141 results about "Anaeromicrobium sediminis" patented technology

A media supplement for culturing anaerobic bacteria is provided which comprises a filtrate of effluent from a chemostat vessel in which a target bacterial ecosystem has been cultured. Methods of using the supplement for culturing or isolating anaerobic microbial strains or communities, particularly anaerobic bacteria from the human gut, are also provided.

A stable method for producing liquid products such as ethanol, propanol, butanol and other chemicals from syngas components that contacts CO or a mixture of CO2 and H2 with a highly porous side of an asymmetric membrane under anaerobic conditions and transfers these components into contact with microorganisms contained within bio-pores of the membrane. A liquid contacting side of the membrane utilizes a dense layer to control hydration of the bio-pores with a liquid phase. The gas feed directly contacts the microorganisms in the bio-pores and maximizes their utilization of the syngas. Metabolic products produced by the microorganisms leave the membrane through the side opposite the entering syngas. This method establishes a unitary direction across the membrane for the supply of the primary feed source to the microorganisms and the withdrawal of metabolically produced products. The feed and product flow improves productivity and performance of the microorganism and the membrane.

A method of producing in a bioreactor a biogas rich in methane involves electrolyzing water in an aqueous medium at a voltage in a range of from 1.8 V to 12 V in the presence of electrochemically active anaerobic microorganisms that biocatalyze production of hydrogen gas, and, contacting a species of hydrogenotrophic methanogenic microorganisms with the hydrogen gas and carbon dioxide to produce methane. Volumetric power consumption is in a range of from 0.03 Wh / LR to 0.3 Wh / LR. Current density is 0.01 A / cmE2 or lower. The voltage is sufficient to electrolyze water without destroying microbial growth. Such a method results in improved electrolysis efficiency while avoiding the use of noble metal catalysts. Further, a combination of water electrolysis with anaerobic degradation of organic matter results in increased biogas quality and in increased biogas quantity and yield. Oxidation of hydrogen sulfide contributes to the increased quality, while an increase in the rate of organic matter hydrolysis and an increase in the production of methane from hydrogen contributes to the increased quantity and yield.

The invention relates to a method for producing a clean energy source by a continuous feeding type anaerobic fermentation method, particularly to a continuous feeding type anaerobic fermentation method for preparing methane by fermenting catering organic waste. The method for producing the clean energy source through continuous feeding type anaerobic fermentation can be widely used for preparing the methane which is a clean energy source through the anaerobic fermentation of the catering organic waste, straws, animal excreta in farms and other organic waste. The method comprises the following steps of: draining partial moisture of the collected catering organic waste to enable the water content thereof to be not more than 80 percent, and then adding the processed catering organic waste and acclimated anaerobic activated sludge in a definite proportion into a reactor for fermentation. The catering organic waste is added into the reactor by adopting a continuous feeding method in the fermentation process, and a fermentation raw material is added once at regular intervals. An addition period is a gas production vigorous period. The addition is 0.1-10 percent of the volume of the reactor. An anaerobic state is strictly controlled in the whole process so as to facilitate the growth of anaerobes.

The invention discloses a method for producing medium-chain fatty acid through fermentation of exogenous medium enhanced anaerobic microorganisms. The method comprises the following steps of pretreating an exogenous medium, and performing grinding to obtain exogenous medium solid particles; pretreating anaerobic microorganism inoculum and performing inoculating into a culture medium; inflating synthesis gas into the culture medium, culturing and domesticating anaerobic microorganisms, and performing enriching to obtain anaerobic acid-producing microorganisms capable of realizing carbon chain extension; mechanically and physically mixing the exogenous medium solid particles with the domesticated anaerobic acid-producing microorganisms under an anaerobic condition to obtain a mixed fermentation system for producing medium-chain fatty acid; and inflating synthesis gas into a reactor to obtain the medium-chain fatty acid. According to the method, the anaerobic microorganisms and the exogenous medium form a mixed fermentation system together, and the fermentation system can accelerate the carbon chain extension process of the anaerobic acid-producing microorganisms to produce the medium-chain fatty acid. The fermentation system can maintain stable operation for 2-36 months, an exogenous medium is not liable to inactivate, and anaerobic microorganism death is not liable to cause.

The invention discloses an upflow blanket filter (UBF) anaerobic reactor with a filter layer on the top and for treating chemical wastewater. The UBF anaerobic reactor comprises a reactor, a water distribution device, a sludge expanded bed, a three-phase separator, an effluent collecting device and a methane circulating device and is characterized in that the three-phase separator is arranged in the middle of the reactor; the sludge expanded bed is arranged at the lower part of the three-phase separator; a biological filling region is arranged at the upper part of the three-phase separator; the biological filling region comprises filling and a biological membrane; and the biological membrane is formed by attaching anaerobic microorganisms to the surface of the filling. The UBF anaerobic reactor is suitable for treating the chemical wastewater which has high salt content and low suspended matter content and is difficult to degrade; and gas lift internal circulation is adopted, namely methane generated in the reactor is collected by the three-phase separator and internal circulation is realized by using the methane as the lifting powder and without external mechanical power.

The invention relates to a wastewater treatment process with an effect of coupling nano magnetite for enhancing the activity of anaerobic microorganisms, and aims at solving the problems that a traditional anaerobic biological treatment system is easy to acidify, instable in performance and the like. Nano magnetite of which the average particle size is smaller than 100nm is prepared by the steps of dissolving ferric salts, precipitating, separating, washing and the like; the nano magnetite and anaerobic sludge are sufficiently and uniformly mixed at a proportion of 1.55gFe / g MLVSS (Mixed Liquor Volatile Suspended Solid) by a stirring method, a nitrogen blowing method and the like; a mixture is cultured by an anaerobic organism treatment device under the constant temperature condition of 35DEG C; with good electrical conductivity and high specific surface area of the nano magnetite, interspecific electron transfer between the anaerobic microorganisms is remarkably enhanced, the activity of the anaerobic microorganisms is greatly improved, and finally, the performance of an anaerobic organism treatment process is improved.

The invention provides a plant root layered membrane-forming enhanced water body repairing method for biological denitrification, and relates to the technical field of the biological denitrification of surface water. The invention provides the plant root layered membrane-forming enhanced water body repairing method for the biological denitrification, which comprises the following steps of: based on the conventional ecological floating island and floating bed, adequately using the developed plant root system to be taken as a carrier for growing and breeding denitrified microorganism, respectively carrying out the layered fixation of anaerobic denitrifying bacteria and aerobic denitrifying bacteria, and adequately using the plant root exudates to be taken as a denitrification carbon source, so that the aim of the biological denitrification of the enhanced water body can be achieved. According to the method, after the plant is acclimatized and formed with the membrane in a layered way, the part which is nearer to the root region forms into an anoxic region which is applicable to the growing and the breeding of the facultative or anaerobic microorganism, to promote the anaerobic denitrification of the microorganism; and the part which is further away from the root region forms into an aerobic region which is applicable to the growing and the breeding of the aerobic microorganism, to promote the nitratlon reaction of the microorganism.

The present invention is directed to a medium, broth or agar, and a method of utilizing the same, in order to isolate and / or identify anaerobes from a mixed sample that contains facultative microorganisms. The medium contains an inhibitor of the electron transport system, such as a salt of azide (N3−), cyanide (CN−) or related compounds. These inhibitors are present in an amount sufficient to limit the growth of facultative microorganisms under anaerobic conditions while not inhibiting the growth of the anaerobe microorganisms. Preferably, the inhibitor is present in the amount of from about 0.1 mg / ml to about 1.0 mg / ml in broth medium, and from about 0.01 mg / ml to 1.0 mg / ml in agar medium.

The invention relates to an anaerobic reaction processing device and method for 1,4-butanediol chemical wastewater. The anaerobic reaction device internally comprises an inlet water distributor at the bottom of the device, an anaerobic sludge bed in the middle of the device and a three-phase separator at the top of the device, which are communicated with one another in sequence, wherein the anaerobic sludge bed consists of s sludge bed body formed by a great amount of anaerobic microorganisms; the anaerobic reaction device is sequentially connected with a sludge outlet pipe, a water outlet pipe and a methane outlet pipe from bottom to top; the inlet water distributor is connected with a wastewater inlet pipe; and the water outlet pipe is provided with an outlet water return branch connected with the wastewater inlet pipe. The anaerobic reaction processing device can be used for processing the wastewater to obtain biomass energy methane. After being processed by the anaerobic reaction processing device and method, the high-concentration production wastewater can meet the requirements on discharge of nanotubes, or the difficulty and cost of following aerobic treatment can be reduced. The method is stable in operation, is high in efficiency, can ensure the stable quality of outlet water, can be used for facilitating automatic control, management and operation, and is low in operation cost.

The invention discloses a method for strengthening bagasse fermentation to produce hydrogen by using calcium carbonate and application. The method comprises the following steps: adding calcium carbonate in a basal fermentation culture medium to obtain a final fermentation culture medium; inoculating thermophilic anaerobic clostridium to perform anaerobic fermentation to obtain hydrogen. The bagasse in the method is common waste, and is in favor of directly degrading and utilizing the thermophilic anaerobic clostridium due to large specific surface area; the operation of adding a few of calcium carbonate on the basal fermentation culture medium is in favor of the utilization of the thermophilic anaerobic microorganism to the bagasse, and the hydrogen production capacity is greatly improved. Therefore, the method is especially suitable for preparing hydrogen.

Aiming at the problems of polyethylene terephthalate (PET) plastic degradation in the prior art, the invention provides a novel PET degradation biocatalyst and a construction method thereof. The PET degradation whole-bacterium catalyst is obtained by expressing PET enzyme in a heat-resistant strain; the PET enzyme has a sequence of SEQ NO.1 or a sequence with similarity of 99% with the sequence ofSEQ NO.1; and the heat-resistant strain is clostridium thermofibrillae. The construction method of the PET degradation whole-bacterium catalyst comprises the following steps: (1) expressing PET degradation enzyme by a plasmid; (2) expressing PET degradation enzyme by a genome; and (3) expressing PET degradation bodies. The PET degradation whole-bacterium catalyst overcomes the problem of feedbackinhibition, not only has degradation efficiency obviously higher than a known whole-bacterium biodegradation system, but also has a culture condition free of aeration and stirring as an anaerobic microorganism, so that process cost is obviously reduced. In addition, simultaneous degradation of fibers and PET in a blended fabric is realized through a one-pot method; the reaction temperature is low, early-stage separation of fibers is not needed, and an additional carbon source is not needed in a degradation process, so that the PET degradation whole-bacterium catalyst has remarkable economy and efficiency.

The invention relates to a method for screening natural gas biological desulphurization flora, which comprises the following steps: respectively collecting soil polluted by sulphur near a sulphur recovery device in a natural gas purifying factory and active mud at a sewage treatment stage; placing a sample in an inorganic nutrient solution for anaerobic microbe enrichment culture, wherein the culture temperature is 35 DEG C; performing 3-stage induction domestication liquid culture on an enriched nutrient solution; taking NaHS concentration and pressure as an induction domestication factor, and performing directed screening to obtain the microbe flora which can satisfy the natural gas biological desulphurization requirement. The method for screening natural gas biological desulphurization flora can greatly accelerate the growth speed of desulfurated microbe; compared with a traditional screening method, the method for screening natural gas biological desulphurization flora has the advantages of simple operation flow, clear screening target and high screening efficiency.

The embodiment of the invention discloses a sewage treatment method and a sewage treatment system of a combined dynamic membrane bioreactor, and is used for maintaining high-efficiency activated sludge and relatively high nitrification efficiency and improving the probability of pollutant removal. The method comprises the following steps: combining a regulating tank with an anaerobic tank throughan anaerobic regulating tank, and decomposing organic polymer pollutants in sewage flowing in through a grid into organic low-molecular pollutants by utilizing the degradation capacity of facultativeanaerobic microorganisms and anaerobic microorganisms so as to obtain first sewage containing the organic low-molecular pollutants; decomposing the first sewage containing organic low-molecular pollutants into carbon dioxide and water by utilizing the decomposition capacity of aerobic microorganisms through a biological membrane reaction tank to obtain treated second sewage; intercepting the treated second sewage through a biological membrane separation tank by utilizing an improved polyvinylidene fluoride (PVDF) membrane and a self-generated dynamic membrane to obtain treated third sewage, enabling intercepted sludge to flow back to the anaerobic regulation tank, and removing nitrogen by utilizing a denitrification effect.

The invention discloses a preparation method of a microbial coupling catalytic system for efficient catalytic conversion of CO2, which comprises the following steps: drying and dewatering a precursorof an iron-based active metal catalyst to obtain a solid A; dissolving an auxiliary agent salt into a solvent to obtain a solution B; dipping the solution B into the solid A powder, stirring, and drying to evaporate water to obtain a solid C; tabletting the solid C to 20-40 meshes, and activating to obtain a solid D; grinding the solid D to obtain a catalyst E; and mechanically and physically mixing the catalyst E and the domesticated anaerobic microorganisms by using a shaking table under an anaerobic condition to obtain the microorganism coupling catalysis system for efficiently catalyzing and converting CO2. According to the preparation method, anaerobic microorganisms and the iron-based catalyst are coupled to obtain a catalytic system, and the anaerobic microorganisms can be accelerated to efficiently catalyze and convert inert CO2 into high-added-value products such as methanol, formic acid or acetic acid, so that the efficient utilization of CO2 is realized, and the efficient output of the high-added-value products is realized.

The invention provides an anaerobic microorganism culture and real-time observation device. The device comprises a base, an upper cover, a micro-fluidic chip and a culture solution storage chamber; the base and the upper cover are mutually sealed and buckled, a first groove is used for accommodating the culture solution storage chamber, and a second groove is used for providing a culture space for anaerobic microorganisms; a third groove is used for accommodating a micro-fluidic chip, and a first window is formed in the horizontal groove surface of the third groove; a fourth groove is arranged opposite to the second groove, and a fifth groove is arranged opposite to the first groove; a second window is formed in the horizontal groove surface of the fourth groove; the first window and the second window are oppositely arranged; and a liquid outlet hole is formed in the culture solution storage chamber, and a gas inlet, a gas outlet and a liquid outlet are formed in the side wall of the upper cover. The device possesses excellent air tightness and can provide a stable anaerobic gas atmosphere for long-term stable liquid culture of anaerobic microorganisms, and the gas atmosphere is controllable; and the experimental requirements of high throughput, large-range multi-site sampling, high-magnification shooting and the like are met.

The invention provides an underflow-like constructed wetland type water treating method. According to the water treating method, water flows into a reaction cavity through water distribution holes, carbon source portions release organic matters into the water after being immerged into the water, oxygen content of the water in a lower cavity body is not larger than 2mg / L, the ratio of the total concentration of the organic matters in the water in the lower cavity body to the total concentration of Kjeldahl nitrogen in the water outside the reaction cavity is larger than 4, and accordingly, in the lower cavity body, anaerobic microorganism films are formed on biological stuffing units outside the carbon source portions, and the water is subjected to denitrification reaction on the anaerobic microorganisms; the water after being denitrified flows into an upper cavity body through flow guiding holes in flow guiding pieces; gas releasing portions in the upper cavity body release dissolved oxygen into the water, the oxygen content of the water in the upper cavity body is not smaller than 6mg / L, and accordingly, aerobic microorganism films are formed on the biological stuffing units outside the gas releasing portions, and the water on the aerobic microorganism films is subjected to aerobic reaction. The underflow-like constructed wetland type water treating method used for water purification is convenient to operate and high in water purification efficiency.

The invention discloses a method for enriching and separating perchloroethylene (PCE) dechlorinating bacteria and application. The invention realizes efficient enrichment culture of PCE complete dechlorinating bacteria, PCE can be completely dechlorinated after 26 days by using the dechlorinating mixed bacteria obtained by the enrichment method, the dechlorinating mixed bacteria show excellent dechlorinating effects, and the abundance of Dehalococoides in the dechlorinating mixed bacteria reaches 68.87%; and efficient PCE dechlorinating bacteria Geobacter is obtained by separation and purification from the enriched dechlorinating mixed bacteria by further using an ecology principle. The method provides favorable technical guidance for realizing efficient enrichment and separation of halogenated organic matter dehalogenation functional microorganisms and obtaining engineering bacterial agents, and has important application value in the technical field of halogenated organic matter anaerobic microorganism remediation.

The invention discloses a printing and dyeing wastewater efficient detoxification gradient treatment method based on an intelligent SBR electrode process, wherein efficient open-loop detoxification ofazo dye molecules in printing and dyeing wastewater is achieved through the action of a biological electrode, gradient concentration circulation treatment of the printing and dyeing wastewater in anSBR reactor is achieved through a circulation batch water inlet and outlet mode, and real-time prediction and accurate regulation and control of the SBR reactor are carried out by combining a neural network model to realize intelligent operation. According to the invention, the biological electrode effect, the multi-stage gradient concentration circulation effect of the reactor and the intelligenteffect of the neural network model are combined, so that azo dyes in printing and dyeing wastewater are subjected to intelligent and accelerated detoxification under the efficient effect of anaerobicmicroorganisms, and hydrolysis efficiency optimization and treatment capacity maximization are realized.

The invention discloses a method for restoring heavy metals by regulating microorganisms through iron-containing minerals, and belongs to the field of biological treatment of pollutants. The method comprises the steps of enriching and domesticating original florae in heavy metal contaminated soil and water, and then adding the enriched original florae and different iron-containing minerals into the heavy metal wastewater together. The iron-containing minerals have certain conductivity as a (semi)conductor material, and can be used as an electron shuttle carrier to mediate an electron transferprocess. Interspecific electron transfer among anaerobic microorganisms can be enhanced, directional regulation of communities are realized, and reduction of high-valence heavy metals can be cooperated. The required iron-containing minerals widely exist in the nature, the raw materials are easy to obtain, the operation is simple, the reduction activity of anaerobic microorganisms can be remarkablyregulated and controlled, and the remediation effect of heavy metal pollutants is enhanced.

The invention discloses a method for producing single-cell protein by microbial fermentation, which comprises the following steps: introducing mixed gas into a reactor filled with a domestication culture medium to obtain a domestication system for culturing and domesticating inoculum; introducing a mixed gas into the reactor filled with the fermentation medium to obtain a mixed fermentation system for fermentation culture of the domesticated microorganisms; inoculating anaerobic microorganisms and aerobic microorganisms into the domestication system for culture and domestication to acquire domesticated microorganisms; inoculating the domesticated microorganisms into a mixed fermentation system, and carrying out fermentation culture; and treating the fermentation product to obtain the single-cell protein. Compared with a single-substrate fermentation system, the double-substrate fermentation system for simultaneously supplying the mixed gas and the organic carbon source is more beneficial to microbial conversion of ammonia nitrogen and production of single-cell protein.

The invention discloses a method for treating lignite upgrading wastewater by anaerobic co-metabolism, relating to a method for treating coal chemical wastewater. The method solves the problems that the start is difficult, the operation is instable, the anaerobic microbial biomass is relatively low and does not achieve the expected effect and the like when the lignite upgrading wastewater is treated by adopting an anaerobic process. The method comprises the following main steps: selecting the lignite upgrading wastewater; selecting trehalose as a first co-metabolism matrix, wherein the ratio of the total COD (chemical oxygen demand) quantity of the trehalose added every day to the total COD quantity of the wastewater to be treated is controlled to be 0.75-1.5; starting an anaerobic reactor by adopting an inoculation cultivation method, wherein the adding quantity of the lignite upgrading wastewater is increased by 10% every week; keeping the hydraulic retention time for 12-24h; and controlling the stable working condition. The trehalose is used as the first matrix, so that organic matters which cannot serve as direct anaerobic microbial matrixes can be degraded, or the trehalose can strengthen the growth and propagation of anaerobic microbes using toxic and harmful matters as matrixes. After stable operation, the COD removal rate of the anaerobic treated lignite upgrading wastewater can reach 40-50%, and the removal rate of total phenols can reach 60-70%.

The invention discloses a method for treating wastewater through coupling of iron-carbon micro-electrolysis and anaerobic organisms, and relates to the technical field of environment-friendly wastewater treatment, and the method specifically comprises the following steps: inoculating or culturing anaerobic sludge in a wastewater treatment anaerobic bioreactor, operating the reactor, adding iron powder and carbon powder, continuously feeding and stirring wastewater, by an effluent precipitator, separating the wastewater from the coupled sludge, and returning the coupled sludge flows back intothe reactor through a reflux pump. According to the method provided by the invention, iron-carbon micro-electrolysis and anaerobic organisms are integrated in one reaction process, and meanwhile, theeffects of the iron-carbon micro-electrolysis and the anaerobic organisms on pretreatment of refractory or macromolecular organic pollutants in the wastewater are exerted. Through organic combinationof the two, on one hand, the anaerobic biological reaction provides an acidic condition for the iron-carbon micro-electrolysis reaction, so that the iron-carbon reaction is promoted, on the other hand, the iron powder / carbon powder is combined with the anaerobic biofloc to form a combination body with higher density, so that the settling performance of the anaerobic sludge can be enhanced, the enrichment of anaerobic microorganisms is facilitated, and the anaerobic biochemical effect is enhanced.

The invention discloses a method for synergistically treating antibiotic production wastewater, the method comprises the following steps: sequentially carrying out hydrolysis treatment, coagulation treatment, fluidized bed treatment and anaerobic biological treatment on the antibiotic production wastewater, and particularly, synergistically treating the antibiotic wastewater by adopting enhanced hydrolysis treatment, coagulation treatment, fluidized bed treatment and an up-flow anaerobic sludge bed reactor, and selectively destroying antibiotics in the wastewater before the wastewater enters an anaerobic biochemical system so that the antibiotic pressure of anaerobic microorganisms is relieved, and efficient anaerobic removal of COD in the wastewater is realized. Besides, screening and enrichment of antibiotics on drug-resistant bacteria and drug-resistant genes are blocked, so that control on the drug-resistant genes in the high-antibiotic wastewater treatment process is realized. The invention provides a combined process which is easy to operate, relatively low in cost, high in removal rate and good in stability aiming at the treatment of actual fermentation pharmaceutical wastewater, has wide adaptability and is suitable for popularization and application.

The invention belongs to the field of a biological technology, and relates to an application of eurotium chevalieri to fermented pit mud microorganism rejuvenation in a Baijiu fermentation pit and a method. Yellow fermenting liquor and last distillate which are generated in the solid-state fermentation of Baijiu are used as main fermentation substrates. The method comprises the steps of adding sterilized softened water, Daqu containing eurotium chevalieri and esterified red yeast rice, performing uniform stirring, inflating air, performing fermentation at 30-35 DEG C for 25-45 days, enabling the obtained fermentation liquid containing the eurotium chevalieri to be led to fermented pit mud in the fermentation pit or unstrained spirits, and performing fermentation, so as to increase the number of anaerobic microorganisms in the fermented pit mud, and effectively realize rejuvenation of microorganisms in the fermented pit mud in the fermentation pit. Besides, the application can also improve the flavor of the fermented pit mud in the fermentation pit, improve the fermentation environment of the unstrained spirits, can further improve the quality of strong fragrance Baijiu of Guojing group, and can improve the flavor of the Baijiu. The Guo brewed fragrant Baijiu having tea fragrance and fruit fragrance can be brewed.

The invention discloses a buried biochemical sewage treatment method, which comprises the following steps of: S1, solid-liquid separation: sequentially and obliquely arranging a plurality of layers offilter screens from top to bottom to perform solid-liquid separation on sewage, arranging a solid recovery tank at the lower ends of the filter screens, adjusting the pH value of the sewage to be neutral, and pumping the sewage into an anaerobic tank. Multi-layer solid-liquid separation is carried out on sewage; solid impurities in the sewage are reduced to the maximum extent; high-concentrationorganic wastewater is treated by anaerobic microorganisms, then organic substances are treated by aerobic microorganisms, residual organic matters are treated by an air flotation method, inorganic impurities are absorbed by sandstone, straws and silt, and residual water is subjected to emergent aquatic plant absorption treatment and biological diversification treatment, so that the treatment efficiency is improved, and the sewage residual degree is low.

The invention relates to a physical-chemical-biological combined treatment device and method for acid wastewater. The treatment device comprises a regulation and storage tank, a nitrogen deoxidization tank, an anaerobic alkaline slow-release neutralization and sedimentation tank, a drop aeration section, a sloping plank sedimentation tank, an anaerobic ecological wetland and a drying tank which communicate with each other in sequence. According to the treatment method, by reasonably controlling the dissolved oxygen content, the water passing mode and the physical primary sludge process of the acid wastewater at different stages, neutralization precipitation, physical sludge removal and long-acting microbial operation of the whole system are realized; long-acting slow release of an alkaline material and stable growth of anaerobic microorganisms are realized, and stable and efficient removal of heavy metals and inorganic salts is ensured; low-power operation and maintenance of the whole system can be realized through a water passing mode; physical primary mud can ensure stable operation of the whole system; and the system has the advantages of simple process, convenience in maintenance, low treatment cost and small occupied area, and the treated wastewater reaches the discharge standard for class-3 water bodies of surface water.

The invention discloses a pure culture method of anaerobic microorganisms. The pure culture method comprises the following steps of: step 1, preparing a liquid culture solution and boiling; dropwise adding resazurin; sub-packaging into a thin-opening pipe to obtain a culture pipe; step 2, introducing N2 / H2 mixed gas and aerating; rapidly covering a rubber plug tightly; step 3, putting the culture pipe into a high-pressure sterilization pot and sterilizing at high temperature; transferring into a super-clean platform and carrying out ultraviolet sterilization; and step 4, injecting a magnetic antioxidant into the culture pipe by utilizing an injector and uniformly mixing; injecting an anaerobic microorganism seed solution into the culture pipe by utilizing the injector; putting the culture pipe into a constant-temperature culture box and culturing to obtain an anaerobic microorganism group. The pure culture method of the anaerobic microorganisms has the beneficial effects that (1) an anaerobic culture box does not need to be utilized and the operation is simple and convenient; and (2) the magnetic antioxidant is not dissolved into the liquid culture solution and is not easily absorbed by the anaerobic microorganisms, so that the growth is not influenced; the separation of the anaerobic microorganisms can be simplified through magnetic separation and the relatively pure anaerobic microorganism group is obtained.

The invention discloses a UBF anaerobic reactor based on a biochar carrier. The UBF anaerobic reactor comprises a reaction tank, a biological filler layer is arranged on the upper portion of the reaction tank, a water inlet pipe is arranged on one side of the reaction tank, a sludge discharge pipe is arranged on the other side of the reaction tank, and the sludge discharge pipe, a water distributor, a granular sludge layer, the biological filler layer, an effluent weir and a gas collection port are sequentially arranged on the reaction tank from bottom to top. The anaerobic digestion capability of anaerobic microorganisms on sewage is improved through the biochar, and the anaerobic microorganisms in the reaction tank mainly exist in a biological membrane on the surface of a filter material through the biological filler layer, so that the quantity and activity of the microorganisms in the reaction tank are improved; according to the invention, the sewage treatment capability, the pollutant load impact resistance and the stability of a traditional UBF anaerobic reactor are improved, and the UBF anaerobic reactor has the characteristics of simplicity in operation, high treatment efficiency and the like.