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Pure bacteria Geobacter genus microbe electrochemical system capable of being easily started in aerobic environment

A microbial electrochemical and oxygen environment technology, applied in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve toxic and side effects, affect the growth and adsorption of pure bacteria Geobacter microorganisms, and affect microbial Growth and other problems, to achieve the effect of reducing toxicity, reducing production, and easy operation

Inactive Publication Date: 2019-10-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these oxygen reducing agents are mainly sulfur-containing compounds. On the one hand, excessive oxygen reducing agents may have toxic side effects on the microorganisms of the genus Geobacter and affect the growth of microorganisms; on the other hand, these sulfur-containing compounds are oxidized to form The elemental sulfur is easily adsorbed on the electrode surface, which affects the growth and adsorption of pure bacteria Geobacter microorganisms on the electrode surface

Method used

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  • Pure bacteria Geobacter genus microbe electrochemical system capable of being easily started in aerobic environment
  • Pure bacteria Geobacter genus microbe electrochemical system capable of being easily started in aerobic environment

Examples

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specific Embodiment 1

[0024] Graphite flakes were used as carriers, and ferric salt was added to cultivate Geobacter microorganisms.

[0025] Set the surface area to 1.5cm 2 The high-temperature pyrolytic graphite plate was polished to smooth with P400 sandpaper, P800 sandpaper and 0.05 μm aluminum powder in sequence. After ultrasonication for 10 min in 70% ethanol aqueous solution, it was washed and dried with deionized water as the carrier of electrochemically active biofilm. Geobacter sulfurreducens PCA was used as the inoculation source, 14mL sterile PBS solution, 1.5g / L sodium acetate and 20mM ferric citrate were added to the culture solution, and the headspace gas in the reactor was replaced so that the oxygen partial pressure was 1%. The Geobacter can be started by applying a DC power supply to both ends of the battery with a voltage of 0.7V. As a reference comparison, the difference of the traditional method is to add 14mL sterile PBS solution and 1.5g / L sodium acetate to prepare the cultu...

specific Embodiment 2

[0030] The microorganisms of the genus Geobacter were cultured by adding fumarate with graphite rod as the carrier.

[0031] Set the surface area to 1.5cm 2 Graphite rods were polished to smooth with P400 sandpaper, P800 sandpaper and 0.05 μm aluminum powder in sequence, ultrasonicated in 70% ethanol aqueous solution for 10 min, washed with deionized water and dried as the carrier of electrochemically active biofilm. Geobacter metallireducens was used as the inoculation source, 14mL sterilized PBS solution, 1.5g / L ethanol and 20mM sodium fumarate were added to the culture solution, and the headspace gas in the reactor was replaced to make the oxygen partial pressure 5%. Geobacter microorganisms can be started by applying a DC power supply to both ends of the battery with a voltage of 0.3V.

specific Embodiment 3

[0033] Using carbon cloth as a carrier, adding sodium dichromate to cultivate Geobacter microorganisms.

[0034] Set the surface area to 1.5cm 2Carbon cloth was sonicated in 70% ethanol aqueous solution for 10 minutes, soaked for 1 day, washed with deionized water, and treated at 370°C for 1 hour, as a carrier of electrochemically active biofilm. Geobacter metallireducens was used as the inoculation source, 14mL sterilized PBS solution, 1.5g / L ethanol and 20mM sodium dichromate were added to the culture solution, and the headspace gas in the reactor was replaced so that the oxygen partial pressure was 21%. Geobacter can be started by applying a voltage of 1V to both ends of the battery with a DC power supply.

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Abstract

The invention relates to a microbial cultivation technology, and provides a pure bacteria Geobacter genus microbe electrochemical system capable of being easily started in an aerobic environment. Thesystem takes a moulding carbon electrode material or an electrode material for modifying amorphous carbon as a carrier of an electrochemically active biofilm, and takes a purely cultured Geobacter genus microbe as an inoculum, a culture solution is configured by a PBS solution, a substrate and an aerobic culture medium; the microbe electrochemical system is operated under aerobic conditions, and the headspace oxygen partial pressure is characterized in that PO20 is greater than 0 and less than or equal to 21%; a substrate of the culture solution is acetic acid, ethanol or glucose; an aerobic culture agent is a mixture of one or more of ferric salt, fumarate, hexavalent chromium salt, pentavalent vanadium salt or hexavalent uranium salt. The system uses a method of adding the aerobic culture agent for the first time to aerobic culture of Geobacter genus microbe in the electrochemical system, which is more suitable for practical application, and has the advantages of high safety, universal applicability, and simple operation.

Description

technical field [0001] The invention relates to a microorganism cultivation technology, in particular to a method for starting a pure bacteria Geobacter microbial electrochemical system in an aerobic environment. The method can be applied to soil remediation, heavy metal recovery, sewage treatment, etc., and belongs to the fields of microbiology and bioelectrochemistry. Background technique [0002] Recovering valuable substances while treating wastewater is of great significance to alleviate the energy crisis and environmental pollution problems, and meets the major needs of the country. Microbial electrochemical systems (MESs) use the electrochemically active microorganisms attached to the anode to oxidize the organic matter in the wastewater, and use the cathode to accept the electrons generated by the anode, so as to not only treat wastewater but also recover biomass energy, and realize the process of wastewater treatment from energy consumption to production capacity. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/42
CPCC12M25/02C12M35/02
Inventor 成少安羊家威
Owner ZHEJIANG UNIV
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