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Method for producing medium-chain fatty acid through fermentation of exogenous medium enhanced anaerobic microorganisms

A technology of anaerobic microorganisms and medium-chain fatty acids, which is applied in the production of medium-chain fatty acids, and can solve problems such as low solubility, low gas utilization efficiency, and slow gas-liquid mass transfer rate

Active Publication Date: 2020-11-10
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The solubility of CO and H2 in water is low, and the gas-liquid mass transfer rate is slow, which makes the problem of low gas utilization efficiency common in the synthesis gas fermentation process

Method used

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  • Method for producing medium-chain fatty acid through fermentation of exogenous medium enhanced anaerobic microorganisms
  • Method for producing medium-chain fatty acid through fermentation of exogenous medium enhanced anaerobic microorganisms
  • Method for producing medium-chain fatty acid through fermentation of exogenous medium enhanced anaerobic microorganisms

Examples

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Effect test

Embodiment 1

[0097] A method for strengthening anaerobic microbial fermentation to produce medium-chain fatty acids with an exogenous medium, comprising the steps of:

[0098] 1) Weigh chloroplatinic acid, dissolve it in ethylene glycol, and stir to make it dissolve evenly;

[0099] 2) Weigh Fe 2 o 3 powder, drop the solution obtained in step 1) into Fe 2 o 3 in, stir to mix thoroughly;

[0100] 3) Transfer the material prepared in step 2) to a drying oven, and dry it in an air atmosphere at 150°C for 12 hours;

[0101] 4) After drying in step 3), the solid powder is heated at 350°C using H 2 The iron-loaded platinum solid medium can be obtained by reduction reaction in syngas with a volume ratio of 1:1 for 10 hours;

[0102] 5) Put the activated sludge sterilized by high pressure steam at 120°C for 60min into the anaerobic fermentation reactor, and add the necessary medium; 2 After long-term cultivation and domestication under the environmental conditions of / CO (1atm), anaerobic a...

Embodiment 2

[0118] A method for strengthening anaerobic microbial fermentation to produce medium-chain fatty acids with an exogenous medium, comprising the steps of:

[0119] 1) Weigh the dinitrosodiammine platinum nitric acid solution, dissolve the dinitrosodiammine platinum nitric acid solution in water, stir to make it dissolve evenly, and obtain a colorless, clear and transparent solution;

[0120] 2) Weigh Fe 3 o 4 powder, drop the solution obtained in step 1) into Fe 3 o 4 in, stir to mix thoroughly;

[0121] 3) Transfer the product prepared in step 2) to a drying oven, and dry it in an air atmosphere at 120°C for 10 hours;

[0122] 4) After drying in step 3), the solid powder is heated at 300°C using H 2 Iron-loaded platinum solid medium can be obtained by reduction in syngas with a volume ratio of 1:1 for 10 hours;

[0123] 5) Put the activated sludge sterilized by high-pressure steam at 120°C for 60 minutes into the anaerobic fermentation reactor, and add necessary nutrient...

Embodiment 3

[0138] A method for strengthening anaerobic microbial fermentation to produce medium-chain fatty acids with an exogenous medium, comprising the steps of:

[0139] 1) take palladium nitrate solution, dissolve palladium nitrate solution in water, stir to make it dissolve evenly;

[0140] 2) Weigh MoO 3 powder, drop the solution obtained in step 1) into MoO 3 in, stir to mix thoroughly;

[0141] 3) Transfer the material prepared in step 2) to a drying oven, and dry in an air atmosphere at 120°C-180°C for 8-12h to obtain a solid;

[0142] 4) Weigh trisodium citrate, dissolve it in water, stir to make it dissolve evenly, and obtain a colorless and transparent solution; drop the obtained colorless and transparent solution into the solid obtained in step 3), stir to make it fully mixed, and obtain solid medium precursor;

[0143]5) Transfer the solid medium precursor obtained in step 4) to a drying oven, and dry it in an air atmosphere at 120°C-180°C for 8-12h;

[0144] 6) The s...

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Abstract

The invention discloses a method for producing medium-chain fatty acid through fermentation of exogenous medium enhanced anaerobic microorganisms. The method comprises the following steps of pretreating an exogenous medium, and performing grinding to obtain exogenous medium solid particles; pretreating anaerobic microorganism inoculum and performing inoculating into a culture medium; inflating synthesis gas into the culture medium, culturing and domesticating anaerobic microorganisms, and performing enriching to obtain anaerobic acid-producing microorganisms capable of realizing carbon chain extension; mechanically and physically mixing the exogenous medium solid particles with the domesticated anaerobic acid-producing microorganisms under an anaerobic condition to obtain a mixed fermentation system for producing medium-chain fatty acid; and inflating synthesis gas into a reactor to obtain the medium-chain fatty acid. According to the method, the anaerobic microorganisms and the exogenous medium form a mixed fermentation system together, and the fermentation system can accelerate the carbon chain extension process of the anaerobic acid-producing microorganisms to produce the medium-chain fatty acid. The fermentation system can maintain stable operation for 2-36 months, an exogenous medium is not liable to inactivate, and anaerobic microorganism death is not liable to cause.

Description

technical field [0001] The invention relates to a production method of medium-chain fatty acid. More specifically, it relates to a method for enhancing anaerobic microbial fermentation to produce medium-chain fatty acids with an exogenous medium. Background technique [0002] In recent years, the demand for and dependence on energy in countries around the world has increased dramatically, and fossil energy has become increasingly scarce. At the same time, in order to achieve the sustainable development of the society and the health and stability of the ecosystem, the production of renewable energy and chemicals by clean and environmentally friendly means has received great attention. Anaerobic fermentation, as a new type of biotechnology with the advantages of high load, low energy consumption, low operating cost and energy generation, can not only replace traditional landfill and incineration waste treatment methods, but also recover clean energy such as methane. At the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/40C12P7/52C12P7/54C12N1/36
CPCC12P7/40C12P7/52C12P7/54C12N1/36Y02E50/30
Inventor 王雯刘昊鹏张燚马铭锐刘广青杨紫怡苏本生
Owner BEIJING UNIV OF CHEM TECH
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