89 results about "Newcastle disease vaccine" patented technology

The invention discloses a gene VII-type newcastle disease virus strain with good immunogenicity and a low virulent strain subjected to passage attenuation through the newcastle disease virus strain. A newcastle disease virus strain F gene comprises a nucleotide sequence of a protein sequence shown by substantially-coded SEQ ID NO.2, and a virus strain HN gene comprises a nucleotide sequence of a protein sequence shown by substantially-coded SEQ ID NO.6. The newcastle disease virus strain is high in toxicity, and the growth rate on a chicken embryo is high; compared with a conventional newcastle disease virus strain, the gene VII-type newcastle disease virus strain has the advantages of being good in safety, high in immune protection capacity and immune efficacy and the like.

The invention relates to a preparation method of an inactivated newcastle disease vaccine. The preparation method comprises the steps of virus liquid inactivation, preparation of a compound traditional Chinese medicine extract, preparation of an aqueous phase, preparation of an oil phase and emulsification. According to the preparation method, the compound traditional Chinese medicine extract serving as an immunopotentiator is added to the inactivated newcastle disease vaccine so as to well cause cellular immunologic response and enhance the immunologic function of an organism, so that an antibody is generated in advance, and the antibody titer is obviously improved.

The invention relates to a traditional Chinese medicine immunopotentiator (an astragalus polysaccharide immunopotentiator for short) prepared from astragalus extracts and sulfated epimedium polysaccharides, which belongs to the field of immunological adjuvants of livestock and poultry. 1,000ml of the liquid medicine is prepared from 40g of astragalus and 120g of epimedium herb. The preparation method of the immunopotentiator comprises the following steps of: decocting the astragalus with water for three times, then merging the obtained filter liquor and condensing the filter liquor into 500ml of astragalus solution; extracting the epimedium polysaccharides by using the epimedium herb water decoction and ethanol precipitate method, then decorating the polysaccharides by using the chlorosulfonic acid-pyridine method, and preparing the polysaccharides into 500ml of sulfated epimedium polysaccharide solution after carrying out distilled water dialysis on the polysaccharides; and mixing the astragalus solution and the sulfated epimedium polysaccharide solution, and then filtering, sub-packaging and sterilizing to obtain the traditional Chinese medicine astragalus polysaccharide immunopotentiator. The immune experiment shows that the astragalus polysaccharide immunopotentiator has the advantage of obviously improving the proliferation of peripheral blood lymphocyte of chicken and enhancing the cellular immunity, obviously improving the potency of a serum antibody, promoting the lymphocyte proliferation, enhancing the cellular immunity and humoral immunity, and improving the immune response of a vaccine by coordinately immunizing chickling by using the Newcastle disease vaccine.

The present invention concerns cDNAs for making attentuated, infectious Newcastle disease virus (NDV). Another aspect of the invention relates to methods of making the cDNAs. Another aspect of the invention is a vector containing the cDNA optionally linked to an operable promoter. Within the scope of the invention are vaccines comprising the attenuated, infectious NDV. Also disclosed are methods of making the vaccines and methods of using the vaccines to prevent or treat Newcastle disease in an avian host. The present invention also concerns the nucleotide sequences of the entire genome of NDV, the leading region, the trailing region, and the NP region, as well as proteins encoded by these nucleotide sequences.

The invention discloses a heat-resisting and freeze-drying protector for a live vaccine and a preparation method thereof as well as a live newcastle disease vaccine adopting the freeze-drying protector. The heat-resisting and freeze-drying protector for the live vaccine comprises the following compositions in percentage by weight: 10-15 percent of trehalose, 1-2 percent of polyvinylpyrrolidone (PVP), 1-2 percent of alum, 1-2 percent of glutamine, 2-10 percent of sorbitol, 0.1-0.5 percent of potassium dihydrogen phosphate, 0.5-1 percent of vitamin C and the balance of water. Compared with the traditional vaccine, the heat-resisting and freeze-drying protector for the live vaccine has the advantages of good stability, long preservation time, low preservation condition, little activity damage on the live vaccine, good dissolubility, simple raw material and the like.

The invention relates to a traditional Chinese medicine compound preparation for enhancing livestock immunity, which comprises 1-5 parts of Radix Astragali, 1-5 parts of Radix Codonopsis and 1-5 parts of ligustrum lucidum, and the application forms can be a traditional Chinese medicine powder, an oral liquid, a particulate agent and an injection. The traditional Chinese medicine compound preparation for enhancing livestock immunity can be used for enhancing the livestock immunity functions of chicken, ducks, gooses, pigs and sheep; and especially, can be used for enhancing the immunization effect of vaccines such as newcastle disease vaccine or hog cholera vaccine. The traditional Chinese medicine compound preparation before and after the animal immunization and the vaccine are simultaneously used, the immunization function of animal body can be enhanced, and the immunization effect of the vaccine can be enhanced.

The invention relates to recombinant newcastle disease vaccine candidate rAI4-penton to express avian adenovirus penton protein and a construction method thereof. newcastle viral strain rAI4-penton iscollected under CGMCC No: 15492. The construction method includes: using a reconstructed reverse genetics operating platform of a newcastle attenuated strain to penton gene sequence of avian adenovirus into genome full-length transcription vector pNDV/rAI4 of the strain AI4 so as to obtain recombinant newcastle viral genome full-length cDNA clone pNDV/rAI4-penton, and performing transfecting to obtain recombinant viral rAI4-penton that successfully express penton protein. The recombinant virus rAI4-penton has high breeding titer on chicken embryos, can stabilize the penton protein even aftercontinuous passage, is suitable for large-scale production of vaccines, and is suitable for making vaccines.

The invention relates to the technical field of Newcastle disease vaccine in particular to Newcastle disease virus-like particles, a preparation method and a patent application of an application thereof. The virus-like particles are protein particles with a hollow structure formed by the self-assembly of Newcastle disease virus M protein, F protein, NP protein and HN protein. The base sequence of a M1 gene corresponding to the M protein is shown in SEQ ID NO.1, the base sequence of the F1 gene corresponding to the F protein is shown in SEQ ID NO.2, the base sequence of the NP1 gene corresponding to the NP protein is shown in SEQ ID NO.3, the base sequence of the HN1 gene corresponding to the HN protein is shown in SEQ ID NO. 4. The Newcastle disease virus-like particles, preparation method and application have the technical advantages of high expression level of structural protein, self-assembly of the virus-like particles, immunogenicity closer to real virus particles, high yield and purity of the virus-like particles, and good immunization effect.

The invention discloses a preparation method of chitosan newcastle disease vaccine nanoparticles, and relates to the preparation method of the chitosan newcastle disease vaccine nanoparticles. The method is that: 1, the chitosan is dispersed in the sterilized distilled water, under the magnetic stirring, the acetic acid is added to be stirred, filtered and carried out standing until the bubbles are eliminated, i.e. the chitosan solution is obtained; 2, the chitosan solution is taken and is dripped with the newcastle disease L-series virus liquid to be stirred and mixed uniformly, then the cross-linking agent is dripped, then the solution A is obtained after being stirred under normal temperature and aseptic conditions; and 3, the solution A undergoes centrifugal treatment, the sediment is taken out, is washed by sterile water, and then is vacuum freezed and dried, i.e. the chitosan newcastle disease vaccine nanoparticles are obtained. The vaccine nanoparticles has strong stability and long medicine release time, under the premise of maintaining the drug effect, the dosage is reduced, the toxic reaction side effect is reduced or avoided, and the immune protection period can be prolonged.

The invention relates to establishment of a hybridoma cell strain for secreting duck NDV (newcastle disease virus)-resisting isolate monoclonal antibody, which belongs to the technical field of molecular immunology and virology. The purified HN protein expressed by duck NDV isolate SDO3 pronucleus is used as immunogen, one hybridoma cell strain capable of secreting duck NDV-resisting isolate monoclonal antibody is researched, and the preservation number is CCTCC C2013173. The NDV specificity monoclonal antibody secreted by the hybridoma cell strain cannot be specially combined with the NDV strain but can be specifically combined with NDV clinical wild strain, so that the NDV specificity monoclonal antibody can be used as an identification reagent to be used for identifying the NDV vaccine virus and clinical wild strain, and the specificity is strong; the sensitivity is 1: 212 HAU; the hybridoma cell strain has the advantage of high sensitivity.

The invention relates to an application of paecilomyces cicadae cordyceps polysaccharides in preparation of a chicken immunity enhancement reagent and belongs to the field of immunologic adjuvants for livestock and poultry. The paecilomyces cicadae cordyceps polysaccharides are prepared from paecilomyces cicadae sporophore or a culture medium through heat reflux extraction and later separation and purification by the aid of a ceramic membrane. The technology is simple, and immunity tests prove that the immunologic function of the chicken organisms can be remarkably improved by the immunity enhancement reagent containing the paecilomyces cicadae cordyceps polysaccharides; through matching with a new castle disease vaccine for chicken immunization, the immune response of the vaccine can be remarkably improved, the immunization lasts for a longer time, and the preparation is free of drug residues, toxic and side effects and the like, and is an environment-friendly, efficient and stable chicken immunity enhancement reagent.

The invention relates to triad inactivated vaccine for newcastle disease, bird flu (H9 subtype) and escherichia coli disease and a preparation method thereof. The vaccine is an effective coping strategy aiming at a prevalence situation of mixed infection of the newcastle disease, the H9 subtype bird flu and the escherichia coli, and two kinds of disease can be controlled at the same by means of immunization at one time. According to the vaccine, a La Sota strain of a newcastle disease virus, a LG1 strain of an H9 subtype bird flu virus, a QL1 strain (O1) of bird source escherichia coli, QL2 strain (O2) of the bird source escherichia coli, QL78 strain (O78) of the bird source escherichia coli, and QL36 strain (O36) of the bird source escherichia coli serve as vaccine reserve virus strains, and the vaccine is formed through antigen liquid preparation, inactivation, concentration and mixing according to a certain proportion. The vaccine and the preparation method are related with the La Sota strain of the newcastle disease virus, the H9 subtype bird flu virus and the bird source escherichia coli which are all domestic prevalence virus strains, and current prevalence disease can be effectively prevented.

The invention aims to prepare a chicken subunit four-combination vaccine which is capable of simultaneously preventing newcastle disease, infectious bronchitis, avian influenza, infectious bursal disease and particularly super-strong virus infection. The vaccine comprises infectious bursal disease VP2 protein with the sequence of SEQ ID NO:1, newcastle disease virus, infectious bronchitis virus and avian influenza virus. The four-combination vaccine can be applied to vaccine immunity of chickens of 21 days, can be used for simultaneously preventing the newcastle disease, the infectious bronchitis, the avian influenza and the infectious bursal disease, has immune effects similar to those of a single newcastle disease vaccine, the inactivated vaccine of the infectious bronchitis, the inactivated vaccine of the avian influenza and single infectious bursal disease vaccine, and fulfills the aims of reducing immunization frequency and lowering stress.

A traditional Chinese medicine immunopotentiator prepared from mulberry leaves polysaccharide and eucommia polysaccharide. The immunopotentiator can stimulate proliferation of chicken lymphocytes in vitro. When used together with newcastle disease vaccine to immunize chickens, the immunopotentiator can increase serum antibody titer, promote proliferation of lymphocytes, and enhance cellular immunity and humoral immunity of the chickens. When used together with porcine productive and respiratory syndrome vaccine to immunize piglets, the immunopotentiator can increase the serum antibody titer. When used together with the porcine productive and respiratory syndrome vaccine to immunize layers, the immunopotentiator can increase porcine productive and respiratory syndrome virus yolk antibody titer and improve immune effects of the vaccine.

The invention relates to an application of chitosan in avian vaccine composition preparation. An avian vaccine composition comprises newcastle disease inactivation virus or avian influenza inactivation virus and other avian vaccine antigens, and a chitosan solution, wherein the avian vaccine comprises a water phase and an oil phase, the chitosan solution is in the water phase, and a concentration of the chitosan solution is 0.1-0.5% W/V, most preferably 0.2-0.5% W/V. According to the avian vaccine composition, the amount of the antigen is low so as to reduce side reactions of the avian vaccine and reduce avian vaccine cost, such that the avian vaccine composition such as newcastle disease vaccines or avian influenza vaccines and the like are suitable for industrial production.

The invention discloses an inactivated vaccine prepared by a Newcastle disease virus heat stable strain and a preparation method thereof. The vaccine takes the Newcastle disease virus heat stable strain as a vaccine virus seed. The preparation method comprises the following steps: preparing viral allantoic fluid of the Newcastle disease virus heat stable strain, diluting the viral allantoic fluidwith optimized dilution buffer, and preparing and inspecting beta-propiolactone and oil-adjuvant inactivated vaccine. The heat stability of the vaccine is obviously higher than that of the other conventional inactivated vaccines, and the inactivated vaccine also has excellent immunogenicity, solves the problems of relatively poor stability, high dependency level on a cold chain system and the likein the conventional common Newcastle disease virus vaccine, and has wide application prospects.

The invention discloses a breeding method for white feather hybrid broilers. The breeding method comprises the following parts: 1, temperature and humidity control: controlling the temperature to be 35-37 DEG C when the white feather hybrid broilers are 1-7 days old, decreasing the temperature for 1-2 DEG C every week afterwards, and controlling the temperature to be 25-30 DEG C when the white feather hybrid broilers are 6-10 weeks old; controlling relative humidity to be 60-70 percent before the white feather hybrid broilers are 20 days old, and controlling the relative humidity to be 50-60 percent from that the white feather hybrid broilers are 20 days old until slaughtered; 2, nutritional requirements: feeding with 20 percent of complete pellet feed protein when the white feather hybrid broilers are 1-25 days old, and feeding with 17 percent of complete pellet feed protein from that the white feather hybrid broilers are 25 days old until slaughtered; 3, vaccine epidemic prevention: performing the subcutaneous injection of Newcastle disease vaccine, infectious bronchitis bivalent attenuated nasal drip and eye drop vaccine and avian influenza H9, the injection of bursa fabricius attenuated vaccine and the subcutaneous injection of avian influenza H5 inactivated vaccine, and enabling the white feather hybrid broilers to have Newcastle disease IV-series attenuated vaccine drinking water or performing the intramuscular injection of Newcastle disease IV-series attenuated vaccine. The white feather hybrid broilers bred by using the technical method have the advantages that the growth rate is 7-10 days higher than that of the original breed, the disease resistance is strong, the usage amount of a medicine is reduced, the feed conversion rate is high, and can be reduced by 0.1-0.2 percent, the survival rate of the white feather hybrid broilers is 2-3 percent point higher than that of the original breed, and the like. The white feather hybrid broilers are red in crown and bright in feather from the appearance, naturally yellow in feet, skin and mouth, and tender in meat quality, suitable for flavor cooking in all regions.

An animal immunopotentiator, cytokine adjuvant-chicken Newcastle disease vaccine (cc strain) is prepared by coordination of the cytokine (thymopeptide or transfer factor) with low-virulent immunoactivator of Newcastle disease.

The invention provides a method for preparing a vaccine by Newcastle disease virus cultured by using a chick embryo continuous cell line and a bioreactor, and belongs to the technical field of animal biological products. The method comprises the following steps: 1) performing passage and culture on a master cell; 2) multiplying a cell-adapted virus seed; 3) culturing the cell for multiplying virus in a suspending manner; 4) culturing and harvesting the virus; 5) preparing a chick Newcastle disease live vaccine; and 6) preparing the chick Newcastle disease live vaccine. The method adopted for preparing vaccines is simple and stable in production process, high in virus content, small in differences among batches, is easy to operate and control the quality, and can remarkably improve the yield and quality of vaccines; the produced chick Newcastle disease vaccines with high safety and high immune efficacy have a complete immune protection effect on the attack of virulent Newcastle disease virus.

The invention relates to a chimeric Newcastle disease virus vector H7 (avian influenza) live vaccine candidate strain capable of overcoming an effect of a Newcastle disease virus maternal antibody of a chick and a construction method thereof. The preservation number of the Newcastle disease vaccine candidate strain is CGMCC No.13798. The construction method includes: making use of a reverse genetic manipulation platform of a chimeric Newcastle disease virus rAI4-TFHN which is capable of being replicated in the existence of a Newcastle disease antibody, inserting an HA (hemagglutinin) sequence of a subtype H7N9 (avian influenza) virus strain into a full-length transcription vector of an AI4-T4FHN strain genome so as to obtain a full-length cDNA (complementary deoxyribonucleic acid) clone pNDV/rAI4-T4FHN-H7 of a recombinant Newcastle disease virus genome containing the subtype HA gene of the H7N9 (avian influenza) virus. The recombinant virus AI4-T4FHN-H7 obtained through transfection is high in propagation titer in chick embryos, still capable of expressing HA protein stably after continuous passage and applicable to large-scale production of vaccine and can be used for manufacturing the vaccine.

The invention relates to a preparation method and application of atractylodes macrocephalaon polysaccharide-bacillus fermentation liquor. The preparation method includes the steps of inoculating activated bacillus subtilis to an atractylodes macrocephalaon polysaccharide liquid medium, and performing ferment culturing to obtain the atractylodes macrocephalaon polysaccharide-bacillus fermentation liquor. The atractylodes macrocephalaon polysaccharide-bacillus fermentation liquor is capable of enhancing animal immunity and improving animal disease resistance and vaccine protection rate, has more remarkable effect especially while being used with Newcastle disease vaccines and thus is highly worthy of popularization in preventing and controlling animal infectious diseases.

The invention discloses an application of maggot extract in preparing medicament that enhances immunity of inactivated vaccine for pigeon Newcastle disease. According to the invention, live maggots are induced through supersonic wave, bodies are crashed to obtain extract as an additive to pigeon forge or drinking water, and immunization effect of pigeon Newcastle disease vaccine is improved, therefore immunity against pigeon pest is enhanced. Through the immunopotentiation of the maggot, protection rate of Newcastle disease vaccine is improved, use of antiviral drug is greatly reduce, which is beneficial for production of nuisance-free foodstuff and the effective rate is up to 100%.