Method for screening natural gas biological desulphurization flora

A technology for biological desulfurization and screening methods, applied in biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of sulfide tolerance and pressure domestication not yet involved, easy to ignore growth target bacteria, and high work intensity. The effect of small workload, short cycle and accelerated growth rate

Active Publication Date: 2013-07-03
PETROCHINA CO LTD
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Problems solved by technology

Moreover, the traditional method is to verify the transformation ability of each strain of these small colonies one by one. This screening method is labor-intensive, has low screening efficiency, and is easy to ignore target bacteria that grow slowly.
At the same time, the current conventional screening methods do not address the special working conditions of natural gas biological desulfurization, and the specific measures and implementation rules for sulfide tolerance and pressure acclimation have not yet been involved.

Method used

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  • Method for screening natural gas biological desulphurization flora
  • Method for screening natural gas biological desulphurization flora

Examples

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Effect test

Embodiment 1

[0018] Screening of natural gas biological desulfurization bacteria in sulfur-contaminated soil:

[0019] Take the sulfur-contaminated soil (15-20cm) near the sulfur recovery unit of the natural gas purification plant, pass it through a 60-mesh sieve, add 5g of the sieved fine-grained soil sample into an anaerobic culture bottle filled with inorganic nutrient solution, and conduct anaerobic culture The bottle was placed on a constant temperature magnetic stirrer, and an external mixed gas source was connected. The culture temperature was 35°C, and the culture was stirred for 7 days. The composition of the inorganic nutrient solution includes carbon source, nitrogen source and buffer ion. The gas mixture composition is: 80% N 2 +20%CO 2 .

[0020] Add the culture solution into a sealed pressure-resistant container with an equal volume and a concentration of 0.04g / L NaHS, fill it with nitrogen to a pressure of 2MPa, and after standing still for 2 days, transfer it to a cultu...

Embodiment 2

[0025] Screening of desulfurization bacteria in activated sludge in sewage treatment section of natural gas purification plant:

[0026] Take the activated sludge from the sewage treatment section of the natural gas purification plant, centrifuge it at 10,000rpm for 15 minutes, and then take an appropriate amount of sediment and add it to an anaerobic culture bottle filled with inorganic nutrient solution. The anaerobic culture bottle is placed on a constant temperature magnetic stirrer and connected to a mixed gas source. The culture temperature is 35°C, and the stirring culture is 7

[0027] d. The composition of the inorganic nutrient solution is as follows: wherein the composition of the inorganic nutrient solution includes carbon source, nitrogen source and buffer ion. The gas mixture composition is: 90% N 2 +10%CO 2 .

[0028] Add the culture solution into a sealed pressure-resistant container with an equal volume and a concentration of 0.04g / L NaHS, fill it with nit...

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Abstract

The invention relates to a method for screening natural gas biological desulphurization flora, which comprises the following steps: respectively collecting soil polluted by sulphur near a sulphur recovery device in a natural gas purifying factory and active mud at a sewage treatment stage; placing a sample in an inorganic nutrient solution for anaerobic microbe enrichment culture, wherein the culture temperature is 35 DEG C; performing 3-stage induction domestication liquid culture on an enriched nutrient solution; taking NaHS concentration and pressure as an induction domestication factor, and performing directed screening to obtain the microbe flora which can satisfy the natural gas biological desulphurization requirement. The method for screening natural gas biological desulphurization flora can greatly accelerate the growth speed of desulfurated microbe; compared with a traditional screening method, the method for screening natural gas biological desulphurization flora has the advantages of simple operation flow, clear screening target and high screening efficiency.

Description

technical field [0001] The invention relates to the technical field of natural gas biological desulfurization, in particular to a rapid screening method for natural gas biological desulfurization strains. Background technique [0002] Among many natural gas processing technologies, biological desulfurization is a new means of natural gas purification. It can use the metabolic process of microorganisms to desulfurize under normal temperature and pressure. It does not require expensive treatment solutions and catalysts, nor does it generate a lot of waste. The operating conditions are mild, the process is simple, and the operating costs are low. The requirements of the natural gas industry for the desulfurization process. Judging from the research status, although the preliminary technology of biological desulfurization of natural gas has been formed abroad, it has not involved the screening method of natural gas desulfurization strains. [0003] The screening of desulfuriza...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00
Inventor 徐波黄黎明何金龙黄韵弘刘其松涂彦闫琛洋张伍
Owner PETROCHINA CO LTD
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