Method for producing single-cell protein by microbial fermentation

A single-cell protein and microbial fermentation technology, applied in the methods of using microorganisms, biochemical equipment and methods, microorganisms, etc., can solve problems such as loss of active nitrogen, reduce the level of active nitrogen, achieve resource recovery, and simple operation.

Pending Publication Date: 2021-04-30
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, the huge loss is due to the defects of traditional planting methods; on the other hand, high-ammonia-nitrogen wastes will be generated in each process of the nitrogen cycle, and th...

Method used

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  • Method for producing single-cell protein by microbial fermentation
  • Method for producing single-cell protein by microbial fermentation
  • Method for producing single-cell protein by microbial fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] A method for acclimating microorganisms that can be used to produce single-cell proteins, the specific steps are as follows:

[0086] 1) configure the basal medium, add 15g / L agar after sterilization; the formula of the basal medium is to contain the following amount of substances in every liter of solution: potassium dihydrogen phosphate 2.3g, disodium hydrogen phosphate 2.9g, chloride Ammonium 1g, magnesium sulfate 0.5g, sodium carbonate 0.5g, calcium chloride 0.01g, ferric ammonium citrate 0.05g, boric acid 0.6mg, cobalt chloride 0.4mg, zinc sulfate 0.2mg, manganese chloride 0.06mg, sodium molybdate 0.06mg, nickel chloride 0.04mg, copper sulfate 0.02mg;

[0087] 2) inoculation of anaerobic microorganisms and aerobic microorganisms;

[0088] 3) Adjust the temperature and pH of the domestication system, and the volume ratio of the feed is H 2 :O 2 :CO 2 = 60:25:15 mixed gas, reacted in the shaker reactor;

[0089] 4) Supplement the mixed gas to the reaction system t...

Embodiment 2

[0097] A method for efficiently producing single-cell protein by microbial fermentation, the specific steps are as follows:

[0098] 1) Configure the basal medium, add NH after sterilization 4 Cl to the initial nitrogen content in the system is 1000mgN / L; The formula of described basal medium is to contain the following amount of material in every liter of solution: Potassium dihydrogen phosphate 2.3g, disodium hydrogen phosphate 2.9g, ammonium chloride 1g, sulfuric acid Magnesium 0.5g, sodium carbonate 0.5g, calcium chloride 0.01g, ferric ammonium citrate 0.05g, boric acid 0.6mg, cobalt chloride 0.4mg, zinc sulfate 0.2mg, manganese chloride 0.06mg, sodium molybdate 0.06mg, chlorine Nickel 0.04mg, copper sulfate 0.02mg;

[0099] 2) Add glucose to the medium, and the amount of glucose added is 8.25g / L;

[0100] 3) Regulate temperature and pH, feed volume ratio is H 2 :O 2 :CO 2 = 60: 25: 15 mixed gas, reacted in the shaker reactor;

[0101] 4) Supplement the mixed gas to ...

Embodiment 3

[0108] Repeat Example 2, the difference is only: in step 2), the added amount of glucose is 16.50g / L.

[0109] The amount of ammonia nitrogen conversion and the yield of single-cell protein of microorganisms in the fermentation system of this example are shown in Table 2. As can be seen from Table 2, the amount of ammonia nitrogen conversion and single-cell protein production in the fermentation system of this embodiment are higher than that of Example 2, and the crude protein content in the product is close to that of Example 2.

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Abstract

The invention discloses a method for producing single-cell protein by microbial fermentation, which comprises the following steps: introducing mixed gas into a reactor filled with a domestication culture medium to obtain a domestication system for culturing and domesticating inoculum; introducing a mixed gas into the reactor filled with the fermentation medium to obtain a mixed fermentation system for fermentation culture of the domesticated microorganisms; inoculating anaerobic microorganisms and aerobic microorganisms into the domestication system for culture and domestication to acquire domesticated microorganisms; inoculating the domesticated microorganisms into a mixed fermentation system, and carrying out fermentation culture; and treating the fermentation product to obtain the single-cell protein. Compared with a single-substrate fermentation system, the double-substrate fermentation system for simultaneously supplying the mixed gas and the organic carbon source is more beneficial to microbial conversion of ammonia nitrogen and production of single-cell protein.

Description

technical field [0001] The invention relates to a production method of single-cell protein. More specifically, it relates to a method for microbial fermentation to produce single-cell protein. Background technique [0002] The world population is expected to grow to 9-10 billion in 2050, and the demand for food will increase by 30%-60%. With the improvement of living standards, people will have more and more additional demands for high-protein foods. Nitrogen in nature is mainly in the form of N 2 Exists in the form of the existing nitrogen cycle, N 2 It is mainly converted into active nitrogen through lightning, microbial fixation, HaberBosch process, etc., and then the active nitrogen is further fixed by crop planting. Part of the nitrogen fixed by crop planting is processed into plant protein products needed by people; the other part is converted into animal protein through animal husbandry, and then animal protein undergoes a series of processing to finally become ani...

Claims

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Application Information

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IPC IPC(8): C12P21/00C12N1/00C12N1/36
CPCC12P21/00C12N1/00C12N1/36
Inventor 王雯牛子津杨紫怡
Owner BEIJING UNIV OF CHEM TECH
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