The invention discloses a method for extracting high-quality DNA (deoxyribonucleic acid) in dry leaves of soybeans suitable for transgenic detection. According to the method, extracted genomes are clear in DNA strips, high in purity and low in impurity content, and meet requirements for molecular biology experiments such as PCR (polymerase chain reaction), enzyme digestion, molecular cloning, sequencing and the like required by the transgenic detection. The method comprises the following steps of (1) soaking the dry leaves of the soybeans with Buffer I, heating at 40-60 DEG C, and cutting into powder; (2) adding Buffer II, shaking up, centrifuging, removing supernatant, repeating the operations until the supernatant is clarified, and taking precipitates; (3) adding a CTAB (cetyl trimethyl ammonium bromide) extracting solution, beta-mercaptoethanol of which the mass concentration is 3% and protease K, and heating for 40-60 minutes at 55-65 DEG C; (4) adding a mixed solution of phenol, chloroform and isoamyl alcohol equal in volume, uniformly mixing, centrifuging, and taking the supernatant; (5) adding a mixed solution of chloroform and isoamyl alcohol equal in volume, uniformly mixing, centrifuging, and taking the supernatant; and (6) adding pre-cooled isopropyl alcohol 0.8 times in volume, standing for 10-30 minutes at -20 DEG C, centrifuging, removing the supernatant, washing, airing, and dissolving in a TE buffering solution.