135 results about "Anaeromicrobium sediminis" patented technology

A media supplement for culturing anaerobic bacteria is provided which comprises a filtrate of effluent from a chemostat vessel in which a target bacterial ecosystem has been cultured. Methods of using the supplement for culturing or isolating anaerobic microbial strains or communities, particularly anaerobic bacteria from the human gut, are also provided.

A method of producing in a bioreactor a biogas rich in methane involves electrolyzing water in an aqueous medium at a voltage in a range of from 1.8 V to 12 V in the presence of electrochemically active anaerobic microorganisms that biocatalyze production of hydrogen gas, and, contacting a species of hydrogenotrophic methanogenic microorganisms with the hydrogen gas and carbon dioxide to produce methane. Volumetric power consumption is in a range of from 0.03 Wh/L_R to 0.3 Wh/L_R. Current density is 0.01 A/cm_E² or lower. The voltage is sufficient to electrolyze water without destroying microbial growth. Such a method results in improved electrolysis efficiency while avoiding the use of noble metal catalysts. Further, a combination of water electrolysis with anaerobic degradation of organic matter results in increased biogas quality and in increased biogas quantity and yield. Oxidation of hydrogen sulfide contributes to the increased quality, while an increase in the rate of organic matter hydrolysis and an increase in the production of methane from hydrogen contributes to the increased quantity and yield.

The invention relates to a method for producing a clean energy source by a continuous feeding type anaerobic fermentation method, particularly to a continuous feeding type anaerobic fermentation method for preparing methane by fermenting catering organic waste. The method for producing the clean energy source through continuous feeding type anaerobic fermentation can be widely used for preparing the methane which is a clean energy source through the anaerobic fermentation of the catering organic waste, straws, animal excreta in farms and other organic waste. The method comprises the following steps of: draining partial moisture of the collected catering organic waste to enable the water content thereof to be not more than 80 percent, and then adding the processed catering organic waste and acclimated anaerobic activated sludge in a definite proportion into a reactor for fermentation. The catering organic waste is added into the reactor by adopting a continuous feeding method in the fermentation process, and a fermentation raw material is added once at regular intervals. An addition period is a gas production vigorous period. The addition is 0.1-10 percent of the volume of the reactor. An anaerobic state is strictly controlled in the whole process so as to facilitate the growth of anaerobes.

The invention discloses an upflow blanket filter (UBF) anaerobic reactor with a filter layer on the top and for treating chemical wastewater. The UBF anaerobic reactor comprises a reactor, a water distribution device, a sludge expanded bed, a three-phase separator, an effluent collecting device and a methane circulating device and is characterized in that the three-phase separator is arranged in the middle of the reactor; the sludge expanded bed is arranged at the lower part of the three-phase separator; a biological filling region is arranged at the upper part of the three-phase separator; the biological filling region comprises filling and a biological membrane; and the biological membrane is formed by attaching anaerobic microorganisms to the surface of the filling. The UBF anaerobic reactor is suitable for treating the chemical wastewater which has high salt content and low suspended matter content and is difficult to degrade; and gas lift internal circulation is adopted, namely methane generated in the reactor is collected by the three-phase separator and internal circulation is realized by using the methane as the lifting powder and without external mechanical power.

The invention relates to a wastewater treatment process with an effect of coupling nano magnetite for enhancing the activity of anaerobic microorganisms, and aims at solving the problems that a traditional anaerobic biological treatment system is easy to acidify, instable in performance and the like. Nano magnetite of which the average particle size is smaller than 100nm is prepared by the steps of dissolving ferric salts, precipitating, separating, washing and the like; the nano magnetite and anaerobic sludge are sufficiently and uniformly mixed at a proportion of 1.55gFe/g MLVSS (Mixed Liquor Volatile Suspended Solid) by a stirring method, a nitrogen blowing method and the like; a mixture is cultured by an anaerobic organism treatment device under the constant temperature condition of 35DEG C; with good electrical conductivity and high specific surface area of the nano magnetite, interspecific electron transfer between the anaerobic microorganisms is remarkably enhanced, the activity of the anaerobic microorganisms is greatly improved, and finally, the performance of an anaerobic organism treatment process is improved.

The invention relates to an anaerobic reaction processing device and method for 1,4-butanediol chemical wastewater. The anaerobic reaction device internally comprises an inlet water distributor at the bottom of the device, an anaerobic sludge bed in the middle of the device and a three-phase separator at the top of the device, which are communicated with one another in sequence, wherein the anaerobic sludge bed consists of s sludge bed body formed by a great amount of anaerobic microorganisms; the anaerobic reaction device is sequentially connected with a sludge outlet pipe, a water outlet pipe and a methane outlet pipe from bottom to top; the inlet water distributor is connected with a wastewater inlet pipe; and the water outlet pipe is provided with an outlet water return branch connected with the wastewater inlet pipe. The anaerobic reaction processing device can be used for processing the wastewater to obtain biomass energy methane. After being processed by the anaerobic reaction processing device and method, the high-concentration production wastewater can meet the requirements on discharge of nanotubes, or the difficulty and cost of following aerobic treatment can be reduced. The method is stable in operation, is high in efficiency, can ensure the stable quality of outlet water, can be used for facilitating automatic control, management and operation, and is low in operation cost.

The invention discloses a preparation method of a microbial coupling catalytic system for efficient catalytic conversion of CO2, which comprises the following steps: drying and dewatering a precursorof an iron-based active metal catalyst to obtain a solid A; dissolving an auxiliary agent salt into a solvent to obtain a solution B; dipping the solution B into the solid A powder, stirring, and drying to evaporate water to obtain a solid C; tabletting the solid C to 20-40 meshes, and activating to obtain a solid D; grinding the solid D to obtain a catalyst E; and mechanically and physically mixing the catalyst E and the domesticated anaerobic microorganisms by using a shaking table under an anaerobic condition to obtain the microorganism coupling catalysis system for efficiently catalyzing and converting CO2. According to the preparation method, anaerobic microorganisms and the iron-based catalyst are coupled to obtain a catalytic system, and the anaerobic microorganisms can be accelerated to efficiently catalyze and convert inert CO2 into high-added-value products such as methanol, formic acid or acetic acid, so that the efficient utilization of CO2 is realized, and the efficient output of the high-added-value products is realized.

The invention provides an anaerobic microorganism culture and real-time observation device. The device comprises a base, an upper cover, a micro-fluidic chip and a culture solution storage chamber; the base and the upper cover are mutually sealed and buckled, a first groove is used for accommodating the culture solution storage chamber, and a second groove is used for providing a culture space for anaerobic microorganisms; a third groove is used for accommodating a micro-fluidic chip, and a first window is formed in the horizontal groove surface of the third groove; a fourth groove is arranged opposite to the second groove, and a fifth groove is arranged opposite to the first groove; a second window is formed in the horizontal groove surface of the fourth groove; the first window and the second window are oppositely arranged; and a liquid outlet hole is formed in the culture solution storage chamber, and a gas inlet, a gas outlet and a liquid outlet are formed in the side wall of the upper cover. The device possesses excellent air tightness and can provide a stable anaerobic gas atmosphere for long-term stable liquid culture of anaerobic microorganisms, and the gas atmosphere is controllable; and the experimental requirements of high throughput, large-range multi-site sampling, high-magnification shooting and the like are met.

The invention discloses a method for enriching and separating perchloroethylene (PCE) dechlorinating bacteria and application. The invention realizes efficient enrichment culture of PCE complete dechlorinating bacteria, PCE can be completely dechlorinated after 26 days by using the dechlorinating mixed bacteria obtained by the enrichment method, the dechlorinating mixed bacteria show excellent dechlorinating effects, and the abundance of Dehalococoides in the dechlorinating mixed bacteria reaches 68.87%; and efficient PCE dechlorinating bacteria Geobacter is obtained by separation and purification from the enriched dechlorinating mixed bacteria by further using an ecology principle. The method provides favorable technical guidance for realizing efficient enrichment and separation of halogenated organic matter dehalogenation functional microorganisms and obtaining engineering bacterial agents, and has important application value in the technical field of halogenated organic matter anaerobic microorganism remediation.

The invention discloses a method for restoring heavy metals by regulating microorganisms through iron-containing minerals, and belongs to the field of biological treatment of pollutants. The method comprises the steps of enriching and domesticating original florae in heavy metal contaminated soil and water, and then adding the enriched original florae and different iron-containing minerals into the heavy metal wastewater together. The iron-containing minerals have certain conductivity as a (semi)conductor material, and can be used as an electron shuttle carrier to mediate an electron transferprocess. Interspecific electron transfer among anaerobic microorganisms can be enhanced, directional regulation of communities are realized, and reduction of high-valence heavy metals can be cooperated. The required iron-containing minerals widely exist in the nature, the raw materials are easy to obtain, the operation is simple, the reduction activity of anaerobic microorganisms can be remarkablyregulated and controlled, and the remediation effect of heavy metal pollutants is enhanced.

The invention discloses a method for producing single-cell protein by microbial fermentation, which comprises the following steps: introducing mixed gas into a reactor filled with a domestication culture medium to obtain a domestication system for culturing and domesticating inoculum; introducing a mixed gas into the reactor filled with the fermentation medium to obtain a mixed fermentation system for fermentation culture of the domesticated microorganisms; inoculating anaerobic microorganisms and aerobic microorganisms into the domestication system for culture and domestication to acquire domesticated microorganisms; inoculating the domesticated microorganisms into a mixed fermentation system, and carrying out fermentation culture; and treating the fermentation product to obtain the single-cell protein. Compared with a single-substrate fermentation system, the double-substrate fermentation system for simultaneously supplying the mixed gas and the organic carbon source is more beneficial to microbial conversion of ammonia nitrogen and production of single-cell protein.

The invention discloses a method for treating lignite upgrading wastewater by anaerobic co-metabolism, relating to a method for treating coal chemical wastewater. The method solves the problems that the start is difficult, the operation is instable, the anaerobic microbial biomass is relatively low and does not achieve the expected effect and the like when the lignite upgrading wastewater is treated by adopting an anaerobic process. The method comprises the following main steps: selecting the lignite upgrading wastewater; selecting trehalose as a first co-metabolism matrix, wherein the ratio of the total COD (chemical oxygen demand) quantity of the trehalose added every day to the total COD quantity of the wastewater to be treated is controlled to be 0.75-1.5; starting an anaerobic reactor by adopting an inoculation cultivation method, wherein the adding quantity of the lignite upgrading wastewater is increased by 10% every week; keeping the hydraulic retention time for 12-24h; and controlling the stable working condition. The trehalose is used as the first matrix, so that organic matters which cannot serve as direct anaerobic microbial matrixes can be degraded, or the trehalose can strengthen the growth and propagation of anaerobic microbes using toxic and harmful matters as matrixes. After stable operation, the COD removal rate of the anaerobic treated lignite upgrading wastewater can reach 40-50%, and the removal rate of total phenols can reach 60-70%.

The invention discloses a method for treating wastewater through coupling of iron-carbon micro-electrolysis and anaerobic organisms, and relates to the technical field of environment-friendly wastewater treatment, and the method specifically comprises the following steps: inoculating or culturing anaerobic sludge in a wastewater treatment anaerobic bioreactor, operating the reactor, adding iron powder and carbon powder, continuously feeding and stirring wastewater, by an effluent precipitator, separating the wastewater from the coupled sludge, and returning the coupled sludge flows back intothe reactor through a reflux pump. According to the method provided by the invention, iron-carbon micro-electrolysis and anaerobic organisms are integrated in one reaction process, and meanwhile, theeffects of the iron-carbon micro-electrolysis and the anaerobic organisms on pretreatment of refractory or macromolecular organic pollutants in the wastewater are exerted. Through organic combinationof the two, on one hand, the anaerobic biological reaction provides an acidic condition for the iron-carbon micro-electrolysis reaction, so that the iron-carbon reaction is promoted, on the other hand, the iron powder/carbon powder is combined with the anaerobic biofloc to form a combination body with higher density, so that the settling performance of the anaerobic sludge can be enhanced, the enrichment of anaerobic microorganisms is facilitated, and the anaerobic biochemical effect is enhanced.

The invention discloses a method for synergistically treating antibiotic production wastewater, the method comprises the following steps: sequentially carrying out hydrolysis treatment, coagulation treatment, fluidized bed treatment and anaerobic biological treatment on the antibiotic production wastewater, and particularly, synergistically treating the antibiotic wastewater by adopting enhanced hydrolysis treatment, coagulation treatment, fluidized bed treatment and an up-flow anaerobic sludge bed reactor, and selectively destroying antibiotics in the wastewater before the wastewater enters an anaerobic biochemical system so that the antibiotic pressure of anaerobic microorganisms is relieved, and efficient anaerobic removal of COD in the wastewater is realized. Besides, screening and enrichment of antibiotics on drug-resistant bacteria and drug-resistant genes are blocked, so that control on the drug-resistant genes in the high-antibiotic wastewater treatment process is realized. The invention provides a combined process which is easy to operate, relatively low in cost, high in removal rate and good in stability aiming at the treatment of actual fermentation pharmaceutical wastewater, has wide adaptability and is suitable for popularization and application.

The invention belongs to the field of a biological technology, and relates to an application of eurotium chevalieri to fermented pit mud microorganism rejuvenation in a Baijiu fermentation pit and a method. Yellow fermenting liquor and last distillate which are generated in the solid-state fermentation of Baijiu are used as main fermentation substrates. The method comprises the steps of adding sterilized softened water, Daqu containing eurotium chevalieri and esterified red yeast rice, performing uniform stirring, inflating air, performing fermentation at 30-35 DEG C for 25-45 days, enabling the obtained fermentation liquid containing the eurotium chevalieri to be led to fermented pit mud in the fermentation pit or unstrained spirits, and performing fermentation, so as to increase the number of anaerobic microorganisms in the fermented pit mud, and effectively realize rejuvenation of microorganisms in the fermented pit mud in the fermentation pit. Besides, the application can also improve the flavor of the fermented pit mud in the fermentation pit, improve the fermentation environment of the unstrained spirits, can further improve the quality of strong fragrance Baijiu of Guojing group, and can improve the flavor of the Baijiu. The Guo brewed fragrant Baijiu having tea fragrance and fruit fragrance can be brewed.

The invention discloses a buried biochemical sewage treatment method, which comprises the following steps of: S1, solid-liquid separation: sequentially and obliquely arranging a plurality of layers offilter screens from top to bottom to perform solid-liquid separation on sewage, arranging a solid recovery tank at the lower ends of the filter screens, adjusting the pH value of the sewage to be neutral, and pumping the sewage into an anaerobic tank. Multi-layer solid-liquid separation is carried out on sewage; solid impurities in the sewage are reduced to the maximum extent; high-concentrationorganic wastewater is treated by anaerobic microorganisms, then organic substances are treated by aerobic microorganisms, residual organic matters are treated by an air flotation method, inorganic impurities are absorbed by sandstone, straws and silt, and residual water is subjected to emergent aquatic plant absorption treatment and biological diversification treatment, so that the treatment efficiency is improved, and the sewage residual degree is low.

The invention discloses a pure culture method of anaerobic microorganisms. The pure culture method comprises the following steps of: step 1, preparing a liquid culture solution and boiling; dropwise adding resazurin; sub-packaging into a thin-opening pipe to obtain a culture pipe; step 2, introducing N2/H2 mixed gas and aerating; rapidly covering a rubber plug tightly; step 3, putting the culture pipe into a high-pressure sterilization pot and sterilizing at high temperature; transferring into a super-clean platform and carrying out ultraviolet sterilization; and step 4, injecting a magnetic antioxidant into the culture pipe by utilizing an injector and uniformly mixing; injecting an anaerobic microorganism seed solution into the culture pipe by utilizing the injector; putting the culture pipe into a constant-temperature culture box and culturing to obtain an anaerobic microorganism group. The pure culture method of the anaerobic microorganisms has the beneficial effects that (1) an anaerobic culture box does not need to be utilized and the operation is simple and convenient; and (2) the magnetic antioxidant is not dissolved into the liquid culture solution and is not easily absorbed by the anaerobic microorganisms, so that the growth is not influenced; the separation of the anaerobic microorganisms can be simplified through magnetic separation and the relatively pure anaerobic microorganism group is obtained.

The invention discloses a UBF anaerobic reactor based on a biochar carrier. The UBF anaerobic reactor comprises a reaction tank, a biological filler layer is arranged on the upper portion of the reaction tank, a water inlet pipe is arranged on one side of the reaction tank, a sludge discharge pipe is arranged on the other side of the reaction tank, and the sludge discharge pipe, a water distributor, a granular sludge layer, the biological filler layer, an effluent weir and a gas collection port are sequentially arranged on the reaction tank from bottom to top. The anaerobic digestion capability of anaerobic microorganisms on sewage is improved through the biochar, and the anaerobic microorganisms in the reaction tank mainly exist in a biological membrane on the surface of a filter material through the biological filler layer, so that the quantity and activity of the microorganisms in the reaction tank are improved; according to the invention, the sewage treatment capability, the pollutant load impact resistance and the stability of a traditional UBF anaerobic reactor are improved, and the UBF anaerobic reactor has the characteristics of simplicity in operation, high treatment efficiency and the like.

The embodiments of the invention disclose a thermophilic anaerobic microbial strain 64-1 and an application thereof. The strain has a Latin name of Phaeospirillum sp., is collected in General Microorganisms Center of China Committee for Culture Collection of Microorganisms on August 6, 2019, has a collection number of CGMCC 1. 16798 and is collected at 3#, courtyard 1#, west Beichen road, Chaoyangdistrict, Beijing, China. A 16S rDNA gene of the strain 64-1 has a full-length sequence of 1453bps; and shown by carrying out an analysis according to a comparison result of GenBank, a 16S rDNA genesequence of the strain and a 16S rDNA gene sequence of Telmatospirillum siberiense 26-4b1(T) have the degree of similarity of 92.47%. The thermophilic facultative anaerobic strain 64-1 CGMCC 1. 16798disclosed by the embodiments of the invention has a very good degradation action on high pour point oil, the surface tension of fermentation broth can be lowered by a biological surfactant generated by the strain, and thus, the strain has very good emulsifying and dispersing capability to the high pour point oil. The strain and cultures thereof can be used for increasing the recovery ratio of thehigh pour point oil.

The invention relates to a process for producing biogas from fibrous substrate by anaerobic fermentation. The process is characterised in that

• a) the fibrous substrate is fed together with process liquid to a fermenter containing anaerobic microorganisms depending on the TSS content ascertained in this fermenter,
• b) the fibrous substrate is subjected to wet fermentation in this fermenter to produce biogas
• c) the output containing fermented fibrous substrate is drawn off from the fermenter and the TSS content in the fermenter is ascertained,
• d) the TSS content ascertained is compared with a fixed target range and
• e) depending on the result from d), step a) is repeated with adjusted amounts in order to comply with the target range for the TSS in the fermenter.

The invention discloses a catalytic system for producing medium-chain fatty acid or/and alcohol, and a preparation method and application thereof. The catalytic system comprises an inorganic solid catalyst and anaerobic microorganisms for producing medium-chain fatty acid or/and alcohol; the inorganic solid catalyst comprises a main catalyst and an auxiliary agent; and the main catalyst comprisesone or two of metal oxide and metal carbide. The inorganic solid catalyst comprises a main catalyst and an auxiliary agent, the synthesis process is simple, and an excessive impregnation method is adopted for loading; the inorganic solid catalyst disclosed by the invention comprises the main catalyst and the auxiliary agent, so that CO can be adsorbed and a formic acid or acetic acid intermediatecan be generated in a reaction system for producing medium-chain fatty acid or/and alcohol, and the aim of promoting microbial reaction to generate medium-chain fatty acid and/or alcohol is achieved;and the inorganic solid catalyst provided by the invention is long in particle life and good in mechanical stability.