6 results about "Geobacter" patented technology

The invention provides a complex microbial inoculant and application thereof in degradation of starch and biofuel cells, and belongs to the cross technical field of environmental biotechnology and bioelectrochemistry. According to the invention, a complex microbial inoculant composed of three microorganisms is constructed through a synthetic biological strategy; the complex microbial inoculant comprises genetically engineered Escherichia coli capable of rapidly converting starch into monosaccharide easy to use, shewanella for bearing upstream metabolites and geobacter for generating electricity, wherein the genetically engineered Escherichia coli is used for rapidly converting starch into monosaccharide easy to use; according to the invention, through precise function division of three strains, a complex degradation and power generation process is decomposed into a plurality of sub-steps catalyzed by special strains, so that an efficient'metabolic assembly line 'is formed, the inhibition of intermediate products is reduced, and the overall energy conversion efficiency is improved; the complex microbial inoculant can be used for degrading starch and preparing microbial fuel cells, realizes wastewater purification and recovery of bioelectric energy at the same time, and has remarkable environmental and economic benefits.

This invention belongs to the field of microbiology, specifically relating to a biofilm sensor and a method for detecting biotoxicity using it. The specific technical solution involves a bacterial preparation comprising *Geobacterium*, *Acinetobacter*, and *Pseudomonas*. A sensor is prepared using this bacterial preparation and used to detect the biotoxicity of pollutants. This invention provides a novel biofilm sensor that, based on the high electroactivity of *Geobacterium* and by limiting the amount of carbon source used during cultivation, ultimately achieves an electroactive biofilm with a thickness lower than that of existing technologies, and realizes extremely high detection sensitivity, possessing enormous potential for detection applications.

This application discloses an in-situ remediation method for uranium contamination and its application. The method involves the interaction between microorganisms and pyrite, driving pyrite hydrolysis, oxidation, atomic rearrangement, and biomineralization. This immobilizes the uranium contaminant within the iron oxide lattice of the reconstructed product, thus remediating the contaminated area. The microorganisms include one or more of Brucella, Geobacterium, or Clostridium. If the uranium-contaminated area contains microorganisms, natural humic substances are used as electron donors to stimulate the in-situ microorganisms. A natural humic substance solution is injected into the uranium-contaminated area via a circulating pumping method to stimulate the in-situ microorganisms. The concentration of the natural humic substance solution is 0.05–5 g / L, and the dosage is 5–500 g / day. This application fully considers and rationally utilizes in-situ mineral, microbial, and geochemical resources, relying on the in-situ mineral and microbial interactions to save engineering costs and achieve high remediation efficiency.

The invention discloses a method for promoting biological nitrogen fixation in a rice field in situ, which does not need to externally inoculate electroactive microorganisms or nitrogen-fixing microorganisms and does not apply an external power supply, and comprises the following steps: S1, carrying out acid treatment and / or hydrophilization treatment on a conductive material; s2, constructing a conductive interface: laying a conductive material on soil of a plowing layer of the rice field and enabling the conductive material to be in close contact with the soil so as to form the conductive interface, and performing waterflooding treatment on the plowing layer of the rice field on which the conductive material is laid; and S3, culturing under a waterflooding anaerobic condition to form a biological membrane on the surface of the conductive interface. According to the method, exogenous electroactive microbial agents or nitrogen-fixing microbial agents do not need to be added, in-situ microorganisms are induced to spontaneously adhere to the surface of the conductive material in the anaerobic environment of the rice field to form a film, community reconstruction is achieved, the relative abundance of electroactive microorganisms such as geobacteria and nitrogen-fixing microorganisms in the surface biological film is improved, the abundance of nitrogen-fixing related genes is increased, and the nitrogen-fixing effect is improved. The rice field biological nitrogen fixation effect is promoted.

This invention discloses a biofilm-forming microbial consortium with acrylonitrile degradation function, its preparation method, and its application. The microbial consortium consists of *Agrobacterium parakneissus*, *Burkholderia szechuanensis*, *Trichophyton spp.*, *Rhodococcus rubrum*, and *Mycobacterium tumefaciens*; the ratio of viable bacteria of *Burkholderia parakneissus*, *Burkholderia szechuanensis*, *Trichophyton spp.*, *Oligotrophomonas spp.*, and *Trichophyton spp.* is 3:1:1:1:1. This microbial consortium has a stable microbial community structure and can rapidly form a stable biofilm on the surface of biofilter packing material, exhibiting high acrylonitrile degradation efficiency. It is simple to operate, low in cost, and requires only a small amount of additional inorganic nutrients during treatment, without causing secondary pollution, making it an environmentally friendly technology. Its application prospects in the treatment of acrylonitrile-containing wastewater (gas) are broad.

The invention belongs to the technical field of microbial agents, and provides a methyl nutrition complex microbial inoculant and an application thereof in rice methane emission reduction, and the complex microbial inoculant comprises facultative methylobacterium, sphingobacterium, escherichia coli, variovorax, flavobacterium, sphingolipid, pseudoyellow microbacterium, Knoobrucella, sedimentary bacillus and terranean bacillus. The complex microbial inoculant disclosed by the invention can effectively reduce methane emission in rice planting, and has the functions of reducing emission, improving efficiency and promoting stable yield.