoculation. The invention aims to solve the problems of high pollution risk, slow hypha growth and high planting failure rate caused by manual punching due to compact structure and poor air permeability of the existing solid culture gene, realizes the cooperation of high-porosity oxygen supply, precise nutrition supply and sterile environment guarantee, and remarkably improves the hypha activity and culture success rate. The method is suitable for efficient industrial production of boletus aereus solid strains.

This invention provides a composite polysaccharide-selenium nanoparticle complex, its preparation method, and its applications. The complex comprises polysaccharide-modified selenium nanoparticles, obtained by a redox reaction of a polysaccharide composition with a selenium solution. The polysaccharide composition includes *Boletus edulis* polysaccharide, *Portulaca oleracea* polysaccharide, and *Astragalus membranaceus* polysaccharide, with a mass ratio of 0.5–3:0.5–3:0.5–3. The composite polysaccharide-selenium nanoparticle complex of this invention, through the synergistic modification of selenium nanoparticles by multiple polysaccharides, effectively prevents the aggregation and oxidation of selenium nanoparticles, significantly improves the dispersibility and stability of selenium nanoparticles, enhances their bioavailability and targeting, strengthens their antioxidant activity, reduces their cytotoxicity, and achieves multifunctionality, expanding the application potential of selenium nanoparticles in medicine, food, cosmetics, and other fields.

The application discloses a method for cultivating fruit bodies of Chinese saprobic boletus under pure culture conditions, and belongs to the technical field of edible fungus breeding and cultivation. The method comprises the following steps: inoculating a Chinese saprobic boletus strain JSJ-Bx1 after activation into a culture medium for dark culture, then performing low-temperature induction culture on the plate with activated mycelium close to full growth, changing the mycelium from white to golden yellow, and causing mycelium kinking, then transferring the mycelium plate to a fruiting room for culture, continuing to induce culture to develop into mature fruit bodies after obvious primordium differentiation is observed, and collecting fruit body basidiospores, diluting and coating the basidiospores on a B1 culture medium for germination culture, obtaining pseudomonosporic mycelium and polysporic mycelium, and using the pseudomonosporic mycelium and polysporic mycelium as the original strain for Chinese saprobic boletus breeding. The application can directly and rapidly culture Chinese saprobic boletus mature fruit bodies on an agar culture medium, the harvested basidiospores can normally germinate and grow, and the Chinese saprobic boletus can complete the whole life history under pure culture conditions.

This invention discloses a strain of *Boletus urinaria* PP013 and its applications, with the preservation number CGMCC No. 40661. This invention also provides a sustainable, cyclical understory cultivation method, including liquid spawn culture, spawn culture, soil covering for fruiting, and understory inoculation. This method selects shallow-rooted tree species such as *Delonix regia* and *Gardenia jasminoides*, and lays a layer of dry weeds consisting of sugarcane bagasse, miscanthus, and *Digitaria sanguinalis* under the trees. Artificially cultivated fruiting bodies about to open their gills are placed under the trees as a live spore source for spore production. After spore production, the understory is covered with dry weeds and watered. The following year, after fruiting, some fruiting bodies are retained for natural spore production, achieving a single inoculation and multi-year cyclical fruiting. This invention creatively utilizes fruiting body spores for natural inoculation, replacing the traditional direct mycelial inoculation method. It boasts a high success rate of establishment, low long-term production costs, and represents a highly efficient forest-fungus integrated management model.

The present invention relates to a vegan leather containing any one mushroom mycelium selected from the group consisting of fungi in the Polyporaceae family, the Boletaceae family, and combinations thereof. Specifically, the present invention relates to: a vegan leather containing a mycelium derived from Trametes versicolor belonging to the Polyporaceae family or Retiboletus nigerrimus belonging to the Boletaceae family; and a method for manufacturing same. According to the present invention, the vegan leather can be manufactured using a mushroom strain, which is an eco-friendly material, rather than artificial leather or animal leather.

This invention belongs to the field of fungal polysaccharide application technology, specifically relating to a method for preparing and applying polysaccharide from *Boletus globosum*. Crude polysaccharide was extracted from the fruiting body of *Boletus globosum* using hot water extraction, and purified using DEAE-52 cellulose column chromatography. Subsequently, by establishing an alcohol-induced liver injury model, it was found that NOP-1 significantly reduced ALT and AST levels, while simultaneously decreasing MDA content and increasing SOD activity and GSH levels, indicating that NOP-1 can inhibit lipid peroxidation, enhance the antioxidant defense capacity of hepatocytes, and maintain hepatocyte membrane integrity. A HepG2 cell IR model was constructed using a high-glucose, high-insulin combined induction method, determining the optimal dosage of NOP-1. ‑6 Treatment of HepG2 cells with mol / L insulin for 24 h successfully induced insulin resistance. Glucose consumption experiments in IR HepG2 cells demonstrated that NOP-1 significantly enhanced the glucose consumption capacity of insulin-resistant cells, exhibiting significant in vitro hypoglycemic activity.

The invention provides a primer combination, a kit and a method for identifying boletus sinensis, and belongs to the technical field of boletus identification, the primer combination comprises a specific primer pair and an internal reference primer pair; the sequences of the specific primer pair are as shown in SEQ ID NO. 1 to SEQ ID NO. 2; the specific primer pair is used for amplifying a target sequence on a nuclear genome of boletus sinensis, and the target sequence is as shown in SEQ ID NO.3; the sequences of the internal reference primer pair are as shown in SEQ ID NO. 4 to SEQ ID NO. 5. The primer combination provided by the invention can be used for rapidly and accurately identifying the boletus sinensis.

The invention relates to a composition with anti-inflammatory and anti-allergic effects and application thereof. The composition is prepared from the following raw materials: bolete, emblic leafflower fruit and roxburgh rose. The composition with bolete, phyllanthus emblica and roxburgh rose as preparation raw materials is prepared by coupling steam explosion with heating reflux, the extraction efficiency and the biological activity are remarkably improved, and compared with a traditional extraction technology, the method also avoids use of organic reagents, and is more environmentally friendly and safer.

The invention belongs to the technical field of traditional Chinese medicine fermentation, and particularly relates to a fermentation product for improving liver depression fire or liver aging and a preparation method thereof. The fermentation product is prepared from the following raw materials in parts by weight: 20 to 40 parts of fructus corni, 15 to 30 parts of fructus citri sarcodactylis, 10 to 25 parts of bolete, 10 to 20 parts of rhizoma nelumbinis, 6 to 16 parts of an enzyme preparation and 0.11 to 0.25 part of a leavening agent. The traditional Chinese medicine fermentation product provided by the invention is prepared from four raw materials, namely dogwood, fingered citron, bolete and rhizoma nelumbinis, the four raw materials are synergistically matched and synergistically act on the liver from multiple aspects, and animal experiments find that the traditional Chinese medicine fermentation product can improve the inflammatory factor level of a rat model with liver depression transforming into fire, can improve the liver aging index of a rat liver aging model, and can improve the liver aging index of the rat liver aging model. According to the invention, the purpose of treating liver aging or liver depression transforming into fire is achieved through a liver aging index, a liver injury biochemical index and an oxidative stress level index.

The invention relates to a preparation method of a bolete extract as well as a product and application thereof, the preparation method comprises the following steps: drying bolete after steam explosion to obtain a dried bolete product; mixing the dried bolete product with water, and performing reflux extraction to obtain an extracting solution; concentrating the extracting solution to obtain a concentrated solution, mixing the concentrated solution with a flocculating agent, flocculating, filtering, and drying to obtain a finished product. According to the invention, an extraction method combining steam explosion and heating reflux is adopted, so that the extraction efficiency and biological activity of active components in the extract are remarkably improved.

The invention discloses a bolete soil covering device which comprises a vehicle plate, a steering moving assembly is installed on one side of the bottom of the vehicle plate, a walking assembly is installed on the other side of the bottom of the vehicle plate, a hand pushing frame is installed on one side of the vehicle plate, an assembling frame is installed on the top of the vehicle plate, and a soil pretreatment mechanism is installed on one side of the assembling frame. A soil conveying assembly is connected to the output end of the bottom of the soil pretreatment mechanism, a soil discharging groove is formed in the portion, corresponding to the output end of the soil conveying assembly, of the vehicle plate, a guide frame is installed at the bottom of the soil discharging groove, a first transmission mechanism is installed on one side of the soil pretreatment mechanism, and a second transmission mechanism is installed on one side of the soil conveying assembly. The other side of the first transmission mechanism and the other side of the second transmission mechanism are both installed on the walking assembly, a soil gathering assembly is installed at the position, on one side of the soil discharging groove, of the bottom of the vehicle plate, a drip irrigation assembly is installed on one side of the vehicle plate, the output end of the drip irrigation assembly is arranged at the bottom of the vehicle plate, and a compaction assembly is installed on the side, close to the walking assembly, of the bottom of the vehicle plate.

The invention discloses a method for inducing rapid formation of Chinese saprophytic boletus primordium, and belongs to the technical field of edible fungus cultivation. The method comprises the following steps: activating a boletus sinensis strain JSJ-Bx1, inoculating the boletus sinensis strain JSJ-Bx1 into a culture medium, and carrying out dark culture; the preservation number of the boletus sinensis strain JSJ-Bx1 is CGMCC (China General Microbiological Culture Collection Center) No.42007, and the boletus sinensis strain JSJ-Bx1 is preserved in the China General Microbiological Culture Collection Center; performing liquid fermentation culture on the hyphae which are activated and are almost overgrown on the flat plate to obtain fermentation liquid; inoculating a cultivation material with the fermentation liquid, performing dark culture until the bottle / bag is full of hyphae, covering the bottle / bag full of the hyphae with soil, covering the surface with non-woven fabric as a protective layer, and performing hypha culture and light culture for 3-5 days to obtain a soil-covered fruiting bottle / bag; performing light culture on the soil-covered fruiting bottle / bag in a fruiting room, and spraying a monopotassium phosphate solution during culture to promote mycelial growth and induce primordium formation. The method can effectively promote rapid growth of Chinese saprophytic boletus hyphae in an overburden layer, primordia are rapidly formed on the surface of the overburden layer, and harvesting can be performed 13-15 days after overburden.