22 results about "Geobacter" patented technology

The invention provides a carbon-manganese-based composite material as well as a preparation method and application thereof. The carbon-manganese-based composite material is a MnO2-BC composite material formed by MnO2 and biochar, and MnO2 nanoparticles are loaded on the surface of the biochar; by adding 60mg of MnO2-BC / g.VS into a WAS anaerobic fermentation system, the anaerobic fermentation performance of the WAS can be greatly improved, and the degradation rates of polysaccharide, protein and SCOD (soluble chemical oxygen demand) are improved; the method can promote the directional enrichment of Desulfovibrio and Geobacter from the angles of energy metabolism regulation and electron directional transduction optimization, stably and efficiently solves the bottleneck problems of easy acid accumulation, poor flora synergistic effect, easy environmental stress in methane production and the like in the traditional WAS anaerobic fermentation, and has excellent application potential.

The invention discloses an in-situ remediation method for uranium pollution and application of the in-situ remediation method. According to the in-situ remediation method for uranium pollution, pyrite is driven to be hydrolyzed, oxidized, subjected to atomic rearrangement and biomineralized through interaction of microorganisms and pyrite, uranium pollutants are fixedly sealed and stored in reconstructed product iron oxide lattices, uranium pollution areas are remedied, and the microorganisms comprise one or more of brucella, geobacter and clostridium. If the uranium polluted area contains microorganisms, natural humus is used as an electron donor to stimulate in-situ microorganisms. The natural humus solution is injected into the uranium polluted area in a circulating pumping and injecting mode, in-situ microorganisms are stimulated, the concentration of the natural humus solution is 0.05-5 g / L, and the dosage of the natural humus solution is 5-500 g / day. According to the method, in-situ minerals, microorganisms and geochemical resources are fully considered and reasonably utilized, the engineering cost is saved by means of the in-situ occurrence interaction relation of the minerals and the microorganisms, and the remediation efficiency is high.

The invention provides a geotrophic bacterium SG265 and application thereof, and belongs to the technical field of microbial agents. The geotrophic bacteria SG265 disclosed by the invention is preserved in Guangdong Microbial Culture Collection Center on May 7, 2025, the preservation number is GDMCC No: 66266, and the geotrophic bacteria SG265 is named as Geotalea sp. In taxonomy. The terrestrial nutrient bacteria SG265 are anaerobic nitrogen-fixing bacteria, have iron reduction and nitrogen fixation functions at the same time, can reduce nitrogen into nitrogen fertilizer available for crops under the anaerobic condition, can greatly increase the content of available nitrogen in soil, can effectively promote rice growth, can reduce the application amount of the nitrogen fertilizer, can reduce environmental pollution, and can improve the yield of rice. The method has a good application prospect in agricultural production.

The invention provides a complex microbial inoculant and application thereof in degradation of starch and biofuel cells, and belongs to the cross technical field of environmental biotechnology and bioelectrochemistry. According to the invention, a complex microbial inoculant composed of three microorganisms is constructed through a synthetic biological strategy; the complex microbial inoculant comprises genetically engineered Escherichia coli capable of rapidly converting starch into monosaccharide easy to use, shewanella for bearing upstream metabolites and geobacter for generating electricity, wherein the genetically engineered Escherichia coli is used for rapidly converting starch into monosaccharide easy to use; according to the invention, through precise function division of three strains, a complex degradation and power generation process is decomposed into a plurality of sub-steps catalyzed by special strains, so that an efficient'metabolic assembly line 'is formed, the inhibition of intermediate products is reduced, and the overall energy conversion efficiency is improved; the complex microbial inoculant can be used for degrading starch and preparing microbial fuel cells, realizes wastewater purification and recovery of bioelectric energy at the same time, and has remarkable environmental and economic benefits.

This invention belongs to the field of microbiology, specifically relating to a biofilm sensor and a method for detecting biotoxicity using it. The specific technical solution involves a bacterial preparation comprising *Geobacterium*, *Acinetobacter*, and *Pseudomonas*. A sensor is prepared using this bacterial preparation and used to detect the biotoxicity of pollutants. This invention provides a novel biofilm sensor that, based on the high electroactivity of *Geobacterium* and by limiting the amount of carbon source used during cultivation, ultimately achieves an electroactive biofilm with a thickness lower than that of existing technologies, and realizes extremely high detection sensitivity, possessing enormous potential for detection applications.

The present application provides a kind of microbial iron reduction coupling biomass carbon soil carbon sequestration-organic pollution control collaborative remediation method, the present application method is by selecting including nitrogen-fixing bacteria, phosphorus solubilizing bacteria and iron-reducing bacteria Compound bacterial species, mixed with biomass carbon to form microorganism-biomass carbon compound, apply to soil, regulate soil environmental conditions, to promote iron cycle and the carbon sequestration effect of biomass carbon.The specific steps include: selecting nitrogen-fixing bacteria consisting of Clostridium pasteurianum and soybean rhizobium, phosphorus solubilizing bacteria consisting of Bacillus subtilis, flavobacterium or Streptomyces, and iron-reducing bacteria consisting of Geobacter;Prepare biomass carbon;Mix compound bacterial species with biomass carbon;Application and adjust soil pH, moisture content and aeration.The method is suitable for various soils, especially soils susceptible to MCPA pollution, can effectively improve the soil carbon sequestration capacity and reduce environmental pollution.

The application discloses a kind of phthalocyanine iron microwire or nanowire microbial fuel cell anode material preparation and its application.Ordered accumulation nanowire or microwire can be in situ growth on the surface of carbon carrier such as carbon cloth, carbon felt, and phthalocyanine iron molecular accumulation spacing 1.6 nanometer.The application can promote the electron transfer between biological-nonbiological interface, improve the electron transfer rate between electricity-producing microorganism and phthalocyanine iron microwire or nanowire;The application can promote the effective enrichment of electricity-producing bacteria Geobacter in sludge, and the abundance ratio in microbial community is 87.5%, effectively improving the proportion of electricity-producing microorganism;The battery anode of the application has excellent biocompatibility, can guarantee the activity of biofilm in the long-term operation process of microbial fuel cell, can enrich electrically active bacteria, improve the long-term activity of biofilm, promote the interface extracellular electron transfer of microorganism, effectively solve the problem that the output power of microbial fuel cell is low and the long-term operation stability is poor.

The invention provides a soil carbon sequestration-organic pollution control synergistic remediation method by coupling microbial iron reduction with biomass charcoal, which comprises the following steps: selecting a composite strain comprising nitrogen-fixing bacteria, phosphate solubilizing bacteria and iron reducing bacteria, mixing the composite strain with biomass charcoal to form a microbial-biomass charcoal compound, applying the microbial-biomass charcoal compound to soil, regulating and controlling soil environmental conditions, and controlling organic pollution. The iron circulation and the carbon sequestration effect of the biomass charcoal are promoted. The method comprises the following specific steps: selecting nitrogen-fixing bacteria consisting of clostridium pasteurianum and rhizobium japonicum, phosphate solubilizing bacteria consisting of bacillus subtilis, flavobacterium or streptomyces, and iron reducing bacteria consisting of geobacterium; preparing biomass charcoal; mixing the composite strain with the biomass charcoal; and applying and adjusting the pH value, the moisture content and the air permeability of the soil. The method is suitable for various kinds of soil, especially soil easily polluted by MCPA, the carbon sequestration capacity of the soil can be effectively improved, and environmental pollution is reduced.

The present invention discloses a microbial remediation agent for water vanadium pollution control and a preparation method thereof, comprising microorganisms and a carrier, wherein the microorganisms include denitrifying Thiobacillus and sulfur-reducing Geobacter, the carrier includes activated carbon, sulfur powder and sodium acetate, and the microbial remediation agent is obtained by loading the microorganisms onto the carrier. The present invention targets V(V) in groundwater, and uses a microbial agent to reduce V(V) in groundwater into V(IV) precipitation, so as to reduce the toxicity and mobility of vanadium in groundwater, and significantly reduce the content of highly toxic V(V). The microbial preparation has high activity, fast reproduction, environmental protection and no pollution, low cost, and reasonable compatibility between denitrifying Thiobacillus and Geobacter, symbiotic coordination, and neither the problem of eutrophication caused by the introduction of a large amount of organic nutrients nor the generation of a large amount of sulfate acidification of groundwater.

This application discloses an in-situ remediation method for uranium contamination and its application. The method involves the interaction between microorganisms and pyrite, driving pyrite hydrolysis, oxidation, atomic rearrangement, and biomineralization. This immobilizes the uranium contaminant within the iron oxide lattice of the reconstructed product, thus remediating the contaminated area. The microorganisms include one or more of Brucella, Geobacterium, or Clostridium. If the uranium-contaminated area contains microorganisms, natural humic substances are used as electron donors to stimulate the in-situ microorganisms. A natural humic substance solution is injected into the uranium-contaminated area via a circulating pumping method to stimulate the in-situ microorganisms. The concentration of the natural humic substance solution is 0.05–5 g / L, and the dosage is 5–500 g / day. This application fully considers and rationally utilizes in-situ mineral, microbial, and geochemical resources, relying on the in-situ mineral and microbial interactions to save engineering costs and achieve high remediation efficiency.

The invention discloses a method for promoting biological nitrogen fixation in a rice field in situ, which does not need to externally inoculate electroactive microorganisms or nitrogen-fixing microorganisms and does not apply an external power supply, and comprises the following steps: S1, carrying out acid treatment and / or hydrophilization treatment on a conductive material; s2, constructing a conductive interface: laying a conductive material on soil of a plowing layer of the rice field and enabling the conductive material to be in close contact with the soil so as to form the conductive interface, and performing waterflooding treatment on the plowing layer of the rice field on which the conductive material is laid; and S3, culturing under a waterflooding anaerobic condition to form a biological membrane on the surface of the conductive interface. According to the method, exogenous electroactive microbial agents or nitrogen-fixing microbial agents do not need to be added, in-situ microorganisms are induced to spontaneously adhere to the surface of the conductive material in the anaerobic environment of the rice field to form a film, community reconstruction is achieved, the relative abundance of electroactive microorganisms such as geobacteria and nitrogen-fixing microorganisms in the surface biological film is improved, the abundance of nitrogen-fixing related genes is increased, and the nitrogen-fixing effect is improved. The rice field biological nitrogen fixation effect is promoted.

The invention belongs to the technical field of plant culture, and particularly relates to a method for improving the yield and / or stress resistance of Antarctic diatom polysaccharide. According to the present invention, it is found that one or more of Alteromonas profundi 1K04570, Pseudoalteromonas atlantica 1C00251, Marinobacter denitrificans 1K05758 and Polaribacter marinaquae 1K00696 are co-cultured with the Antarctic diatom, such that the metabolic pathway of the Antarctic diatom can be activated, the Antarctic diatom can be efficiently stimulated to synthesize the polysaccharide, and the stress resistance of the Antarctic diatom can be synchronously improved; furthermore, by optimizing the inoculation proportion and co-culture conditions, the yield and stress resistance of the Antarctic diatom can be further improved.

The invention relates to a stretchable electrogenesis biological membrane electrode as well as a preparation method and application thereof. The stretchable electrogenesis biological membrane electrode comprises a flexible current collector and a stretchable biological membrane attached to the flexible current collector, the flexible current collector comprises an Au-Ag core-shell structure nanowire and a polymer; the polymer comprises a cross-linked product of polydimethylsiloxane, polymethylhydrosiloxane and a polyethylene glycol-polyethylene oxide-polyethylene glycol block copolymer; the Au-Ag core-shell structure nanowire in the flexible current collector is embedded into the stretchable biological membrane; the stretchable biological membrane comprises geobacter. According to the stretchable electrogenesis biological membrane electrode, the nanowire of an Au-Ag core-shell structure is embedded into the flexible current collector, so that the condition that the resistance change of the flexible current collector is too large in the stretching process is avoided, meanwhile, the action of the biological membrane and the flexible current collector is firm, and an anode has stable electrogenesis performance in the stretching process.

The invention relates to a method for in-situ treatment of a uranium-polluted water body and application. A method for in-situ treatment of a uranium-polluted water body comprises the following steps that a bacterial suspension is prepared, one or more of brucella, geobacterium, clostridium or shewanella is selected as a strain, the bacterial suspension is cultured to the logarithmic phase and resuspended and trained, the bacterial suspension is obtained, and the final concentration of the bacterial suspension is 1 * 10 < 6 >-1 * 10 < 9 > CFU / mL; clay minerals are selected from one or more of kaolinite, montmorillonite, illite, smectite, chlorite, sepiolite, glauconite or talc, and the concentration of the clay turbid liquid is 0.01-10 g / L; and synchronously adding the bacterial suspension and the clay suspension into the uranium-polluted water body. Through clay mineral-microorganism interaction, uranium elements are promoted to be embedded between clay layers and then reduced, uranium pollution is fixed between the clay layers and isolated from the external environment, and uranium pollution solidification is truly achieved.

This invention discloses a biofilm-forming microbial consortium with acrylonitrile degradation function, its preparation method, and its application. The microbial consortium consists of *Agrobacterium parakneissus*, *Burkholderia szechuanensis*, *Trichophyton spp.*, *Rhodococcus rubrum*, and *Mycobacterium tumefaciens*; the ratio of viable bacteria of *Burkholderia parakneissus*, *Burkholderia szechuanensis*, *Trichophyton spp.*, *Oligotrophomonas spp.*, and *Trichophyton spp.* is 3:1:1:1:1. This microbial consortium has a stable microbial community structure and can rapidly form a stable biofilm on the surface of biofilter packing material, exhibiting high acrylonitrile degradation efficiency. It is simple to operate, low in cost, and requires only a small amount of additional inorganic nutrients during treatment, without causing secondary pollution, making it an environmentally friendly technology. Its application prospects in the treatment of acrylonitrile-containing wastewater (gas) are broad.

The invention belongs to the technical field of microbial agents, and provides a methyl nutrition complex microbial inoculant and an application thereof in rice methane emission reduction, and the complex microbial inoculant comprises facultative methylobacterium, sphingobacterium, escherichia coli, variovorax, flavobacterium, sphingolipid, pseudoyellow microbacterium, Knoobrucella, sedimentary bacillus and terranean bacillus. The complex microbial inoculant disclosed by the invention can effectively reduce methane emission in rice planting, and has the functions of reducing emission, improving efficiency and promoting stable yield.

Provided are a microorganism which is a Geobacter sp. strain 60473; a microbial fuel cell composition containing the Geobacter sp. strain 60473 and an organic substance; a microbial fuel cell in which the Geobacter sp. strain 60473 is used to decompose the organic substance as a fuel; a microbial electrolysis cell composition containing the Geobacter sp. strain 60473 and the organic substance; and a microbial electrolysis cell in which the Geobacter sp. strain 60473 is used to decompose the organic substance as the fuel.

The invention discloses an alpha-L-fucosidase mutant and application thereof, and belongs to the technical field of gene engineering and enzyme engineering. According to the alpha-L-fucosidase mutant and the application thereof disclosed by the invention, based on sequence analysis, a geobacterium alpha-L-fucosidase female parent gene is subjected to rational design, primers are designed, and ten mutants are obtained by adopting a site-directed mutagenesis PCR (Polymerase Chain Reaction) technology; the method comprises the following steps of: performing random mutation on a female parent gene by using error-prone PCR (Polymerase Chain Reaction), constructing a random mutation library, performing high-throughput screening on the mutation library, screening six mutation sites capable of improving the heat resistance of alpha-L-fucosidase through two schemes, and integrating the six mutation sites onto a coding sequence through a complete sequence synthesis method, thereby obtaining the alpha-L-fucosidase. And obtaining the combined mutant. The thermal stability of the combined mutant is obviously improved compared with that of a female parent, and the combined mutant shows potential application value in industries such as biological energy, feed, health food and medicine.

The invention discloses a preparation method of a ditch filling material for road drainage purification and nourishment, wherein medical stone is crushed and sequentially added into a calcium chloride solution and a sodium silicate solution for soaking treatment, washed and dried and then heat-treated to obtain modified medical stone; agricultural waste is made into activated carbon; iron tailings sand is ground and sterilized to make a microbial culture medium, inoculated with sulfur-reducing Geobacter and metal-reducing Geobacter and then cultured for 5-7 days to obtain a hybrid substance containing ferrous iron, which is uniformly mixed with modified medical stone, apatite, activated carbon and fine sand, and 15%-25% of water is added and stirred to obtain. Compared with the prior art, the ditch filling material can not only efficiently adsorb and remove organic and inorganic pollutants in road water through redox reaction on the basis of fully meeting drainage needs, but also release phosphorus in the water body, effectively slow down the diffusion of pollutants, significantly reduce the negative impact on the natural environment, and promote the growth and development of roadside vegetation.

The invention discloses a nosZ II type terranean bacillus A33 with N2O reducing capacity and application of the nosZ II type terranean bacillus A33. The preservation number of the Pedobacter sp. A33 is GDMCC No: 66269, and the Pedobacter sp. A33 is preserved in the Guangdong Microbial Culture Collection Center on May 7, 2025, and the preservation number of the Pedobacter sp. A33 is GDMCC No: 66269. The bacterial strain is a new species of terribacter. The novel ternobacter sp. A33 disclosed by the invention has relatively strong N2O reductase activity and also has N2O reducing capacity. Therefore, the novel ternobacter sp. A33 disclosed by the invention has good application potential in the aspect of reducing N2O gas emission.

The application discloses a Pedobacter sp. A33 with N2O reduction capability and an application thereof. The Pedobacter sp. A33 has a preservation number of GDMCC No:66269 and is preserved in the Guangdong Microbial Culture Collection Center on May 7, 2025. The strain is a new species of the Pedobacter genus. The novel Pedobacter sp. A33 has strong N2O reductase activity and N2O reduction capability. Therefore, the novel Pedobacter sp. A33 has good application potential in reducing N2O gas emission.

The invention discloses a preparation method of a side ditch filler for road drainage purification and cultivation, which comprises the following steps: crushing medical stone, sequentially adding the crushed medical stone into a calcium chloride solution and a sodium silicate solution for soaking treatment, cleaning, drying, and carrying out heat treatment to obtain modified medical stone; agricultural wastes are prepared into activated carbon; the preparation method comprises the following steps: grinding iron tailing sand, sterilizing, preparing into a microbial culture medium, inoculating sulfur-reducing geobacter and metal-reducing geobacter, culturing for 5-7 days to prepare a ferrous-containing hybrid substance, uniformly mixing the ferrous-containing hybrid substance with the modified medical stone, apatite, activated carbon and fine sand, adding 15-25% of water, and uniformly stirring to obtain the composite material. Compared with the prior art, on the basis of fully meeting the drainage requirement, the side ditch filler not only can efficiently adsorb and remove organic and inorganic pollutants in road accumulated water through the oxidation-reduction effect, but also can release phosphorus elements in a water body, effectively slow down diffusion of the pollutants and remarkably reduce the negative influence on the natural environment; and the growth and development of roadside vegetation are promoted.