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This invention belongs to the fields of biochemistry and medicinal chemistry. It discloses a medicinal and edible herbal compound based on Shanxi aged vinegar that helps regulate intestinal flora, its preparation method, and its application. The active ingredients are prepared from the following raw materials in parts by weight: 20-40 parts Shanxi aged vinegar, 10-15 parts Astragalus membranaceus, 6-10 parts Hawthorn berries, and 5-10 parts Poria cocos. This invention uses Shanxi aged vinegar as its core, combined with the herbal compound of Astragalus membranaceus, Poria cocos, and Hawthorn berries. For the first time, this invention scientifically combines Shanxi aged vinegar, a traditional fermented food, with classic spleen-strengthening and blood-activating herbs (Astragalus membranaceus, Hawthorn berries, and Poria cocos), embodying the wisdom of traditional Chinese medicine regarding "medicine and food sharing the same origin" and "strengthening the foundation and clearing the source." This produces a synergistic effect, breaking through the traditional production model and product form of Shanxi aged vinegar.