Pure culture method of anaerobic microorganisms

Abstract

The invention discloses a pure culture method of anaerobic microorganisms. The pure culture method comprises the following steps of: step 1, preparing a liquid culture solution and boiling; dropwise adding resazurin; sub-packaging into a thin-opening pipe to obtain a culture pipe; step 2, introducing N2 / H2 mixed gas and aerating; rapidly covering a rubber plug tightly; step 3, putting the culture pipe into a high-pressure sterilization pot and sterilizing at high temperature; transferring into a super-clean platform and carrying out ultraviolet sterilization; and step 4, injecting a magnetic antioxidant into the culture pipe by utilizing an injector and uniformly mixing; injecting an anaerobic microorganism seed solution into the culture pipe by utilizing the injector; putting the culture pipe into a constant-temperature culture box and culturing to obtain an anaerobic microorganism group. The pure culture method of the anaerobic microorganisms has the beneficial effects that (1) an anaerobic culture box does not need to be utilized and the operation is simple and convenient; and (2) the magnetic antioxidant is not dissolved into the liquid culture solution and is not easily absorbed by the anaerobic microorganisms, so that the growth is not influenced; the separation of the anaerobic microorganisms can be simplified through magnetic separation and the relatively pure anaerobic microorganism group is obtained.

Description

technical field

[0001] The invention relates to the technical field of bioengineering, in particular to a method for pure cultivation of anaerobic microorganisms. Background technique

[0002] In the common anaerobic microbial culture process, there are fermentation methods and multi-microbial co-culture methods, but there will be separation problems. In the pure culture method, anaerobic incubators or anaerobic culture tanks are generally used to cultivate anaerobic microorganisms, but this method is more complicated to operate and is more suitable for solid medium culture, not liquid medium culture. At present, reducing agents such as cysteine ​​and ascorbic acid are pre-added in common liquid culture media to reduce the oxidation-reduction potential and isolate oxygen, thereby forming an anaerobic environment for culturing microorganisms. However, in this method, the added reducing dose is too much, the solubility is large, and it is eaten by microorganisms, which affect...

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