43 results about "Geobacter" patented technology

The invention relates to a sulfadimidine degrading bacterium S-07 degraded by microorganisms and a screening method thereof. The sulfadimidine degrading bacterium S-07 is verified as Geobacillus, and the Genbank registration number of the bacterium 16S rDNA is KU588289. The sulfadimidine degrading bacterium is preserved in the China General Microbiological Culture Collection Center on April 21st, 2016, and the preservation number of the sulfadimidine degrading bacterium is CGMCC No. 12382. The sulfadimidine degrading bacterium S-07 well grows within the temperature range of 50-75 DEG C, has quite strong tolerance to sulfadimidine, and can efficiently degrade sulfadimidine in a liquid phase and a solid phase. The bacterium can be used for degrading and removing sulfadimidine residues in livestock excrement or sludge high-temperature compost, the removal rate is larger than 95%, and the sulfadimidine degrading bacterium S-07 has good industrial application prospects and environment benefits.

In preferred embodiments, the present invention provides new isolated strains of a Geobacter species that are capable of using a carbon source that is selected from C₃ to C₁₂ organic compounds selected from pyruvate or metabolic precursors of pyruvate as an electron donor in metabolism and in subsequent energy production. In other aspects, other preferred embodiments of the present invention include methods of making such strains and methods of using such strains. In general, the wild type strain of the microorganisms has been shown to be unable to use these C₃ to C₁₂ organic compounds as electron donors in metabolic steps such as the reduction of metallic ions. The inventive strains of microorganisms are useful for improving bioremediation applications, including in situ bioremediation (including uranium bioremediation and halogenated solvent bioremediation), microbial fuel cells, power generation from small and large-scale waste facilities (e.g., biomass waste from dairy, agriculture, food processing, brewery, or vintner industries, etc.) using microbial fuel cells, and other applications of microbial fuel cells, including, but not limited to, improved electrical power supplies for environmental sensors, electronic devices, and electric vehicles.

The invention discloses a two-phase anaerobic treatment device and process for establishing direct interspecies electron transfer based on ethanol-type fermentation. The process comprises the following steps: respectively taking a fully mixed anaerobic digester and an up-flow anaerobic sludge bed reactor as an acidification phase and a methanogenesis phase; respectively controlling the hydraulic retention time of the acidification phase and the methanogenesis phase, and the pH and temperature in the device; regulating the fermentation pH of the acidification phase to be 4.0 to 4.5 by using 2 to 4mol/L HCI solution, and running the process. The two-phase anaerobic treatment device and process can achieve the following effects of improving the content of ethyl alcohol in an acidized product;improving the abundance of geobacter in granule sludge of the methanogenesis phase and promoting the establishment of direct interspecies electron transfer with methanogens; accelerating the decomposition rate of organic acid and alcohol substances in the methanogenesis phase; mitigating the acidity accumulation and syntrophism metabolism stasis of the methanogenesis phase caused by high organicloading; and improving the methanogenesis efficiency. The technology has the advantages of low investment cost, simple operation, remarkable improvement effect, and can be applied to the upgrading andrebuilding of the existing two-phase anaerobic process for improving the resistance to high load wastewater impact.

The invention discloses a method for enriching and separating perchloroethylene (PCE) dechlorinating bacteria and application. The invention realizes efficient enrichment culture of PCE complete dechlorinating bacteria, PCE can be completely dechlorinated after 26 days by using the dechlorinating mixed bacteria obtained by the enrichment method, the dechlorinating mixed bacteria show excellent dechlorinating effects, and the abundance of Dehalococoides in the dechlorinating mixed bacteria reaches 68.87%; and efficient PCE dechlorinating bacteria Geobacter is obtained by separation and purification from the enriched dechlorinating mixed bacteria by further using an ecology principle. The method provides favorable technical guidance for realizing efficient enrichment and separation of halogenated organic matter dehalogenation functional microorganisms and obtaining engineering bacterial agents, and has important application value in the technical field of halogenated organic matter anaerobic microorganism remediation.

The invention provides a method for synthesizing an organic matter by catalytically reducing CO2 through an MES biological cathode. A biological cathode is prepared in a microbial electrosynthesis system, CO2 is introduced into a cathode chamber for cyclic aeration, and the polarization potential range is set to be -0.6 V to -1.0 V (vs.Ag/AgCl). Microorganisms Acetobacterium, Candida sp. S and Geobacter which are domesticated and cultured on the cathode can directly obtain electrons from the electrode or hydrogen generated by the electrode to reduce CO2 and synthesize organic matters. The rateof synthesizing organic matters by reducing CO2 is high, and the method has important application value for CO2 fixed conversion and synthesis of organic chemicals.

Methods, microbial fuel cells and microbial consortia for generating electrical current are provided according to the present invention which include providing a microbial consortium to an anode chamber of a microbial fuel cell, wherein the microbial CN consortium includes: 1) an engineered methanogen that contains a heterologous nucleic acid sequence encoding methyl-coenzyme M reductase derived from an anaerobic methane oxidizer, 2) an exoelectrogen microbe that produces electrically-conductive appendages and/or one or more types of electron carrier, and 3) a sludge, methane-acclimated sludge, a sludge isolate component, a methane-acclimated sludge isolate component chosen from Paracoccus spp., Geotoga spp., Geobacter spp., Methanosarcina spp., Garciella spp., humic acids; or a combination of any two or more thereof.

The invention discloses a method for extracting geobacter extracellular polymeric substances. A microbial fuel cell is utilized to culture a biological film, ultrasound and centrifugation are combinedto extract the geobacter extracellular polymeric substances in a layered mode, and soluble S-EPS, loosely bound EPS (LB-EPS) and tightly bound EPS (TB-EPS) can be extracted out. Bacteria damage in anextracting process is effectively reduced, and the extracting amount of the extracellular polymeric substances is improved. According to the method, the characteristics of the geobacter extracellularpolymeric substances are represented for the first time, and the extracted geobacter extracellular polymeric substances is favorable for environmental pollution rehabilitation; furthermore, the method has a certain application prospect in extracellular electron transfer pathways and environment rehabilitation.

The invention provides a method for in-situ fluorescent labeling of geobacillus. The method includes transferring a PpFbFP fluorescent protein gene into geobacillus. The PpFbFP fluorescent protein label is not influenced by oxygen, can make the labeled geobacillus strain fluoresce under anaerobic conditions, and has a long half-life, and can realize long-term real-time observation of movement trajectory, film forming process and group behavior of the geobacillus.

The invention discloses a Geobacteraceae sulferreducens Geo-LYY strain for dehalogenation of halogenated organic compounds and dye decoloration and application of the Geobacteraceae sulferreducens Geo-LYY strain. According to the invention, a strain of Geobacteraceae sulferreducens LYY with halogenated organic compound dehalogenation and dye decolorization capabilities is obtained through screening and separation; the strain was preserved in Guangdong Microbial Culture Collection Center on February 17, 2020, and the preservation number of the strain is GDMCC No: 61003. The Geobacteraceae sulferreducens LYY can tolerate the pressure of environmental conditions of high-concentration tetrachloroethylene, antibiotics, heavy metal Cu<2+> and heavy metal Cd<2+>, the efficient dechlorination effect of tetrachloroethylene is kept, and the Geobacteraceae sulferreducens LYY can achieve the effect of completely decoloring azo dyes AO7; therefore, the Geobacteraceae sulferreducens LYY has a good application prospect in dehalogenation of halogenated organic compounds or preparation of halogenated organic compound dehalogenation microbial inoculum and dye decoloration or preparation of a dye decoloration agent.

The invention relates to a composition for preparing a water body remediation microbial inoculum. The composition comprises a fermentation substrate, first fermentation powder and second fermentationpowder, wherein the first ferment powder comprises bacillus subtilis, saccharomycetes and actinomycetes; wherein the second ferment powder is prepared from bifidobacterium album, compost agrobacteriumtumefaciens and Fluviicola taffensis. The invention also relates to a water body remediation microbial inoculum and a preparation method thereof. According to the composition and the preparation method disclosed by the invention, the fermentation time is shortened, the cost is saved, and the obtained water body remediation microbial inoculum is obviously better in removal of nitrogen and phosphorus as well as organic matters. In the aspects of nitrogen removal and phosphorus removal, the microbial inoculum provided by the invention can remove ammonia nitrogen, total nitrogen and total phosphorus in seriously polluted water more quickly, and finally reaches class II and class III water quality standards. In the aspect of COD removal, the microbial inoculum can treat V-type water to reach the standard of I-type water quality.

The invention relates to the microbiological field, and discloses novel organic halide respiration geobacter and application of the novel organic halide respiration geobacter in bioremediation of chloralkane pollutants. A geobacter IAE bacterial strain is collected in the China General Microbiological Culture Collection Center (culture collection number CGMCC) on July 7, 2020, wherein the addressis No. 3, 1 Courtyard No., Beichenxi Road, Chaoyang District, Beijing City, Institute of Microbiology, Chinese Academy of Sciences, the zip code is 100101, and the collection number is 1.5298.

The invention relates to a heavy metal passivation and soil remediation type microbial agent and a preparation method thereof. The heavy metal passivation and soil remediation type microbial agent contains various microorganisms such as bacillus subtilis, bacillus megatherium, bacillus mucilaginosus, metal reduction geobacter and the like, and is loaded by a composite carrier prepared from an inorganic carrier and an organic carrier. After the heavy metal passivation and soil remediation type microbial agent is applied to soil, the microbial agent can stably survive and reproduce in the carrier, so that heavy metals in the soil can be effectively remedied, and the carrier contains silicon, calcium and magnesium elements, so that the pH value of the soil can be adjusted, and the heavy metals in the soil can be passivated.

The invention discloses a geobacter engineering strain for passivating heavy metals and a construction method of the geobacter engineering strain, the geobacter engineering strain for passivating the heavy metals is Geobacter sulfurreducens HMP-1, and is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO.62189. The invention further discloses a construction method of the geobacter engineering strain for passivating the heavy metals. According to the heavy metal passivated geobacterium engineering strain, cysteine is marked on the pilus surface, and the cysteine in the pilus is combined with heavy metal ions to quickly form metal sulfide precipitates, so that the heavy metal removal rate reaches 70% or above under the condition of not depending on any substrate or highly enriched heavy metal plants, and the heavy metal removal rate can reach 70% or above under the condition of not depending on any substrate or highly enriched heavy metal plants. Therefore, the problem of Cd < 2 + > residue in the environment can be effectively improved, and the defects that the existing microbial remediation effect on heavy metals is unstable and the remediation process is limited by many conditions are overcome.

The invention discloses a method for degrading a protein by co-culture of proteiniphilum acetatigenes and geobacter sulfurreducens. The method comprises the following specific steps: S1, firstly inoculating the proteiniphilum acetatigenes in a culture solution, and then inoculating the geobacter sulfurreducens; S2, protein degradation effect: observing the degradation effect of protein inoculated with mixed bacteria for different days and different in inoculation proportions, and the result shows that the co-culture of the two bacteria is far better than pure culture of the proteiniphilum acetatigenes in terms of protein degradation effect; wherein the proteiniphilum acetatigenes and the geobacter sulfurreducens are inoculated in a proportion of 1: 4, the protein degradation rate of a co-culture system where the proteiniphilum acetatigenes grows for 2 days firstly and then the geobacter sulfurreducens is inoculated is the highest, the degraded proteiniphilum acetatigenes of the invention are protein degradation bacteria and can generate acetic acid, and the acetic acid can be used as a substrate of the sulfur-reducing geobacter and can be used as a substrate of the geobacter sulfurreducens. Compared with other protein degrading bacteria, the mixed system can degrade more proteins, and is simple and convenient to operate.

The invention discloses a sludge fermentation process for pig raising wastewater. A precipitation tank and a fermentation tank are sequentially included from left to right, a feeding pipe is arranged on one side wall of the upper end of the precipitation tank, the lower end of the precipitation tank is communicated with the lower end of the fermentation tank through a material guiding pipe, and a wastewater collecting device is arranged in the precipitation tank. The wastewater collecting device can discharge and collect upper-layer wastewater in the settling tank; and a material passing pipe is arranged on one side wall of the upper end in the fermentation tank, anaerobic sludge particles can be introduced into the material passing pipe, a stirring device is arranged on the fermentation tank, a carbon rod layer is arranged on the stirring device, and the carbon rod layer is electrically connected with an external power supply through a wire. The carbon rod layer is used as a conductor material and intervenes in electron transfer between anaerobic oxidation bacteria and methane bacteria, so that electrons generated by decomposing organic matters by geobacterium are directly utilized by methanogens, carbon dioxide is reduced into methane, the methane yield and the sludge decomposition speed are increased, the sludge treatment efficiency is improved, and the investment is saved.

The invention relates to a microbial cultivation technology, and provides a pure bacteria Geobacter genus microbe electrochemical system capable of being easily started in an aerobic environment. Thesystem takes a moulding carbon electrode material or an electrode material for modifying amorphous carbon as a carrier of an electrochemically active biofilm, and takes a purely cultured Geobacter genus microbe as an inoculum, a culture solution is configured by a PBS solution, a substrate and an aerobic culture medium; the microbe electrochemical system is operated under aerobic conditions, and the headspace oxygen partial pressure is characterized in that PO20 is greater than 0 and less than or equal to 21%; a substrate of the culture solution is acetic acid, ethanol or glucose; an aerobic culture agent is a mixture of one or more of ferric salt, fumarate, hexavalent chromium salt, pentavalent vanadium salt or hexavalent uranium salt. The system uses a method of adding the aerobic culture agent for the first time to aerobic culture of Geobacter genus microbe in the electrochemical system, which is more suitable for practical application, and has the advantages of high safety, universal applicability, and simple operation.