14 results about "Geobacter" patented technology

The invention provides a geotrophic bacterium SG265 and application thereof, and belongs to the technical field of microbial agents. The geotrophic bacteria SG265 disclosed by the invention is preserved in Guangdong Microbial Culture Collection Center on May 7, 2025, the preservation number is GDMCC No: 66266, and the geotrophic bacteria SG265 is named as Geotalea sp. In taxonomy. The terrestrial nutrient bacteria SG265 are anaerobic nitrogen-fixing bacteria, have iron reduction and nitrogen fixation functions at the same time, can reduce nitrogen into nitrogen fertilizer available for crops under the anaerobic condition, can greatly increase the content of available nitrogen in soil, can effectively promote rice growth, can reduce the application amount of the nitrogen fertilizer, can reduce environmental pollution, and can improve the yield of rice. The method has a good application prospect in agricultural production.

The invention provides a complex microbial inoculant and application thereof in degradation of starch and biofuel cells, and belongs to the cross technical field of environmental biotechnology and bioelectrochemistry. According to the invention, a complex microbial inoculant composed of three microorganisms is constructed through a synthetic biological strategy; the complex microbial inoculant comprises genetically engineered Escherichia coli capable of rapidly converting starch into monosaccharide easy to use, shewanella for bearing upstream metabolites and geobacter for generating electricity, wherein the genetically engineered Escherichia coli is used for rapidly converting starch into monosaccharide easy to use; according to the invention, through precise function division of three strains, a complex degradation and power generation process is decomposed into a plurality of sub-steps catalyzed by special strains, so that an efficient'metabolic assembly line 'is formed, the inhibition of intermediate products is reduced, and the overall energy conversion efficiency is improved; the complex microbial inoculant can be used for degrading starch and preparing microbial fuel cells, realizes wastewater purification and recovery of bioelectric energy at the same time, and has remarkable environmental and economic benefits.

This invention belongs to the field of microbiology, specifically relating to a biofilm sensor and a method for detecting biotoxicity using it. The specific technical solution involves a bacterial preparation comprising *Geobacterium*, *Acinetobacter*, and *Pseudomonas*. A sensor is prepared using this bacterial preparation and used to detect the biotoxicity of pollutants. This invention provides a novel biofilm sensor that, based on the high electroactivity of *Geobacterium* and by limiting the amount of carbon source used during cultivation, ultimately achieves an electroactive biofilm with a thickness lower than that of existing technologies, and realizes extremely high detection sensitivity, possessing enormous potential for detection applications.

The present application provides a kind of microbial iron reduction coupling biomass carbon soil carbon sequestration-organic pollution control collaborative remediation method, the present application method is by selecting including nitrogen-fixing bacteria, phosphorus solubilizing bacteria and iron-reducing bacteria Compound bacterial species, mixed with biomass carbon to form microorganism-biomass carbon compound, apply to soil, regulate soil environmental conditions, to promote iron cycle and the carbon sequestration effect of biomass carbon.The specific steps include: selecting nitrogen-fixing bacteria consisting of Clostridium pasteurianum and soybean rhizobium, phosphorus solubilizing bacteria consisting of Bacillus subtilis, flavobacterium or Streptomyces, and iron-reducing bacteria consisting of Geobacter;Prepare biomass carbon;Mix compound bacterial species with biomass carbon;Application and adjust soil pH, moisture content and aeration.The method is suitable for various soils, especially soils susceptible to MCPA pollution, can effectively improve the soil carbon sequestration capacity and reduce environmental pollution.

The application discloses a kind of phthalocyanine iron microwire or nanowire microbial fuel cell anode material preparation and its application.Ordered accumulation nanowire or microwire can be in situ growth on the surface of carbon carrier such as carbon cloth, carbon felt, and phthalocyanine iron molecular accumulation spacing 1.6 nanometer.The application can promote the electron transfer between biological-nonbiological interface, improve the electron transfer rate between electricity-producing microorganism and phthalocyanine iron microwire or nanowire;The application can promote the effective enrichment of electricity-producing bacteria Geobacter in sludge, and the abundance ratio in microbial community is 87.5%, effectively improving the proportion of electricity-producing microorganism;The battery anode of the application has excellent biocompatibility, can guarantee the activity of biofilm in the long-term operation process of microbial fuel cell, can enrich electrically active bacteria, improve the long-term activity of biofilm, promote the interface extracellular electron transfer of microorganism, effectively solve the problem that the output power of microbial fuel cell is low and the long-term operation stability is poor.

This application discloses an in-situ remediation method for uranium contamination and its application. The method involves the interaction between microorganisms and pyrite, driving pyrite hydrolysis, oxidation, atomic rearrangement, and biomineralization. This immobilizes the uranium contaminant within the iron oxide lattice of the reconstructed product, thus remediating the contaminated area. The microorganisms include one or more of Brucella, Geobacterium, or Clostridium. If the uranium-contaminated area contains microorganisms, natural humic substances are used as electron donors to stimulate the in-situ microorganisms. A natural humic substance solution is injected into the uranium-contaminated area via a circulating pumping method to stimulate the in-situ microorganisms. The concentration of the natural humic substance solution is 0.05–5 g / L, and the dosage is 5–500 g / day. This application fully considers and rationally utilizes in-situ mineral, microbial, and geochemical resources, relying on the in-situ mineral and microbial interactions to save engineering costs and achieve high remediation efficiency.

The invention discloses a method for promoting biological nitrogen fixation in a rice field in situ, which does not need to externally inoculate electroactive microorganisms or nitrogen-fixing microorganisms and does not apply an external power supply, and comprises the following steps: S1, carrying out acid treatment and / or hydrophilization treatment on a conductive material; s2, constructing a conductive interface: laying a conductive material on soil of a plowing layer of the rice field and enabling the conductive material to be in close contact with the soil so as to form the conductive interface, and performing waterflooding treatment on the plowing layer of the rice field on which the conductive material is laid; and S3, culturing under a waterflooding anaerobic condition to form a biological membrane on the surface of the conductive interface. According to the method, exogenous electroactive microbial agents or nitrogen-fixing microbial agents do not need to be added, in-situ microorganisms are induced to spontaneously adhere to the surface of the conductive material in the anaerobic environment of the rice field to form a film, community reconstruction is achieved, the relative abundance of electroactive microorganisms such as geobacteria and nitrogen-fixing microorganisms in the surface biological film is improved, the abundance of nitrogen-fixing related genes is increased, and the nitrogen-fixing effect is improved. The rice field biological nitrogen fixation effect is promoted.

The invention belongs to the technical field of plant culture, and particularly relates to a method for improving the yield and / or stress resistance of Antarctic diatom polysaccharide. According to the present invention, it is found that one or more of Alteromonas profundi 1K04570, Pseudoalteromonas atlantica 1C00251, Marinobacter denitrificans 1K05758 and Polaribacter marinaquae 1K00696 are co-cultured with the Antarctic diatom, such that the metabolic pathway of the Antarctic diatom can be activated, the Antarctic diatom can be efficiently stimulated to synthesize the polysaccharide, and the stress resistance of the Antarctic diatom can be synchronously improved; furthermore, by optimizing the inoculation proportion and co-culture conditions, the yield and stress resistance of the Antarctic diatom can be further improved.

The invention relates to a stretchable electrogenesis biological membrane electrode as well as a preparation method and application thereof. The stretchable electrogenesis biological membrane electrode comprises a flexible current collector and a stretchable biological membrane attached to the flexible current collector, the flexible current collector comprises an Au-Ag core-shell structure nanowire and a polymer; the polymer comprises a cross-linked product of polydimethylsiloxane, polymethylhydrosiloxane and a polyethylene glycol-polyethylene oxide-polyethylene glycol block copolymer; the Au-Ag core-shell structure nanowire in the flexible current collector is embedded into the stretchable biological membrane; the stretchable biological membrane comprises geobacter. According to the stretchable electrogenesis biological membrane electrode, the nanowire of an Au-Ag core-shell structure is embedded into the flexible current collector, so that the condition that the resistance change of the flexible current collector is too large in the stretching process is avoided, meanwhile, the action of the biological membrane and the flexible current collector is firm, and an anode has stable electrogenesis performance in the stretching process.

This invention discloses a biofilm-forming microbial consortium with acrylonitrile degradation function, its preparation method, and its application. The microbial consortium consists of *Agrobacterium parakneissus*, *Burkholderia szechuanensis*, *Trichophyton spp.*, *Rhodococcus rubrum*, and *Mycobacterium tumefaciens*; the ratio of viable bacteria of *Burkholderia parakneissus*, *Burkholderia szechuanensis*, *Trichophyton spp.*, *Oligotrophomonas spp.*, and *Trichophyton spp.* is 3:1:1:1:1. This microbial consortium has a stable microbial community structure and can rapidly form a stable biofilm on the surface of biofilter packing material, exhibiting high acrylonitrile degradation efficiency. It is simple to operate, low in cost, and requires only a small amount of additional inorganic nutrients during treatment, without causing secondary pollution, making it an environmentally friendly technology. Its application prospects in the treatment of acrylonitrile-containing wastewater (gas) are broad.

The invention belongs to the technical field of microbial agents, and provides a methyl nutrition complex microbial inoculant and an application thereof in rice methane emission reduction, and the complex microbial inoculant comprises facultative methylobacterium, sphingobacterium, escherichia coli, variovorax, flavobacterium, sphingolipid, pseudoyellow microbacterium, Knoobrucella, sedimentary bacillus and terranean bacillus. The complex microbial inoculant disclosed by the invention can effectively reduce methane emission in rice planting, and has the functions of reducing emission, improving efficiency and promoting stable yield.

The invention discloses an alpha-L-fucosidase mutant and application thereof, and belongs to the technical field of gene engineering and enzyme engineering. According to the alpha-L-fucosidase mutant and the application thereof disclosed by the invention, based on sequence analysis, a geobacterium alpha-L-fucosidase female parent gene is subjected to rational design, primers are designed, and ten mutants are obtained by adopting a site-directed mutagenesis PCR (Polymerase Chain Reaction) technology; the method comprises the following steps of: performing random mutation on a female parent gene by using error-prone PCR (Polymerase Chain Reaction), constructing a random mutation library, performing high-throughput screening on the mutation library, screening six mutation sites capable of improving the heat resistance of alpha-L-fucosidase through two schemes, and integrating the six mutation sites onto a coding sequence through a complete sequence synthesis method, thereby obtaining the alpha-L-fucosidase. And obtaining the combined mutant. The thermal stability of the combined mutant is obviously improved compared with that of a female parent, and the combined mutant shows potential application value in industries such as biological energy, feed, health food and medicine.

The invention discloses a nosZ II type terranean bacillus A33 with N2O reducing capacity and application of the nosZ II type terranean bacillus A33. The preservation number of the Pedobacter sp. A33 is GDMCC No: 66269, and the Pedobacter sp. A33 is preserved in the Guangdong Microbial Culture Collection Center on May 7, 2025, and the preservation number of the Pedobacter sp. A33 is GDMCC No: 66269. The bacterial strain is a new species of terribacter. The novel ternobacter sp. A33 disclosed by the invention has relatively strong N2O reductase activity and also has N2O reducing capacity. Therefore, the novel ternobacter sp. A33 disclosed by the invention has good application potential in the aspect of reducing N2O gas emission.

The application discloses a Pedobacter sp. A33 with N2O reduction capability and an application thereof. The Pedobacter sp. A33 has a preservation number of GDMCC No:66269 and is preserved in the Guangdong Microbial Culture Collection Center on May 7, 2025. The strain is a new species of the Pedobacter genus. The novel Pedobacter sp. A33 has strong N2O reductase activity and N2O reduction capability. Therefore, the novel Pedobacter sp. A33 has good application potential in reducing N2O gas emission.