The invention discloses a stem cell culture system. The stem cell culture system comprises a mounting box, a pretreatment mechanism, a cell dispersing mechanism, an isolating and cleaning mechanism, aconvenient test tube picking and placing mechanism, a temperature control mechanism and a cell centrifugation mechanism, wherein the bottom end of the mounting box is fixedly connected with a mounting bottom plate, the cell centrifugation mechanism is arranged on one side face of the outside of the mounting box and comprises a centrifuge main body, and a sealing cover is hinged to one side of oneside face, where the centrifuge main body is placed, of the outside of the mounting box. According to the stem cell culture system, a lifting outer tube can be lifted from the top of a lifting circular tube through an offsetting spring, a top end plane of a connecting flip plate can restores horizontal, thus, a fixing ring and a top end plane of a placement tube can be horizontal, then, the placement tube can correspond to a test tube position of the convenient test tube picking and placing mechanism, thus, a test tube can be more easily placed inside the placement tube, and thus, a cell dispersing device and a stem cell centrifugation mechanism can be more conveniently used in a matched manner.