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Geobacteraceae sulferreducens Geo-LYY strain for dehalogenation of halogenated organic compounds and dye decoloration and application thereof

A technology for decolorization of halogenated organic compounds and dyes, which is applied in the field of Geo-LYY strains of Geobacterial bacteria for dehalogenation of halogenated organic compounds and decolorization of dyes, can solve the problems of insignificant dehalogenation effect, etc. In situ microbial remediation ability and the effect of improving groundwater quality and environment

Active Publication Date: 2020-06-12
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defects and deficiencies that the dehalogenation effect of the existing halogenated organic substances is not significant, to use Geobacter to carry out the environmental condition tolerance of halogenated organic substances in the application of environmental remediation, and to explore the method of compound pollution remediation The method provides a theoretical basis, and provides a Geobacter Geo-LYY strain for dehalogenation of halogenated organic matter and decolorization of dyes and its application

Method used

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  • Geobacteraceae sulferreducens Geo-LYY strain for dehalogenation of halogenated organic compounds and dye decoloration and application thereof
  • Geobacteraceae sulferreducens Geo-LYY strain for dehalogenation of halogenated organic compounds and dye decoloration and application thereof
  • Geobacteraceae sulferreducens Geo-LYY strain for dehalogenation of halogenated organic compounds and dye decoloration and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The acquisition of embodiment 1 ground bacterium (Geobacter lovleyi) LYY

[0034] 1) Isolation and cultivation of strains

[0035] 1. Experimental method

[0036] Sediment samples were collected from the black and odorous water body in Tangxia City, Guangzhou City, Guangdong Province, and stored at 4°C. After the sediment samples were stirred evenly, 5 mL was inoculated into the anaerobic dechlorination liquid medium (the formulation of which was PCE as the only electron acceptor) As shown in Table 1), cultured at 30°C while monitoring the dechlorination of PCE; after three generations of enrichment culture, a high-efficiency bacterial group with complete dechlorination ability to PCE was obtained; by controlling the passage time, the enrichment After collecting, adding antibiotics to kill miscellaneous bacteria and continuous gradient dilution and purification operations, a purified bacterial strain was obtained, and the purified bacterial strain was named strain LYY....

Embodiment 2

[0061] Example 2 Tolerance of Geobacter LYY under PCE substrate concentration pressure and environmental condition pressure

[0062] 1. Experimental method

[0063] Geobacter LYY was inoculated in the PCE dechlorinated culture solution, and the substrate (PCE) concentration pressure and environmental conditions (antibiotics, heavy metal Cu 2+ and Cd 2+ ) Tolerance determination under pressure, concrete experimental method and experimental result are as follows:

[0064] (1) Geobacter LYY tolerance PCE concentration experiment: Under the conditions of acetate concentration of 10mM, Geobacter LYY inoculation amount of 2.5%, and culture temperature of 30°C, the PCE concentration was set to 0.25mM, 0.5mM, 1mM, 2mM , 3mM, 5mM, to investigate the dechlorination of Geobacter LYY to PCE.

[0065] (2) Antibiotic resistance experiment of Geobacter LYY: Under the conditions of acetate concentration of 10mM, PCE concentration of 1mM, Geobacter LYY inoculation amount of 2.5%, and cultur...

Embodiment 3

[0071] The mensuration of embodiment 3 Geobacter LYY dye decolorization ability

[0072] Geobacter LYY was inoculated in the anaerobic medium (its formula is shown in Table 3) with azo dye as the only electron donor without reducing agent, and the live bacteria decolorization ability test of azo dye was carried out. The specific experimental method And the experimental results are as follows:

[0073] 1. Experimental method

[0074] Under the conditions of 10mM acetic acid concentration, 2.5% Geobacter LYY inoculum, and 30°C culture temperature, 60ppm of azo dye AO7 was set as the only electron acceptor, and a blank sample without strain inoculation was used as a reference under the same conditions. , the standard curve was measured by spectrophotometry, and the decolorization of the azo dye AO7 by Geobacter LYY was determined.

[0075] Table 3 does not contain the anaerobic liquid medium formula of reducing agent

[0076]

[0077]

[0078] 2. Experimental results

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Abstract

The invention discloses a Geobacteraceae sulferreducens Geo-LYY strain for dehalogenation of halogenated organic compounds and dye decoloration and application of the Geobacteraceae sulferreducens Geo-LYY strain. According to the invention, a strain of Geobacteraceae sulferreducens LYY with halogenated organic compound dehalogenation and dye decolorization capabilities is obtained through screening and separation; the strain was preserved in Guangdong Microbial Culture Collection Center on February 17, 2020, and the preservation number of the strain is GDMCC No: 61003. The Geobacteraceae sulferreducens LYY can tolerate the pressure of environmental conditions of high-concentration tetrachloroethylene, antibiotics, heavy metal Cu<2+> and heavy metal Cd<2+>, the efficient dechlorination effect of tetrachloroethylene is kept, and the Geobacteraceae sulferreducens LYY can achieve the effect of completely decoloring azo dyes AO7; therefore, the Geobacteraceae sulferreducens LYY has a good application prospect in dehalogenation of halogenated organic compounds or preparation of halogenated organic compound dehalogenation microbial inoculum and dye decoloration or preparation of a dye decoloration agent.

Description

technical field [0001] The invention belongs to the technical field of microbiology. More specifically, it relates to a Geobacter Geo-LYY strain for dehalogenating organic matter and decolorizing dyes and its application. Background technique [0002] As a commonly used organic solvent, perchlorethylene (PCE) is widely used as metal degreasing detergent, dry cleaning agent, general solvent and organic synthesis intermediate. Due to the volatile nature of tetrachlorethylene and improper handling during production, storage and use, a large amount of tetrachlorethylene enters the environment and pollutes the atmosphere, surface water, soil and groundwater. Perchlorethylene will enter the soil due to dumping, leakage, or rainwater runoff, and part of it will even leak into groundwater, resulting in groundwater pollution. Pollution of tetrachlorethylene and trichlorethylene in groundwater is especially common; at the same time Perchlorethylene has strong environmental toxicity ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/36C02F101/38
CPCC12N1/20C02F3/34C02F2101/308C02F2101/36C02F2101/38C12R2001/01C12N1/205
Inventor 汪善全梁咏颐吴日枫
Owner SUN YAT SEN UNIV
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