Method for restoring heavy metals by regulating microorganisms through iron-containing minerals

A technology for heavy metals and microorganisms, applied in the field of biological treatment of pollutants, can solve the problems of being easily affected by environmental factors and long reaction time, and achieve the effects of enhanced electron transfer, simple operation, and easy availability of raw materials.

Inactive Publication Date: 2021-01-01
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Microbial remediation technology does not need to destroy the soil structure, has high safety and low investment cost. It is an environmentally friendly technology, but its reaction time is long and it is easily affected by environmental factors, such as temperature, humidity, pH, etc. There is some resistance in

Method used

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  • Method for restoring heavy metals by regulating microorganisms through iron-containing minerals
  • Method for restoring heavy metals by regulating microorganisms through iron-containing minerals
  • Method for restoring heavy metals by regulating microorganisms through iron-containing minerals

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Weigh 1 g of moist soil and 10 mL of chromium solution into 100 mL of anaerobic medium, and culture at 200 rpm and 30ºC. When the microorganisms are cultivated to the logarithmic phase, the soil particles in the culture medium are centrifugally filtered. When transferring, first mix the bacterial solutions of each culture medium in equal amounts, and then transfer and enrich again according to the amount of 10%, and cultivate at a constant temperature until the culture liquid Cloudiness occurs.

[0031] According to the inoculum volume of 1%, take 1 mL of the culture medium and transfer it to 100 mL of fresh medium as the bacteria used for inoculation. In order to minimize the loss of microbial diversity, the medium used was a nutrient-rich environment (LB) medium, and the composition of the medium was: 10 g / L peptone, 5 g / L yeast extract, 10 g / L chloride NaCl, and adjust the pH to 7.0 with HCl and NaOH.

[0032] Using the anaerobic bottle as a reactor, add 0.1 g of m...

Embodiment 2

[0035] Using the anaerobic bottle as a reactor, add 0.1 g of hematite to 100 mL of culture solution, and add the enriched microbial flora at an inoculum of 1%. Potassium dichromate (K 2 Cr 2 o 7 ) mother liquor, organic matter sodium acetate (CH3COONa) was added to the medium to make the initial Cr(VI) concentration of the system reach 50mg / L, CH3COO - The concentration is 10 mmol / L. Wherein Cr(VI) is electron acceptor, CH3COO - as an electron donor. The culture conditions were 30°C, 200 rpm, and the experiment of microorganism-mineral synergistic reduction of Cr(VI) was carried out. Sampling to detect the concentration of Cr(VI)

[0036] In order to compare the promoting effect of the present invention, two groups of contrasts are set at the same time, wherein hematite is not added in the control group 1, and all the other parameters are the same as the experimental group; only hematite is added in the control group 2, and the microorganisms of domestication are not ino...

Embodiment 3

[0038] Using the anaerobic bottle as a reactor, add 0.1 g of pyrite to 100 mL of culture solution, and add the enriched microbial flora at an inoculum of 1%. Potassium dichromate (K 2 Cr 2 o 7 ) mother liquor, organic sodium acetate (CH 3 COONa) was added to the medium to make the initial Cr(VI) concentration of the system reach 50mg / L, CH 3 COO - The concentration is 10 mmol / L. Wherein Cr(VI) is electron acceptor, CH3COO -as an electron donor. The culture condition is 30°C, 200 rpm, and the experiment of microorganism-mineral synergistic reduction of Cr(VI) is carried out.

[0039] In order to compare the promotion effect of the present invention, two groups of contrasts are set at the same time, wherein no pyrite is added in the control group 1, and all the other parameters are the same as the experimental group; only pyrite is added in the control group 2, and the microorganisms of domestication are not inoculated, and other parameters Same as the experimental group...

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Abstract

The invention discloses a method for restoring heavy metals by regulating microorganisms through iron-containing minerals, and belongs to the field of biological treatment of pollutants. The method comprises the steps of enriching and domesticating original florae in heavy metal contaminated soil and water, and then adding the enriched original florae and different iron-containing minerals into the heavy metal wastewater together. The iron-containing minerals have certain conductivity as a (semi)conductor material, and can be used as an electron shuttle carrier to mediate an electron transferprocess. Interspecific electron transfer among anaerobic microorganisms can be enhanced, directional regulation of communities are realized, and reduction of high-valence heavy metals can be cooperated. The required iron-containing minerals widely exist in the nature, the raw materials are easy to obtain, the operation is simple, the reduction activity of anaerobic microorganisms can be remarkablyregulated and controlled, and the remediation effect of heavy metal pollutants is enhanced.

Description

technical field [0001] The invention relates to a restoration method for reducing heavy metals by microbes controlled by iron-containing minerals, and belongs to the field of biological treatment of pollutants. Background technique [0002] Microbial remediation technology does not need to destroy the soil structure, has high safety and low investment cost. It is an environmentally friendly technology, but its reaction time is long and it is easily affected by environmental factors, such as temperature, humidity, pH, etc. There is some resistance. But in general, microbial remediation is still a very promising technology. [0003] The energy metabolism process of extracellular respiration bacteria mainly relies on the oxidation of organic matter to generate electrons, and then transfers intracellular electrons to extracellular electron acceptors through the "breathing" chain, accompanied by energy generation. Based on this, extracellular respiration bacteria can oxidize or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/08B09C1/10C22B34/32C22B7/00
CPCB09C1/085B09C1/10C22B34/32C22B7/006Y02P10/20
Inventor 甘敏朱建裕章可陈芳陈耀宗何鹏刘梦飞
Owner CENT SOUTH UNIV
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