Preparation method and application of atractylodes macrocephalaon polysaccharide-bacillus fermentation liquor

A technology of Atractylodes macrocephala polysaccharides and Bacillus, applied in the field of livestock and poultry immunity, can solve the problems of lack of immune enhancers, short duration of curative effect, long duration of medication, etc., to achieve the goal of enhancing animal immune function, great promotion value, and improving disease resistance Effect

Inactive Publication Date: 2015-12-02
SHANDONG ENKANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the shortcomings of chemical drugs such as long medication time, short duration of curative effect, drug residues, and environmental pollution, and the immune response of animals has an extremely complicated

Method used

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  • Preparation method and application of atractylodes macrocephalaon polysaccharide-bacillus fermentation liquor
  • Preparation method and application of atractylodes macrocephalaon polysaccharide-bacillus fermentation liquor
  • Preparation method and application of atractylodes macrocephalaon polysaccharide-bacillus fermentation liquor

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Experimental program
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Effect test

Embodiment 2

[0032] The Bacillus subtilis in Example 2 and Example 3 is derived from the General Microorganism Center of China Committee for Culture Collection of Microorganisms, and the strain preservation number is CGMCCNO: 1.4255;

Embodiment 4

[0033] The Bacillus subtilis in Example 4 is derived from the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the strain preservation number is CGMCCNO: 1.3382;

[0034] Atractylodes macrocephala was purchased from Yongchuntang Pharmacy in Tai'an City;

[0035] The broth was prepared as follows:

[0036] Peptone 10g, beef extract 3g, sodium chloride 5g, distilled water to a volume of 1000mL, after boiling, adjust the pH to 7.2-7.4, and autoclave at 121°C for 15min.

Embodiment 1

[0037] The preparation of embodiment 1 Atractylodes macrocephala polysaccharide

[0038] Based on 1000 g of Atractylodes macrocephala, add 8 times the mass of water and soak for 30 minutes; then boil for 1 hour, add 6 times the mass of water to the filtered medicinal dregs and boil for 1 hour, add 6 times the mass of water to the filtered residue and boil for 1 hour to filter, combine the three times of filtrate, and The filtrate was heated and concentrated to 1000mL, and after cooling, it was centrifuged at 3000rpm for 10min to remove impurities, and distilled water was fixed to make 1000mL; it was extracted by one-step alcohol precipitation method, and 95wt% ethanol was slowly added to the above concentrated solution to make the final concentration of ethanol in the mixture reach 80wt% , stirred while adding, after the addition was completed, let it stand for 24 hours, centrifuged, took the precipitate and dried it in a 60°C oven to obtain Atractylodes polysaccharide.

[003...

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Abstract

The invention relates to a preparation method and application of atractylodes macrocephalaon polysaccharide-bacillus fermentation liquor. The preparation method includes the steps of inoculating activated bacillus subtilis to an atractylodes macrocephalaon polysaccharide liquid medium, and performing ferment culturing to obtain the atractylodes macrocephalaon polysaccharide-bacillus fermentation liquor. The atractylodes macrocephalaon polysaccharide-bacillus fermentation liquor is capable of enhancing animal immunity and improving animal disease resistance and vaccine protection rate, has more remarkable effect especially while being used with Newcastle disease vaccines and thus is highly worthy of popularization in preventing and controlling animal infectious diseases.

Description

technical field [0001] The invention relates to a preparation method and application of Atractylodes polysaccharide-Bacillus fermented liquid, in particular to a method for preparing fermented liquid by fermenting Atractylodes polysaccharide-Bacillus and the application of the fermented liquid to enhance the immunity of livestock and poultry. Field of poultry immunization technology. Background technique [0002] With the development of China's economy and the rapid growth of animal husbandry, animal diseases not only cause huge economic losses to animal husbandry production, but also some zoonotic diseases directly threaten the survival and development of human beings. It is now recognized that disorders of the body's immune system are more susceptible to infection. However, due to the shortcomings of chemical drugs such as long medication time, short duration of curative effect, drug residues, and environmental pollution, and the immune response of animals has an extremel...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04A23K1/16
Inventor 赵晓娜陈抗李刚付荣强赵彦孙明珠陈芳
Owner SHANDONG ENKANG PHARMA
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