The invention discloses a preparation process of sika deer penis wine, and relates to the field of making of medicinal wine. A preparation process of the sika deer penis wine comprises the following steps of S1, pretreating raw materials; S2, screening and crushing the pretreated raw materials; S3, performing primary soaking; S4, performing secondary soaking; S5, performing distilling and purifying; S6, performing comprehensive treatment; and S7, performing blending. According to the scheme, the utilization and absorption rate of effective components is increased, the raw materials are filtered and then purified in the liquid distillation and purification process, the drinking experience feeling of the sika deer penis wine is improved, the processing difficulty is reduced, the processing work efficiency is improved, and through combined soaking of multiple medicinal materials, the sika deer penis wine contains medicinal components for tonifying the kidney and strengthening essence, andhas the efficacy of nourishing the kidney and strengthening the essence. Moreover, the sika deer penis wine is beneficial to absorption by a human body, and has a good health-care efficacy on the human body, the manufacturing period is shortened, the manufacturing efficiency is improved, and the sika deer penis wine is convenient to sell, and can be blended in different proportions according to the required health-care efficacy, so that the health-care effect is clearer.